1.E-cadherin and Cytokeratin Subtype Profiling in Effusion Cytology.
Joungho HAN ; Mi Kyung KIM ; Seok Jin NAM ; Jung Hyun YANG
Journal of Korean Medical Science 2004;19(6):826-833
Diagnostic utility of E-cadherin (E-CD) and cytokeratin (CK) subtype profiling in effusion cytology was investigated, employing immunocytochemistry on cellblock sections available from 211 metastatic carcinomas (MC), 6 mesotheliomas and 73 reactive mesothelial hyperplasias (MH). E-CD and monoclonal carcinoembryonic anti-gen (mCEA) stained 85% (120/141) and 65% (138/211) of MC, respectively. E-CD staining of MC was frequently heterogeneous (76/120) and absent in all anaplastic carcinomas (0/2). E-CD stained none (0/57) of MH while mCEA and epithelial membrane antigen (EMA) stained 12% (9/73) and 32% (16/32) of MH, respectively. Of 6 mesotheliomas, E-CD focally stained in 2 while mCEA stained none and EMA stained all. CK20 and CK17 stained none of MH or mesotheliomas. CK20 stained 15% of MC and CK 17 stained 22% of MC. CK5/6 and high molecular weight CK stained all mesotheliomas, 56% and 88% of MH, 26% and 39% of MC, respectively. MC showed predominant CK7+/20-expression, with the exceptions of MC from mucinous type of colon/rectum and ovary showing predominant CK20 positive. E-CD may be a useful positive marker for MC in effusion cytology, although it may focally stain in some mesotheliomas. Any positive staining for CK20 of MC suggests MC from the gastrointestinal tract or ovary among others.
Cadherins/*metabolism
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Carcinoma/diagnosis/*metabolism/*secondary
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Comparative Study
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Diagnosis, Differential
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Epithelium/*metabolism/*pathology
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Humans
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Hyperplasia/metabolism
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Immunohistochemistry/methods
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Keratin/*metabolism
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Mesothelioma/diagnosis/*metabolism
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Tumor Markers, Biological/*metabolism
2.Colon hepatoid adenocarcinoma with live metastasis.
Jie ZHANG ; Xiao-jing LI ; Hao-hua TENG
Chinese Journal of Pathology 2005;34(4):249-250
Adenocarcinoma
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metabolism
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secondary
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surgery
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Carcinoembryonic Antigen
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metabolism
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Carcinoma, Hepatocellular
;
metabolism
;
secondary
;
surgery
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Colectomy
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Colonic Neoplasms
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metabolism
;
pathology
;
surgery
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Diagnosis, Differential
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Humans
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Keratin-18
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metabolism
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Liver Neoplasms
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metabolism
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secondary
;
surgery
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Male
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Middle Aged
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alpha-Fetoproteins
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metabolism
3.Metastases of breast cancer to female genital tract: report of 2 cases.
Ding-bao CHEN ; Li-hua QIAN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(3):190-191
Breast Neoplasms
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metabolism
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pathology
;
surgery
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Carcinoma, Ductal, Breast
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metabolism
;
secondary
;
surgery
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Carrier Proteins
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metabolism
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Diagnosis, Differential
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Endometrial Neoplasms
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metabolism
;
secondary
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Female
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Glycoproteins
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metabolism
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Humans
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Immunohistochemistry
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Keratin-7
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metabolism
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Mastectomy, Modified Radical
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Middle Aged
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Ovarian Neoplasms
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metabolism
;
secondary
4.Sigmoid colon metastasis from hepatocellular carcinoma.
Dong Jun YOO ; Young Hwa CHUNG ; Yoon Seon LEE ; Sung Eun KIM ; Young Joo JIN ; Yu Mi LEE ; Mi Jung KIM
The Korean Journal of Hepatology 2010;16(4):397-400
Hepatocellular carcinoma (HCC) is a major health problem worldwide, and it has a poor prognosis. Extrahepatic metastasis from HCC is not unusual, with direct invasion representing the main spreading mode. Sites that are frequently involved are the lung, bone, and lymph nodes. There are few reports of HCC invading the distant gastrointestinal tract, especially hematogenously. Herein we report a case of sigmoid colon metastasis from HCC. The patient was diagnosed with HCC and treated with transcatheter arterial chemoembolization (TACE). Eighteen months after TACE the patient presented with abdominal pain on the left lower quadrant, and a CT scan showed an enhanced mass on the sigmoid colon. Immunohistochemical staining revealed that a tumor cell was positive for polyclonal carcinoembryonic antigen and weakly positive for hepatocyte antigen, supporting the diagnosis of HCC metastasis. The patient underwent anterior resection for the metastatic HCC.
Carcinoembryonic Antigen/metabolism
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Carcinoma, Hepatocellular/*diagnosis/pathology/*secondary
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Chemoembolization, Therapeutic
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Humans
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Liver Neoplasms/*pathology/therapy
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Male
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Middle Aged
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Sigmoid Neoplasms/*diagnosis/*secondary/ultrasonography
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Tomography, X-Ray Computed
5.Primary clear cell carcinoma of nasal cavity: report of a case.
Peng LI ; Wei-hua YIN ; Xiu-juan YAO ; Li WAN ; Guo-rong CHEN
Chinese Journal of Pathology 2011;40(1):52-53
Adenocarcinoma, Clear Cell
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metabolism
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pathology
;
surgery
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Adult
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Carcinoma
;
metabolism
;
pathology
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Carcinoma, Mucoepidermoid
;
metabolism
;
pathology
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Carcinoma, Renal Cell
;
metabolism
;
pathology
;
secondary
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Diagnosis, Differential
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Humans
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Keratins
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metabolism
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Male
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Nasal Cavity
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Nose Neoplasms
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metabolism
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pathology
;
surgery
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S100 Proteins
;
metabolism
6.Expression pattern of E-cadherin and p120-catenin in infiltrating lobular carcinoma and ductal carcinoma of the breast and its significance.
Lan LI ; Xiao-feng BI ; Xin XU ; Xiu-yun LIU ; Gui-hua SHEN ; Lei GUO ; Yan-ling YUAN ; Fang WANG ; Ming-rong WANG ; Hong-ying YANG
Chinese Journal of Oncology 2010;32(4):273-277
OBJECTIVETo determine how patients with infiltrating lobular carcinoma (ILC) differ from patients with the more common infiltrating ductal carcinoma (IDC), and observe the different expression patterns of E-cadherin and p120-catenin proteins in both ILCs and IDCs.
METHODSThe patients with ILC admitted to our hospital from Jan 1999 to Dec 2006 and patients with IDC from Jan 2000 to Dec 2000 were included in this study. All their pathological slides were reviewed, and their clinical data and treatment variables were analyzed retrospectively. Then the expression patterns of E-cadherin and p120-catenin proteins in both ILCs and IDCs were detected by immunohistochemistry on tissue microarray.
RESULTSThe 5-year overall survival was 81.7% for ILCs and 79.1% for IDCs (P = 0.055). The 5-year disease-free survival was 61.8% for ILCs and 83.7% for IDCs (P < 0.001). Cytoplasmic localization of p120-catenin and loss of E-cadherin expression were more common in ILCs than in IDCs. The complete losses of E-cadherin in ILCs and IDCs were 55.6% (20/36) and 20.4% (45/221, P < 0.001), respectively. The p120-catenin showed a diffuse cytoplasmic localization in 66.7% (24/36) of ILCs and 16.3% (36/221) of IDCs (P < 0.001). Interestingly, the cytoplasmic localization of p120-catenin was clearly associated with the absence of E-cadherin expression in ILCs (P = 0.002), cytoplasmic localization of p120-catenin and absence of E-cadherin expression were observed 55.6% (20/36) in ILCs compared with 4.1% (9/221) in IDCs (P < 0.001).
CONCLUSIONILC has several specific biological and prognostic characteristics which are different in IDC. Different expression patterns of E-cadherin and p120-catenin proteins can be helpful to recognize ILC from IDC.
Bone Neoplasms ; secondary ; Breast Neoplasms ; metabolism ; pathology ; Cadherins ; metabolism ; Carcinoma, Ductal, Breast ; metabolism ; pathology ; secondary ; Carcinoma, Lobular ; metabolism ; pathology ; secondary ; Catenins ; metabolism ; Cytoplasm ; metabolism ; Diagnosis, Differential ; Disease-Free Survival ; Female ; Follow-Up Studies ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms ; secondary ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Retrospective Studies ; Survival Rate
7.Malignant melanoma of the back metastatic to thyroid gland: report of a case.
Cheng-lin FU ; Xian-tu ZHANG ; Jin-na ZHANG
Chinese Journal of Pathology 2011;40(2):121-122
Aged
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Back
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Carcinoma, Medullary
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metabolism
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pathology
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Diagnosis, Differential
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Female
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Humans
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Melanoma
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metabolism
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pathology
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secondary
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surgery
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Melanoma-Specific Antigens
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metabolism
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S100 Proteins
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metabolism
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Skin Neoplasms
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metabolism
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pathology
;
surgery
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Thyroid Neoplasms
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metabolism
;
pathology
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secondary
;
surgery
8.Impact of micrometastasis in pathologically negative lymph node on staging and prognosis of non-small cell lung cancers.
Ruheng ZHENG ; Di GE ; Yulei QIAO ; Meixin SHI
Chinese Journal of Oncology 2002;24(1):41-43
OBJECTIVETo study the influence of micrometastasis in lymph node on staging and prognosis of non-small-cell lung cancer (NSCLC).
METHODSIn 39 NSCLC patients, micrometastasis in pathologically negative lymph nodes were tested through immunohistochemical cytokeratin (CK) analysis and the relationship between CK(+) and staging, survival were analyzed.
RESULTSIn these 39 patients, the survival of CK(+) and CK(-) patients were 32 months and 48 months respectively (P = 0.0178). Multivariate analysis of Cox regression model showed: clinical stage (P = 0.0288) and relapse or metastasis (P = 0.0053) affected the prognosis while micrometastasis in lymphnodes (P = 0.7740) did not.
CONCLUSIONThe detection of micrometastasis in the lymphnodes may serve as a supplement to the present staging system for lung cancer. Even though the prognosis of patients with micrometastasis being poorer than those without, micrometastasis in the lymph nodes should not be regarded as an independent prognostic factor.
Adult ; Aged ; Carcinoma, Non-Small-Cell Lung ; diagnosis ; metabolism ; secondary ; Female ; Humans ; Keratins ; metabolism ; Lung Neoplasms ; diagnosis ; metabolism ; pathology ; Lymph Nodes ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis
9.Pathologic diagnosis and differential diagnosis of small cell neuroendocrine carcinoma of kidney.
Ai-tao GUO ; Heng HUANG ; Li-xin WEI
Chinese Journal of Pathology 2012;41(8):538-542
OBJECTIVETo study the clinicopathologic features and histologic differential diagnosis of small cell neuroendocrine carcinoma (SmCC) of kidney.
METHODSThe clinicopathologic features of 12 cases of SmCC of kidney encountered during the period from 1999 to 2010 were retrospectively reviewed.
RESULTSSix cases of primary and 6 cases of metastatic SmCC involving kidney were identified. Amongst the primary renal SmCC, 2 were located in renal parenchyma and 4 in renal pelvis. Chest X-ray showed negative findings. Five of them underwent radical nephrectomy. On gross examination, the tumor was located centrally around the renal pelvis in 4 cases and peripherally in renal parenchyma in 1 case. On the other hand, 4 of the 6 cases of metastatic SmCC were discovered during therapy for pulmonary SmCC. Two of these patients presented with abdominal pain and gross hematuria, with lung and renal tumor masses identified simultaneously. The diagnosis of all the 6 cases of metastatic SmCC was confirmed by fine needle aspiration biopsy. Microscopically, pure SmCC was demonstrated in the 2 cases of primary renal parenchymal SmCC and 6 cases of metastatic SmCC. The 4 primary renal pelvic SmCC coexisted with urothelial carcinoma component. On immunohistochemical study, all cases were positive for cytokeratin, synaptophysin and CD56. All metastatic cases and 4 primary cases were also positive for TTF-1. Of six patients with primary SmCC two died 4 and 9 months after operation, and two were alive with a follow-up of 25 and 138 months, respectively. Five of six cases with metastatic SmCC died 3 - 8 months after diagnosis. The other 3 cases were failed to follow-up.
CONCLUSIONSBoth primary and metastatic SmCC can be found in the kidney. Although rare, primary SmCC is located either in renal parenchyma or in pelvis. The diagnosis of SmCC relies on morphologic examination and immunohistochemical study. TTF-1 immunostaining cannot reliably distinguish primary from metastatic SmCC in kidney. Correlation with clinicoradiologic findings and demonstration of coexisting urothelial carcinoma component (if any) is helpful in delineation of the tumor origin.
Adult ; Aged ; CD56 Antigen ; metabolism ; Carcinoma, Neuroendocrine ; metabolism ; pathology ; secondary ; surgery ; Carcinoma, Renal Cell ; metabolism ; pathology ; Carcinoma, Small Cell ; metabolism ; pathology ; secondary ; surgery ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Humans ; Keratins ; metabolism ; Kidney Neoplasms ; metabolism ; pathology ; secondary ; surgery ; Lung Neoplasms ; pathology ; secondary ; Lymphoma ; metabolism ; pathology ; Male ; Middle Aged ; Nephrectomy ; Nuclear Proteins ; metabolism ; Retrospective Studies ; Sarcoma, Ewing ; metabolism ; pathology ; Synaptophysin ; metabolism ; Thyroid Nuclear Factor 1 ; Transcription Factors ; metabolism ; Treatment Outcome ; Wilms Tumor ; metabolism ; pathology
10.Cervical carcinoid with high-grade intraepithelial neoplasia: report of a case.
Hai LI ; Fang BAO ; Yu-fei LI ; Yi-long DAI ; Ying XIANG ; Zhi-hong ZHANG
Chinese Journal of Pathology 2013;42(5):347-348
Adult
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Breast Neoplasms
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metabolism
;
pathology
;
secondary
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Carcinoid Tumor
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metabolism
;
pathology
;
surgery
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Carcinoma, Adenoid Cystic
;
pathology
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Carcinoma, Lobular
;
metabolism
;
pathology
;
secondary
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Cervical Intraepithelial Neoplasia
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metabolism
;
pathology
;
surgery
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Chromogranin A
;
metabolism
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Diagnosis, Differential
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Female
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Humans
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Hysterectomy
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Keratins
;
metabolism
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Neoplasms, Multiple Primary
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metabolism
;
pathology
;
surgery
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Ovarian Neoplasms
;
metabolism
;
pathology
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Sex Cord-Gonadal Stromal Tumors
;
metabolism
;
pathology
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Synaptophysin
;
metabolism
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Uterine Cervical Neoplasms
;
metabolism
;
pathology
;
surgery