Objective To summarize and analyze related examinations of chronic hepatitis B so as to find out the best detection method,which could also improve the diagnostic accuracy rate of chronic hepatitis B.Methods The clinical material of 238 patients with chronic hepatitis B (liver disease group) from March 2012 to November 2013 were studied by using the regression analysis,and meanwhile 238 cases of normal healthy people were selected as control group,the two groups' blood routine,routine biochemical indicators and new biochemical indexes were carefully studied and analyzed,and the positive rates and accuracy of using the biochemical indexes to diagnose chronic hepatitis B were also studied.Results The leukocyte count,neutrophil count and platelet count in liver disease group were respectively (5.10 ± 1.79) ×109/L,0.558 4 ± 0.123 2 and(101.38 ± 79.55) × 109/L,which were all significant lower than those in control group[(6.83 ± 1.45) × 109/L,0.617 8 ± 0.090 1 and (172.29 ± 43.22) × 109/L (P＜ 0.05),and lymph cells were 0.367 7 ± 0.121 0 which were significantly more than that in control group (0.303 9 ± 0.064 5) (P ＜ 0.05).The total bilimbin and alanine aminotransferase in liver disease group were (22.47 ± 7.28)μ mol/Land (97.01 ± 14.18) U/L which were significantly higher than those in control group (15.88 ± 5.19) μ mol/Land (29.87 ± 9.16) U/L (P＜ 0.05),while the albumin,albumin and globubin ratio were significantly lower than that in control group [(38.28 ± 5.77) g/L vs.(45.23 ± 2.34) g/L,1.32 ± 0.21 vs.1.59 ± 0.23] (P ＜0.05) ; adenosine deaminase,5'-nudeotidase,total bile acid,leucine aminopeptidase in liver disease group were significantly higher than that in control group [(24.30 ± 13.12) U/L vs.(9.21 ±2.72) U/L,(12.82 ±12.21) U/L vs.(5.22 ±2.39)U/L,(61.28 ±30.09) mol/L vs.(5.70 ±2.78) mol/L,(64.80 ± 19.21) U/L vs.(41.23 ± 11.32) U/L] (P ＜ 0.05),while transferrin and prealbumin (PA) were significantly lower than that in control group [(17.43 ±8.23) μmol/L vs.(32.44 ±6.90) μmol/L,(123.28 ± 22.31) mg/L vs.(324.20 ± 34.88) mg/L] (P ＜ 0.05); PA and ADA's positive rates were 91.7％and 82.3％ respectively,and the areas under the curve of the two groups were 0.92 and 0.95 respectively.Conclusions It is probably of great help and reference for doctors to accurately assess the liver functions,diagnose and treat chronic hepatitis B patients that using blood routine examinations and the conventional biochemical indexes.