1.Molecular epidemiology study on an acute gastroenteritis outbreak caused by a new GII. 4 norovirus recombinant strain.
Can-lei SONG ; Jia-chun YUAN ; Zhi-cheng CAO ; Lan LI ; Hai-ming ZHONG ; Jian-ming ZHU ; Xia GAO
Chinese Journal of Preventive Medicine 2013;47(12):1114-1117
OBJECTIVETo analyze the epidemiological characteristics of an acute gastroenteritis outbreak in a nursing home caused by norovirus (NoV) and the genotype of the pathogen.
METHODSOn January 29th 2013, a total of 26 acute gastroenteritis patients infected by norovirus were reported in the nursing home of Jinshan, Shanghai. A questionnaire was used to acquire information of patients involved in the outbreak, 9 stool or anal swab samples were collected from 9 patients without treatment by simple random sampling method, and 4 environmental samples from the surface of doorknobs or toilets were collected. The samples were detected by Real-time PCR for NoV, and positive samples were then amplified by routine RT-PCR. The PCR products were purified, sequenced, and aligned by comparing sequences in GenBank. Phylogenetic trees were constructed by using Clustal X, employing MEGA 5.1 program package.
RESULTSFor the 26 patients, 7 were men and 19 were women.8 samples were found NoV positive among the 13 samples when detected by real-time PCR. The sequence alignment showed that the nucleotide sequence homology between Jinshan08 and Jinshan12 strain which obtained sequencing signal was 100%. The phylogenetic analysis revealed that Jinshan08, Jinshan12 and GII.e/NV2634/BCN/Spain/2008 strains in the RdRp region were on the the same branch of evolutionary tree, the confidence level was 99%, and in the N/S region of the Capsid, 2 other strains and Lordsdal strain were in the same branch, the confidence level was 97%.
CONCLUSIONIt was confirmed that the acute gastroenteritis outbreak was caused by the new GII.4 NoV recombinant.
Aged ; Aged, 80 and over ; China ; epidemiology ; Disease Outbreaks ; Female ; Gastroenteritis ; epidemiology ; genetics ; virology ; Genetic Variation ; Humans ; Male ; Middle Aged ; Molecular Epidemiology ; Norovirus ; classification ; genetics ; isolation & purification ; Phylogeny
2.Epidemiological characterization of sapovirus gastroenteritis aggregation in a kindergarten in Jinshan District, Shanghai
Lin ZHU ; Zhao-peng DONG ; Jian-ru DU ; Ping-hua MO ; Tang WANG ; Can-lei SONG
Shanghai Journal of Preventive Medicine 2021;33(6):521-524
Objective:To analyze the epidemiological characteristics of an aggregational gastroenteritis and determine the genotypes of sapovirus, and to provide scientific basis for formulating effective control strategies. Methods:Unified case definition, active case search and descriptive epidemiology were used to analyze the epidemic. Feces or anal swabs of untreated students, teachers, canteen staff as well as canteen environment samples were collected. Norovirus and sapovirus nucleic acid tests were conducted by real-time fluorescent RT-PCR, and sapovirus nucleic acid was amplified by conventional RT-PCR. The gene region of capsid protein was analyzed by MEGA7.0 software and phylogenetic tree was constructed. Results:A total of 12 cases were reported in the epidemic, and the incidence rate was 44.44%. All reported cases, with vomiting symptoms, were found in the same class. The epidemic showed a point-based outbreak. The first case became the source of infection in class, and the epidemic lasted for 8 days. Real-time fluorescent RT-PCR assay confirmed that five children's feces were positive for sapovirus nucleic acid, and the first-episode children's feces were positive for sapovirus and GII norovirus nucleic acid. Sequence alignment result showed that the sapovirus strains belonged to GI.1 type with homologous genes. Conclusion:Based on the clinical manifestations, field epidemiological investigation and laboratory test results, we confirm that the first case of the epidemic in class is caused by GI.1 sapovirus infection. The epidemic is effectively controlled by comprehensive measures such as case isolation and disinfection.
3.Hybrid procedure for infants/children treatment with pulmonary stenosis under transesophageal echocardiographic guidance.
Li XIE ; Can HUANG ; Sijie WU ; Wancun JIN ; Lei GAO ; Qin WU ; Jinfu YANG ; Zhongshi WU ; Tianli ZHAO ; Yifeng YANG ; Fenglin SONG
Journal of Central South University(Medical Sciences) 2016;41(7):691-695
OBJECTIVE:
To investigate the outcomes of hybrid procedure in treating 10 infants/children with pulmonary stenosis under transesophageal echocardiographic guidance.
METHODS:
Between September, 2009 and December, 2015, 10 infants/children underwent hybrid procedure of transthoracic balloon pulmonary valvuloplasty for pulmonary stenosis in the Second Xiangya Hospital, Central South University. The age, height and weight at the time of admission were 0.7-42 (14.8±15.8) months, 53-97 (74.8±16.3) cm, and 4-15.5 (9.3±4.1) kg, respectively. Atrial septal defect, patent foramen ovale, patent ductus arteriosus, muscular ventricular septal defect, persistent left superior vena cava and tricuspid regurgitation were found in 2, 6, 1, 2, 1 and 5 cases, respectively.
RESULTS:
After the operation, all patients were sent into ICU. The mean duration mechanical ventilation, ICU stay and hospitalization were 0.5-41(6.8±12.3) h, 2-85 (31.1±22.8) h, and 6-20 (11.4±5.1) d, respectively. Postoperative transvalvular pressure gradient reduced to 16-45 (31.1±9.8) mmHg, which was decreased significantly compared with that in preoperative (P<0.001). There was no death during hospitalization and follow-up.
CONCLUSION
Hybrid procedure of transthoracic balloon pulmonary valvuloplasty for pulmonary stenosis under transesophageal echocardiographic guidance is a safe and effective treatment.
Child
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Echocardiography, Transesophageal
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Heart Septal Defects, Atrial
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Heart Septal Defects, Ventricular
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Humans
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Infant
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Pulmonary Valve Stenosis
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Treatment Outcome
4. Clinicopathological characteristics of type 2 diabetes mellitus complicated with colorectal cancer
Zihan HAN ; Jiajia CHEN ; Nan FENG ; Pengfei NIU ; Can SONG ; Zhaoya GAO ; Qingkun GAO ; Dengbo JI ; Wenbo WANG ; Fuming LEI ; Jin GU
Chinese Journal of Gastrointestinal Surgery 2019;22(10):966-971
Objective:
To analyze the clinicopathological features of type 2 diabetes mellitus complicated with colorectal cancer (DCRC).
Methods:
A case-control study was conducted. Inclusion criteria: (1) hospitalized patients receiving fibrocolonoscopy; (2) adenocarcinoma and mucinous adenocarcinoma diagnosed by pathology; (3) with preoperative cTNM clinical staging; (4) colorectal cancer patients undergoing surgical treatment; (5) with postoperative pTNM staging; (6) no smoking or drinking habits. Exclusion criteria: (1) familial adenomatous polyposis (FAP); (2) Lynch syndrome; (3) carcinoma of anal canal and perianal carcinoma; (4) multiple primary cancer; (5) with serious cardiocerebrovascular diseases or multiple organ failure. Clinicopathlogical data of 32 DCRC patients who were diagnosed and treated in Peking University Shougang Hospital from December 2017 to December 2018 were retrospectively collected and analyzed. Forty nondiabetic colorectal cancer (CRC) patients during the same period were selected as control group according to the sex ratio and the age difference less than 5 years. Student′s