ObjectiveTo explore the clinical effect of edaravone combined with Shuxuetong in treatment of elderly patients with acute cerebral infarction.Methods120 cases of acute cerebral infarction,according to the different treatment methods,were divided into two groups.The conventional treatment was given in two groups,the control group plus Chuanqiong hydrochloride injection,and the observation group adopted edaravone and Shuxuetong.The clinical efficacy,activities of daily living(ADL) and neurological deficit score were compared between two groups.ResultsThere were 25 cases of basic recovery,18 cases of significant progress,progress in 12 cases in the observation group,total effective rate was 91.7％ (55/60),the control group was 75.0％ (45/60),the difference was statistically significant ( x2 =6.86,P ＜ 0.05 ).Observation group had statistically significant difference than the control group between ADL and neurological deficit scores ( all P ＜ 0.05 ).ConclusionOn the basis of conventional therapy,the use of edaravone combined with Shuxuetong for acute ischemic stroke patients could significantly promote the recovery of neurological function and was worthy of promotion.

Objective To investigate the method to prevent the displacement of tissue expander implanted between the panniculus carnosus and deep fasia in rabbits .Method 40 rabbits were divided into two groups randomly: the experimental group and the control group .Through an incision on the back , a pocket was made by dissecting between the rabbit panniculus carnosus and deep fasia .The panniculus carnosus at the pocket boundary were sutured with the deep fascia inside the pocket by interrupted 3-0 sutures.A 50 mL kidney-shaped tissue expander was implanted .The expander was inflated with 10 mL saline after the wound was sutured .7 days after implantation , black tattoos were made on the skin around the expander to mark the site of expander .Two months after implantation , the expanders in the experimental group were inflated with 10 mL saline weekly to a total of 140 mL.7 days after implantation the expanders of control group were inflated with 10 mL saline weekly to a total of 140 mL.The incidence of expander displacement , dehiscence of incision , reversal of injection pot and death were recorded .Results There was no expander displacement in the 19 rabbits of experimental group .All the expanders were located within the tattoo area .There was no expander displacement in 11 rabbits of the control group .4 expanders of the control group moved from the back to belly .The difference between them was statistically significant ( P <0.05 ) .The incidence of dehiscence of incision in the experimental group was not significantly lower than that in the control group ( P >0.05 ) .Meanwhile there was no significant difference in the incidences of reversal of injection pot , infections and deaths ( P >0.05 for all ) .Conclusions Sutures between the panniculus carnosus and the deep fascia at the pocket boundary asisted by delayed inflating can prevent the displacement of tissue expander implanted between the panniculus carnosus and deep fasia in rabbits , thus to obtain effective expansion .

Objective To explore the rat's abdominal pressure decending law by applying quantitative analytical technique.Methods Intra-abdominal hypertension rat model is made by Nitrogen aeroperitonerm.Nitrogen is exhausted the abdomen at fixed quantity and fixed time cell.MP-100A-CE type physiological monitor is used to record the changes of intra-abdominal pressure.The pressure numerical average value at different time are recorded,and the volume of Nitrogen exhausted and the pressure of intra-abdominal scatterplot are drawed.Regression analysis is used to study the functional relation between them.Results The regularity of the intra-abdominal pressure decrease was as follows:In Intra-abdominal Hypertension condition,discharge little Nitrogen could cause intra-abdominal pressure decrease greatly.Then the rate of decay is slowed.Intra-abdominal pressure(y)and Nitrogen in the abdomen(y)present decrement exponential function relation,the formula is:y=36.3190?0.9769x,and the pressure-volume index is-0.01.Conclusion Regularity of intra-abdominal pressure decengding is exponential function between intra-abdominal pressure and Nitrogen volume exhausted from the abdomen.

Sixteen strains of champagne yeasts have deen tested for fermentation characteristies. The results show : C_1, C_3, C, have excellent properties such as high fermentation activity, tiny foam, excellent flocculation and tolerance to alcohol and sulfur dioxide. It is very satisfactory to use them in second fermentation of champagne.

Objective To clone and express the cDNA encoding Schistosoma japonicum tropomyosin. Methods The cDNA was amplified by reverse transcription polymerase chain reaction (RT PCR). The PCR products were ligated with pGEM T vectors and then for transformations. After characterization of white clones by agarose gel electrophoresis, endonucleases digestion and PCR, some recombinant plasmids with inserts were used for sequencing. Then the gene was subcloned into prokaryotic expression vector pQE30 and expression was induced by IPTG. Results The PCR products was 823 bp judged by agarose gel electrophoresis and sequencing. A cDNA encoding S japonicum tropomyosin, except for 14 amino acids at the amino terminus and 2 at the carboxyl terminus, has been constructed and cloned successfully. The colony, designated pGSjcTM12, was sequenced and shown to be 91 1% identical at the nuclei acid level and 98.1% identical in deduced amino acid sequence to that of S mansoni tropomyosin. The gene was subcloned into pQE30 and an expressed protein of about 32 kDa was obtained.Conclusion The cloning and expression of the gene encoding S japonicum tropomyosin had been successfully made.

n 3 fatty acids belong to essential fatty acids. It includes linolenic acid, eicosapentaenoic acid ( EPA ) and docosahexaenoic acid ( DHA ). Many researches proved that n 3 fatty acids can suppress many kinds of tumor cells, and defense the occurrence of tumor. This article reviewed the effects and mechanism of n 3 fatty acids in defension and suppression of tumor.

Tumor metastasis to sentinel lymph nodes represents the first step of tumor dissemination in most human cancers and serves as a major prognostic indicator for disease progression.Recent studies have revealed that tumor lymphangiogenesisis correlated with lymph node metastasis in experimental cancer models and in several types of human Cancers.Metastatic tumor cells may continue to promote lymphatic vessel growth even after their metastasis to sentinel lymph nodes,likely promoting further cancer spread.Vascular endothelial growth factor-C(VEGF-C)and VEGF-D are the first specific lymphangiogenesis factor identified,and a large number of clinical studies have shown a correlation between tumor expression of VEGF-C or VEGF-D and lymph node metastasis.Additional tumor lymphangiogenesis factors have been recently identifled,including VEGF-A.Importantly,blockade of the VEGFR-3 pathway by specific antibodies,by soluble receptor constructs,and by small molecule kinase iubibitors efficiently inhibits experimental tumor lymphangiogenesis and metastasis and might also represent a novel therapeutic avenue for the treatment of human cancers.

The author made a deep analysis to the enrollment situation in the College of Continuing Education, Henan University of TCM, in 2008. The analysis showed that the enrollment source of the undergraduate students is better than the junior college student; Enrollment source of the preponderance professions of TCM such as traditional Chinese medicine, Integrated Chinese and Western medicine, acupuncture and moxibustion and Tuina of Chinese medicine, and science of Chinese material medica is better than non-preponderance profession; The enrollment source of local city is better than other cities. Thus we advocate: paying adequate attention to the enrollment of undergraduate students, increasing the teaching level; setting up preponderance professions of TCM, highlighting characteristics of schooling ; digging enrollment source of local area, and expanding enrollment source from other cities.

Objective To study the effects of High frequency two -way jet ventilation on the function respiratory and circulation of patients with Trachea and Carinal reconstruction.Methods Fifty patients with lung cancer,who were scheduled for elective trachea and carinal reconstruction,were intubated with double lumen tube following anesthesia induction and general anesthesia.High frequency ventilation was used on the healthful main bronchus during carinal reconstruction,a Hunsaker tube was inserted 3cm into the healthful main bronchus.HFTJV was applied with the respiratory rate of 120 /min,the ratio of inspiration and expiration E =1:1 and drive pressure of 0.2 -0.25Mpa.Blood gas analysis was made before the anesthesia,15 min following one -lung ventilation,10,20, 30 min following HFTJV and 15min following one -lung ventilation,respectively and monitor MAP,HR,SpO2 , PetCO2 .Results PaO2 in high frequency ventilation increased significantly compared with preoperative (78.0 ± 10.5)mmHg,was respectively (161.4 ±10.2)mmHg,(156.0 ±15.7)mmHg,(153.0 ±15.1)mmHg (P <0.01);PaO2 in high frequency ventilation 30min was (153.0 ±15.1)mmHg (P <0.05).It was lower than single lung venti-lation (front 165.50 ±11.3mmHg)and higher than 100mmHg equally;PaCO2 in the high frequency ventilation was slightly increased compared with OLV 30min (front),and the difference was not statistically significant (P >0.05). Conclusion HFTJV is safe and reliable for Trachea and carinal reconstruction.

Objective To explore the abnormal apoptosis and lupus nephritis pathology and disease activity relationship.Methods Forty patients with LN renal biopsy tissue, cell apoptosis with TUNEL method and counting cell apoptosis index ( AI) , application of SLE activity score ( SLEDAI) 2000 judged the disease activity, and correlation analysis.Results All patients of renal tissue apoptosis detection were positive, apoptotic cells in the glomerulus and renal tubules were scattered distribution, SLEDAI was posi-tively correlated with the AI ( r =0.38, P =0.029).Conclusions Cell apoptosis and remove obstacles exist in Patients with LN, the more the number of apoptotic cells, the more active SLE disease.