1.Genetic relatedness of Candida albicans bloodstream infection clinical isolates in Malaysia
Chhabra-Singh Saranpal ; Pei Pei Chong ; Kee Peng Ng ; Crystale Siew Ying Lim
Malaysian Journal of Microbiology 2015;11(3):294-299
Aims: The aim of this study was to investigate the genetic relatedness of the most prevalent Candida bloodstream
infection (BSI) species in in a Malaysian population via Randomly Amplified Polymorphic DNA-Polymerase Chain
Reaction (RAPD-PCR) fingerprinting.
Methodology and results: The genomic DNA of 43 Candida BSI blood culture samples obtained from Universiti Malaya
Medical Centre (UMMC) was isolated, after which species identification was carried out using PCR with ITS-1 and ITS-4
pan-fungal primers in conjunction with CHROMagar™ Candida. The predominant Candida species in the BSI samples is
Candida albicans (14 out of 43 isolates). RAPD-PCR on these 14 C. albicans clinical isolates was performed using PST
as the arbitrary primer. Data analysis using MEGA found an overall non-relatedness of these 14 clinical isolates
[average similarity coefficient (SAB) value 0.733±0.172]. Following in-depth analysis, five of the 14 isolates were
observed to be identical (SAB values of 1.00 each), four isolates had SAB values of 0.80-0.99, indicating that they are
highly similar, but are non-identical, while five isolates are unrelated (SAB lower than 0.80). This suggests that
microevolution might have occurred and that these clinical isolates may possibly belong to different strains.
Conclusion, significance and impact of study: A fair degree of genetic heterogeneity was found among the 14 C.
albicans isolates from UMMC. To our knowledge, this is the first report on the genetic profiles of C. albicans bloodstream
infection isolates from Malaysia, warranting further studies in the possible evolutionary trends within this Candida
species in Malaysia.
Keywords: Randomly Amplified Polymorphic DNA PCR (RAPD-PCR), Candida albicans, Candida bloodstream
infections, Genetic relatedness, DNA fingerprinting
Candida albicans
2.In vitro growth of candida albicans on several resilient dinture liners.
Chae Heon CHUNG ; Kwang Won KIM ; Dong Ki KIM ; Zang Hee LEE
The Journal of Korean Academy of Prosthodontics 1993;31(1):19-27
No abstract available.
Candida albicans*
;
Candida*
3.Ultrastructure of Fibrillar Layer of Candida albicans in Serum Culture.
Joo Young PARK ; Choon Myung KOH ; Woon Seob SHIN ; Dong Hwa KIM ; Kyoung Ho LEE ; Kyung Hoon KIM ; Yoon Sun PARK
Journal of Bacteriology and Virology 2001;31(2):105-112
No abstract available.
Candida albicans*
;
Candida*
4.Molecular characterization of genomic DNA and antifungal susceptibility of candida albicans.
Journal of the Korean Society for Microbiology 1992;27(2):173-180
No abstract available.
Candida albicans*
;
Candida*
;
DNA*
5.Evaluation of Spectrophotometric Broth Microdilution Method to Determine the Fluconazole MIC of the Candida Species.
Ji Yon YI ; Jong Hee SHIN ; Kyung Won LEE ; Dong Eun YONG ; Myoung Jong CHAE ; Soon Pal SUH ; Dong Wook RYANG
The Korean Journal of Laboratory Medicine 2002;22(4):253-259
BACKGROUND: Although the broth microdilution method has been recently established for antifungal susceptibility testing of the Candida species, there is still an argue in the interpretation of the trailing endpoint. We evaluated the spectrophotometric broth microdilution method (SBM) to determine the fluconazole MICs from five different Candida species. METHODS: A total of 252 clinical isolates of five Candida species (144 C. albicans, 42 C. tropicalis, 32 C. glabrata, 28 C. parapsilosis, and 6 C. krusei) were tested for fluconazole susceptibility with the broth microdilution method. The MICs were spectrophotometrically determined at 80% (Spec-80%) and 50% (Spec-50%) decrease in absorbance as compared with growth control, respectively. The results were compared with the fluconazole MICs tested by the National Committee for the Clinical Laboratory Standards (NCCLS) macrodilution method. RESULTS: When MICs were obtained by Spec-80%, the agreements of SBM and the NCCLS macro dilution method within two doubling dilutions were 92.4% (220/238) at 24 h and 78.6% (198/252) at 48 h for all Candida species. Using the Spec-50%, those were increased to 97.9% (233/238) at 24 h and 98.8% (249/252) at 48 h (P<0.01). Especially, for C. albicans and C. tropicalis, the agreement of the Spec-50% was significantly higher than those of the Spec-80% at 48 h; 97.9% vs. 75.0%, for C. albicans (P<0.01), and 100% vs. 57.1%, for C. tropicalis (P<0.01). CONCLUSIONS: These data suggest that the SBM using Spec-50% can provide a more precise and objective mean for fluconazole susceptibility testing, especially for C. albicans and C. tropicalis.
Candida albicans
;
Candida*
;
Fluconazole*
6.Evaluation of aniline blue dye method for rapid identification of candida albicans.
Kap Jun YOON ; Won Keun SONG ; In Ho JANG ; Dong Hun SHIN
Korean Journal of Clinical Pathology 1993;13(2):271-274
No abstract available.
Candida albicans*
;
Candida*
7.First Case of Echinocandin-Resistant Candida albicans in Korea.
Min Seung PARK ; Jong Eun PARK ; Dong Joon SONG ; Hee Jae HUH ; Silvia PARK ; Cheol In KANG ; Jong Hee SHIN ; Nam Yong LEE
Annals of Laboratory Medicine 2017;37(6):556-558
No abstract available.
Candida albicans*
;
Candida*
;
Korea*
8.Determination of antifungal ability of denture cleansing agents to candida albicans.
Sang Sup CHUN ; Chae Heon CHUNG ; Zang Hee LEE
The Journal of Korean Academy of Prosthodontics 1993;31(1):28-38
No abstract available.
Candida albicans*
;
Candida*
;
Dentures*
;
Detergents*
9.Studies on sensitivity and synergism of antifungal agents against candida albicans.
Hae Chull NAH ; Seung Chul LEE ; Inn Ki CHUN ; Young Pio KIM
Korean Journal of Dermatology 1991;29(4):491-499
No abstract available.
Antifungal Agents*
;
Candida albicans*
;
Candida*
10.Genes Expression of Human Monocytes in Response to Candida albicans by Microarray and Its Clinical Application.
Korean Journal of Perinatology 2006;17(1):19-24
No abstract available.
Candida albicans*
;
Candida*
;
Humans*
;
Monocytes*