1.A comparative study on inducing non-homologous mesenchymal stem cells to differentiate into neural stem cells using non-homologous cerebrospinal fluid.
Chao REN ; Xiaoyun LIU ; Meirong WAN ; Deqin GENG ; Wei GE ; Jinmei LI ; Weiwei ZHANG
Journal of Biomedical Engineering 2013;30(6):1290-1297
In order to set up a base for stem cells to be widely used in clinical medicine, we tried to optimize, in this study, the technique that induces human mesenchymal stem cells (hMSCs) to differentiate into neural stem cells by using cerebrospinal fluid (CSF) from the different groups. After the induction, presence of neural stem cells was confirmed with microscope observation, flow cytometry analysis, immunohistochemistry and fluorescent immunohistochemistry. At the same time, we also compared and analysed the data of the number of stem cells when it totally met the requirements for clinical treatment and the days required. At last, we confirmed that hMSCs could be induced to differentiate into neural stem cells, and that the number of cells totally met the requirements for clinical treatment. But there were some differences both in the number of cells and the days required. Among the groups, the group that marrow mesenchymal stem cells from patients own induced by CSF from healthy volunteers used the shortest time and the quantity of the cells was significantly higher than those of the others.
Cell Differentiation
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Cerebrospinal Fluid
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chemistry
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Culture Media
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chemistry
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Flow Cytometry
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Humans
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Immunohistochemistry
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Mesenchymal Stromal Cells
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cytology
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Neural Stem Cells
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cytology
2.Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings.
Byeong Teck KANG ; Jong Hwan LEE ; Dong In JUNG ; Chul PARK ; Su Hyun GU ; Hyo Won JEON ; Dong Pyo JANG ; Chae Young LIM ; Fu Shi QUAN ; Young Bo KIM ; Zang Hee CHO ; Eung Je WOO ; Hee Myung PARK
Journal of Veterinary Science 2007;8(4):369-376
The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.
Animals
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Behavior, Animal/physiology
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Brain/metabolism/pathology
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Cerebral Infarction/*etiology/*pathology
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Cerebrospinal Fluid/chemistry/cytology
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Disease Models, Animal
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*Dogs
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Infarction, Middle Cerebral Artery/*complications/*pathology
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Magnetic Resonance Imaging
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Male