BACKGROUND: There has been no general agreement on classifying basal cell carcinoma (BCC), and little is known about the squamous cell differentiations of each subtype of BCC. BCC is a locally-invasive tumor, but its aggressive forms tend to recur and metastasize. OBJECTIVE: We have compared the histolopathological subtypes of BCC by squamous differentiation, with special emphasis on basosquamous carcinoma and the difference between the non- aggressive and aggressive forms of BCC. MATERIALS AND METHOD: A total of 87 BCC specimens were collected from the 7 branch hospitals of The Catholic University of Korea from July 1997 to June 2003. RESULTS: 1. The 87 samples were divided into 6 subtypes: 18 nodular, 19 nodular infiltrative, 12 micronodular, 14 infiltrative, 11 morpheaform, 13 basosquamous. Overall, 18 samples were classified as non- aggressive and the remaining 69 as aggressive. 2.In nodular-infiltrative BCC and basosquamous carcinoma, squamous cell differentiation was significantly more prominent than in the nodular BCC. CONCLUSION: Squamous cell differentiation should be considered as one of the aggressive markers, especially in nodule-forming BCC.
Carcinoma, Basal Cell* ; Carcinoma, Basosquamous ; Cell Differentiation* ; Hospitals, Satellite ; Korea

An 89-Year-old female patient had had a pea-sized encrusted ulcer on the vermillion border of her right upper lip for 4 years. Histopathological features of the biopsy specimen consisted of tumor islands with peripheral, palisading basaloid cells, some of which had become transformed into atypical, dyskeratotic squamous cells with ample eosinophilic cytoplasm in their centers. Total excision was advised.
Aged, 80 and over ; Biopsy ; Carcinoma, Basal Cell ; Carcinoma, Basosquamous* ; Cytoplasm ; Eosinophils ; Female ; Humans ; Islands ; Lip ; Ulcer

Basosquamous carcinoma is a rare epithelial neoplasm, mostly occurring on the head and neck area. There are few reports of basosquamous carcinoma on the finger. Here, the authors experienced treatment of basosquamous carcinoma on the finger in a radiologist. Treatment was successful by the wide excision and the cross-finger flap operation with a split-thickness skin graft and K-wire fixation. The rare finger basosquamous carcinoma case in our study is likely to be linked with radiation. Considering of the high reliance of C-arm during hand surgeries, we think that the hand of the surgeons should be more strictly protected.
Carcinoma, Basosquamous* ; Fingers ; Hand* ; Head ; Neck ; Neoplasms, Glandular and Epithelial ; Radiation Exposure* ; Skin ; Surgeons ; Transplants

PURPOSE: To report a rare case of basosquamous carcinoma of the eyelid, an aggressive tumor with a higher tendency for recurrence and metastasis. CASE SUMMARY: An 87-year-old woman presented with a painful mass and bloody exudates at the left lateral lower eyelid. Four years previous, the patient was diagnosed with basosquamous carcinoma of the left lower eyelid after biopsy at another hospital. At that time, she was unable to receive operation because she had suffered from a serious heart condition. With time, the left lower eyelid mass continued to grow and symptoms and signs of pain and bloody exudates appeared. The patient underwent surgery for complete tumor resection and repair and the biopsy of a specimen showed tumor-free margins and no metastasis to distal sites. CONCLUSIONS: Basosquamous carcinoma is a subtype of basal cell carcinoma with aggressive behavior and a higher tendency for recurrence and metastasis. However, our case showed no recurrence with no metastasis to the nearby lymph nodes, vessels, or nerves. We report a case of basosquamous carcinoma of the eyelid at old age that was cured after operative resection.
Aged, 80 and over ; Biopsy ; Carcinoma, Basal Cell ; Carcinoma, Basosquamous ; Exudates and Transudates ; Eyelids ; Female ; Heart ; Humans ; Lymph Nodes ; Neoplasm Metastasis ; Recurrence

Basosquamous carcinoma of the skin is a relatively rare cutaneous neoplasm that has significant metastatic potential and a metastatic rate greater than that of basal cell and squamous cell carcinoma. We describe the use of lymphatic mapping and sentinel lymph node biopsy in a 63-year-old man after identification of basosquamous carcinoma. Sentinel lymph node biopsy, which is a standard tool to detect regional lymphatic metastasis in cutaneous melanoma, has been rarely employed to detect lymphatic metastasis of basosquamous carcinoma. The approach was successful in detecting a regional lymphatic metastasis of two nodal basins with minor morbidity. Sentinel lymph node biopsy may be useful for certain high-risk lesions of basosquamous carcinoma.
Carcinoma, Basosquamous ; Carcinoma, Squamous Cell ; Humans ; Lymphatic Metastasis ; Melanoma ; Middle Aged ; Neoplasm Metastasis ; Nitriles ; Pyrethrins ; Sentinel Lymph Node Biopsy ; Skin

PURPOSE: Basosquamous carcinoma is a rare malignancy, with features of both basal cell carcinoma and squamous cell carcinoma. It is considered as aggressive tumor with a high risk of recurrence and metastases. Authors report a case of basosquamous cell carcinoma. METHODS: A 72 year-old man, who had an erythematous ulcer on his left auricle, described a slow growing lesion, starting at the posterior surface of the superior helix with a steady increase in size during the past 10 years. At operation, auricular cartilage was grossly invaded by the tumor and was, therefore, amputated with tumor-free margins. RESULTS: Histopathologic examination was revealed a basosquamous cell carcinoma. On positron emission tomography/computed tomography (PET/CT) and neck CT were negative for signs of further nodal involvement or metastases to other organs. At follow-up 6 months later, his wounds were noted to be well healed, with no evidence of local recurrence or identifiable metastases. CONCLUSION: Because basosquamous cell carcinoma has a significant potential to recur and metastasize, surgical excision for this type of carcinoma should be more extensive than that performed for conventional basal cell carcinoma or squamous cell carcinoma. In addition, regional lymph nodes should be monitored and close follow-up should be carried out.
Carcinoma, Basal Cell ; Carcinoma, Basosquamous ; Carcinoma, Squamous Cell ; Ear Cartilage ; Electrons ; Follow-Up Studies ; Lymph Nodes ; Neck ; Neoplasm Metastasis ; Recurrence ; Ulcer

OBJECTIVETo explore the inhibitory effect of matrix metalloproteinase-2 (MMP-2) gene silencing in vitro and in vivo on the invasion and growth of laryngeal cancer cells.

METHODSsiRNA recombinant lentivirus targeting MMP-2 gene was transfected into Hep-2 cells, and MMP-2 protein expression was analyzed consequently by using western-blot. Invasive properties of transfectants were evaluated by Boyden assay. In addition, the lentivirus was intratumorally injected in a model of the grafted nude mouse and the morphological changes of transfectants were examined by transmission electron microscope. Finally, cell proliferation in xenografts was measured by immunolabeling of proliferating cell nuclear antigen (PCNA).

RESULTSOver 90% of target cancer cells were found to be transfected by MMP-2-RNAi-Lentivirus. Western-blot analysis revealed that none of transfectants expressed MMP-2 protein whereas most untreated cancer cells exhibited positive protein expression. Significant differences were found between the treated and untreated groups regarding the number of transfectants penetrating through an artificial basement in a Boyden chamber (12 +/- 4 vs 35 +/- 6, x +/- s, t = 14.492, P < 0.01), and the average value of weight [(1.186 +/- 0.225) g vs [(2.127 +/- 0.344) g] and volume [(0.974 +/- 0.216) cm3 vs (1.618 +/- 0.272) cm3] of the grafted tumors (t was 7.094 and 5.684, P < 0.01). The overall tumor inhibitive rate was about 44.2%. Transmission electron microscope showed an obviously decreased invasive feature of transfectants. Finally, the percentages of transfectants immunolabeled for PCNA were significantly lower in the treated group (49.588 +/- 6.995) than those (71.434 +/- 7. 043) in control one (t = 9. 573, P < 0.01).

CONCLUSIONSThe invasion, growth and proliferation of laryngeal cancer can be inhibited by siRNA mediated MMP-2 gene silencing. These data strongly suggest that MMP-2 gene silencing by siRNA technology could be a promising approach to cancer therapy.

Animals ; Carcinoma, Basosquamous ; genetics ; pathology ; Hep G2 Cells ; Humans ; Laryngeal Neoplasms ; genetics ; pathology ; Lentivirus ; genetics ; Matrix Metalloproteinase 2 ; genetics ; Mice ; Mice, Nude ; RNA Interference ; Transfection

PURPOSE: To discuss the clinical course and diagnosis of corneal dysplasia in a xeroderma pigmentosum patient based on a genetic evaluation. CASE SUMMARY: A 42-year-old female visited our clinic for decreased left visual acuity and corneal opacity. She had undergone several surgeries previously due to the presence of basosquamous carcinoma in the left lower eyelid, neurofibroma, and malignant melanoma of the facial skin. The patient showed repeated corneal surface problems, with a suspicious dendritic lesion; however, antiviral therapy was ineffective, and herpes simplex virus polymerase chain reaction results were negative. Despite regular follow-ups, the patient showed neovascularization around the corneal limbus and an irregular corneal surface. We performed corneal debridement with autologous serum eye drops for treatment. The patient's visual acuity and corneal surface improved after the procedure. The impression cytology result was corneal dysplasia. In whole exome sequencing, two pathogenic variants and one likely pathogenic variant of the POLH gene were detected. CONCLUSIONS: This is the first genetically identified xeroderma pigmentosum case with ophthalmological lesions of the eyelid and cornea in Korea. Debridement of the irregular corneal surface and autologous serum eye drop administration in xeroderma pigmentosum could be helpful for improving visual acuity.
Adult ; Carcinoma, Basosquamous ; Cornea ; Corneal Opacity ; Debridement ; Diagnosis ; Exome ; Eyelids ; Female ; Follow-Up Studies ; Humans ; Ichthyosis ; Korea ; Limbus Corneae ; Melanoma ; Neurofibroma ; Ophthalmic Solutions ; Polymerase Chain Reaction ; Simplexvirus ; Skin ; Visual Acuity ; Xeroderma Pigmentosum

OBJECTIVETo investigate the histopathological features of basaloid squamous cell carcinoma of the esophagus, and to explore the ways of its diagnosis, differential diagnosis and treatment.

METHODSThe clinical data and pathological features of 23 cases of esophageal basaloid squamous cell carcinoma were reviewed and analyzed retrospectively.

RESULTSThe tumors were mainly located at the middle third segment of the esophagus. The 1-,2- and 3-year survival rates were 60.9%, 21.7% and 0, respectively.

CONCLUSIONThe basaloid squamous cell carcinoma of the esophagus is highly malignant with poor prognosis. Radical resection combined with radiotherapy and chemotherapy is required.

Adult ; Aged ; Antineoplastic Agents ; therapeutic use ; Carcinoma, Basosquamous ; diagnosis ; therapy ; Carcinoma, Squamous Cell ; diagnosis ; Cisplatin ; administration & dosage ; Combined Modality Therapy ; Diagnosis, Differential ; Esophageal Neoplasms ; diagnosis ; therapy ; Esophagectomy ; methods ; Esophagus ; pathology ; surgery ; Female ; Fluorouracil ; administration & dosage ; Follow-Up Studies ; Humans ; Liver Neoplasms ; secondary ; Lung Neoplasms ; secondary ; Male ; Middle Aged ; Radiotherapy, High-Energy ; Retrospective Studies ; Survival Rate

Adenocarcinoma ; pathology ; Adenoma ; pathology ; Carcinoma, Adenoid Cystic ; epidemiology ; genetics ; metabolism ; pathology ; Carcinoma, Basosquamous ; pathology ; DNA, Mitochondrial ; genetics ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Incidence ; Proto-Oncogene Proteins c-kit ; metabolism ; Salivary Gland Neoplasms ; epidemiology ; genetics ; metabolism ; pathology ; Salivary Glands, Minor ; pathology ; beta-Defensins ; genetics