1.Evaluation of SD BIOLINE Chagas Ab Rapid Kit.
Mi Jung JI ; Jae Sang NOH ; Byung Ki CHO ; Young Shik CHO ; Sun Joo KIM ; Byoung Su YOON
The Korean Journal of Laboratory Medicine 2009;29(1):48-52
BACKGROUND: Chagas' disease is caused by Trypanosoma cruzi, a protozoan parasite, which is transmitted by blood-sucking bugs or through blood transfusion or organ transplantation. It is endemic in Central and South America. The objective of this study was to compare the performance of immunochromatographic SD Bioline Chagas Ab Rapid (Standard Diagnostics, Korea) with three immunochromatographic kits for the detection of antibodies to T. cruzi. METHODS: A total of 320 serum specimens (140 positive and 180 negative) from National Reference Laboratory for Chagas and Leishmaniasis (NRLCL, Honduras) were used for the evaluation of four different test kits: SD Bioline Chagas Ab Rapid, Chagas Stat-Pak Assay (Chembio Diagnositc Systems, USA), OnSite Chagas Ab Rapid test-Cassette (CTK Biotech, USA), and Trypanosoma Detect Rapid Test (InBios International, USA). The results of four kits were compared with those of NRLCL. Cross-reactivity with other parasites was also evaluated. RESULTS: Compared with the results of NRLCL, sensitivity and specificity were 99.3% and 100% for both of SD and Chembio kits, 97.2% and 100% for InBios kit, and 97.9% and 98.8% for CTK kit. None of other parasites showed cross-reactivity. CONCLUSIONS: SD Bioline Chagas Ab Rapid kit showed test results highly correlating with those of National Reference Laboratory for Chagas and Leishmaniasis. It can be used for a rapid detection of Chagas' disease in endemic region and monitoring the disease among overseas travelers in Korea.
Animals
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Antibodies, Protozoan/*blood/immunology
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Blood Specimen Collection
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Chagas Disease/*diagnosis/immunology/parasitology
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Chromatography/methods
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Humans
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*Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Trypanosoma cruzi/*immunology
2.Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms.
Danielle KIAN ; Cesar Armando Contreras LANCHEROS ; Igor Alexandre Campos DAMIANI ; Tamiris Zanforlin Olmos FERNANDES ; Phileno PINGE-FILHO ; Marcia Regina Machado DOS SANTOS ; Jose Franco DA SILVEIRA ; Celso Vataru NAKAMURA ; Joao Santana DA SILVA ; Sueli Fumie YAMADA-OGATTA ; Lucy Megumi YAMAUCHI
The Korean Journal of Parasitology 2015;53(4):483-488
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
Amino Acid Sequence
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Chagas Disease/*parasitology
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Gene Expression Regulation, Developmental
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Humans
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Life Cycle Stages
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Molecular Sequence Data
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Protozoan Proteins/*genetics/metabolism
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Sequence Alignment
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Trypanosoma cruzi/*genetics/*growth & development/isolation & purification/metabolism