1.In Vitro Effect of Fungicides, Plant Extracts and Smoke on Conidial Germination of Fusarium oxysporum Root Rot Pathogen of Piper betle.
Shahidul ALAM ; M Rafiqul ISLAM ; Montaz Ali SARKAR ; Arfatun Nahar CHOWDHURY ; M S ALAM ; Min Woong LEE
Mycobiology 2004;32(1):42-46
Five fungicides such as rovral, bavistin, cupravit, dithane M-45 and thiovit were tested against conidial germination of Fusarium oxysporum. Dithane M-45 was the most effective against the fungus. Rests of the fungicides were more or less effective in the inhibition of conidial germination after 5~30 minutes immersion in 500~2500 ppm concentration. Five plant extracts(leaf extracts of Ocimum sanctum, Lantana camera, Calotropis procera, Azadirachta indica and Vinca rosea) found to be more or less effective against the fungus. 'Dhup' somke have good inhibitory effect on conidial germination of F. oxysporum.
Azadirachta
;
Calotropis
;
Fungi
;
Fusarium*
;
Germination*
;
Immersion
;
Lantana
;
Ocimum
;
Piper betle*
;
Piper*
;
Plant Extracts*
;
Plants*
;
Smoke*
;
Vinca
2.Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower.
M Rowshanul HABIB ; M Rezaul KARIM
Mycobiology 2009;37(1):31-36
A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 microg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 microg/ml for ethyl acetate, 9.19 microg/ml for compound 1 and 15.55 microg/ml for compound 2.
Acetates
;
Artemia
;
Calotropis
;
Chromatography
;
Chromatography, Thin Layer
;
Diffusion
;
Flowers
;
Microbial Sensitivity Tests
;
Phthalic Acids
;
Salts
;
Silica Gel