1.The synergistic effect of amygdalin and HSYA on the IL-1beta induced endplate chondrocytes of rat intervertebral discs.
Kai NIU ; Yong-Jian ZHAO ; Lei ZHANG ; Chen-Guang LI ; Yong-Jun WANG ; Wei-Chao ZHENG
Acta Pharmaceutica Sinica 2014;49(8):1136-1142
The effect of amygdalin joint hydroxysafflor yellow A (HSYA) on the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and the possible mechanism were studied and explored. Chondrocytes were obtained from endplate of one-month SD rat intervertebral discs and cultured primary endplate chondrocytes. After identification, they were divided into normal group, induced group, amygdalin group, HSYA group and combined group. CCK-8 kit was adopted to detect the proliferation of the endplate chondrocytes. FCM was measured to detect the apoptosis. Real-time PCR method was adopted to observe the mRNA expression of Aggrecan, Col 2 alpha1, Col 10 alpha1, MMP-13 and the inflammatory cytokines IL-1beta. The protein expression of Col II, Col X was tested through immunofluorescence. Compared with the normal group, the proliferation of the endplate chondrocytes decreased while the apoptosis increased (P < 0.05). With down regulation of the mRNA expressions of Aggrecan, Col 2 alpha1 and up regulation of the mRNA expressions of Col 10 alpha1, MMP-13, IL-1beta (P < 0.05), the protein expression of Col II decreased while the protein expression of Col X increased. Compared with the induced group, amygdalin group, HSYA group, the combined group could inhibit the apoptosis and promote the proliferation (P < 0.05). They could increase the mRNA expressions of Aggrecan and Col 2 alpha1 while decrease the mRNA expressions of Col 10 alpha1, MMP-13 and IL-1beta (P < 0.05). They could also enhance the protein expression of Col II while reduce the protein expression of Col X. The effect of the combined group was significantly better than that of amygdalin and HSYA. Amygdalin joint HSYA could inhibit the degeneration of the endplate chondrocytes derived from intervertebral discs of rats induced by IL-1beta and better than the single use of amygdalin or HSYA.
Amygdalin
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pharmacology
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Animals
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Apoptosis
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Cells, Cultured
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Chalcone
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analogs & derivatives
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pharmacology
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Chondrocytes
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drug effects
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Collagen
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metabolism
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Drug Synergism
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Interleukin-1beta
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Intervertebral Disc
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cytology
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Quinones
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pharmacology
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Rats
2.Protective effect of hydroxysafflor yellow A against rat cortex mitochondrial injuries induced by cerebral ischemia.
Jing-wei TIAN ; Feng-hua FU ; Wang-lin JIANG ; Chao-yun WANG ; Fang SUN ; Tai-ping ZHANG
Acta Pharmaceutica Sinica 2004;39(10):774-777
AIMTo study the effects of hydroxysafflor yellow A (HSYA) on the mitochondrial function of cortex mitochondrial during cerebral ischemia in rats.
METHODSRat focal cerebral ischemia model in rats was established by ligation of middle cerebral central artery. Cortex mitochondria were isolated and prepared for the measurement of membrane fluidity, swelling, respiratory function, activities of mitochondrial respiratory enzymes and superoxide dismutase (SOD), contents of phospholipid, malondial dehyde (MDA) and Ca2+ to evaluate the function of mitochondria.
RESULTSFocal cerebral ischemia resulted in severe neuronal mitochondrial injuries, which could be alleviated by i.v. HSYA (10, 20 mg x kg(-1)), and nimodipine (Nim, 1.0 mg x kg(-1)). The swelling of mitochondria was ameliorated, the decomposability of membrane phospholipid was decreased, the membrane fluidity of mitochondria was increased, HSYA also significantly inhibited the decrease in the activities of respiratory enzymes and SOD of mitochondria, and the increase in MDA and Ca2+ levels caused by cerebral ischemia in rats.
CONCLUSIONHSYA showed a protective action against the cortex mitochondrial injuries in rats induced by cerebral ischemia. The mechanisms may be derived from reducing lipid peroxides, inhibiting Ca2+ overload, scavenging free radicals and improving the energy metabolism.
Animals ; Brain Ischemia ; metabolism ; pathology ; Calcium ; metabolism ; Chalcone ; analogs & derivatives ; pharmacology ; Male ; Malondialdehyde ; metabolism ; Membrane Fluidity ; drug effects ; Mitochondria ; metabolism ; pathology ; Mitochondrial Swelling ; drug effects ; NAD ; metabolism ; Neuroprotective Agents ; pharmacology ; Quinones ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism
3.Benzylideneacetophenone derivatives attenuate IFN-gamma-induced IP-10/CXCL10 production in orbital fibroblasts of patients with thyroid-associated ophthalmopathy through STAT-1 inhibition.
Sung Hee LEE ; Seul Ye LIM ; Ji Ha CHOI ; Jae Chul JUNG ; Seikwan OH ; Koung Hoon KOOK ; Youn Hee CHOI
Experimental & Molecular Medicine 2014;46(6):e100-
The aim of the present study was to identify a new candidate anti-inflammatory compound for use in the active stage of thyroid-associated ophthalmopathy (TAO). Benzylideneacetophenone compound JC3 [(2E)-3-(4-hydroxy-3-methoxyphenyl)phenylpro-2-en-l-one] was synthesized based on a structural modification of yakuchinone B, a constituent of the seeds of Alpinia oxyphylla, which belongs to the ginger family (Zingiberaceae), has been widely used in folk medicine as an anti-inflammatory phytochemical. Orbital fibroblasts were primarily cultured from patients with TAO, and the potential of JC3 to suppress the interferon (IFN)-gamma-induced protein (IP)-10/CXCL10 production in these cells was determined. IFN-gamma strongly increased the level of IP-10/CXCL10 in orbital fibroblasts from patients with TAO. JC3 exerted a significant inhibitory effect on the IFN-gamma-induced increase in IP-10/CXCL10 in a dose-dependent manner; its potency was greater than that of an identical concentration of yakuchinone B with no toxicity to cells at the concentration range used. Moreover, the constructed dimer and trimer polystructures of JC3, showed greater potency than JC3 in suppressing the IFN-gamma-induced production of IP-10/CXCL10. JC3 significantly attenuated the IP-10/CXCL10 mRNA expression induced by IFN-gamma, and a gel-shift assay showed that JC3 suppressed IFN-gamma-induced DNA binding of signal transducer and activator of transcription-1 (STAT-1) in TAO orbital fibroblasts. Our results provide initial evidence that the JC3 compound reduces the levels of IP-10/CXCL10 protein and mRNA induced by IFN-gamma in orbital fibroblasts of TAO patients. Therefore, JC3 might be considered as a future candidate for therapeutic application in TAO that exerts its effects by modulating the pathogenic mechanisms in orbital fibroblasts.
Cells, Cultured
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Chalcone/chemical synthesis/*pharmacology
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Chemokine CXCL10/genetics/*metabolism
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Diarylheptanoids/chemistry/pharmacology
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Fibroblasts/*drug effects/metabolism
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Graves Ophthalmopathy/*metabolism
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Humans
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Interferon-gamma/*metabolism
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Orbit/cytology
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RNA, Messenger/genetics/metabolism
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STAT1 Transcription Factor/genetics/*metabolism
4.Safflower Yellow Compounds Alleviate Okadaic Acid-Induced Impairment of Neurite Outgrowth in Differentiated SH-SY5Y Cells.
Zhen Hua WANG ; Xiao Bing SHI ; Gang LI ; Xue Yan HAO ; Zhen Zhen YUAN ; Xiao Hai CAO ; Hong Lun WANG ; Ji LI ; Cheng Jun MA
Biomedical and Environmental Sciences 2020;33(10):812-816
5.C-ring cleavage of liquiritigenin extracted from licorice roots by an oxygen-tolerant bovine rumen bacterium strain Aeroto-Niu-O16.
Ming WANG ; Hui ZHAO ; Xiu-Ling WANG ; Hong-Lei ZHANG ; Qing-Hong HAO
Acta Pharmaceutica Sinica 2012;47(5):664-669
Aeroto-Niu-O16, an oxygen-tolerant bovine rumen bacterium, is capable of aerobically reducing isoflavones daidzein and genistein to dihydrodaidzein and dihydrogenistein through catalytic hydrogenation. In this study, it was found that bacterium strain Aeroto-Niu-O16 was able to cleavage the C-ring of liquiritigenin (LG), which is one of the main biologically active components of licorice roots, in the presence of atmospheric oxygen. LG was prepared by acid hydrolysis of the crude extract of licorice roots. The metabolite of LG obtained in strain Aeroto-Niu-O16 was identified as davidigenin (DG) based on the data of UV, MS, 1H and 13C NMR. The maximal concentration of LG that the strain Aeroto-Niu-O16 was able to transform effectively was 0.8 mmol x L(-1) and the average productivity of the metabolite DG was 71.7%. Furthermore, when 0.1% (m/v) of L-cysteine or sodium thiosulfate was added in the cultural medium, the average bioconversion rate of LG was increased from 71.7% to 78.3% and 77.2%, respectively. The in vitro antioxidant investigation showed that 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of DG was significantly or extremely significantly higher than that of LG at the concentrations from 0.2 mmol x L(-1) to 1.6 mmol x L(-1). We discoverd for the first time that LG can be converted to DG, which has stronger and wider biological activities, through microbial biotransformation method.
Animals
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Antioxidants
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isolation & purification
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metabolism
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pharmacology
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Bacteria, Anaerobic
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isolation & purification
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metabolism
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Biotransformation
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Biphenyl Compounds
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metabolism
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Cattle
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Chalcone
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analogs & derivatives
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metabolism
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pharmacology
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Cysteine
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pharmacology
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Flavanones
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isolation & purification
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metabolism
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pharmacology
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Glycyrrhiza
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chemistry
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Picrates
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metabolism
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Plant Roots
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chemistry
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Plants, Medicinal
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chemistry
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Rumen
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microbiology
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Thiosulfates
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pharmacology
6.Separation and evaluation of antioxidant constituents from Carthamus tinctorius.
Shi-Jun YUE ; Yu-Ping TANG ; Lin-Yan WANG ; Hao TANG ; Shu-Jiao LI ; Pei LIU ; Shu-Lan SU ; Jin-Ao DUAN
China Journal of Chinese Materia Medica 2014;39(17):3295-3300
Bio-active components from Carthamus tinctorius were separated on the basis of antioxidant capacities in vitro. The antioxidant capacity was investigated on the basis of the ability to scavenge DPPH radical, ABTS radical and reduce Fe3+ of different polar fractions. Furthermore, the chemical compounds were isolated from bio-active fraction, and were evaluated for the antioxidative effects. Five major components were isolated and identified from water extract as 6-hydroxykaempferol 3,6,7-tri-O-β-D-glucoside(1), 6-hydroxykaempferol 3-O-β-rutinoside-6-O-β-D-glucoside (2), 6-hydroxykaempferol 3-O-β-D-glucoside (3), hydroxysafflor yellow A (4) and anhydrosafflor yellow B (5). By evaluating and comparing the antioxidative effects of different fractions and obtained compounds, the results showed that water extract displayed significantly high antioxidative activities and 6-hydroxykaempferol glycosides and quinochalcone C-glycosides were found as main contribution for antioxidant property.
Antioxidants
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isolation & purification
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metabolism
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pharmacology
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Benzothiazoles
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metabolism
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Biphenyl Compounds
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metabolism
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Carthamus tinctorius
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chemistry
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Chalcone
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analogs & derivatives
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isolation & purification
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metabolism
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pharmacology
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Ferric Compounds
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metabolism
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Free Radicals
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metabolism
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Kaempferols
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isolation & purification
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metabolism
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pharmacology
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Oxidation-Reduction
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drug effects
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Picrates
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metabolism
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Plant Extracts
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isolation & purification
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metabolism
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pharmacology
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Plants, Medicinal
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chemistry
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Quinones
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isolation & purification
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metabolism
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pharmacology
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Sulfonic Acids
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metabolism
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Water
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chemistry
7.Protective effect of hydroxysafflor yellow A on experimental cerebral ischemia in rats.
Hai-bo ZHU ; Zhen-hua WANG ; Jing-wei TIAN ; Feng-hua FU ; Ke LIU ; Chang-ling LI
Acta Pharmaceutica Sinica 2005;40(12):1144-1146
AIMTo investigate the protective effect of hydroxysafflor yellow A (HSYA), a soluble element extracted from Carthamus tinctorius L., on focal cerebral ischemia in rats.
METHODSFocal cerebral ischemia in male Wistar-Kyoto (WKY) rats were induced by permanent middle cerebral artery occlusion (MCAO). Three doses of 1.5, 3.0 and 6.0 mg x kg(-1) of HSYA were administrated to three groups of rats, separately, via sublingular vein injection 30 min after the onset of ischemia. 24 h after ischemia in rats, neurological deficit scores were evaluated and the infarction area of brain was assessed by quantitative image analysis. The in vitro neuroprotective effect of HSYA was tested in cultured fetal cortical neurons exposed to glutamate and sodium cyanide (NaCN).
RESULTSHSYA at doses of 3.0 and 6.0 mg x kg(-1) exerted significant neuroprotective effects on rats with focal cerebral ischemic injury as expressed by neurological deficit scores and reduced the infarct area as compared with saline group, and the potency of HSYA at dose of 6.0 mg x kg(-1) was similar to that of 0.2 mg x kg(-1) of nimodipine. In vitro studies, HSYA significantly inhibited neurons damage induced by exposure to glutamate and NaCN in cultured fetal cortical cells.
CONCLUSIONHSYA has potential neuroprotective action against focal cerebral ischemia in rats and cultured rat fetal cortical neurons as well.
Animals ; Behavior, Animal ; drug effects ; Brain ; pathology ; Brain Ischemia ; etiology ; pathology ; physiopathology ; Carthamus tinctorius ; chemistry ; Cells, Cultured ; Cerebral Cortex ; cytology ; Chalcone ; analogs & derivatives ; isolation & purification ; pharmacology ; Glutamic Acid ; Infarction, Middle Cerebral Artery ; complications ; L-Lactate Dehydrogenase ; metabolism ; Male ; Neurons ; cytology ; metabolism ; Neuroprotective Agents ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Quinones ; isolation & purification ; pharmacology ; Rats ; Rats, Inbred WKY ; Sodium Cyanide ; antagonists & inhibitors
8.Effect of safflor yellow B on vascular endothelial cells injury induced by angiotensin-II.
Chao-Yun WANG ; Shu-Ping ZHANG ; Yong XU ; Ming YANG ; Wen-Guo JIANG ; Hai-Yun LUAN
Acta Pharmaceutica Sinica 2012;47(6):811-815
This study is to investigate protective effect of safflor yellow B (SYB) against vascular endothelial cells (VECs) injury induced by angiotensin-II (Ang-II). VECs were cultured and divided into six groups: control group, Ang-II group, Ang-II + SYB (1 micromolL(-1)) group, Ang-II + SYB (10 micromolL(-1)) group, Ang-II + SYB (100 micromolL(-1)) group and Ang- II + verapamil (10 micromolL(-1)) group. Except control group, all of VECs in other groups were treated with Ang- II at the final concentration of 0.1 micromolL(-1). Mitochondria membrane potential (MMP) and free calcium concentration ([Ca2+]i) were measured by laser scanning confocal microscopy, and mitochondria complex IV activity was detected by BCA method. The levels of reactive oxygen species (ROS) in VECs were analyzed by fluorescence detector and apoptosis of VECs was observed by flow cytometer. Caspase 3 was determined by Western blotting method. Comparing with control group, Ang-II was able to increase [Ca2+]i and ROS level, decrease MMP level, inhibit complex IV activity and enhance caspase 3 activity in VECs, as a result, enhance apoptosis of VECs. But SYB could significantly reduce the result induced by Ang- II relying on different dosages (P < 0.05 or P < 0.01). SYB was able to eliminate the effect of Ang-II on VECs via regulating [Ca2+]i, mitochondrial structure and function and inhibiting apoptosis.
Angiotensin II
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adverse effects
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Antioxidants
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isolation & purification
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pharmacology
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Apoptosis
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drug effects
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Calcium
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metabolism
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Carthamus tinctorius
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chemistry
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Caspase 3
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metabolism
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Cells, Cultured
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Chalcone
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analogs & derivatives
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isolation & purification
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pharmacology
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Drugs, Chinese Herbal
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isolation & purification
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pharmacology
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Electron Transport Complex IV
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metabolism
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Endothelial Cells
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cytology
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metabolism
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Humans
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Membrane Potential, Mitochondrial
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drug effects
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Mitochondrial Proton-Translocating ATPases
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metabolism
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Plants, Medicinal
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chemistry
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Reactive Oxygen Species
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metabolism
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Vasoconstrictor Agents
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adverse effects
9.Study on the antioxidative effect of Safflor Yellow.
Ming JIN ; Jin-rong LI ; Wei WU
China Journal of Chinese Materia Medica 2004;29(5):447-449
OBJECTIVETo observe the antioxidative effect of Safflor Yellow (SY).
METHODHydroxyl radical scavenge effect of SY was tested with 1,10-phenanthroline-Fe2+ oxidative assay. Lipid peroxidation of mouse liver suspension was measured with thiobarbituric acid colorimetry technique. Hemocytocatheresis was determined with colorimetry.
RESULTHydroxyl radical could be scavenged by 1.39 to 3.42 g x L(-1) SY dose dependently. Mouse liver suspension peroxidation was inhibited by 77.8 to 776.1 mg x L(-1) SY dosage dependently. Hemocytocatheresis was attenuated by 37.1 to 297.1 mg x L(-1) SY dose dependently.
CONCLUSIONSY is an antioxidative part of Carthamus tinctorius.
Animals ; Antioxidants ; isolation & purification ; pharmacology ; Carthamus tinctorius ; chemistry ; Chalcone ; administration & dosage ; analogs & derivatives ; isolation & purification ; pharmacology ; Dose-Response Relationship, Drug ; Flowers ; chemistry ; Free Radical Scavengers ; pharmacology ; Hydroxyl Radical ; metabolism ; Lipid Peroxidation ; drug effects ; Liver ; metabolism ; Male ; Mice ; Plants, Medicinal ; chemistry ; Rabbits
10.Effect of Hydroxy Safflower Yellow A on glucocorticoid-induced bone marrow mesenchymal stem cells osteogenic differentiation.
Tian WAN ; Min-Rui WU ; Zhen-Xi QI
China Journal of Orthopaedics and Traumatology 2014;27(3):224-228
OBJECTIVETo observe the effect of Hydroxy Safflower Yellow A (HSYA) on the expression of osteogenic markers, such as alkaline phosphatase, Cbf(alpha)l and type I collagen, and explore the mechanism of HSYA in the prevention and treatment of glucocorticoid-induced ischemic necrosis of femoral head.
METHODSFifteen healthy and adult New Zealand white rabbits were collected and weighted 0.9 to 1.3 kg. The rabbits were injected abdominally with anesthetic drugs, then received marrow cavity puncture of tibia and anterior superior iliac spine to get bone marrow blood. Rabbits bone marrow mesenchymal stem cells (BMSCs) were separated from the bone marrow blood, cultured in vitro and passaged. The 3rd generation of BMSCs which had good growth condition were randomly divided into blank group, model group and HSYA groups with different doses. The BMSCs in model group were treated with high dose of dexamethasone to induce adipogenic differentiation of cells cultured in vitro, and inhibit osteogenic differentiation. The BMSCs in HSYA groups received high dose of dexamethasone and different concentrations of HSYA simultaneously. The blank group received not any special handling. After a week,the expressions of alkaline phosphatase, Cbf(alpha)l and type I collagen mRNA were detected.
RESULTSThe alkaline phosphatase activity was significantly decreased in BMSCs of the model group as compared with the blank group (P < 0.01), and the expression of Cbf(alpha)l and type I collagen mRNA were also decreased significantly (P<0.01). The alkaline phosphatase activity was significantly increased in BMSCs of each HSYA group as compared with the model group (P < 0.05 or P < 0.01), and the expression of Cbf(alpha)l and type I collagen mRNA were also increased significantly (P < 0.05 or P < 0.01).
CONCLUSIONThe mechanism of HSYA may be related to the effect of antagonism to the reduced osteogenic differentiation induced by glucocorticoid.
Alkaline Phosphatase ; genetics ; metabolism ; Animals ; Bone Marrow Cells ; cytology ; drug effects ; metabolism ; Cell Differentiation ; drug effects ; Cells, Cultured ; Chalcone ; analogs & derivatives ; chemistry ; pharmacology ; Collagen Type I ; genetics ; metabolism ; Core Binding Factor alpha Subunits ; genetics ; metabolism ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Female ; Glucocorticoids ; pharmacology ; Male ; Mesenchymal Stromal Cells ; cytology ; drug effects ; metabolism ; Osteogenesis ; drug effects ; Rabbits