2.Ability of catechin to eliminate O2-* and *OH.
Xiao-jie HE ; Zhu-wen YI ; Yun TIAN ; Xiang-yang LU ; Xi-qiang DANG ; Shuang-hong MO ; Hua-bing YANG
Journal of Central South University(Medical Sciences) 2006;31(1):138-140
OBJECTIVE:
To investigate the eliminating ability of catechin to eliminate O2-* and *OH.
METHODS:
The ability of catechin to clear away O2-* and *OH was respectively measured by faintness chemiluminescence and spin trapping assay.
RESULTS:
IC50 that catechin eliminated O2-* and *OH was 6.16, 0.59 g/mL respectively, and the eliminating ability of catechin was much stronger than that of the extract from liquorice, rosemary, grape pip, giant knotweed and ginkgo leaf.
CONCLUSION
Compared with several important natural plants of antioxidants, the eliminating ability of cathechin is the best.
Antioxidants
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pharmacology
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Catechin
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pharmacology
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Free Radical Scavengers
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pharmacology
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Hydroxyl Radical
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metabolism
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Luminescent Measurements
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Superoxides
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metabolism
3.In Vitro Antifungal Activity of Epigallocatechin 3-O-Gallate against Clinical Isolates of Dermatophytes.
Bong Joo PARK ; Hideaki TAGUCHI ; Katsuhiko KAMEI ; Tetsuhiro MATSUZAWA ; Suong Hyu HYON ; Jong Chul PARK
Yonsei Medical Journal 2011;52(3):535-538
Previously, we reported that epigallocatechin 3-O-gallate (EGCg) has growth-inhibitory effect on clinical isolates of Candida species. In this study, we investigated the antifungal activity of EGCg and antifungal agents against thirty-five of dermatophytes clinically isolated by the international guidelines (M38-A2). All isolates exhibited good susceptibility to EGCg (MIC50, 2-4 microg/mL, MIC90, 4-8 microg/mL, and geometric mean (GM) MICs, 3.36-4 microg/mL) than those of fluconazole (MIC50, 2-16 microg/mL, MIC90, 4-32 microg/mL, and GM MICs, 3.45-25.8 microg/mL) and flucytosin (MIC50, MIC90, and GM MICs, >64 microg/mL), although they were less susceptible to other antifungal agents, such as amphotericin B, itraconazole, and miconazole. These activities of EGCg were approximately 4-fold higher than those of fluconazole, and were 4 to 16-fold higher than flucytosin. This result indicates that EGCg can inhibit pathogenic dermatophyte species. Therefore, we suggest that EGCg may be effectively used solely as a possible agent or combined with other antifungal agents for antifungal therapy in dermatophytosis.
Antifungal Agents/*pharmacology
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Arthrodermataceae/*drug effects/isolation & purification
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Catechin/*analogs & derivatives/pharmacology
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Microbial Sensitivity Tests
4.Advance of chemistry and bioactivities of catechin and its analogues.
China Journal of Chinese Materia Medica 2004;29(10):1017-1021
Catechin and its analogues are varied and distributed widely. They have significant bioactivity on clearing free radical, anti-cancer, anti-flammatory, anti-allergic, anti-mutation, anti-aging, improving liver function, and so on. Recently, catechins (GTC) in green tea have given rise to comprehensive attention all over the world, and will have a wonderful prospect. The progress in studies of the structures, spectrum characters, resources and bioactivities of catechin and its analogues has been reviewed.
Animals
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Antineoplastic Agents, Phytogenic
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pharmacology
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Catechin
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analogs & derivatives
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chemistry
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pharmacology
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Free Radical Scavengers
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pharmacology
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Humans
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Platelet Aggregation Inhibitors
;
pharmacology
5.Stimulating effect of catechin, an active component of Spatholobus suberectus Dunn, on bioactivity of hematopoietic growth factor.
Dong-xiao WANG ; Ping LIU ; Yi-hong CHEN ; Ruo-yun CHEN ; Dai-hong GUO ; Hao-yang REN ; Meng-li CHEN
Chinese Medical Journal 2008;121(8):752-755
BACKGROUNDHematopoietic growth factor (HGF) is indispensable to hematopoiesis in the body. The proliferation and differentiation of hematopoietic cells must rely on the existence and stimulation of HGF. This study investigated the effect of catechin, an active component extracted from Spatholobus suberectus Dunn (SSD), on bioactivity of granulocyte-macrophage colony-stimulating activity (GM-CSA), burst-promoting activity (BPA) and megakaryocyte colony-stimulating activity (MK-CSA) in spleen condition medium (SPCM) of mice to clarify the hematopoietic mechanism of catechin and SSD.
METHODSSpleen cells of mice were separated and spleen condition medium (SPCM) was prepared from spleen cell culture. Bone marrow cells of mice were separated and cultured in a culture system including 10% (v/v) SPCM (induced by catechin in vivo or ex vivo) for 6 days. Granulocyte-macrophage colony forming units (CFU-GM), erythrocyte burst-colony-forming units (BFU-E) and megakaryocyte colony-forming units (CFU-Meg) formation were employed to assay the effects of different treatment on the bioactivity of GM-CSA, BPA and MK-CSA in SPCM.
RESULTSSPCM induced by 100 mg/L catechin ex vivo could promote the growth of CFU-GM, BFU-E and CFU-Meg, which indicated that catechin could stimulate the production of GM-CSA, BPA and MK-CSA in SPCM. SPCM prepared at the fourth day of spleen cell culture showed the best stimulating activity. The bioactivity of GM-CSA, BPA and MK-CSA in the SPCM prepared after intraperitoneally injecting catechin into mice was also increased. The number of CFU-GM, BFU-E and CFU-Meg gradually increased as the dose of catechin increased and the time of administration prolonged. CFU-GM, BFU-E and CFU-Meg of the high-dose catechin group were significantly higher than those of the control group (P < 0.01) and reached the maximum at the seventh day after administration.
CONCLUSIONSThis study suggests that catechin extracted from the active acetic ether part of Spatholobus suberectus Dunn can regulate hematopoiesis by inducing bioactivity of GM-CSA, BPA and MK-CSA in SPCM of mice. This may be one of the mechanisms for the hematopoietic-supportive effect of catechin and Spatholobus suberectus Dunn.
Animals ; Catechin ; pharmacology ; Granulocyte-Macrophage Colony-Stimulating Factor ; physiology ; Hematopoiesis ; drug effects ; Interleukin-3 ; physiology ; Mice ; Thrombopoietin ; physiology
6.Effects of loaded buffer with epigallocatechin gallate on physiological functions of platelets.
Lei FANG ; Jie-Xi WANG ; Min-Xia LIU ; Wei DU ; Su-Ping REN ; Guo-Bo QUAN ; Yan WANG ; Ying HAN
Journal of Experimental Hematology 2011;19(3):764-768
This study was aimed to explore the change of aggregation and activation of platelets loaded with epigallocatechin gallate (EGCG). The platelets were treated by loading buffer with different concentrations of EGCG (0, 2.5, 5, 7.5, 10, 12.5, 15, 20 and 30 mmol/L) and were divided into 2.5, 5, 10 mmol/L groups and control group. The physiological and biochemical functions of platelets were observed, including recovery rate, aggregation and activation of platelets. The platelet counts were determined by Counter Cell-DYN 1200. The aggregation activities were tested through turbidimetry, the platelet apoptosis was detected by flow cytometry. The results showed that the concentrations of EGCG loading in platelets of 2.5, 5 and 10 mmol/L groups were 0.4006 ± 0.12, 1.0527 ± 0.1503, 1.6902 ± 0.1112 mmol/L respectively. Along with the increasing of EGCG concentrations in loading-buffer, the EGCG absorbed by platelets increased too. When the concentration of EGCG in loading-buffer exceeded 15 mmol/L, the EGCG absorbed by platelets did not increase. The recovery rate in 2.5 mmol/L loading buffer group was 82.45 ± 0.360% which was lower than that in control group (90.33 ± 1.115%) (p < 0.05). As compared with control group, the recovery rate in 5 mmol/L loading buffer group (57.51 ± 2.468)% and 10 mmol/L loading buffer group (47.45 ± 2.030)% were even significantly lower (p < 0.01). When ADP was used as the inducer, the maximal aggregation rate (MAR) in control group was (63.6 ± 4.037)%, which was higher than that in other EGCG-loading groups (p < 0.01). And the aggregation activity of platelets negatively correlated with the concentration of EGCG in loading-buffer. When THR was used as the inducer, the MAR in control group was (89.3 ± 6.533)% and higher than that those in other groups (p < 0.05), especially in groups with loading-buffer higher than 10 mmol/L EGCG (70.1 ± 5.400%) (p < 0.01). In the experiment of cellular apoptosis, the early apoptosis easy appeared in platelets loaded with EGCG. It is concluded that the EGCG loading in platelets markedly influences the physiological and biochemical functions of platelets, the apoptosis easy occurs in platelets loaded with EGCG. The EGCG accelerates the course of platelet apoptosis.
Apoptosis
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drug effects
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Blood Platelets
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drug effects
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Catechin
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analogs & derivatives
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pharmacology
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Flow Cytometry
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Humans
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Platelet Aggregation
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drug effects
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Platelet Count
7.Can green tea protect against not only sunburn but also melanoma?
Chinese Medical Journal 2006;119(12):1056-1056
8.Effect of epigallocatechin gallate on lactacystin-induced PC12 cell injury.
Mei-fen DAI ; Dan HU ; Dan ZHAO
Chinese Journal of Integrated Traditional and Western Medicine 2011;31(5):672-675
OBJECTIVETo study the effect of epigallocatechin gallate (EGCG) against lactacystin induced PC12 cell injury.
METHODSThe inoculated rat PC12 cells were cultured for 24 h, followed by intervention. The cells were divided into 5 groups, i.e., the normal control group, 10 micromol/L lactacystin injury group, and the EGCG pretreated groups (at the final concentration of 5, 10, and 50 micromol/L, respectively). The cytoactive was detected by MTT colorimetry. Morphological changes of the cell nucleus were observed by Hoechst 33,258 staining, and the apoptosis ratio was detected by flow cytometry (FCM).
RESULTSEGCG at different doses showed protective effect on lactacystin-induced PC12 cell injury. Compared with the lactacystin injury group [(61.22 +/- 1.02)%], the cytoactive in EGCG pretreated groups at the final concentration of 5, 10, and 50 micromol/L, respectively increased obviously to (66.99 +/- 1.30)%, (66.67 +/- 0.65)%, and (73.4 +/- 0.67)%, respectively. Hoechst 33 258 staining found that more nuclear pyknosis and aggregation occurred in the lactacystin injury group, but less occurred in EGCG pretreated groups. FCM indicated that the apoptosis ratio was reduced by EGCG pretreatment. It was 3.0%, 60.4%, 59.8%, 57.5%, and 38.6%, respectively in the normal control group, the lactacystin injury group, and EGCG pretreated groups (at the final concentration of 5, 10, and 50 micromol/L, respectively).
CONCLUSIONEGCG could attenuate lactacystin induced PC 12 cell injury.
Acetylcysteine ; adverse effects ; analogs & derivatives ; Animals ; Apoptosis ; drug effects ; Catechin ; analogs & derivatives ; pharmacology ; Flow Cytometry ; PC12 Cells ; Rats
9.Protective effect of epigallocatechin gallate against sperm abnormality in mice.
Liu-Cai SUI ; Yi-Feng GE ; Juan-Juan XU ; Rong-Hua WU ; Hai-Yan FU ; Bing YAO
National Journal of Andrology 2014;20(12):1068-1072
OBJECTIVETo investigate the protective effect of epigallocatechin gallate (EGCG) on mouse sperm in vivo.
METHODSA total of 64 six-week-old male Kuming mice were randomly divided into eight groups of equal number to be treated with normal saline (negative control), Cyclophosphamide (CP) at 30 mg/kg (positive control), and CP followed by EGCG (experimental) at 20, 40, and 80 mg/kg, respectively, given every other day for 10 days. At 4 and 5 weeks after treatment, the bilateral testes of the mice were harvested for examination of sperm abnormality.
RESULTSEGCG did not increase the rate of CP-induced sperm abnormality in the mice, but reduced it instead with the prolonged time of treatment.
CONCLUSIONEGCG protects mouse sperm in vivo.
Animals ; Catechin ; analogs & derivatives ; pharmacology ; Cyclophosphamide ; toxicity ; Male ; Mice ; Mutagens ; toxicity ; Random Allocation ; Spermatozoa ; drug effects ; Time Factors
10.Advance in anticancer studies on catechins and their derivatives.
Jie ZHOU ; Zheng-li GONG ; Kai ZHANG ; Yang-ping DING
China Journal of Chinese Materia Medica 2012;37(17):2510-2518
Experimental studies on animals discovered that catechins have a notable inhibitory effect on the formation and development of tumors in different organs. Studies on their mechanism showed that catechins could inhibit cancer cell invasion and proliferation, angiogenesis and metastasis and induce cancer cell apoptosis through anti-oxidation and by inhibiting related enzyme activities and cancer cell signal transmission. This essay provides an overview of correlation between tea drinking and cancer prevention, various mechanisms of catechins and their derivatives such as biochemical cancer prevention and anticancer and looks into the future of catechins.
Animals
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Antineoplastic Agents, Phytogenic
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chemistry
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pharmacology
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Catechin
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chemistry
;
pharmacology
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Humans
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Neoplasms
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drug therapy
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metabolism
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prevention & control
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Plant Extracts
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chemistry
;
pharmacology
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Signal Transduction
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drug effects