1.Analysis of multiple cephalosporins in blood and urine by HPLC.
Mu LI ; Lin-Chuan LIAO ; You-Yi YAN ; Lin YANG ; Xiao-Min LIU ; Jin DAI ; Xiao-Na MA ; Xin SHENG ; Hong HUANG ; Gui SU
Journal of Forensic Medicine 2009;25(6):437-439
OBJECTIVE:
To establish a new high performance liquid chromatography (HPLC) method for determining the concentration of cefazolin, cefradine, cefoperazone and cefotaxime in blood and urine, as well as to investigate its applicability.
METHODS:
Protein in blood and urine was precipitated directly by acetonitrile with acetanilide was used as the internal standard using Agilent Zorbax SB-Aq column (250 mm x 4.6 mm, 5 microm). The mixed solvents of water (triethylamine 0.12%, acetic acid 0.12%) and acetonitrile were used as the mobile phase to separate cephalosporins using gradient elution method at 1 mL/min (flow rate) and 254 nm (detection wavelength).
RESULTS:
The working curve of four cephalosporins showed a good correlation (r = 0.9993), with the detection limit up to 0.01 microg/mL. The recovery rate was more than 81.2%.
CONCLUSION
This method is fast, easy and accurate. It is suitable for biological analysis of the 4 cephalosporins of the blood and urine in practical cases.
Adult
;
Anti-Bacterial Agents/urine*
;
Cefazolin/urine*
;
Cefoperazone/urine*
;
Cefotaxime/urine*
;
Cephalosporins/urine*
;
Cephradine/urine*
;
Chromatography, High Pressure Liquid/methods*
;
Forensic Toxicology
;
Humans
;
Male
;
Sensitivity and Specificity
;
Specimen Handling