Pulsed electromagnetic field (PEMF), a non-invasive physical treatment modality, is now used clinically to promote bone formation for osteoporosis. The patients after ectomy of ovarian cancer are easily complicated with osteoporosis. However, the safety parameters of PEMF treatment for the osteoporosis patients after resection of ovarian cancer remain unknown. Therefore, this study was designed to examine the effect of different frequency of PEMF on the proliferation, apoptosis and migration of human ovarian cancer cells (SKOV3 cells). Cultured SKOV3 cells were exposed to PEMF stimulation daily with radiation of 8 Hz, 16 Hz, 32 Hz and 64 Hz, respectively. We used sinusoidal waves with strength of 1 mT, twice a day with an interval of 12 hours. An exposure to the waves lasted 30 minutes, for 3 days, with those no PEMF stimulation serving as the control. The proliferation of cells was detected using EdU assay, and the apoptosis of cell was assessed with Annexin V-FITC fluorescence. The migration of cells was measured with the scratch wound assay. The data showed that exposure to PEMF of 1 mT, 8 Hz for 3 days could significantly inhibit the proliferation of SKOV3 cells and induce the apoptosis of the cells. The migrated distance and number were increased by 1 mT, 8 Hz or 32 Hz PEMF stimulation, but decreased by 1 mT, 16 Hz treatment. The results suggested that we should be careful about the safety of PEMF treatment and strictly choose the optical parameters in preventing or treating the osteoporosis of the patients after resection of ovarian cancer.
Apoptosis ; radiation effects ; Cell Line, Tumor ; Cell Movement ; radiation effects ; Cell Proliferation ; radiation effects ; Cystadenocarcinoma, Serous ; pathology ; Electromagnetic Fields ; adverse effects ; Female ; Humans ; Ovarian Neoplasms ; pathology

OBJECTIVEInvestigations were carried out to understand the effect of 50 Hz power frequency magnetic field on microfilament assembly of human amniotic cells and on expression of actin and epidermal growth factor receptor.

METHODSHuman amnion FL cells were exposed to 0.1, 0.2, 0.3, 0.4, 0.5 mT power frequency magnetic field for 30 minutes. Microfilaments were marked using Phalloidin-TRITC, and then were observed under a fluorescence microscope. An optical method was used to detect the relative content of microfilament in cells. A scanning electron microscope was used to detect the cell shape. The content of actin and epidermal growth factor receptor in the preparation of the detergent-insoluble cytoskeleton were measured by western-blotting to analyse the potential mechanism of the change induced by magnetic field.

RESULTSIntracellular stress fibers were found to decrease after exposing cells to a 0.2 mT power frequency magnetic field for 30 minutes. New microfilament and filopodia bundles appeared at the cell periphery after exposure, but the detected total F-actin content per cell was not significantly changed, detected by a F-actin-specific dye. The change in the amount of microfilaments caused by the field could be recovered 2 hours later when the field was withdrawn. The mean height of microfilament cytoskeleton decreased from (12.37 +/- 1.28) microm to (9.97 +/- 0.38) microm (t = 6.96, P > 0.05) after exposure using a confocal microscope. The cell shapes became more flat and lamellipodia appeared after exposure observed by a scanning electron microscope. By using Western blotting method, the intracellular contents of epidermal growth factor receptor and of actin in the preparation of the detergent-insoluble cytoskeleton which are associated with high-affinity epidermal growth factor receptors, increased about (31.2 +/- 4.1)% (t = 17.10, P < 0.05) and (16.8 +/- 2.3)% (t = 16.68, P < 0.05) respectively, compared with that of the control.

CONCLUSIONThese results suggest that a short time exposure to a 0.2 mT power frequency magnetic field induces re-organization of microfilament in human amnion FL cells. These changes could be recovered by field withdraw and may have something with the clustering of epidermal growth factor receptors induced by magnetic field.

Actin Cytoskeleton ; metabolism ; Amnion ; cytology ; radiation effects ; Cell Line ; Cell Movement ; Cytoskeleton ; metabolism ; radiation effects ; Electromagnetic Fields ; Humans ; Receptor, Epidermal Growth Factor ; metabolism ; Signal Transduction

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.
Blood Platelets/*cytology/*metabolism ; Cell Movement/radiation effects ; Cell Proliferation/radiation effects ; Cells, Cultured ; Collagen/metabolism ; Fibrin/*metabolism ; Fibroblasts/*cytology/metabolism/*radiation effects ; Humans ; Skin/*cytology ; Time Factors ; Ultraviolet Rays/*adverse effects

OBJECTIVETo investigate the feasibility of bone marrow mesenchymal stem cell (MSC) transplantation with ultrasound-targeted microbubble destruction.

METHODSTwenty-one Wistar rats were divided into MSCs-iv group (MSCs-iv), ultrasound+MSCs-iv group (US+MSCs-iv), ultrasound+microbubble+MSCs-iv group (US+MB+MSCs-iv) with intravenous MSC transfer, ultrasound and microbubble treatment as indicated. The skeletal muscles were obtained from the rats for microscopic examination with HE staining. The hindlimb gracilis and semimembranosus muscles were sampled 7 days after MSC transplantation, and the transplanted MSCs were detected by immunohistochemistry. The vital organs were collected from rats in US+MB+MSCs-iv group for immunohistochemistry.

RESULTSIn US+MB+MSCs-iv group, HE staining demonstrated the presence of red blood cell leakage into the tissue space in the gracilis and semimembranosus muscles, and immunohistochemistry identified large numbers of transplanted MSCs in the the gracilis and semimembranosus muscles and the spleen, whereas no labeled cells were detected in the skeletal muscles in other groups.

CONCLUSIONUltrasound-targeted microbubble destruction provides a useful means for enhancing the efficiency of stem cell transplantation.

Animals ; Bone Marrow Cells ; cytology ; Cell Movement ; radiation effects ; Female ; Male ; Mesenchymal Stem Cell Transplantation ; methods ; Mesenchymal Stromal Cells ; cytology ; Microbubbles ; Muscle, Skeletal ; cytology ; Rats ; Rats, Wistar ; Ultrasonics

Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme (GBM). The purpose of this study was to determine the effect of radiation on the growth characteristics of primary human GBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues were implanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonance imaging on day 77 (baseline) after implantation. The rats underwent irradiation to a dose of 50 Gy delivered subcuratively on day 84 postimplantation (n = 8), or underwent no radiation (n = 8). Brain tissues were obtained on day 112 (nonirradiated) or day 133 (irradiated). Immunohistochemistry was performed to determine tumor cell proliferation (Ki-67) and to assess the expression of infiltration marker (matrix metalloproteinase-2, MMP-2) and cell migration marker (CD44). Tumor neovascularization was assessed by microvessel density using von-Willebrand factor (vWF) staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11 weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoing radiation was (71 +/- 15)% compared with (25 +/- 12)% in the nonirradiated group (P = 0.02). The number of MMP-2-positive areas and proportion of CD44-positive cells were also high in tumors receiving radiation, indicating great invasion and infiltration. Microvessel density analysis did not show a significant difference between nonirradiated and irradiated tumors. Taken together, we found that subcurative radiation significantly increased proliferation, invasion, and migration of primary GBM. Our study provides insights into possible mechanisms of treatment resistance following radiation therapy for GBM.
Animals ; Brain Neoplasms ; metabolism ; pathology ; radiotherapy ; Cell Line, Tumor ; Cell Movement ; radiation effects ; Cell Proliferation ; radiation effects ; Female ; Glioblastoma ; metabolism ; pathology ; radiotherapy ; Humans ; Hyaluronan Receptors ; metabolism ; Immunohistochemistry ; Ki-67 Antigen ; metabolism ; Magnetic Resonance Imaging ; Matrix Metalloproteinase 2 ; metabolism ; Microvessels ; pathology ; Neoplasm Transplantation ; Neovascularization, Pathologic ; pathology ; Radiation Tolerance ; Radiotherapy, High-Energy ; Rats ; Rats, Nude