1.Effects of indium on micronucleus formation in human peripheral blood lymphocytes.
Yan GUO ; Changye HUI ; Liuzhuo ZHANG ; Lili WANG ; Dianpeng WANG ; Xueqin YANG ; Xinyue YANG ; Zhimin LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(8):563-565
OBJECTIVETo investigate the cytotoxicity of indium chloride (InCl₃) and its effects on micro-nucleus formation in primary human lymphocytes cultured in vitro.
METHODSThe CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl₃(4, 40, 80, 200, 500, and 1 000 µmol/L) in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InCl₃and the effects of anti-oxidant vitamin C on micronucleus frequency.
RESULTSLymphocytes exposed to InCl₃of no less than 500 µmol/L had significantly lower survival rates than those in the control group (P < 0.05). Lymphocytes exposed to 80 µmol/L InCl₃had a significantly higher micronucleus frequency than those in the control group (P < 0.05). However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200 µmol/L InCl₃. Lymphocytes cultured in whole blood and exposed to 500 or 1000 µmol/L InCl₃had a significantly increased micronucleus frequency than those in the control group (P < 0.001). The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 µmol/L vitamin C.
CONCLUSIONInCl₃can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.
Cell Nucleus ; metabolism ; Cytokinesis ; DNA Damage ; Humans ; In Vitro Techniques ; Indium ; toxicity ; Lymphocytes ; drug effects ; Oxidative Stress
2.Transient exposure to hydrogen peroxide inhibits the ubiquitination of phosphorylated IkappaBalpha in TNFalpha-stimulated HEK293 cells.
Yeji LEE ; Jin CHOI ; Kyung Ho HA ; Dae Myung JUE
Experimental & Molecular Medicine 2012;44(8):513-520
During ischemia-reperfusion injury, brief pre-exposure to oxidative stress renders organs resistant to subsequent severe damage. NF-kappaB is a transcription factor that is involved in reperfusion-induced inflammatory and immune responses. The activity of NF-kappaB has been shown to be modulated by oxidative stress in various cell types through different pathways. We studied the effect of pre-exposure to oxidative stress on subsequent NF-kappaB activation in TNFalpha-stimulated HEK293 cells. The cells were transiently exposed to 0.5 mM H2O2 for 20 min, prior to stimulation with TNFalpha, and the subsequent expression of NF-kappaB-dependent genes and the levels of NF-kappaB signaling molecules were measured. Pre-exposure to H2O2 significantly delayed the TNFalpha-induced expression of an NF-kappaB reporter gene and inflammatory proteins (intercellular adhesion molecule-1 and IL-1beta). The degradation of inhibitor of NF-kappaB alpha (IkappaBalpha) and the nuclear translocation of NF-kappaB were also delayed by H2O2 treatment, whereas IkappaBalpha phosphorylation and IkappaB kinase activity were not changed. When we examined the ubiquitin/proteosome pathway in H2O2-treated cells, we could not detect significant changes in proteosomal peptidase activities, but we were able to detect a delay of IkappaBalpha poly-ubiquitination. Our results suggest that transient exposure to oxidative stress temporally inhibits NF-kappaB-dependent gene expression by suppressing the poly-ubiquitination of phosphorylated IkappaBalpha in HEK293 cells.
Active Transport, Cell Nucleus
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Cell Nucleus/metabolism
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Enzyme Activation/drug effects
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HEK293 Cells
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Humans
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Hydrogen Peroxide/*pharmacology
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I-kappa B Kinase/antagonists & inhibitors/*metabolism
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Phosphorylation/drug effects
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Protein Transport
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Tumor Necrosis Factor-alpha/*pharmacology
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Ubiquitination/*drug effects
3.Effect of total glucosides of paeony on nuclear factor-kappaB activation in rat peritoneal macrophages.
Gang CHEN ; Xiao-Hong DENG ; Li-Xia GUO ; Jian-Hui LIU
China Journal of Chinese Materia Medica 2008;33(6):669-671
OBJECTIVETo study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS)-induced nuclear factor-kappaB (NF-kappaB) activation in macrophages.
METHODRat peritoneal macrophages were pre-treated with TGP for 2 h and stimulated with LPS for 20 min or 0.5 h. Inhibitory kappaBalpha (IkappaBalpha) protein in the cytoplasm and NF-kappaB p65 protein in the nuclear were analyzed by western blot. Further, DNA binding activity of NF-kappaB complex was detected.
RESULTTGP enhanced the amounts of IkappaBalpha protein in the cytoplasm and decreased the amounts of NF-kappaB p65 protein in the nuclear of LPS-induced macrophages. TGP also inhibited the LPS-mediated DNA binding activity of NF-kappaB complex in macrophages.
CONCLUSIONTGP can inhibit LPS-induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex.
Animals ; Cell Nucleus ; drug effects ; metabolism ; Cytoplasm ; drug effects ; metabolism ; DNA ; metabolism ; Dose-Response Relationship, Drug ; Glucosides ; pharmacology ; Macrophages, Peritoneal ; cytology ; drug effects ; metabolism ; NF-kappa B ; metabolism ; Paeonia ; chemistry ; Protein Transport ; drug effects ; Rats ; Transcription Factor RelA ; metabolism
4.Angiotensin IV upregulates the activity of protein phosphatase 1α in Neura-2A cells.
Dan WANG ; Peng XUE ; Xiu Lan CHEN ; Zhen Sheng XIE ; Fu Quan YANG ; Li ZHENG ; Tao XU
Protein & Cell 2013;4(7):520-528
The peptide angiotensin IV (Ang IV) is a derivative of angiotensin II. While insulin regulated amino peptidase (IRAP) has been proposed as a potential receptor for Ang IV, the signalling pathways of Ang IV through IRAP remain elusive. We applied high-resolution mass spectrometry to perform a systemic quantitative phosphoproteome of Neura-2A (N2A) cells treated with and without Ang IV using sta ble-isotope labeling by amino acids in cell culture (SILAC), and identified a reduction in the phosphorylation of a major Ser/Thr protein phosphorylase 1 (PP1) upon Ang IV treatment. In addition, spinophilin (spn), a PP1 regulatory protein that plays important functions in the neural system, was expressed at higher levels. Immunoblotting revealed decreased phosphorylation of p70S6 kinase (p70(S6K)) and the major cell cycle modulator retinoblastoma protein (pRB). These changes are consistent with an observed decrease in cell proliferation. Taken together, our study suggests that Ang IV functions via regulating the activity of PP1.
Angiotensin II
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analogs & derivatives
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pharmacology
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Animals
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Cell Cycle
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drug effects
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Cell Line, Tumor
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Cell Membrane
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drug effects
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metabolism
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Cell Nucleus
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drug effects
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metabolism
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Cell Proliferation
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drug effects
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Humans
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Mice
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Microfilament Proteins
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metabolism
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Nerve Tissue Proteins
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metabolism
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Neurons
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cytology
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Phosphorylation
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drug effects
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Protein Phosphatase 1
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chemistry
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metabolism
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Protein Transport
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drug effects
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Proteome
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metabolism
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Rats
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Threonine
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metabolism
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Up-Regulation
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drug effects
5.Carbon monoxide inhibits the nuclear-cytoplasmic translocation of HMGB1 in an in vitro oxidative stress injury model of mouse renal tubular epithelial cells.
Yu JIA ; Lu WANG ; Guang-Yuan ZHAO ; Zhi-Qiang WANG ; Song CHEN ; Gang CHEN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2016;36(6):791-795
Carbon monoxide (CO), as a vital small molecule in signaling pathways, is found to be involved in ischemia-reperfusion injury (IRI) in renal transplantation. CO-releasing molecule-2 (CORM-2), a CO-releasing molecule, is a type of metal carbonyl complexes which can quickly release CO in vivo. In this study, an in vitro oxidative stress injury model was established to examine the effect of CORM-2 pretreatment on the nuclear-cytoplasmic translocation of high mobility group box 1 protein (HMGB1) in mouse primary renal proximal tubular epithelial cells (RPTECs). Immunofluorescence staining showed that HMGB1 in the medium- and CORM-2-treated groups was predominantly localized in the nucleus of the cells, whereas higher amounts of HMGB1 translocated to the cytoplasm in the HO- and inactive CORM-2 (iCORM-2)-treated groups. Western blotting of HMGB1 showed that the total amounts of cytoplasmic HMGB1 in the HO-treated (0.59±0.27) and iCORM-2-treated (0.57±0.22) groups were markedly higher than those in the medium-treated (0.19±0.05) and CORM-2-treated (0.21±0.10) groups (P<0.05). Co-immunoprecipitation showed that the levels of acetylated HMGB1 in the HO-treated (642.98±57.25) and iCORM-2-treated (342.11±131.25) groups were markedly increased as compared with the medium-treated (78.72±74.17) and CORM-2-treated (71.42±53.35) groups (P<0.05), and no significant difference was observed between the medium-treated and CORM-2-treated groups (P>0.05). In conclusion, our study demonstrated that in the in vitro oxidative stress injury model of primary RPTECs, CORM-2 can significantly inhibit the nuclear-cytoplasmic translocation of HMGB1, which is probably associated with the prevention of HMGB1 acetylation.
Active Transport, Cell Nucleus
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drug effects
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Animals
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Carbon Monoxide
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pharmacology
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Cell Nucleus
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metabolism
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Cells, Cultured
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Epithelial Cells
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drug effects
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metabolism
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HMGB1 Protein
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metabolism
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Kidney Tubules
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cytology
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Mice
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Organometallic Compounds
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pharmacology
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Oxidative Stress
6.Synergistic Effect of Interleukin-6 and Hyaluronic Acid on Cell Migration and ERK Activation in Human Keratinocytes.
Jee Hyun CHOI ; Jin Hyun JUN ; Ji Hyun KIM ; Ho Joong SUNG ; Jong Hun LEE
Journal of Korean Medical Science 2014;29(Suppl 3):S210-S216
Wound healing is initiated and progressed by complex integrated process of cellular, physiologic, and biochemical events, such as inflammation, cell migration and proliferation. Interleukin 6 (IL-6) is a multifunctional cytokine, and it could regulate the inflammatory response of wound healing process in a timely manner. Hyaluronic acid (HA) is an essential component of the extracellular matrix, and contributes significantly to cell proliferation and migration. The purpose of this study was to investigate the effects of IL-6 or/and HA on the cell migration process in human keratinocytes. Combining IL-6 and HA significantly increased the cell migration in scratch based wound healing assay. The phosphorylation of extracellular-signal-regulated kinase (ERK) was significantly increased after 1 hr of IL-6 and HA treatment, but the phosphorylation of p38 mitogen-activated protein kinase (MAPK) was not. We also found that significant increase of the NF-kappaB translocation from cytoplasm into nucleus after 1 hr of IL-6 or/and HA treatments. This study firstly showed that synergistic effects of combining IL-6 and HA on the cell migration of wound healing by activation of ERK and NF-kappaB signaling. Further studies might be required to confirm the synergistic effects of HA and IL-6 in the animal model for the development of a novel therapeutic mixture for stimulation of wound healing process.
Active Transport, Cell Nucleus/drug effects
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Cell Line
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Cell Movement/*drug effects
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Cell Proliferation/drug effects
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Cell Survival/drug effects
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Enzyme Activation/drug effects
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Extracellular Signal-Regulated MAP Kinases/*metabolism
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Humans
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Hyaluronic Acid/*pharmacology
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Interleukin-6/*pharmacology
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Keratinocytes/*metabolism
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MAP Kinase Signaling System/drug effects
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NF-kappa B/metabolism
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Phosphorylation/drug effects
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Protein Transport/drug effects
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Wound Healing
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p38 Mitogen-Activated Protein Kinases/metabolism
7.Observation of penetration, distribution and accumulation in human renal proximal tubular epithelial cells by aristololactam-I.
Pu SHANG ; Xuan WANG ; Xiao-Mei LI ; Jia-Wei TANG ; Shao-Qing CAI
China Journal of Chinese Materia Medica 2008;33(7):793-797
OBJECTIVETo study whether aristololactam I (AL-I) can enter renal proximal tubular epithelial cells and the situation of intracellular distribution and accumulation.
METHODCultured human renal proximal tubular epithelial cell line (HK-2) was used as the subject. Intracellular fluorescence from AL-I and its distribution are examined by fluorescence microscopy after a treatment with different concentration of AL-I, the intracellular accumulation of AL-I was also investigated by incubated cells in AL-I -free medium for 48 h after washing-out the media containing AL-I.
RESULTAfter treatment of AL-I (concentration from 5 microg x mL(-1) to 20 microg x mL(-1)), glaucous fluorescence could be observed inside renal proximal tubular epithelial cells at 0.5 h, and the fluorescence distributed only in cytoplasm while not be observed in nuclei. Moreover, the fluorescence of AL-I could be kept in cytoplasm for more than 48 h after washing out the media containing AL-I .
CONCLUSIONAL-I is able to enter renal proximal tubular epithelial cells in short time and accumulate in cytoplasm, but not enter nuclei. This property may contribute to the cytotoxic mechanism of renal injury induced by AL-I, which may partially explain the persistent renal toxicity of AAs and its metabolites in the development of aristolochic acid nephropathy.
Animals ; Aristolochic Acids ; metabolism ; toxicity ; Cell Line ; Cell Nucleus ; drug effects ; metabolism ; Cytoplasm ; drug effects ; metabolism ; Epithelial Cells ; cytology ; drug effects ; metabolism ; pathology ; Humans ; Kidney Diseases ; metabolism ; pathology ; Kidney Tubules, Proximal ; cytology ; pathology ; Microscopy, Fluorescence
8.The effect of silicon dioxide on the activation of nuclear factor-kappaB in THP-1 cells.
Jian-ya ZHOU ; Guo-gen MAO ; Fa-di TANG ; Jian-ying ZHOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):179-181
OBJECTIVETo study the effect of silicon dioxide (SiO(2)) on the activation of nuclear factor-kappaB (NF-kappaB) in THP-1 cell line.
METHODSTHP-1 cells were incubated with a series of doses of SiO(2) (0, 100, 200 micro g/ml). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-kappaB/p65 in nuclei was measured by Western blot analysis.
RESULTSThe majority of fluorescein isothiocyanate (FITC)-labelled NF-kappaB/p65 located in the nuclei 30 min after stimulation by 100 micro g/ml SiO(2), whereas the FITC-labelled NF-kappaB/p65 were mainly seen in the plasma of normal control cells. The expression of NF-kappaB/p65 in THP-1 nuclear protein was low in control group (0 micro g/ml SiO(2)) while it increased after stimulation by 100 micro g/ml SiO(2) and 200 micro g/ml SiO(2) for 15 min and 30 min. The level of NF-kappaB/p65 was comparatively increased with the increasing of doses and time. Lipopolysaccharides (LPS), an activator of NF-kappaB, had similar effect as SiO(2) on the activation of NF-kappaB/p65 in THP-1 cells.
CONCLUSIONSiO(2) could activate and internalize NF-kappaB in the THP-1 cell line.
Blotting, Western ; Cell Line ; Cell Nucleus ; drug effects ; metabolism ; Dose-Response Relationship, Drug ; Fluorescent Antibody Technique, Indirect ; Humans ; Microscopy, Confocal ; NF-kappa B ; metabolism ; Silicon Dioxide ; pharmacology
9.The RNA Pol II sub-complex hsRpb4/7 is required for viability of multiple human cell lines.
Yang ZHAO ; Kim K C LI ; King Pan NG ; Chi Ho NG ; Kevin A W LEE
Protein & Cell 2012;3(11):846-854
The evolutionarily conserved RNA Polymerase II Rpb4/7 sub-complex has been thoroughly studied in yeast and impacts gene expression at multiple levels including transcription, mRNA processing and decay. In addition Rpb4/7 exerts differential effects on gene expression in yeast and Rpb4 is not obligatory for yeast (S. cerevisiae) survival. Specialised roles for human (hs) Rpb4/7 have not been extensively described and we have probed this question by depleting hsRpb4/7 in established human cell lines using RNA interference. We find that Rpb4/7 protein levels are inter-dependent and accordingly, the functional effects of depleting either protein are co-incident. hsRpb4/7 exhibits gene-specific effects and cells initially remain viable upon hsRpb4/7 depletion. However prolonged hsRpb4/7 depletion is cytotoxic in the range of cell lines tested. Protracted cell death occurs by an unknown mechanism and in some cases is accompanied by a pronounced elongated cell morphology. In conclusion we provide evidence for a gene-specific role of hsRpb4/7 in human cell viability.
Cell Line
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Cell Nucleus
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metabolism
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Cell Survival
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drug effects
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Gene Expression Profiling
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HeLa Cells
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Humans
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RNA Interference
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RNA Polymerase II
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antagonists & inhibitors
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genetics
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metabolism
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RNA, Small Interfering
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pharmacology
10.Activation of nucleus-independent signals triggered by N-methyl-N'-nitro-N- nitrosoguanidine.
Zheng WANG ; Gu-liang WANG ; Jun YANG ; Zhi-hua GAO ; Ying-nian YU
Journal of Zhejiang University. Medical sciences 2003;32(5):385-389
OBJECTIVETo study the effect of MNNG on inducement of non-targeted mutation and activation of several cellular signal transduction pathways, and to determine whether the activation of these signaling pathways was dependent on the DNA-damage.
METHODSVero cells were enucleated by discontinuous density centrifugation. The PKA activities were measured by enzyme-linked immunosorbent assay. The status of cell membrane receptors was studied with immunofluorescent staining and confocal microscopy.
RESULTIn enucleated cytoplasts, MNNG-treatment increased PKA activity for about 2.3-fold in accordance with the 2.7-fold up-regulation of PKA activity in whole vero cells exposed to MNNG. The clustering of cell surface receptors of epidermal growth factor and tumor necrosis factor alpha was also observed in cells exposed to MNNG; this phenomenon was also found in enucleated cells.
CONCLUSIONThe results indicate that the initiation of signal cascades induced by low concentration of MNNG might be associated with its interaction with cell surface receptors and/or direct activation of related signal proteins but not its DNA damage.
Animals ; Cell Nucleus ; physiology ; Cercopithecus aethiops ; Cyclic AMP-Dependent Protein Kinases ; metabolism ; DNA Damage ; Enzyme Activation ; drug effects ; Methylnitronitrosoguanidine ; toxicity ; Receptor, Epidermal Growth Factor ; drug effects ; metabolism ; Receptors, Tumor Necrosis Factor ; drug effects ; metabolism ; Signal Transduction ; drug effects ; Vero Cells