1.The current situation and problem of mental health and social support on clinical and English undergraduates of Chongqing medical university
Chongqing Medicine 2014;(9):1113-1114,1116
Objective To understand the status and existing problems of psychological health and social support of medical students ,analyze its influencing factors and provide basis for targeted education and management measures .Methods The social support scale(SSRS) ,symptom self-assessment scale (SCL-90) ,the quality of life questionnaire (SF -36)were used as question-naire survey for 120 college students .Results Positive rate of psychological problems in 91 undergraduates was 24 .18% ,obsessive compulsive disorder appeared the most ,phobia appeared the least .Positive rate of psychological problems in 29 English major un-dergraduates was 41 .38% ,the most frequent symptom was forcing symptoms ,followed by human and depressive symptoms .The interpersonal score of English major undergraduates was higher than that of clinical undergraduates ,with a statistically significant difference (P=0 .039) .Mental health between medical and English major students had a statistical difference (P=0 .010) .Objec-tive support scores between medical and English major students had a statistical significance (P=0 .014) .The highest score was the subjective support ,and the lowest scores was the use of social support .Conclusion The mental health of most of the medical un-dergraduates is not optimistic and there exist different psychological problems .At the same time ,the lower level of social support comes along with the higher level of health problems and vice versa .Therefore ,we suggest that society ,schools ,families and relat-ed management departments should further carry out mental health intervention for medical students .
2.The cagG gene expression of Helicobacter pylori in various digestive diseases and its clinical signifi-cance
Can XU ; Zhaoshen LI ; Zhenxing TU
Chinese Journal of Digestive Endoscopy 1996;0(06):-
0. 05 ) . Conclusion The cagG gene of Hp was quite conservative, most of the Hp strains in Chinese patients were cagG positive, it has no specific predisposition to different disorders and shows no significant correlation to the extent of gastric mucosal inflammation. Therefore cagG can' t be served solely as a related pathogenic gene for certain diseases.
3.Expression of DOG1 protein in gastrointestinal stromal tumors and the relationship between them
Liying ZHEN ; Yan LI ; Can XU
Chinese Journal of Digestion 2010;30(9):585-587
Objective To analyze the association of DOG1 protein with gastrointestinal stromal tumors(GIST). Methods Seventy-seven patients diagnosed with GIST between January 2005 and June 2010 were enrolled. The DOG1 expression in GIST was determined using immunohistochemistry.The result was compared with that of CD117 and CD34. Results Of 77 GIST patients, the DOG1 was expressed in 74 (96.10%) patients and CD117 in 70 (90. 9%) patients, with no significant difference between DOG1 and CD117 (P>0.05). DOG1 expression was not related to the risk stratification of the tumor, site, age and gender (P>0. 05). Of 7 GIST patients with negative CD117,6 patients were positive for DOG1 expression. Whereas 2 of 3 DOG1 negative patients were positive for CD117 expression. Conclusions DOG1, as a novel marker for GIST,has high sensitivity as CD117. It is a good complementarity in diagnosis of GIST when combine DOG1 with CD117, especially in diagnosis of CD117 negative patients. However, the DOG1 can not be used as an index in eveluating the risk stratification of the GIST.
4.Application of Endoscopic Ultrasonography in Diagnosis and Treatment of Early Esophageal Cancer
Yuting LIU ; Can XU ; Zhaoshen LI
Chinese Journal of Gastroenterology 2015;(8):496-499
Early esophageal cancer is derived from mucosa and submucosa without lymph node metastasis. They are detected generally during endoscopy or physical examination without clinical symptoms. Main treatment of early esophageal cancer is endoscopic resection,and the 5-year survival rate is over 90% . Once symptoms occur,local lymph node metastasis is often present and the prognosis is poor with a markedly reduced 5-year survival rate. Thus it is vital to diagnose and treat esophageal cancer at an early stage. With the development of endoscopic techniques,especially the proficiency of endoscopic ultrasonography(EUS),the detection rate of early esophageal cancer has been increased greatly. EUS also plays an important role in the staging of early esophageal cancer. This article reviewed the application of EUS in diagnosis and treatment of early esophageal cancer.
5.Construction of a recombinant attenuated Salmonella typhimurium nucliec acid vaccine carrying Helicobacter pylori HpaA and detecton of its immunogenicity
Can XU ; Zhaoshen LI ; Zhenxing TU
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To construct a recombinant live attenuated Salmonella typhimurium nucleic acid vaccine carrying Helicobacter pylori ( H. pylori ) hpaA gene and to detect its immunogenicity. Methods hpaA gene fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of the standard H. pylori strain and cloned into pUCmT vector. DNA sequence of the amplified hpaA gene was assayed,then cloned into the eukaryotic expression vector pIRES through enzyme digestion and ligation reactions. The recombinant plasmid was used to transform Escherichia coli DH5?,and the positive clones were screened with PCR reaction and restriction enzyme digestion. Then,the recombinant pIRES-hpaA was transformed to LB5000,then the recombinant plasmid was transformed to SL7207,and the recombinant strain was passaged repeatedly. Finally,recombinant pIRES-hpaA was transfected to COS-7 cells with Lipofectamine TM 2000. The immunogenicity of expressed hpaA protein was determined with SDS-PAGE Western blot. Results The hpaA gene fragment of the 750 base pair was amplified from the genomic DNA,and it was consistent with the sequence of the H. pylori hpaA by sequence analysis. It was confirmed by PCR and restriction enzyme digestion that Helicobacter pylori hpaA gene was inserted into the eukaryotic expression vector pIRES and a stable recombinant live attenuated Salmonella typhimurium nucleic acid vaccine carrying Helicobacter pylori hpaA gene was successfully constructed,and specific strip of hpaA expressed by pIRES-hpaA could be detected with Western blotting. Conclusion The recombinant attenuated Salmonella typhimurium nucleic acid vaccine strain expressing HpaA protein with immunogenicity was constructed,it might be helpful for further investigation concerning the immune action of the nucleic acid vaccine in vivo .
6.Evaluation of PSP combined with chemotherapy in the treatment of malignant tumor patients
Sihan LAI ; Li YIN ; Wenwen LI ; Can XU ; Yixi BAO
Chinese Journal of Immunology 2010;26(1):51-56,65
Objective: To evaluate the quality of life,peripheral blood and immune function of patients with malignant tumors treated by PSP.Methods: We searched MEDLINE,Pubmed and other databases by computer.We assessed the identified studies in order to include high quality studies.Results:A total of 7 into the study,701 cases of patients.Meta analysis showed that polysacchariopeptide treatment for patients with certain malignant tumors after chemotherapy might improve the quality of life.Polysacchariopeptide group combined with chemotherapy drugs might improve the white blood cells,hemoglobin,platelet compared to chemotherapy drugs or batyl alcohol drugs combined with chemotherapy.Polysacchariopeptide drug combined with chemotherapy may increase the CD_3~+ ,CD_4~+ ,CD_8~+ and CD_4~+/CD_8~+ ratio,and the NK cells was no significant difference.Conclusion:PSP combined with chemotherapy to treat malignant tumors has a certain effect.However,most of trials included in the review may be of low quality,therefore,it may be neccessity to conduct multi-center randomized-controlled trials of high quality.
7.Research progress of bone microarchitecture and microdamage detection
Can XU ; Mingqing LI ; Chenggong WANG ; Kanghua LI ; Hua LIU
Chinese Journal of Tissue Engineering Research 2016;20(44):6673-6681
BACKGROUND:Bone fragility and poor bone quality due to osteoporosis are a major and increasing concern. Bone microarchitecture and microdamage, the important factors of bone quality, their detection technology and instrument have experienced a long development process. OBJECTIVE:To give a brief introduction of the concept of the bone microarchitecture and microdamage, then to summarize the research progress of their detective methods. METHODS:PubMed and CNKI databases were retrieved for reviews and articles related to bone microarchitecture and microdamage published from January 1990 to June 2016 using the keywords of“bone microarchitecture, bone microdamage and detect/detective/detecting”in Chinese and English, respectively. Finaly a total of 65 articles were selected for overview. RESULTS AND CONCLUISON:(1) Bulk staining is a quick and useful way to confirm and assess linear microcracks and diffuse damage. Micro-CT and confocal microscopy al ow visualization at the micron scale, and are useful tools to understand the three-dimentional nature of bone microdamage. Scanning electron microscope lacks the ability to investigate large regions of microdamage, but al ows users to probe in extensive details at the nano scale. (2) Ultimately, we recommend the use of multiple imaging modalities according to the experimental needs to obtain useful information about bone quality and microdamage formation, across the scales of hierarchy in bone.
8.The effects of different therapies and formulas on CRH mRNA expression in Hypothalamus of rats with chronic psychological stress
Can YAN ; Lili WU ; Zhiwei XU ; Li GAO
Chinese Pharmacological Bulletin 2003;0(10):-
Aim The purpose of this study was to investigate mec ha nism of different therapies and formulas regulating chronic psychological stress . Methods The way of chronic bandaging and confining activity space w as adopted to establish chronic psychological stress rat model. The effects of d ifferent therapies and formulas on CRH mRNA expression in Hypothalamus were anal yzed by RT-PCR method. Results There was significant increase of CRH mRNA expression in Hypothalamus of rats with continual 2 wk psychological stres s(P
10.Construction of Helicobacter pylori hpaA nucleic acid vaccine and detection of its immunogenicity
Can XU ; Zhaoshen LI ; Zhenxing TU ; Al ET ;
Chinese Journal of Digestion 2001;0(10):-
Objective To construct nucleic acid vaccine encoding Helicobacter pylori (H. pylori) hpaA gene. Methods The genomic DNA of the standard H. pylori strain 17874 was isolated. hpaA gene fragment was amplified by polymerase chain reaction (PCR) and cloned into pUCmT vector. The sequence of the amplified hpaA gene was assayed, and then cloned into the eukaryotic expression vector pIRES through a series of enzyme digestion and ligation reactions. The recombinant plasmid was transformed into competent Escherichia coli cells DH5?, and the positive clones were screened by PCR reaction and restriction enzyme digestion . Recombinant pIRES hpaA was transfected into COS 7 cells using Lipofectamine TM 2000, and the immunogenicity of expressed HpaA protein was detected with SDS PAGE and Western blot. Results The 750 base pair hpaA gene fragment was amplified from the genomic DNA and was consistant with the sequence of the H. pylori hpaA by sequence analysis. PCR and restriction enzyme digestion results revealed that the H. pylori hpaA gene was inserted into the eukaryotic expression vector pIRES, suggesting the nucleic acid vaccine pIRES hpaA was successfully constructed. The specific protein strip of HpaA expressed by pIRES hpaA was detected with Western blot. Conclusion The nucleic acid vaccine pIRES hpaA was successfully constructed which may help the further investigation towards the immune action of the nucleic acid vaccine.