Search Results

OBJECTIVETo observe the effect of leukemic cells on blood-brain barrier (BBB) in mice with central nervous system leukemia (CNSL) by establishing mice CNSL model and an in vitro BBB model and explore the mechanism of leukemic cell infiltrating central nervous system (CNS).

METHODSAfter splenectomy, cytoxan intraperitoneal injection, and sublethal irradiation, 10 BALB/c nu/nu mice were transplanted intravenously with 1.2 × 10(7) of SHI-1 human monocytic leukemic cells. Mice were monitored for survival and clinical manifestation of nerve palsy. The leukemic cells engrafted were examined by RT-PCR, histopathology and bone marrow (BM) smears. Immunofluorescence analysis with laser scanning fluorescence confocal microscopy was used to determine the expression of fibrinogen and tight-junction protein ZO-1. An in vitro BBB model composed of human brain microvascular endothelial cells (BMVECs) was developed on a Matrigel-based insert. Different leukemic cell lines were seeded onto the upper compartment of transwell insert. After incubated for 24 h with BMVECs, cells that had migrated into the lower compartment were counted and analyzed.

RESULTS(1) Paralysis with or without sight loss was developed in half the mice 30-35 d after innoculated with SHI-1 cells. Leukemic cells infiltrates were observed in BM and in different part of brain tissues including brain parenchyma. The transcriptions of human MLL/AF6 fusion gene were also detected in BM and brain tissues in paralysis mice. The fibrinogen expression and ZO-1 disruption were detected in the infiltrated tissue. (2) After 24 h incubation with leukemic cells, the BMVECs sheets were disrupted and grew singly and ZO-1 expression was down-regulated markedly. SHI-1 cells showed more injurious to BMVECs and higher invasive rate \[(40.33 ± 1.53)% vs (11.83 ± 1.44)%, P < 0.05\] than HL-60 cells did.

CONCLUSIONOne of the mechanisms of leukemic cells infiltrates CNS in CNSL is injure to the BBB.

Animals ; Blood-Brain Barrier ; physiology ; Central Nervous System ; pathology ; Central Nervous System Neoplasms ; pathology ; HL-60 Cells ; Humans ; Leukemia ; pathology ; Mice ; Mice, Inbred BALB C ; Mice, Nude

1.Primary embryonal rhabdomyosarcoma of central nervous system: report of a case.

2.The introduction of glioneuronal and neuronal tumors in 2021 WHO classification of tumors of the central nervous system (5th edition).

3.The introduction of meningiomas in 2021 WHO classification of tumors of the central nervous system (5th edition).

4.The introduction of pediatric-type diffuse high-grade gliomas in 2021 WHO classification of tumors of the central nervous system (5th edition).

5.The present status of diagnosis and treatment of central nervous system metastasis of neuroblastoma.

6.The introduction of embryonal tumors in 2021 WHO classification of tumors of the central nervous system (5th edition).

7.The introduction of pediatric-type diffuse low-grade gliomas in 2021 WHO classification of tumors of the central nervous system (5th edition).

8.Clinicopathological features of primary central nervous system diffuse large B-cell lymphoma presenting with diffuse white matter lesions.

9.WHO classification of tumors of central nervous system (2007): an introduction.

10.Disruption of blood brain-barrier by leukemic cells in central nervous system leukemia.

Display Mode

Output Records

File Type