1.A Preliminary Study on the Establishment of Animal Model with Hyperuricemia
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Hypoxanthine,the pre_substance of uric acid,was used to create mouse model with hyperuricemia.The dose_effect relationship and time_effect relationship of hypoxanthine during the model establishment were observed.The effect of sex and xanthine oxidase inhibitor on the establishment of mouse model was studied.The results showed this method is effective for the establishment of a stable mouse model with hyperuricemia.
2.Investigation on Sanitation of Cosmetics in Yunnan Province and the Research of Security Countermeasure
Can SUN ; Haiyan DONG ; Ping YANG
Journal of Environment and Health 1989;0(06):-
Objective To know the cosmetics quality in Yunnan Province and to research the security countermeasure in order to protect the the health of people. Methods According to the state,standard test methods and criterion,the physical,chemical,biological and toxicological indicators of cosmetics were determined and evaluated. The results were treated by statistic methods. Results From 1996 to 2005,1 581 cosmetics samples were determined. The eligible rate of the cosmetics samples was 90.1%(1 425/1 581). The eligible rate was increasing from 1996 to 2005(?2=19.487,P
4.Changes in energy metabolism and serum enzyme biomarker under static load in rabbits.
Can WANG ; Su ZHAO ; Cheng-Jun YANG
Chinese Journal of Applied Physiology 2008;24(2):150-165
Animals
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Creatine Kinase
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blood
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Energy Metabolism
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Lactate Dehydrogenases
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blood
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Male
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Pressure
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adverse effects
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Rabbits
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Serum
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enzymology
5.Study Advances in Synergism and Detoxication of Drug Combination with Cisplatin in Chemotherapy
Hong YANG ; Can GONG ; Changhong WANG ; Cong QI
China Pharmacist 2015;(5):836-840
Cisplatin is effective in the treatment of various solid tumors. However, its clinical application is limited because of the severe side-effects such as nephrotoxicity, hepatotoxicity, ototoxicity and so on. Drug combination is advocated in clinics for synergism and detoxication. In order to provide some referencs for the research of drug combination and clinical treatment regimen, the research advances in the synergism and detoxication of drug combination of cisplatin with chemicals/ traditional Chinese medicines in recent years are reviewed in the paper.
6.Use of animal models of atrial fibrillation in the study of cellular and molecular therapy
Jiayuan HUANG ; Jian WU ; Fenghua YANG ; Can CHEN
Acta Laboratorium Animalis Scientia Sinica 2015;(2):205-208
Atrial fibrillation ( AF) is an abnormal heart rhythm characterised by rapid and irregular beating.It is caused by multiple factors and can lead to ischemia-associated thrombosis, heart failure and other complex symptoms. Based on the etiology and characteristics of AF, animal models have 3 main categories including electrical, neurohormonal or vessel-related, and structural remodeling models.New technologies such as microRNA knock-down/overexpression or CRISPR-Cas9 gene editing provide tools for constructing animal AF models and directions in the development of AF thera-peutic strategies.Currently these strategies have largely focused on the cellular and molecular therapeutics rather than tradi-tional invasive electrophysiological methods or antiarrhythmic drugs.With the aid of new tools, progress has been greatly made in a broad range of therapeutic research areas including molecular mechanisms, drug targeting and screening.This re-view summarizes the animal models of atrial fibrillation currently used in studies of the molecular and cellular therapeutics and notes their contributions to this research area.
7.Rosiglitazone upregulates Foxp3 mRNA expression of CD4+T cells in adults with latent autoimmune diabetes
Zhifang YANG ; Zhiguang ZHOU ; Can HUANG ; Jian PENG ; Xiang YAN
Chinese Journal of Microbiology and Immunology 2008;28(3):264-268
Objective To investigate the effect of rosiglitazone on the CD4+regulatory T cells in the patients with latent autoimmune diabetes in adults(LADA).Methods The CIM+T cells from IADA patients were isolated with anti-CD4-dynal magnetic beads.The expression of Foxp3 mRNA,along with peroxisome proliferators activator receptors gamma(PPARγ)mRNA and TGF-131 mRNA was determined.The effect of rosiglitazone on CD4+T cells was measured,after treated with rosiglitazone for 48 h.Cell viability was assessed by Mtit assay.The proliferation was assayed with 3 H-TdR.Two-color staining(anti-CD4,anti-CD25)flow cytometric analysis was employed to measure the percentage of CD4+CD25+T cells of Deriph eral blood.Resuits PPARγmRNA was expressed in peripheral CD4+T lymphocytes.RosiglitazoBe inhibited phytohemagglutinin(PHA)-induced human CD4+T cell proliferation in dose dependence.The percentage of CD4+CD25+T cells showed no significant change after the peripheral blood culture with 1 μmol/Land 10μmot/L rosiglitazone.10 μmol/L of rosiglitazone induced Foxp3 mRNA expression in vitro (3.27fold,P<0.05),whereas TGF-β1 mRNA expression did not change.Furthermore,only 1 μmol/L of rosiglitazone could promote Foxp3 mRNA expression if adding IL-2(10 U/m1)in cultures(3.48 fold.P
8.Conscious analgesia/sedation with remifentanil and propofol on the elderly patients undergoing gastroenterological endoscopic procedure
Erhua WANG ; Dandan GUO ; Can YANG ; Xiaoping GU ; Zhengliang MA
Chinese Journal of Digestive Endoscopy 2016;33(6):384-387
Objective To evaluate the efficacy and safety of the conscious analgesia/sedation scheme with remifentanil and propofol on the elderly patients under gastroenterological endoscopic procedure. Methods Retrospective analysis was performed on 3 500 cases with remifentanil and small?dose propofol under gastroenterological endoscopic procedures, and the procedure achievement, effects of the narcotic, adverse reactions and satisfactory results were summarized. Results All procedures were completed successfully. The ratio of the patients whose intended sedation depth arrived at referential induction was up to 91?3%(3 196/3 500).Hemodynamic change was slight and almost all patients could open eyes in no time, with short hospital stay. No serious adverse effects were observed except several patients complaining about mild bucking, abdominal distension and stomachache. The degree of patients′ satisfaction was high. Conclusion The conscious analgesia/sedation scheme with remifentanil and propofol can be effectively and safely used on gastroenterological endoscopic procedure among the elderly, but precise monitor and timely treatment of complications are still needed.
9.The expression of BP1 mRNA and its clinical significance.
Yang WANG ; Wei-qiang ZHENG ; Can-rong NI
Chinese Journal of Pathology 2005;34(9):602-602
Adult
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Aged
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Aged, 80 and over
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Breast Neoplasms
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metabolism
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pathology
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Carcinoma, Ductal, Breast
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metabolism
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pathology
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Female
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Homeodomain Proteins
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biosynthesis
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genetics
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Humans
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Middle Aged
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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RNA, Messenger
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biosynthesis
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genetics
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Receptors, Estrogen
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metabolism
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Transcription Factors
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biosynthesis
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genetics
10.Pharmacodynamic Study of Yunkang Capsule
Zuoxiao LI ; Shunhan XIAO ; Sijin YANG ; Can TANG ; Shishan ZHANG
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To investigate the pharmacodynamic action of Yunkang Capsule(YKC).Methods The animals were divided into YKC groups(at high-,moderate-and low-dosage respectively),diphenidol control group,model control group and blank control group.The action of YKC on vertigo and thrombosis,hemorrheologic indexes,piamatral microcirculation and free radicals of the animal models were observed.Results YKC could significantly prolong the latency of vertigo,reduce wet weight of thrombus,decrease blood viscosity,plasma viscosity and hematocrit,promote the dilation of piamatral micro-vessels,increase the amount of interweave microdot,increase plasma SOD activity and decline plasma MDA content of animal models.Conclusion YKC has actions of counteracting vertigo and thrombosis,improving hemorrheology and microcirculation,and clearing away free radicals.