Since the publication of the first edition of the Encyclopedia of Applied Ethics in 1998 , the world has changed .The post-millennium and post 9/11 context brought with it a shift of emphasis concerning the re-sponse to terrorism , for example , including growing interest in measures such as biometric identification technolo-gies.But it is not only in the socio -political context that we see a change .In science the sequencing of the human genome , the promise of stem cell science and the rise of synthetic biology have all led to considerable debate .Ethi-cal issues also increasingly have a global dimension .The second edition of the Encyclopedia of Applied Ethics in-volved some issues that not or little mentioned in the 1998 version.Neverthless, it still reserved those classicpa-pers and updated when necessary , to reflect the progress of applied ethical approaches .Here, I tried to draw the outline of the main trends and ways of debates during this time .I think these ways can be divided into three catego-ries, due to the changes in contexts , issues and ethical approaches .