To determine the dose-urvival and repair characteristics of the jejunal crypt cells, experimental study was carried out using total 70 mice. Single or split irradiations of 1,100 to 2,200 rad were delivered to whole bodies of C57 BL mice, using a cesium 137 animal irradiator and those mice were sacrificed after 90 hours. The number of regenerating crypts per jejunal circumference was counted by a jejunal crypt cell assay technique and dose-esponse curve was measured. The results were as follows : 1. The average number of jejunal crypts per circumference in control group was 140. In a single irradiation group, the number of regenerated jejunal crypts was, 125, 56, 2 in each subgroup of 1,100 rad, 1,400 rad and 1,800 rad respectively. In split irradiation group, it was 105, 44, 2 in each subgroup of 1,400rad 1,800rad and 2,200 rad respectively. 2. Mean lethal dose of mouse jejunal crypt cell was 167 and 169 rad respectively in a single and split irradiation. 3. Repair dose of sublethal damage was 280 rad. 4. sublethal damage was completely repaired within 4 hours between the split dose of irradiation.
Animals ; Cesium ; Mice* ; Radiation Effects*

This paper presents dose distributions in water around Fletcher-Suit colpostat containing 137Cs tube, and shielding effect of internal lead shield. Using ready packed film, author measured dose distribution in water around the colpostat containing cesium source. Nine sheets of films on one side of the colpostat are packed with acryl frames cut out so as to fill water, and irradiated in water by cesium source in the colpostat. Dose distributions on transverse plane and upper plane 0.5 cm from upper surface of the colpostat were measured. Shielding effect was greater in upper medial direction than in lower medial direction. And that was the greatest around 30degree from the axis of the colpostat on upper side and around 50degree on lower side. In the region 7 cm from the center of the colpostat, shielding efficiency was 0.23 to 0.35 on the lower 50degree and 0.26 to 0.42 on the upper 30degree, and decreased with increase of distance.
Axis, Cervical Vertebra ; Cesium ; Water

To determine the radiosensitivity and dose-urvival characteristics of jejunal crypt cells, experimental study was done using total 40 mice. Single irradiation of 1,000rad to 1,600rad was delivered to whole bodies of mice, using a cesium 137 animal irradiator. The number of regenerating crypts per jejunal circumference was counted, by using a jejunal crypt cell assay technique, and dose response curve was measured. The average number of jejunal crypt per circumference in control group was 140+/-10. Mean lethal dose(D0) of mouse jejunal crypt cell was 135rad.
Animals ; Cesium ; Jejunum* ; Mice* ; Radiation Tolerance*

We developed a high-resolution micro-CT system based on rotational gantry and flat-panel detector for live mouse imaging. This system is composed primarily of an x-ray source with micro-focal spot size, a CMOS (complementary metal oxide semiconductor) flat panel detector coupled with CsI (Tl) (thallium-doped cesium iodide) scintillator, a linearly moving couch, a rotational gantry coupled with positioning encoder, and a parallel processing system for image data. This system was designed to be of the gantry-rotation type which has several advantages in obtaining CT images of live mice, namely, the relative ease of minimizing the motion artifact of the mice and the capability of administering respiratory anesthesia during scanning. We evaluated the spatial resolution, image contrast, and uniformity of the CT system using CT phantoms. As the results, the spatial resolution of the system was approximately the 11.3 cycles/mm at 10% of the MTF curve, and the radiation dose to the mice was 81.5 mGy. The minimal resolving contrast was found to be less than 46 CT numbers on low-contrast phantom imaging test. We found that the image non-uniformity was approximately 70 CT numbers at a voxel size of ~55x55x100micrometer3. We present the image test results of the skull and lung, and body of the live mice.
Anesthesia ; Animals ; Artifacts ; Cesium ; Lung ; Mice ; Skull

Bombs ; Cesium ; poisoning ; Humans ; Polonium ; poisoning ; Radiation Injuries ; Terrorism

Recently, the incidence of pulmonary embolism (PE) after surgery began to increase in Japan and to prevent PE has become essentially important. During the period between July 2003 and August 2004, we placed 203 general surgical patients under our perioperative management using intermittent pneumatic compression (IPC) and compression stockings (CS). We evaluated the effect of our management on the prevention of postoperative PE in those patients. The incidence of PE, prognosis, complications, patient's complaints, cost-benefit were examined. No fatal PE occurred. One patient with low SpO2 had a chest pain and dyspnea but pulmonary scintigrams revealed no PE. Two other patients had contact dermatitis by CS and another patient using an epidural catheter suffered temporary paraplegia after heparin injection. The government has approved a fee for PE prophylaxis since April 2004. Our management using IPC and CS for PE prophylaxis after surgery proved to be an effective in reducing the risk of PE. However, we must take the atmost care in injecting heparin into patients with epidural catheters.
cisplatin/etoposide protocol ; Pulmonary Embolism ; Postoperative Period ; Patients ; Cesium

From Nov. 1983 through Feb. 1986, 35 patients of uterine cervical cancer were treated by external radiation therapy and intracavitary radiation therapy using Fletcher-Sult-Delclos applicator. Age of the patients ranged from 32 to 70 years (median age:53 years). All patients had follow up from 9 to 34 months and median follow up of 20 months. 4 patients were in stage I, 25 were in stage II, 5 were in stage III and I was in stage IV. Overall regression rate was 80% and uncorrected actuarial 2 year survival rate was 88%. The incindence of rectal complications were analyzed. There was no rectal complication in the patients who received less than 7000 rad maximal rectal dose, but 2 out of 17 patients who received more than 7000rad developed moderate degree (grade 2) of rectal complication. In viewing of our results, Fletcher-Suit-Delclos applicator (3M) seemed to be an appropriate instrument for intracavitary radiation therapy in the patients of uterine cervical cancer.
Cesium* ; Follow-Up Studies ; Humans ; Radiotherapy* ; Survival Rate ; Uterine Cervical Neoplasms*

The importance of recombinant adenoviral vectors for the development of gene therapy and prophylactic and therapeutic vaccines has led to efforts for process development of large scale production of clinically safe adenoviral vectors. First of all, cell lines producing replication incompetent adenoviral vectors required for clinical application have been developed and the concept of banking and characterization of cell lines and adenoviral vectors has been established. In order to meet the need of amount of adenoviral vectors for clinical trials, various large scale suspension culture methods using serum-free media have been developed along with development of large scale purification methods using chromatography instead of cesium chloride method. In addition, methods for the quality control of adenoviral vectors have been established and applied for the clinical lots.
Cell Line ; Cesium ; Chlorides ; Chromatography ; Culture Media, Serum-Free ; Genetic Therapy ; Quality Control ; Vaccines

PURPOSE: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, particularly on the expression of osteocalcin and osteopontin. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.5, 1, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. After the specimens were harvested, RNA was extracted on the 3rd, 7th, 14th, and 21st day after irradiation. The RNA strands were reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR. RESULTS: The irradiated cells demonstrated a dose-dependent increase in osteocalcin and a dose-dependent decrease in osteopontin mRNA expression compared with the non-irradiated control group. The amount of osteocalcin mRNA expression decreased significantly at the 3rd day after irradiation of 0.5, 1, 4, and 8 Gy, and also decreased significantly at the 3rd, 14th, and 21st day after irradiation in the 8 Gy exposed group compared with the control group. The degree of osteopontin mRNA expression increased significantly at the 7th day after irradiation of 0.5, 1, 4, and 8 Gy. CONCLUSION: These results showed that each single dose of 0.5, 1, 4, and 8 Gy influenced the mRNA expression of osteocalcin and osteopontin associated with the calcification stage of osteoblastic cells, suggesting that each single dose affected bone formation at the cell level.
Cell Line* ; Cesium ; DNA, Complementary ; Osteoblasts* ; Osteocalcin* ; Osteogenesis ; Osteopontin* ; Polymerase Chain Reaction ; RNA ; RNA, Messenger*

The action of opioid on the hyperpolarization-activated cation current (Ih) in substantia gelatinosa neurons were investigated by using whole-cell voltage-clamp recording in rat spinal brain slices. Hyperpolarizing voltage steps revealed slowly activating currents in a subgroup of neurons. The half-maximal activation and the reversal potential of the current were compatible to neuronal Ih. DAMGO (1 muM), a selective-opioid agonist, reduced the amplitude of Ih reversibly. This reduction was dose-dependent and was blocked by CTOP (2 muM), a selective mu-opioid antagonist. DAMGO shifted the voltage dependence of activation to more hyperpolarized potential. Cesium (1 mM) or ZD 7288 (100 muM) blocked Ih and the currents inhibited by cesium, ZD 7288 and DAMGO shared a similar time and voltage dependence. These results suggest that activation of mu-opioid receptor by DAMGO can inhibit Ih in a subgroup of rat substantia gelatinosa neurons.
Animals ; Brain ; Cesium ; Enkephalin, Ala(2)-MePhe(4)-Gly(5)- ; Neurons* ; Rats* ; Substantia Gelatinosa*