1.Study on the relationship between mitochondrial gene mutation and latent autoimmune diabetes mellitus in adults.
Can CUI ; Qiang LI ; Yina ZHANG ; Jinchao ZHANG
Chinese Medical Sciences Journal 2002;17(2):73-76
OBJECTIVETo identify the A3243G mutation of mitochondrial (mt) DNA in patients with latent autoimmune diabetes mellitus in adults (LADA) of Han nationality in the northeast area of China.
METHODSSeventy-nine diabetics of Han nationality, whose families have resided in the northeast area of China for more than 3 generations, were divided into 3 groups: Group 1 (22 cases of type 2 diabetes with maternal inheritance history), Group 2 (34 cases of LADA), Group 3 (23 cases of type 1 diabetes in adolescents). The A3243G of mt DNA was detected in these 79 subjects with the method of PCR-RFLP.
RESULTSNone of the 79 diabetics studied was positively identified for the A3243G mutation of mt DNA.
CONCLUSIONThe A3243G mutation of mt DNA might not be related to the onset of LADA in diabetic population of Han nationality in northeast area of China and there might not be close relationship between A3243G mutation of mt DNA and autoimmunity.
Adolescent ; Adult ; Autoimmune Diseases ; genetics ; DNA, Mitochondrial ; genetics ; Diabetes Mellitus, Type 1 ; genetics ; Diabetes Mellitus, Type 2 ; genetics ; Female ; Humans ; Male ; Middle Aged ; Point Mutation
2.Intestinal Function Changes in Mice after Spinal Cord Injury in Early Stage
Yu LIANG ; Can LI ; Lan LUO ; Zheng YANG ; Qiang WANG ; Jun MA ; Yangyang WANG ; Lulu ZHANG ; Wei YANG ; Fang YI
Chinese Journal of Rehabilitation Theory and Practice 2015;21(2):148-152
Objective To investigate the influence of spinal cord injury on intestinal function in mice after a short period. Methods 105 Kunming mice were randomly assigned to normal group (group A, n=30), sham group (group B, n=30) and model group (group C, n=45). Group A received no treatment, group B was only exposed the spinal cord, while group C was induced by a constant compression with a modified arterial forcep at T10 to establish spinal cord injury model. 12 hours, 24 hours and 48 hours after modeling, myoelectric slow wave activity and tension activity of the ileum were tested, and HE staining was used. Results Compared with groups A and B, the slow wave activity was significantly weaker in group C at every time point (P<0.05), so was the amplitude of tension activity (P<0.05). Frequency of tension activity was obviously higher in group C than in groups A and B 24 hours and 48 hours after modeling (P<0.05). The injury scores were higher in group C than in groups A and B (P<0.05). Conclusion There is reduce in myoelectric slow wave activity, tension activity of the ileum and mild injury in intestinal mucosa in mice after spinal cord injury in the early stage.
3.Expressions of cyclooxygenase-2 and vascular endothelial growth factor in human lung cancer tissue.
Xiaofei LI ; Can LI ; Wei ZHANG ; Kunxiang GAO ; Yifang ZHU ; Qiang LU
Chinese Journal of Lung Cancer 2005;8(2):112-115
BACKGROUNDElevated expression of cyclooxygenase-2 (COX-2) has been found to be a feature of tumors recently. In this study, the expression and significance of COX-2 and vascular endothelial growth factor (VEGF) were explored in the cancer tissues of patients with lung cancer.
METHODSExpression of COX-2 and VEGF in cancer tissues of 126 lung cancer patients were detected by tissue chip and immunohistochemical methods. The relationships among COX-2 expression, survival time and the biology beha- vior of lung cancer were analyzed by retrospective cohort study.
RESULTSExpression of COX-2 was positive in 86 cases (68.25%). The correlation between COX-2 expression and some biology behaviors of lung cancer (T stage, N stage and P-TNM stage) was significant in statistics (P < 0.05). High expression of VEGF was also found in cancer tissues (98/126, 77.78%). The correlation between COX-2 expression and VEGF expression was significant in statistics (Chi-Square=6.72, P < 0.05). The survival time of patients with positive COX-2 expression and VEGF expression was significantly shorter than that without expression (P < 0.05).
CONCLUSIONSHigh COX-2 and VEGF expressions are detected in lung cancer tissue. Each of them is suggested to be an independent predictor of survival in survival analysis. COX-2 and VEGF are significant poor prognostic factors in the surgically resected lung cancer.
4.Effect of tiaozhi yanggan decoction in treating patients with non-alcoholic fatty liver.
Can-li GU ; Yun-ke ZHANG ; Yue-xiao FU ; Shao-feng YANG ; Xin-qiang LI
Chinese journal of integrative medicine 2007;13(4):275-279
OBJECTIVETo observe the clinical efficacy and safety of Tiaozhi Yanggan Decoction (TZYGD) in treating non-alcoholic fatty liver.
METHODSOne hundred and thirty-eight patients were enrolled and randomized into two groups according to the random number table in a ratio of 3:1, with 8 cases eventually dropping out. The symptoms, signs, liver function markers, blood lipids, iconographic indices and clinical comprehensive efficacy after a 12-week treatment course were assessed in 101 patients treated with TZYGD in the treated group and 29 patients treated with Thiola in the control group.
RESULTSThe total effective rate in the treated group and the control group was 81.19% and 68.97%, respectively, showing a significant difference between the two groups with the former being significantly higher than the latter (P<0.05). Moreover, the improvements in the symptoms, signs, liver function, blood lipids and iconographic indices in the treated group were favorable with no serious adverse reactions.
CONCLUSIONTZYGD is effective and highly safe in treating non-alcoholic fatty liver.
Adult ; Body Mass Index ; Body Weight ; drug effects ; Drugs, Chinese Herbal ; adverse effects ; therapeutic use ; Fatty Liver ; blood ; drug therapy ; physiopathology ; Female ; Humans ; Lipids ; blood ; Liver ; physiopathology ; Male ; Medicine, Chinese Traditional ; adverse effects ; methods ; Middle Aged ; Phytotherapy ; adverse effects ; Treatment Outcome
5.COVID-19 rehabilitation awareness among medical staff: Results of a knowledge, attitudes, and practices survey
Zhengliang LI ; Qiang HUA ; Chanjuan ZHENG ; Can DUAN ; Qingling LI ; Wenguang XIA
Chinese Journal of Physical Medicine and Rehabilitation 2020;42(10):919-923
Objective:To investigate the knowledge, attitudes and practices of medical staff rehabilitating COVID-19 survivors to provide references for related training.Methods:A questionnaire survey was administered in early March 2020 to 296 medical staff assessing their knowledge, attitudes, and practices in COVID-19 rehabilitation.Results:More than half of those surveyed received full marks on knowledge and attitudes, but only about 43% received full marks for their practices. Significant differences were found in the knowledge, attitude and practices scores among medical staff of different ages, with different numbers of years of training and working experience, with different professional titles, and in different departments.Conclusion:The COVID-19 rehabilitation knowledge and practices of a minority of the medical staff tested needed improving, and targeted training should be conducted.
6.The accuracy of real-time continuous monitoring system at different stages and its association with glucose excursion
Yingying WANG ; Qiang LI ; Ping YU ; Ping GAO ; Yanhui WU ; Can CUI ; Wei WANG ; Lili CHEN ; Hong QIAO ; Lihong WANG ; Ying XIANG ; Zhong ZHANG ; Jinchao ZHANG
Chinese Journal of Endocrinology and Metabolism 2011;27(3):224-228
Objective To study the accuracy of real-time continuous monitoring system (RT-CGMS) at different stages and its association with glucose excursion. Methods Totally 33 patients with type 1 diabetes or type 2diabetes were under surveillance of RT-CGMS for 5 d. Capillary glucose values were measured 7 times daily.Correlation coefficient, error grid analysis (EGA), and Bland-Altman analysis methods were used to assess the correlation, accuracy and agreement of RT-CGMS at different stages and in general level; The mean amplitude of glucose excursion (MAGE) and the frequency of glucose excursion ( FGE ) were also calculated. Results ( 1 ) The correlation coefficient of RT-CGMS with capillary glucose values at fasting, postprandial stages, and in general level were 0.94,0.92, and 0.93 respectively( P<0.01 ). (2) EGA showed that 98.82%, 98.39%, and 98.64% of the results fell in the A and B zones and 1. 18%, 1.61%, and 1.36% fell in the D zone respectively at fasting,postprandial stages, and in general level. There is no result fell in C and E zones. ( 3 ) The agreement analysis showed that RT-CGMS readings were in close agreement with capillary glucose values at fasting, postprandial periods, and in general level. (4)The MAGE at fasting, postprandial periods, and in general level were (3.57±2.66), (4.07±3.09), and (4. 02 ±3.04) mmol/L (P>0. 05), (0±0. 5), (3± 1), and( 1 ±3) d for FGE (P<0. 01 ).Conclusion RT-CGMS at fasting stage has higher accuracy than postprandial stage and general level, FGE at fasting stage is higher than postprandial stage and general level.
7.Antibacterial effect of niaoluqing oral liquid on clinical drug-resistant strains and different serotype strains of Ureaplasma urealyticum in vitro.
Yuan LU ; Da-Can CHEN ; Qiang LI ; Guo-Wei XUAN ; Bao-Jian FAN ; Ji-Wen ZHAO ; Ning WANG
National Journal of Andrology 2002;8(2):152-154
OBJECTIVESTo study the antibacterial effect of Niaoluqing Oral Liquid (NOL) on clinical drug-resistant strains and 14 serotype strains Ureaplasma Urealyticum (UU).
METHODSSixty-three clinical strains of UU were detected to determine their serology and antibiotic susceptibilities by the metabolic inhibition test (MIT). Mininum inhibitory concentration (MIC) was used to evaluate the sensitivity of NOL to different serotypes of UU. The sensitivity of NOL, erythromycin and tetracycline to 63 clinical strains of UU was also studied.
RESULTSIn 63 clinical strains of UU, the range of MIC to NOL was from 0.48 mg/ml to 15.63 mg/ml, MIC50 < or = 1.95 mg/ml, MIC90 < or = 3.91 mg/ml. Among them, 31 strains were resistant to tetracycline and 31 were resistant to erythromycin. No obvious correlation between the sensitivity of NOL to UU clinical strains and that of erythromycin and tetracycline to UU clinical strains (P > 0.05). Clinical strains of UU in this experiment contains all of its serotypes, also having a higher sensitivity to NOL (MIC < or = 3.91 mg/ml) except serology 1, 2, 3 and 11 (MIC > or = 7.81 mg/ml).
CONCLUSIONSNOL exerts a strong in vitro antibacterial effect on erythromycin-resistant and tetracycline-resistant clinical strains of UU. All kinds of serotype strains had a higher sensitivity to NOL, too. Chinese medicinal herbs are of momentous significance in the treatment of UU infection.
Anti-Bacterial Agents ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Microbial Sensitivity Tests ; Ureaplasma urealyticum ; drug effects ; isolation & purification
8.Identification and analysis of Corydalis boweri, Meconopsis horridula and their close related species of the same genus by using ITS2 DNA barcode.
Rong-kun DOU ; Zhen-fei BI ; Rui-xue BAI ; Yao-yao REN ; Rui TAN ; Liang-ke SONG ; Di-qiang LI ; Can-quan MAO
China Journal of Chinese Materia Medica 2015;40(8):1453-1458
The study is aimed to ensure the quality and safety of medicinal plants by using ITS2 DNA barcode technology to identify Corydalis boweri, Meconopsis horridula and their close related species. The DNA of 13 herb samples including C. boweri and M. horridula from Lhasa of Tibet was extracted, ITS PCR were amplified and sequenced. Both assembled and web downloaded 71 ITS2 sequences were removed of 5. 8S and 28S. Multiple sequence alignment was completed and the intraspecific and interspecific genetic distances were calculated by MEGA 5.0, while the neighbor-joining phylogenetic trees were constructed. We also predicted the ITS2 secondary structure of C. boweri, M. horridula and their close related species. The results showed that ITS2 as DNA barcode was able to identify C. boweri, M. horridula as well as well as their close related species effectively. The established based on ITS2 barcode method provides the regular and safe detection technology for identification of C. boweri, M. horridula and their close related species, adulterants and counterfeits, in order to ensure their quality control, safe medication, reasonable development and utilization.
Base Sequence
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China
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Corydalis
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chemistry
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classification
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genetics
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DNA Barcoding, Taxonomic
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methods
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DNA, Plant
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chemistry
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genetics
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DNA, Ribosomal Spacer
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chemistry
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genetics
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Molecular Sequence Data
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Nucleic Acid Conformation
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Papaveraceae
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chemistry
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classification
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genetics
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Phylogeny
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Plants, Medicinal
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chemistry
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classification
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genetics
9.Construction of Saccharomyces cerevisiae Mutant Deficient in adh2 and ald6 Genes
Yan-Zun WANG ; Juan-Juan LEI ; Xian-Zhang JIANG ; Yuan-Yuan GAO ; Xin LI ; Can-Hua LAN ; You-Qiang CHEN ; Ru-Kai CHEN ; Jian-Zhong HUANG
Microbiology 1992;0(02):-
The purpose of this investigation is to improve ethanol production and decrease acetate formation in Saccharomyces cerevisiae strain YS2-?adh2.The strain YS2-?adh2 with deleted alcohol dehydrogenase Ⅱ(adh2) gene was isolated in our lab with higher ethanol production than that of the strain YS2.The ace-taldehyde dehydrogenase Ⅵ(ald6) gene encoded a cytosolic acetaldehyde dehydrogenase,a key enzyme of the pyruvate dehydrogenase(PDH) bypass,transfers acetaldehyde to acetate.To disrupt ald6 gene of the strain YS2-?adh2,ald6 gene targeting cassettes were synthesized by long flanking homology PCR(LFH-PCR) and then were transformed into YS2-?adh2 mutants by LiAc/SS Carrier DNA/PEG method.Positive transformants were selected with G418 and further confirmed by PCR.Once correctly integrated into the genome,the selective marker was rescued by transforming the plasmid pSH65 into the positive transformants and inducing the Cre expression with a Cre/loxP-mediated marker removal procedure.We named the ald6 gene knocked-out strain as YS2-?adh2-?ald6 which has a 12.5% higher ethanol production and a 18% lower acetate formation compared to the strain YS2.
10.Preparation of a 96-microwell plate DNA diagnostic chip for detection of foodborne bacteria and its application in an incident of food poisoning.
Qiu-Hua MO ; Qiang LI ; Ji-Can LIN ; Hua TAN ; Cheng-ning TU ; Li-qing YE ; Zhi-Ming LIU ; Jian DU ; Hong SUN ; Shu-xiang LI ; Sino WANG ; Ze YANG
Journal of Southern Medical University 2010;30(3):417-421
OBJECTIVETo develop a 96-microwell plate DNA diagnostic chip for simultaneous detection of 9 major foodborne bacteria.
METHODSType-specific PCR primers labeled with biotin and oligonucleotide probes were designed according to the conservative genes of 9 major foodborne bacteria Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7 (Stx1 and Stx2), Shigella spp., Listeria monocytogenes, Bacillus cereus, Yersinia enterocolitica, Vibrio cholerae and Vibrio parahaemolyticus. A one-tube multiplex PCR system for simultaneous amplification of these bacteria was established, and the DNA probes were spotted and immobilized in the wells of the plate in 5x5 array format. Stable hybridization system between PCR products and oligonucleotide probes in the microwell was established after condition optimization. Alkaline phosphatase-conjugated streptavidin and NBT/BCIP were used to detect the hybridized PCR products.
RESULTSTwenty standard bacteria strains were used to validate the 96 microwell plate DNA diagnostic chip and highly specific and stable experiment results were obtained. Using this chip assay, the causal pathogen Staphylococcus aureus was identified within 12 h after the sampling from an incident of food poisoning, and the result was consistent with that obtained using conventional bacterial culture and biochemical identification.
CONCLUSIONThe novel 96 microwell plate DNA diagnostic chip allows rapid, accurate, automated and high-throughput bacterial detection and is especially valuable for quick response to such public health emergencies as food poisoning.
Bacteria ; classification ; genetics ; isolation & purification ; DNA, Bacterial ; analysis ; Food Contamination ; analysis ; Food Microbiology ; methods ; Foodborne Diseases ; microbiology ; Humans ; Oligonucleotide Array Sequence Analysis ; methods