Radioactive 125I interstitial brachytherapy is easy to cause the intestinal tract tissue damage.Its mechanism possibly correlates to the quick renewal speed of the intestinal tract tissue and a majority of ceils in M, G2 and late S phases. The tolerant dose of intestinal tract tissue is approximately 160 Gy. If the absorbed dose is higher than the tolerant one, the intestinal tract tissue will be damaged. In the clinical practice of radio-active 125I interstitial brachytherapy, through the strict plan of the TPS system before the operation, by using ul-trasound, CT, MR/and other imaging technologies in the operating process and establishing the suitable barrier protection between the seeds and intestinal tract tissue, the radioactive damage of the intestinal tract tissue my be reduced.

Aim To investigate the effect taurine on ischemia-induced neuron apoptosis and its mechanisms. Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO). After 6 h permanent MCAO, mRNA expressions of Caspase-3/8 of rat brain were detected with real-time quantitative reverse transcriptase-polymerase chain reaction (real-time QRT-PCR). Oxygen-glucose deprivation (OGD) was used to induce an in vitro ischemia model. Primarily cultured cortical neurons were exposed to 4 h OGD. Intracellular calcium concentrations([Ca~(2+)]_i) was measured with fluorescent Ca~(2+) sensitive probe fura 2 acetoxylmethyl ester (Fura 2/AM). Neuronal apoptosis was assayed with flow cytometry of FITC-annexinV/propidium iodide binding 24 h after OGD. Results mRNA expressions of Caspase-3/8 were upregulated in MCAO model. [Ca~(2+)]_i and neuronal apoptosis were markedly increased in OGD model. Taurine pretreatment reduced the upregulation of mRNA expression of Caspase-3/8 in vivo and ameliorated calcium overload and neuronal apoptosis in vitro. Conclusion Taurine has neuroprotective effect against ischema-induced neuronal apoptosis. This is partly due to its effects on Caspase-3/8 and [Ca~(2+)]_i

AIM To observe the protective effect of taurine on focal cerebral ischemia in rats. METHODS Male SD rats were divided at random into three groups, i.e. sham-operated, control and treatment group respectively. Before ischemia impairment, taurine(250 mg?kg -1?d -1)was administrated ip for one week in treatment group. A nylon suture was inserted into internal carotid artery to occlude the beginning of middle cerebral artery(MCAO). After 3 h permanent occlusion, neurology deficit score was evaluated. At 6 h, all animals were decapitated rapidly to get brain tissue. Brain infarct region was stained by 2,3,5-triphenyltetrazolium(TTC) and the size was measured by AUTOCAD. Contents of malondialdehyde(MDA) and nitric oxide synthase(NOS) and activity of superoxide dismetase(SOD) in brain tissue were detected by spectrophotometer. Expressions of iNOS and inter-cellular adhesion molecule-1 (ICAM-1) were observed through immunohistochemistry method. RESULTS Compared with the control group:Taurine can ameliorate neurological deficit score and decrease infarct volume induced by MCAO. Taurine improved the activity of SOD, but did not affect NOS activity in the infarct without affecting MDA content after 6 h MCAO. Taurine decreased the positive expression of ICAM-1 significantly in brain slice. CONCLUSION The results suggest that taurine may reduce expression of ICAM-1 and improve activity of SOD, and play an neuroprotective effect against middle cerebral artery occulusion.

Objective: To establish the quatity standard of Sedum aizoon L..Methods: The micro-method and TLC were used for qualitative identification,and a HPLC analysis was applied for quantitative determination of quercetin.Results: A qualitative analysis for Sedum aizoon L.was set up,a good linear relationship was obtained over the range of 1.216-2.16?g/ml and regression equation was Y=40386X-14138(r=0.9991).The average recovery rate was 102.08%(RSD=0.92%).Conclusion: The method is simple,accurate and suitable for quality control of Sedum aizoon L.

AIM Taurine was reported neuroprotective under several ischemic models in vivo. In this study, the direct effect of taurine against oxygen-glucose deprivation (OGD) inducing acute neuronal injury and the underlying mechanisms in vitro were investigated. METHODSFour hours OGD was used to induce in vitro ischemic injury in rat cortical neurons. Taurine 5, 10 and 20 mmol·L-1 was added 20 h before and during 4 h OGD period respectively. Mortality rate of neuron was assayed by MTT and flow cytometry methods. Level of neuronal [Ca2+]i was detected by Fura 2/AM loading. Amino acid concentrations in culture media were measured by high performance liquid chromatography. RESULTS Under OGD conditions, neuronal death was markedly increased, and the levels of neuronal [Ca2+]i and extracellular glutamate level were enhanced obviously. Taurine pretreatment obviously decreased the percentage of neuronal death induced by OGD. In addition, abnormal elevation of neuronal [Ca2+]i and extracellular glutamate level induced by OGD both were markedly repressed by taurine. CONCLUSION Taurine can alleviate rat cortical neuron injury induced by OGD, the mechanisms were likely due to repressing calcium overload and inhibiting excessive release or leakage of glutamate under such conditions.

Air ; analysis ; Air Pollutants, Occupational ; analysis ; Humans ; Occupational Exposure ; Phthalic Acids ; analysis ; Spectrophotometry ; methods ; Workplace

Objective To summarize the experience in treating coronary artery fistula (CAF) by using Guglielmi detachable coils. Methods During the period from July 2009 to November 2014 at the Affiliated Changhai Hospital of Second Military Medical University, interventional treatment of CAF by using Guglielmi detachable coils was performed in 40 patients. The clinical data were retrospectively analyzed. The feasibility, safety and effectiveness of this technique were evaluated. Results Successful transcatheter closure of CAF with Guglielmi detachable coils was achieved in all 40 patients; the average Guglielmi detachable coils used in each patient was(2.33±1.38) coils. No procedure-related complications occurred. Intra-operative angiography showed that residual shunt completely disappeared in 12 patients (30%) and blood flow was significantly decreased in 28 patients (70%). All the patients were followed up for 1-65 months, neither complications such as recurrent bleeding and ischemia nor stenosis and occlusion of related arteries, or fistula cavity rupture occurred. Conclusion The use of Guglielmi detachable coil in interventional treatment of CAF is safe and effective, although its long-term effect needs to be further verified.

Objective To explore the chromosome aberration of cervical cancers induced by human papillomavirus(HPV16)virus.Methods The change of change of chromosomes of Hela cells induced by HPV-16 infection HPV-16 in fection was detected by FISH.Results The integration sites of HPV-16 were not observed in Hela cells.But HPV16 virus was integrated on chromosomes 3.Conclusion HPV16 could cause chromosome 3 aberration in Hela cells and result in the occurance of cervical cancers.

Objective To standardize the operation processes of neonatal instrument,ensure the standards effectiveness and safety of equipment use.Methods A retrospective study had been taken on after the developments and implementations of all neonatal instruments standard operation procedure (SOP).The equipment maintenance data were collected and analyzed one year before and after the implementation of SOP.Results After the effective implementation of the SOP,the incidence of instrument repair due to misoperation,improper maintenance reduad by 59％(19/32),9/14,4/7,the instrument management efficiency was enhanced,and the safety of nurses and patients was guaranteed,and the patients satisfaction was improved,and also the quality of care was enhanced.Conclusions SOP procedure plays a very important role in improving neonatal care management and overall efficiency.It makes a continuous improvement of nursing care which is worth widely being implemented.

Objective To investigate the effect of preoperative prophylactic parenteral nutrition on prognosis of advanced gastric cancer.Methods A total of 159 patients with advanced gastric cancer in our hospital were collected,and randomly divided into experimental group and control group.80 cases in experimental group were treated with prophylactic parenteral nutrition,and 79 cases in control group were treated with traditional nutrition.The partial indexes of liver function,complications and mortality rate were compared between the two groups before and after surgery.Results The levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT),total bilirubin (TBIL) and direct bilirubin (DBIL) were much lower in experimental group than in control group at 8 days after surgery (t=6.542,6.460,5.634,5.275,all P＜0.001).The levels of ALB and PAB were much higher in experimental group than in control group at 2 and 8 days after surgery (t=12.580,12.471,6.757,12.821,all P＜0.001).The incidence of complications and mortality rate were much lower in experimental group than in control group (x2 =23.409,4.818,P＜0.001 or 0.05).Conclusions Preoperative prophylactic parenteral nutrition can speed up the recovery of liver function and reduce the complications and mortality rate after surgery in patients with advanced gastric cancer.