1.Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2.
Atcharaphan WANLOP ; Chalobol WONGSAWAD ; Pongphol PRATTAPONG ; Pheravut WONGSAWAD ; Thapana CHONTANANARTH ; Jong Yil CHAI
The Korean Journal of Parasitology 2017;55(4):445-449
The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32–34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.
Carps
;
Databases, Nucleic Acid
;
Goldfish
;
Metacercariae*
;
Parasites
;
Poecilia
;
Prevalence*
;
Spine
;
Thailand*
;
Zebrafish
2.Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea
Woon Mok SOHN ; Byoung Kuk NA ; Shin Hyeong CHO ; Soon Won LEE
The Korean Journal of Parasitology 2019;57(6):681-690
The infection status of digenetic trematode metacercariae (DTM) was investigated in fishes from coastal lakes in Gangwon-do, the Republic of Korea (Korea). All fishes collected in 5 lakes were examined with the artificial digestion method. More than 10 species, i.e., Metagonimus spp., Pygidiopsis summa, Centrocestus armatus, Metorchis orientalis, M. taiwanensis, Clinostomum complanatum, Echinostoma spp., Stictodora spp., Diplostomum sp. and Diplostomid No. 1. by Morita (1960), of DTM were detected in fishes from 5 coastal lakes in Gangwon-do. Metagonimus spp. metacercariae were found in 52 (41.3%) out of 126 sea rundace, Tribolodon hakonensis, from 5 lakes, and their density was 14.6 per fish infected. P. summa metacercariae were detected in 48 (84.2%) out of 57 mullets from 5 lakes, and their density was 316 per fish infected. C. armatus metacercariae were detected in 7 (14.6%) T. hakonensis and 3 (15.0%) Tridentiger brevispinis from Hyang-ho, and 5 (19.2%) Acanthogobius flavimanus from Gyeongpo-ho. Stictodora spp. metacercariae were found in 4 fish species, i.e., Tridentiger obscurus, Tridentiger trigonocephalus, Chelon haematocheilus, and Acanthogobius lactipes, from Gyeongpo-ho. Total 15 C. complanatum metacercariae were detected in 2 (9.1%) crucian carp, Carassius auratus, from Songji-ho. M. taiwanensis metacercariae were found in T. hakonensis from Hyang-ho and Gyeongpo-ho and in Pseudorasbora parva from Gyeongpo-ho. Total 11 M. orientalis metacercariae were detected in 3 (6.3%) T. hakonensis from Hyang-ho. From the above results, it was confirmed that various species of DTM are infected in fishes from coastal lakes in Gangwon-do, Korea.
Carps
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Digestion
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Echinostoma
;
Fishes
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Gangwon-do
;
Goldfish
;
Heterophyidae
;
Korea
;
Lakes
;
Metacercariae
;
Methods
;
Republic of Korea
;
Smegmamorpha
4.Properties of Israeli carp ( Cyprinus carpio L . ) sIgM + and sIgM - Cells in a Cell - mediated Immunity.
Min Soon CHOI ; Sang Hoon CHOI ; Jong Kyu WOO ; Eun Joo CHUNG ; Min Jung LEE ; Kwan Ha PARK ; Sung Woo PARK ; Young Gill KIM ; Jong Bae KIM ; Seung Bae LEE
Korean Journal of Immunology 1997;19(3):319-326
No abstract available.
Carps*
;
Killer Cells, Natural
6.Research on the influence of light with different wavelength on the motion behavior of carp robots.
Yong PENG ; Yang ZHAO ; Qian ZHANG ; Zhiyong BAO ; Xiaoyue LIU ; Hui ZHANG ; Jianing LIU ; Yudong WEN
Journal of Biomedical Engineering 2021;38(4):647-654
In order to study the effect of light with different wavelengths on the motion behavior of carp robots, phototaxis experiment, anatomical experiment, light control experiment and speed measurement experiment were carried out in this study. Blue, green, yellow and red light with different wavelength were used to conduct phototaxis experiments on carp to observe their movement behavior. By dissecting the skull bones of the carp to determine the appropriate location to carry the light control device, we independently developed a light control carrying device which was suitable for any illumination intensity environment. The experiment of the light-controlled carp robots was carried out. The motion behavior of the carp robot was checked by using computer binocular stereo vision technology. The motion trajectory of the carp robot was tracked and obtained by applying kernel correlation filter (KCF) algorithm. The motion velocity of the carp robot at different wavelengths was calculated according to their motion trajectory. The results showed that carps' sensitivity to different light changed from strong to weak in the order of blue, red, yellow and green, so that using light with different wavelengths to control the speed of the carp robot has certain laws to follow. A new method to avoid brain damage in carp robots control can be provided in this study.
Algorithms
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Animals
;
Carps
;
Motion
;
Phototaxis
;
Robotics
7.Toxic Hepatitis Associated with Carp Juice Ingestion.
Hye Suk SON ; Guil Sun KIM ; Seung Woo LEE ; Sang Bum KANG ; Jong Tae BACK ; Soon Woo NAM ; Dong Soo LEE ; Byung Min AHN
The Korean Journal of Hepatology 2006;12(1):103-106
The potential hepatotoxicity of herbal remedies and/or health foods is usually ignored in daily life. There have been cases showing the toxic hepatitis and renal failure associated with the ingestion of raw carp bile. We experienced a case of toxic hepatitis without any evidence of renal failure that was associated with carp juice ingestion. The clinical manifestations were characterized by nausea and vomiting after the ingestion of carp juice for 3 months. The diagnosis of toxic hepatitis was made on the basis of the patient's history, laboratory data, RUCAM (Russel Uclaf Causality Assessment) and the results of ultrasonography guided liver biopsy. The patient showed rapid improvement after instituting supportive therapy.
Male
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Korea
;
Humans
;
Hepatitis, Toxic/*etiology
;
*Carps
;
*Bile
;
Animals
;
Adult
8.Contents of Lead, Cadmium and Zinc in Bone, Muscle and Fin of Carassius carassius from Middle Stream of Nakdong River, Korea.
Doohie KIM ; Yong Gu KIM ; Bong Ki JANG
Korean Journal of Preventive Medicine 1988;21(2):307-319
In order to determine the contents of lead, cadmium and zinc, the tissues of the crussian carp, Carassius carassius, collected from the middle streams of the Nakdong river were examined. During the dry season from 8 to 15, March, 1987, six loci were selected to sample the fish and river water; five of them were the midstreams of the Nakdong river i.e., the vicinities of the Andong dam, the Nakdong bridge, the Waegwan bridge, Gangjung and Gaepori, the other one was the Kumho river around the Paldal bridge(see Fig.1). The microanalyses of lead and cadmium contents were undertaken by the atomic absorption spectrophotometer(Model IL-551) connected with CTF-IL 655, while that of zinc by the flame method with IL-551 only. The contents of lead and cadmium in water sampled from Gaepori distant from about 30km downward from the junction with the Kumho river were about 1.5 times higher than those from Gangjung distant about 0.5km upward from the junction, and the content of zinc from Gaepori was slightly higher than that from Gangjung. However, the contents of lead and cadmium in water sampled from Gaepori were three fifth of those from the vicinity of Paldal bridge far about 2km upperward from the junction. In the other words, the contents of lead and cadmium in the samples from the vicinity of the Paldal bridge were about 2.5 to 3 times higher than those from the Gangjung and 5 times higher than those from the Andong dam. The contents of the heavy metals in the tissues of Carassius carassius were relatively consistent to those of the aquatic environments were the fish were collected, with higher contents in the tissues of the crussian carp collected from the vicinity of Gaepori and the Paldal bridge which were so much polluted than in those from other loci in upper stream from the junction. And the contents of lead and cadmium in tissues were positively correlated with the age of the fish, however, that zinc was not. The contents of lead in bone of crussian carp from the vicinity of the Paldal bridge in the group of 0-1 year old fish were similar to those of 4-5 years old fish from Gaepori, and higher than those of 3-4 years old fish collected from the upper stream of the junction. It is likely that fishing from Gaepori and the paldal bridge is not recommended, and all the industrial producers have to pay intensive attention to the water pollution due to the sewage disposal.
Absorption
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Cadmium*
;
Carps*
;
Gyeongsangbuk-do
;
Korea*
;
Metals, Heavy
;
Rivers*
;
Seasons
;
Sewage
;
Water
;
Water Pollution
;
Zinc*
9.The Carpometacarpal Boss
The Journal of the Korean Orthopaedic Association 1976;11(3):455-457
The carpometacarpal boss is a bone prominence involving the carpometacarpal joints of the index and long fingers. In 1931 Fiolle described a bone lesion located the dorsum of his right wrist. He initially believed this to be primarily a carpal lesion and then called it a carpe bossu (carpal boss). The cause of the lesion has been thought to be the result either of a childhood fracture, or of an occupational strain or of periostitis secondary to the pull of the extensor carpi radialis brevis tendon insertion. A congenital predisposition, os styloideum, has also been suggested. We experienced three patients possessing a total of five lesions which showed that repeated mechanical stress in four and os styloideum in one were implicated in the etiology of this deformity.
Carpometacarpal Joints
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Carps
;
Congenital Abnormalities
;
Fingers
;
Humans
;
Periostitis
;
Stress, Mechanical
;
Tendons
;
Wrist
10.Three-dimensional reconstruction of carp brain tissue and brain electrodes for biological control.
Yong PENG ; Aidi WANG ; Tingting WANG ; Jinglong LI ; Zhanqiu WANG ; Yang ZHAO ; Zilin WANG ; Zheng ZHAO
Journal of Biomedical Engineering 2020;37(5):885-891
In order to accurately implant the brain electrodes of carp robot for positioning and navigation, the three-dimensional model of brain structure and brain electrodes is to be proposed in the study. In this study, the tungsten electrodes were implanted into the cerebellum of a carp with the aid of brain stereotaxic instrument. The brain motor areas were found and their three-dimensional coordinate values were obtained by the aquatic electricity stimulation experiments and the underwater control experiments. The carp brain and the brain electrodes were imaged by 3.0 T magnetic resonance imaging instrument, and the three-dimensional reconstruction of carp brain and brain electrodes was carried out by the 3D-DOCTOR software and the Mimics software. The results showed that the brain motor areas and their coordinate values were accurate. The relative spatial position relationships between brain electrodes and brain tissue, brain tissue and skull surface could be observed by the three-dimensional reconstruction map of brain tissue and brain electrodes which reconstructed the three-dimensional structure of brain. The anatomical position of the three-dimensional reconstructed brain tissue in magnetic resonance image and the relationship between brain tissue and skull surface could be observed through the three-dimensional reconstruction comprehensive display map of brain tissue. The three-dimensional reconstruction model in this study can provide a navigation tool for brain electrodes implantation.
Animals
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Brain/diagnostic imaging*
;
Carps
;
Electrodes
;
Electrodes, Implanted
;
Imaging, Three-Dimensional
;
Magnetic Resonance Imaging