OBJECTIVE: This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by cephalosporins in the umbilical venous and placenta of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the umbilical venous plasma and placental tissue homogenates of normal pregnancy (n=12) and preeclampsia (n=12) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After samples of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or cephalothin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Protein carbonyls formation by moxalactam and cephalothin in the umbilical venous plasma and of women with preeclampsia were significantly higher than that of women with normal pregnancy (8.5+/-2.0 vs. 6.6+/-1.4 nmol/mg protein, p<0.05, 7.6+/-1.6 vs. 6.2+/-1.2 nmol/mg protein, p<0.05). Protein carbonyls formation by moxalactam and cephalothin in the placental tissue homogenates of women with preeclampsia were significantly higher than that of women with normal pregnancy (17.6+/-5.3 vs. 13.0+/-4.2 nmol/mg protein, p<0.05, 16.1+/-5.2 vs. 12.5+/-4.4 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and cephalosporins induced protein carbonyls levels of umbilical venous plasma, and placental tissue homogenates (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in placenta may be involved in the pathogenesis of preecalmpsia.
Cephalosporins ; Cephalothin ; Female ; Humans ; Moxalactam ; Placenta* ; Plasma* ; Pre-Eclampsia* ; Pregnancy ; Protein Carbonylation

Seventy cases of empyema treated on the pediatric Service of Kwangju Christian Hospital during 9 years 6 months from Jan. 1970 to Jun. 1979 were analyzed and summerized as follows : 1. Males outnumbered females 38 to 32. No seasonal difference in prevalence was notes. 2. About 60% of empyema(41 cases) were on the right side and 36% on the left side. In 3 cases both sides were affected. The most common concurrent disease was pneumonia, which was suspected as th etiology of empyema. 3. Common chief complaints were dyspnea(53%), fever(47%) and cough(43%). 4. Upon culture of pus, 21 cases among 63 cases(33%) showed no bacterial growth, indicating that had under gone antibacterical treatment before admission. 5. The most common causative organism was Staphylococcus aureus(41.2%) followed vy Pseudomonas(7.9%) Streptococcus(4.8%) E.Coli(4.8%) and Pneumococcus(4.8%). 6. Upon sensitivity test, no resistance was observed to Methicillin and Amikacin, but most of the causative agents were sensitive to Gentamicin, Cephalothin and Novobiocin, while they were least sensitive to Penicillin(17%) and colimycin(9%). 7. Average hospital days were 18, and two cases(2.9%) expired, both one year of age. Average duration of closed drainage was two weeks.
Amikacin ; Cephalothin ; Drainage ; Empyema* ; Female ; Gentamicins ; Gwangju ; Humans ; Male ; Methicillin ; Novobiocin ; Pneumonia ; Prevalence ; Seasons ; Staphylococcus ; Suppuration

PURPOSE: By identifying micro-organism in postoperative patients, we evaluated problems associated with the use of cephalosporin for the prevention of infection, and antibiotic choice in cases, in which causative micro-organisms cannot be islolated. MATERIALS AND METHODS: Eighteen patients, from January 1998 to May 2002, who received arthroplasty, were studied. All cases were administered preoperative first generation cephalosporin one hour before operation. All patients with infection had micro-organisms identified received an antibiotic sensitivity test. RESULTS: Gram positive cocci were identified in fifteen cases (83%) (staphylococcus was the most common (72%)), and there were eight cases (80%) in acute infections and seven (88%) in chronic. 78-83% were sensitive to vancomycin, teicoplanin and ciprofloxacin, but only 17% were sensitive to cephalothin. CONCLUSION: Staphylococcus was found to be the most common organism in postoperative infection, and ciprofloxacin was more effective than cephalosporin. Ciprofloxacin is considered to be an effective antibiotic in patients with unidentified causative organisms.
Arthroplasty ; Bacteria* ; Cephalothin ; Ciprofloxacin ; Gram-Positive Cocci ; Humans ; Staphylococcus ; Teicoplanin ; Vancomycin

PURPOSE: The present study aims to determine the common pathogens involved in the etiology of bacterial keratitis and to analyze not only the antibiotic susceptibility of bacterial isolates from patients with bacterial keratitis but also the propriety of initial treatment. METHODS: A retrospective study of 161 eyes in 161 patients with bacterial keratitis, who were diagnosed by cultures from 2000 to 2011, was performed. Causative bacteria and antibiotic susceptibility were evaluated in consecutive 6-year periods. RESULTS: The most common bacteria was the pseudomonas species in the 2 time periods tested and cefazolin with tobramycin was the most commonly used antibiotic (125 cases, 77.6%) for initial treatment of bacterial keratitis. In vitro testing showed gram-negative susceptibility to tobramycin and ciprofloxacin was over 70%, gram-positive susceptibility to cephalothin and vancomycin was 100% and there was no significant difference between the 2 time periods tested. Eight cases had resistance to the initial treatment, but only 1 case showed treatment failure. CONCLUSIONS: Antibiotics used for initial treatment of bacterial keratitis were able to obtain a proper effect but several cases showed bacterial resistance to antibiotics. Thus, continued testing is essential to monitor for antibiotic resistance.
Anti-Bacterial Agents* ; Bacteria ; Cefazolin ; Cephalothin ; Ciprofloxacin ; Drug Resistance, Microbial ; Humans ; Keratitis* ; Pseudomonas ; Retrospective Studies ; Tobramycin ; Treatment Failure ; Vancomycin

To investigate the pathogenicity, genomic pattern, and o-like hemolysin of Staphylococcus lugdunensis (S. lugdunensis) in acute oral infection, S. lugdunensis was isolated from patients with an acute oral infection and from healthy persons. Antibiotic susceptibility, in vitro cellular toxicity, in vivo virulence, and hemolytic activity were tested, and plasmid DNA and restriction pattern of whole genomic DNA were analyzed to characterize the staphylococci. The dot blot and Southern blot hybridization analysis of staphylococcal DNA were performed with o-hemolysin gene probe. The isolation ratio of S. lugdunensis in the patients was higher than that in the healthy persons. S. lugdunensis from the patients with an acute oral infection showed resistance to penicillin, ampicillin, methicillin, cephalothin, and clindamycin. In the analysis of plasmid, there was a clear band about 6.5 kb in three strains of S. lugdunensis isolated from the patients with infection. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had o-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with Staphylococcus aureus (S. aureus) on sheep blood agar plates. In the analysis of genomic pattern, four strains of S. lugdunensis that gave synergistic hemolysis with S. aureus showed a similar genetic pattern with HindIII enzyme digests. In dot blot analysis, all strains of S. lugdunensis showed a positive reaction with the probe of 5-hemolysin gene in S. aureus. In Southern blot analysis, a 7.3 kb HindIII fragment was observed in DNA of S. lugdunensis that gave synergistic hemolysis with S. aureus, and a 2.5 kb band was observed in HindIII digests of S. aureus in the patients. These results suggest that S. lugdunensis may be an important pathogen in an acute oral infection and the 7.3 kb HindIII fragment from S. lugdunensis DNA may contain o-like hemolysin gene.
Agar ; Ampicillin ; Blotting, Southern ; Cephalothin ; Clindamycin ; DNA ; Erythrocytes ; Hemolysis ; Humans ; Methicillin ; Penicillins ; Plasmids ; Sheep ; Staphylococcus aureus ; Staphylococcus lugdunensis* ; Staphylococcus* ; Virulence

Neonatal and Infantile conjunctivitis are common disease in ophthalmologic outpatient department but, a study of causative organisms and antibiotics sensitivity tests are insufficient till now We divided 117 babies(117eyes) having conjunctivitis into neonatal and infantile group who were visited Sung-Ae General Hospital from Jan, 1989 to May, 1990. We performed bacterial culture and antibiotic sensitivity test. The results were as follows; 1) Culture positive rates are 65% in neonates and 87% in infants. There is no sexual difference of ratio. 2) The incidence of isolated organisms is Staphylococcus aureus, Coagulase negative staphylococcus and Streptococcus. 3) Cephalothin is most sensitive antibiotics and Chloramphenicol shows relatively lower sensitivity.
Anti-Bacterial Agents ; Cephalothin ; Chloramphenicol ; Coagulase ; Conjunctivitis* ; Hospitals, General ; Humans ; Incidence ; Infant ; Infant, Newborn ; Outpatients ; Staphylococcus ; Staphylococcus aureus ; Streptococcus

PURPOSE: We studied the changes in the antibiotic sensitivity to the causative organisms of urinary tract infection, between 1979 and 2001, in order to provide useful information on the choice of adequate drugs in the treatment of urinary tract infection (UTI). MATERIALS AND METHODS: We retrospectively analysed 1,370 uropathogens, and their antimicrobial sensitivities, in 647 patients admitted to, or visiting our hospital, between January 1979 and December 2001, that had more than 105cfu/ml in urine culture. RESULTS: The incidence of UTI increased with age, and was highest in the 6th decades (21.0%). Mixed infections increased from 6.1%, in 1979, to 18.4%, by 2001. The common pathogens were E. coli (37.8%), enterococcus (15.0%), Pseudomonas (10.1%) and Klebsiella (9.1%). E. coli was the most important uropathogen during the stated time period. However, the incidence of Gram positive organisms increased from 14.2%, in 1979, to 26.0%, by 2001. In the Gram negative stained uropathogens, the antibiotic sensitivity was changed: ampicillin (11.8 to 14.6%), cephalothin (40.6 to 46.3%) and amikacin (80.5 to 74.8%). For E. coli, trimethoprim/Sulfamethoxazole (TMP/SMX) and ampicillin showed decreased sensitivities of 37.3 and 18.5%, respectively. However, sulbactam/cefoperazone, cefepime and imipenem showed high sensitivities of 96.1, 97.5 and 100%, respectively. CONCLUSIONS: Prior to receiving the bacteriological report, the use of TMP/SMX and ampicillin, as the first choices of treatment for UTI, should be reconsidered. Our results implied that amikacin, which showed the best effects, and was cheaper than fluoroquinolones, can be used as an alternative to these drugs as a primary empirical antibiotic for UTI.
Amikacin ; Ampicillin ; Cephalothin ; Coinfection ; Enterococcus ; Fluoroquinolones ; Humans ; Imipenem ; Incidence ; Klebsiella ; Pseudomonas ; Retrospective Studies ; Urinary Tract Infections* ; Urinary Tract*

The most proper antibiotic must be selected after antimicrobial susceptibility test. If difference in antimicrobial susceptibility was significant between oral sampling sites, it is rationale to use the most susceptible antibiotic agent respond to dental procedure and object of treatment. This study examined sampling site variation from saliva, supragingival plaque and subgingival plaque of 16 children's oral microbes. The cultured bacterial isolates, which were Streptococcus viridans and Neisseria, were examined for 10 antimicribial drugs with the Bauer-Kirby agar disk diffusion method. The used drugs were Penicillin, Ampicillin, Oxacillin, Cephalothin, Imipenem, Gentamicin, Erythromycin, Vancomycin, Ciprofloxacin, Clindamycin. There was no significant difference between three sampling sites for antimicrobial susceptibility test of S. viridans and Neisseria and the sequence of susceptibility was agreed among them. In conclusion, it was suggested that antimicrobial susceptibility test from saliva, supragingival plaque and subgingival plaque of children have no significant sampling site variation.
Agar ; Ampicillin ; Cephalothin ; Child* ; Ciprofloxacin ; Clindamycin ; Diffusion ; Erythromycin ; Gentamicins ; Humans ; Imipenem ; Neisseria ; Oxacillin ; Penicillins ; Saliva ; Vancomycin ; Viridans Streptococci

BACKGROUND: The overuse and misuse of antimicrobial agents and their resultant emergence of resistant microorganisms have made choices regarding antimicrobial therapy more difficult .Therefore,a new system that separated prescribing and dispensing medicine began on July 1,1997 to prevent overuse and misuse of medicine.We studied to evaluate changes of antibiotic sensitivities to causative microorganisms of urinary tract infection 2 years before and after the new medical system. METHODS: During each 2 years before and after the new medical system,we analyzed antibiotic sensitivities of causative microorganisms for urinary tract infection among the 447 out-patients who visited a hospital in GangNeung.The diagnosis of urinary tract infection was based on greater than 105 CFU (Colony For Unit)per ml.urine. RESULTS: The most common pathogenic microorganisms as E.coli (76.5%),followed by Klebsiella pneumoniae (5.1%),and Proteus mirabilis (3.6%)in urine culture. A first generation cephalosporin,cephalothin,against E.coli, had more significant sensitivity after the introduction of the new medical system (52.6%)than before (33.9%),especially in the 80th decade (P=0.023) and in females (P<0.001).Also,trimethoprim/sulfamethoxazole against E.coli showed signifcant improving sensitivity (P=0.025). CONCLUSION: There was little change of antibiotic sensitivity of urinary tract infection in out-patients before and after the new medical system in cephalothin and trimethoprim/sulfamethoxazole against E.coli. The change of antibiotic sensitivities will require further observation for a longer term after the introduction of the new medical system.
Anti-Infective Agents ; Cephalothin ; Diagnosis ; Female ; Humans ; Klebsiella pneumoniae ; Outpatients* ; Prescriptions* ; Proteus mirabilis ; Urinary Tract Infections* ; Urinary Tract*

We evaluated the distribution of normal conjuctival flora and the change of antibiotic sensitivity of cultured bacteria. Specimens were obtained from inferior conjunctiva cul de sac with sterile cotton-tipped applicator from healthy 216 human beings. Antibiotic sensitivity test was done by disk diffusion method. The results indicated that overall positive culture rate was 47.7% and major organisms were Coagulase-negative Staphylococcus(55.7%), Staphylococcus aureus(16%), Gram negative bacteria(7.5%), Streptococcus(4.7%). Antibiotic sensitivity test of cultured Gram positive bacteria demonstrated less than 60% rate of susceptability in gentamicin, tetracyclin, erythromycin and 80.5% rate of susceptability in ciprofloxacin. Gram negative bacteria demonstrated less than 60% rate of susceptability in cephalothin, ampicillin. From the above results, we concluded that antibiotic resistance of normal conjunctival flora was increased from the past and the principle of antiviotic eye drop use should be emphasized.
Ampicillin ; Bacteria ; Cephalothin ; Ciprofloxacin ; Conjunctiva ; Diffusion ; Drug Resistance, Microbial ; Erythromycin ; Gentamicins ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Humans ; Staphylococcus