Two rat hepatic intoxication models by high doses CCl4 and PAR were used. The experimental results showed that the oral administration of Panax Notoginseng (aqueous extract) with the dose of 5g/kg body weight has significantly reduced the serum concentrations of SGOT and SGPT with 29.1% and 43.1% respectively in comparison with that of CCl4 - treated control . In PAR - intoxicated group, Panax Notoginseng with the dose of 5g/kg body weight resulted in a marked decrease of SGOT and SGPT serum concentration 97.0% and 75.5%, respectively, which were significantly different from that of group without Panax Notoginseng. The hepatoprotective effect of Panax Notoginseng with the dose of 5g/kg and Silymarin with the dose of 25mg/kg on both CCl4 and PAR hepatic-intoxication models is the same. In histopathological examinations, Panax Notoginseng has improved CCl4 and PAR induced hepatic injuries
Panax notoginseng ; Carbon Tetrachloride ; poisoning ; liver ; rats ; Animal Experimentation

Animals ; Breath Tests ; methods ; Carbon Isotopes ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Chemical and Drug Induced Liver Injury ; etiology ; physiopathology ; Liver Function Tests ; methods ; Male ; Phenylalanine ; Rats ; Rats, Sprague-Dawley

Acute Disease ; Adult ; Carbon Tetrachloride Poisoning ; diagnosis ; drug therapy ; Humans ; Male

Adjuvants, Immunologic ; therapeutic use ; Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Cell Wall Skeleton ; Chemical and Drug Induced Liver Injury ; therapy ; Male ; Mice

OBJECTIVETo study the proliferation and apoptosis in carbon tetrachloride induced rat liver fibrosis.

METHODSWistar rats were injected subcutaneously with 40% CCl4-olive oil twice weekly for 12 weeks. Liver tissues were obtained at the end of 4, 8, 12 and 16 weeks for histological examination, hydroxyproline (Hyp) assay and proteomic analysis. After two dimension electrophoresis (2-DE), the silver stained gels were analyzed with PDQUEST 2-DE. More than 30 differentially expressed proteins were identified by MALDI-TOF-MS.

RESULTSThe degree of collagen deposition and hydroxyproline content of the fibrotic livers increased continuously during the 12 weeks of CCl4 administration, peaked at the end of week 12 (P < 0.05) and declined significantly at week 16 (P < 0.05). Significant differences were observed in two parameters at each time point between the control and the model group. Meanwhile, dramatic change of hepatic proteome in the model group rats was also seen. Differentially expressed proteins identified by MALDI-TOF-MS were categorized as proliferation-related proteins/enzymes (proliferating cell nuclear antigen p120, p40 and cyclin F ubiquitin-conjugating enzyme 7 UBC7), and apoptosis-related proteins, mainly caspase-12 which was absent in the control rats.

CONCLUSIONProliferation and apoptosis related proteins are expressed dynamically in different stages of rat liver fibrosis induced by CCl4.

Animals ; Apoptosis ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Caspase 12 ; metabolism ; Cell Proliferation ; Hydroxyproline ; metabolism ; Liver Cirrhosis, Experimental ; chemically induced ; metabolism ; Male ; Proteins ; metabolism ; Proteome ; metabolism ; Rats ; Rats, Wistar

Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Fatty Liver ; chemically induced ; drug therapy ; Glycyrrhizic Acid ; therapeutic use ; Ligands ; Male ; Rats ; Rats, Sprague-Dawley

Animals ; Anthraquinones ; therapeutic use ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Drug Therapy, Combination ; Liver Cirrhosis, Experimental ; chemically induced ; drug therapy ; Male ; Pyrazines ; therapeutic use ; Rats ; Rats, Wistar

This experiment was carried out to make an exaluation on the non-invasive near-infrared detection of early hepatocirrhosis. The model of early hepatocirrhosis was established by injecting carbon tetrachloride in mice. Blood and oxygen content in liver tissue were detected with Runman System. The undulated forms of blood and oxygen variation were extracted and analyzed under the software MATLAB. Non-invasive near-infrared detection was found to react sensitively to the variation of the blood and oxygen content in the early hepatocirrhosis. The rhythm of the undulated forms of blood and oxygen in the early hepatocirrhosis was irregular. The main frequency peak of auto-power spectrum was found to move back to the rang 1.0-1.5 Hz and multi-peak was seen. These show that the non-invasive near-infrared detection is of some value in detecting early hepatocirrhosis.
Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Early Diagnosis ; Liver ; blood supply ; Liver Cirrhosis ; chemically induced ; diagnosis ; Mice ; Oximetry ; methods ; Oxygen ; blood ; Regional Blood Flow ; Spectroscopy, Near-Infrared ; methods

OBJECTIVE: To investigate the effect of prostaglandin E1 (PGE1) on the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) in experimental liver fibrosis rats. METHODS: The liver fibrosis model was established by carbon tetrachloride. Rats were divided into a control group and PGE1-treated group. The pathological changes of the liver tissue from the two groups, the semi-quantitative analysis of hepatitic activity in HE stain sections, the pathological image quantitative analysis of the fibrosis degree, TIMP-1 positive cells, and the content of collagen were synthetically analysed. RESULTS: The mark changes of liver pathology in HE stain sections were that the degree of hepatitic activity in the PGE1-treated group was obviously lower than that in the control group (P < 0.05). The fibrosis degree, TIMP-1 positive cells and the collagenous fibers decreased in the PGE1-treated group (P <0.05). CONCLUSION PGE1 has an anti-hepatofibrosis effect in the experimental rats, the inflammation of liver is light, and the proliferation of collagenous fibers can be restrained, whose mechanism is probably associated with the suppression of TIMP-1 expression caused by PGE1.
Alprostadil ; pharmacology ; Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Female ; Liver Cirrhosis, Experimental ; chemically induced ; metabolism ; Male ; Random Allocation ; Rats ; Rats, Wistar ; Tissue Inhibitor of Metalloproteinase-1 ; biosynthesis ; genetics

OBJECTIVE: To determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats. METHODS: A total of 2*10(7) hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4. The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation. The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation. RESULTS: The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation. The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P<0.05). CONCLUSION Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.
Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Heparin ; therapeutic use ; Hepatocytes ; transplantation ; Liver Failure, Acute ; chemically induced ; therapy ; Male ; Rats ; Rats, Sprague-Dawley ; Stem Cell Transplantation ; methods