1.Distribution of hepatitis B virus genotypes and subgenotypes among chronically infected patients in Xinjiang Uighur..
Xiao-Feng SUN ; Shu-Juan WEN ; Hui LI ; Jin-Lin HOU ; Yue-Xin ZHANG ; Zhan-Hui WANG ; Yu-Jian ZHENG
Chinese Journal of Hepatology 2009;17(2):88-90
OBJECTIVETo investigate the distribution of Hepatitis B virus genotypes and subgenotypes among patients with chronic hepatitis B in Xinjiang Uighur.
METHODSThe HBV genotypes and subgenotypes were analyzed by PCR-restriction fragment length polymorphism in 109 patients with chronic hepatitis B.
RESULTSTwo HBV genotypes, genotype C (45.9%) and genotype C/D (29.4%) were prevalent, genotype B (8.3%) and genotype D (16.5%) were also found in Xinjiang Uighur. Genotype C had two subgenotypes, C1 (54%) and C2 (46%). Genotype B had only one subgenotype, i.e. Ba. The subgenotype C2 was associated with cirrhosis and hepatocellular carcinoma.
CONCLUSIONIn Uygurs, the most common HBV genotypes were C and C/D, and the subgenotype C2 was associated with cirrhosis and hepatocellular carcinoma.
Carcinoma, Hepatocellular ; virology ; Genotype ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; virology ; Humans ; Liver Neoplasms ; virology
6.Distribution of Hepatitis B Virus Genotypes according to the Clinical Outcomes in Patients with Chronic Hepatitis B Virus Infection in Jeju Island.
Bum Joon KIM ; Byung Cheol SONG
The Korean Journal of Gastroenterology 2003;42(6):496-501
BACKGROUND/AIMS: The genotype of hepatitis B virus (HBV) has been suggested to correlate with the clinical outcome of HBV infection. We analyzed the distribution of HBV genotypes according to the clinical outcomes of HBV infection in Jeju island. METHODS: A total of 145 HBsAg-positive samples were enrolled. To identify specific patterns of HBV genotypes, we performed restriction fragment length polymorphism (RFLP). In the case that typical restriction pattern of RFLP was not determined, phylogenetic analysis was performed. RESULTS: RFLP analysis was possible in 65 patients. Clinical diagnoses of the 65 patients with chronic liver disease were as follows: HBeAg-positive healthy carrier (HBeAg+, anti-HBe, HBV DNA+, transaminase normal; n=20); Inactive HBsAg carrier (n=12); chronic hepatitis B (n=14); liver cirrhosis (n=9); hepatocellular carcinoma (n=10). Sixty-two patients showed a typical restriction pattern by HinfI. However, 3 patients showed a unique restriction pattern by HinfI, which were not reported in the literature. When phylogenetic analysis was performed to classify the genotype of these 3 patients, they were also genotype C. However, all 65 patients showed typical restriction patterns by Tsp509I, which were reported in genotype C. CONCLUSIONS: All chronic HBV infections are genotype C in Jeju island regardless of clinical outcomes.
Carcinoma, Hepatocellular/virology
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Carrier State/virology
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Genotype
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Hepatitis B virus/*genetics
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Hepatitis B, Chronic/complications/*virology
;
Humans
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Liver Cirrhosis/virology
;
Liver Neoplasms/virology
;
Polymorphism, Restriction Fragment Length
Result Analysis
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