No abstract available.
Cell Culture Techniques*

No abstract available.
Cell Culture Techniques*

Background: In recent years, the rotavirus diarrhea increased significantly in Vietnam, an estimated 50%-70% of children\u2019s hospitalization for diarrhea in 2005. The virus subtype causing disease mainly in Vietnam today was not only G1, G2, G4, like other countries in the world but also the rare strain was G9. Objectives: to isolate G9 rotavirus strains on MA104 cell culture; to selecting G9 rotavirus which developing well on MA104 cells. Subjectives and Method: an experimental research in the laboratory. 20 stool samples derived from 20 children with acute diarrhea caused by rotavirus G9P8 (using RT-PCR method). The samples were processed according to standards of the Centers for disease control and prevention (CDC), Atlanta, USA. Results: eight out of 20 human rotavirus strains G9P8 were positive on Ma104 cell suspension after 3 consecutive cultures (OD indexes of these sample were over 0.100,sample No 2 had the highest OD (1.347)). Sample No 2 was chosen for the first time cloning (25 clones, 17/25 with OD>0.100). And clone No 16 was selected for the second purifying (25 clones, 24/25 with OD>0.100). Ten out of 25 clones in the second time were adapted on monolayer Ma104 cell culture and only clone No 14 and No 18 with highest OD (2.648 and 2.644, respectively) will be used for next studies. Conclusions: cloning method was a basic method for adapting rotavirus clone on cell cultured. Rate of G9P8 rotavirus strain which isolated on Ma104 cells\ufffd?suspension was relatively high. Among the second time clones, 2/10 samples adapted on Ma104 cells with high OD.
Cell Culture Techniques ; Rotavirus ;

Using human rotavirus strain VNHR203-027 to inoculate and passage 11 consecutive times on Africa green monkey kidney (Vero) cells with inoculum medium at different trypsine concentrations, their results are VNHR203-027 have been adapting and growing on Vero cells with trypsin concentration of 30g/ml. Using human rotavirus strain VNHR203-037 to multiply on 4 lots of primary monkey kidney cells and 6 lots of Vero cells gave very high titre on both kinds of cell, the average titre on primary monkey kidney cells was 8,47 Iog10 FFU/ml and of Vero cells was 8.65 Iog10 FFU/ml. No significant difference in potency is found among Vero cells and primary monkey kidney cells (T=10-2 < t=1.86 with =0.05).
Rotavirus ; Cell Culture Techniques

Statement of problem. The success of osseointegration can be enhanced with an implant that has improved surface characteristics. Anodic oxidation is one of the surface modifying method to achieve osseointegration. Voltage of anodic oxidation can change surface characteristics and cell activity. Purpose. This study was performed to evaluate MG63 cell responses such as affinity, proliferation and to compare surface characteristics of anodic oxidized titanium in various voltage. Material and method. The disks for cell culture were fabricated from grade 3 commercially pure titanium, 1 mm in thickness and 12 mm in diameter. Surfaces of 4 different roughness were prepared. Group 1 had a machined surface, used as control. Group 2 was anodized under 220 V, group 3 was anodized under 300 V and group 4 was anodized under 320 V. The microtopography of specimens was observed by scanning electron microscope (JSM-840A, JEOL, Japan) and atomic force microscope(Autoprobe CP, Park Scientific Instrument, USA). The surface roughness was measured by confocal laser scanning microscope(Pascal, LSM5, Zeiss, Germany). The crystal structure of the titanium surface was analyzed with x-ray diffractometer(D8 advanced, Bruker, Germany). MG63 osteoblast-like cells were cultured on these specimens. The cell morpholgy was observed by field emission electron microscope(Hitachi S-4700, Japan). The cell metabolic and proliferative activity was evaluated by MTT assay. Results and conclusion. With in limitations of this in vitro study, the following conclusions were drawn. 1. In anodizing titanium surface, we could see pores which did not show in contol group. In higher anodizing voltage, pore size was increased. 2. In anodizing titanium surface, we could see anatase. In higher anodizing voltage, thicker oxide layer increased crystallinity(anatase, anatase and rutile mixed). 3. MG63 cells showed more irregular, polarized and polygonal shape and developed more lamellipodi in anodizing group as voltage increased. 4. The activity of cells in MTT assay increased significantly in group 3 and 4 in comparison with group 1 and 2. However, there was no difference between group 3 and 4 at P<0.05. Proliferation of MG63 cells increased significantly in pore size(3-5.5 micrometer) of group 3 and 4 in comparison with in pore size(0.2-1 micrometer) of group 2.
Cell Culture Techniques ; Osseointegration ; Titanium*

A single B17.3 strain was selected from 45 Rotavirus strains by the method of CDC-Atlanta, USA - cloning and adaptation assay on cell culture. The strain is one of seed candidates for Rota vaccine production in Vietnam.
Rotavirus ; Cells ; Cell Culture Techniques

Rotavirus strains isolated from 3 acute diarrhea calves at Ba Vi cattle farm were cultured and firstly cloned in African green monkey kidney (Vero) cells and African Rhesus monkey kidney (MA104) cells. After 3 times of passage, the calf rotavirus strains were secondly cloned and all of them were adapted in primary kidney cells of Macaca mulatta
Rotavirus ; Cell Culture Techniques ; Diarrhea

Different kinds of forces can be applied to the biological tissue. The analysis of the applied force is highly important to explain the mechanism of cellular response. In this study, the applied force to the collagen gel was analyzed by the finite elements analysis. The model received two different kinds of static force (compression and tension). The force range was 50g to 400g. In results, von Mises stress was concentrated in the peripheral region in the compression model. It was concentrated in the central area in the tension model. However, the compressive force was high in the peripheral area of the compression model and the tensional force was also high in the same area of the tension model. In conclusion, the applied force could be different to the region and it should be considered in the experiment to analyze the effects of the mechanical force on the cells.
Cell Culture Techniques ; Collagen ; Finite Element Analysis

Different kinds of forces can be applied to the biological tissue. The analysis of the applied force is highly important to explain the mechanism of cellular response. In this study, the applied force to the collagen gel was analyzed by the finite elements analysis. The model received two different kinds of static force (compression and tension). The force range was 50g to 400g. In results, von Mises stress was concentrated in the peripheral region in the compression model. It was concentrated in the central area in the tension model. However, the compressive force was high in the peripheral area of the compression model and the tensional force was also high in the same area of the tension model. In conclusion, the applied force could be different to the region and it should be considered in the experiment to analyze the effects of the mechanical force on the cells.
Cell Culture Techniques ; Collagen ; Finite Element Analysis

Background: \r\n', u'Rotavirus is the major important cause of infectious infantile gastroenteritis and diarrhea, especially the children aged 6 months to 24 months. Some efforts made to provide safe water and cultivate the hygiene have not reduced the diarrhea caused by rotaviruses. Develope a vaccine agaisnt this virus is of high importance. A question about the production of this kind of vaccine targeted athow to make human rotaviruses strains adapt cultured cells.\r\n', u'Objectives\r\n', u'To determine the multiplicity of infection (MOI) of human rotaviruses strains in vero cell.\r\n', u'Subjects and method: \r\n', u'The multiplicity of infection of Human rotaviruses strains (G1 P8, G1P4, and G4P6) were selected and the CDC- Atlanta US supplied the vero cell.Use vero cells cultivate, temper the vero cell and, applying direct immunofluorescence to determine the multiplicity of G1P8, G1P4, C4P6.\r\n', u'Results:\r\n', u'The MOI of G1P8, G1P4 and C4P6 are 0.02 ffu/cell, 0.15ffu/cell, and 0.35 ffu/cell, respectively.\r\n', u'Conclusion:\r\n', u'Using the vero cell to multiple the rotaviruses is the effective part during the production of vaccines against rotaviruses. The MOI of human rotaviruses strains G1P8, G1P4 and G4P6 are in order:0,02 ffu/cell, 0,15ffu/cell, and 0,35% ffu/cell. \r\n', u'
Rotavirus ; Vero Cells ; Cell Culture Techniques ;