AIM: To set up the method for adsorbing and separating total flavone in Radix Puerariae and in Folium Crataegi. By macroreticular resin. METHODS: UV Spectrophotometry was used to analyze the content of total flavone in mixture. RESULTS: The D101 was the best for adsorbing and separating total flavone in the following technological conditions: the maximum adsorbing capacity for flavone in Radix Puerariae was 10.10mg?g -1; the maximum adsorbing capacity for flavone in Folium crataegi was 11.28mg?g -1; the current velocity was 3ml?min -1, the eluting reagent 80% ethanol (20 times the volume of medicinal herbs) and the eluting velocity 3ml?min -1. CONCLUSION: D101 resin could be used to refine the total flavone in Radix Puerariae and in the Folium Crataegi.

ve To explore the hygienic safty of cosmetics for special use. Methods The detection and analysis on the hygienic safety of 432 domestic cosmetics for special use saled in Guangdong provincial markets were carried out in 2000. Results The orders of qualified rates of cosmetics for special use from high to low were as fol-lows: deodorant cosmetics (100%), hair-removing cosmetics(88.89% ), breast massage (80.00% ), antisunburn lotion (77.88%), fading cream(75.68% ). hair nourishment(64.29% ), beautifying cosmetics(50.00% ), hair dye (40.74%) and hair pem(28.57%). The highest unqualified rate of hygienic safety test on cosmetics was 17.28% for Ames test, next was 14.81% for chromosome aberration test on mammalian cells in vitro, 14.13% for eye irritation test and 10.88% for skin allergic reaction. Conclusion The management on the hygienic safety of cosmetics for special use should be strengthened further.

Objective To observe the changes in serum High Sensitive C-reactive Protein(hs-CRP)in newly dignosed type 2 diabetic patients after treament with short-term intensive insulin aspart and investigate the relationship of inflammatory factors with insulin resistant.Methods30 newly dignosed ty 2 diabetic were treated by 14 d intensive aspart treament,the levels of fasting plasma glucose(FPG),2hours postprandial glucose(2hPG),insulin and C-peptide,hs-CRP,the mean area under the curve(AUC)of insulin and C-peptide,insulin resistance index(HOMA-A),insulin secretion index(HOMA-B)were compared before and after transient intensive nsulin aspart treament.ResultsAfter the treament,FPG,2hPG,hs-CRP and HOMA-A were significantly decreased(P

Objective: To determine the content of sanguinarine and chelerythrine in Macleaya microcarpa(Maxim) Fedde. Methods: RP-HPLC was adopted, kromasil C 18 column (150mm?4.6mm.5?m) with a mobile phase acetonitrile-0.1%(V/V) phosphoric acid solution(25∶75) by UV detector at 270nm. Results: The average recoveries of chelerythrine and sanguinarine were 90.2%,92.8%,RSD were 1.5%,2.4% respectively. Conclusion: A simple, rapid and sensitive method with good precision was established.

Objective:By measuring the expression of nuclear factor of ?B(NF-?B) and apoptosis in the tissue of benign and malignant pancreatic diseases.Methods:The expression of NF-?B factor was measured by immunohistochemical method to measure.The expression of apoptosis for cells was determined by TUNEL method.Results: When measuring the expression of NF-?B p65 protein in the tissue,we found firstly,in the tissue of pancreatic cancer,NF-?B p65 protein was located in the nucleolus and cytoplasm,the number of positive cell was obviously more than that in the control group(P

Objective To explore the clinicopathelogical features of resectable carcinoma of the head of (pancreas).Methods A retrospcctive analysis was made on the clinicopathological data of 102 patients with cancer of the head of pancreas, who had received pancreatoduodenectomy from Nanfang Hospital in January 1990 to January 2003. Results The incidence rate of the peri-pancreatic tissue infiltration was 74.5%,the infiltration rate of retroperitonealfat was 27.5% and the incidence rate of peri-pancreatic lymph-node (metastasis) was 71.6%. Metastasis rate of 21.1% was seen in the abdominal aorta lymph nodes. (Conclusions) Surgically resectable carcinoma of the pancreatic head is not equivalent to early cancer. The surgical area of radical resection of cancer of the pancreatic head should at least include pancreatoduodenectomy and clearance of regional soft tissue and lymph nodes. It may be more reasonable if the abdominal aorta lymph nodes were assigned to the first station of lymph drainage of carcinoma of head of the pancreas.

Bartter′s syndrome(BS) is a rare renal tubular diseases and an autosomal recessive hereditary disease. The characteristic findings are hypokalemia, metabolic alkalosis, and raised serum renin and aldosterone levels. Combination of metabolic syndrome with Bartter′s syndrome has not been reported so far. Here in, we report a case in order to call attention to the diagnosis and treatment of typical Bartter′s syndrome combined with metabolic syndrome.

Objective To analyze the imaging characteristics and to evaluate the application value of color doppler ultrasonography (CDUS)combined with CT angiography (CTA)and contrast-enhanced magnetic resonance angiography (CE-MRA)in patients with cervical vertigo.Methods 62 patients diagnosed with cervical vertigo clinically were enrolled.Neck CDUS and neck CTA were per-formed on 39 patients.Neck CDUS and neck CE-MRA were performed on 23 patients.Neck CDUS and CTA/MRA were performed on 30 normal volunteers,which were chosen as control group.Neck CDUS and neck CTA were performed on 18 normal volunteers. Neck CDUS and neck CE-MRA were performed on 12 normal volunteers.Hemodynamics and morphology were evaluated and com-pared between the two groups.Results In aspect of morphology:The incidence of vertebral artery (VA)stenosis (46.77%)and VA variation (29.03%)in cervical vertigo group were higher than VA stenosis (23.33%)and VA variation (6.67%)in control group with significant difference (all P <0.05).The incidence of tortuous VA showed no statistics difference between cervical vertigo group (1 1.29%)and control group (13.33%)(P >0.05).In aspect of hemodynamics:The decline incidence of peak systolic velocity de-tected by CDUS in cervical vertigo group (66.13%)was higher than that in control group (10.00%)with statistically significant difference (P <0.05).The peak systolic velocity decline incidence of VA stenosis (86.21% )and VA variation (72.22%)were high-er than that of VA tortuous (28.57%)and VA normal (12.50%)patients in cervical vertigo group.Conclusion The application of CDUS combined with CTA or CE-MRA could provide valuable diagnostic and therapeutic information for cervical vertigo in the as-pects of change in vascular morphology and cerebral hemodynamics,which could further provide objective basis for clinical diagnosis and treatment.

Objective To assess the drug sensitivity of pancreatic cancer cells based on real-time cell analysis and provide a reference for individualized diagnosis and treatment of pancreatic cancer.Methods Three human pancreatic cancer cells lines SW1900, Capan-2 and PANC-1 were selected and treated with gemcitabine hydrochloride and tegafur gimeracil oteracil potassium capsules, respectively.After 24 hours of culture, the cells were treated with the two drugs in gradient concentration.The cell growth curves before and after the drug administration was monitored using a real-time cells analyzer and the growth inhibition rates (IC50) of the drugs of the pancreatic cancer cells were calculated.At the same time, the cells in the cell culture plate were treated with the drug, and acridine orange/ethidium bromide (AO/EB) staining and laser scanning confocal microscopy were performed to observe the changes of cells after the drug administration.Results 72 hours after the drug administration, IC50 values for the three cell lines were different.The IC50 values of gemcitabine hydrochloride for SW1900, Capan-2 and PANC-1 cells were 1.69 μmol/L, 10.05 μmol/L and 12.74 μmol/L, respectively.The IC50 values of tegafur capsule for SW1900, Capan-2 and PANC-1 cells were 180.29 μmol/L, 765.70 μmol/L and 95.57μmol/L, respectively.AO/EB staining confirmed the reliability of IC50.Conclusions SW1900 and Capan-2 cells can be used as the control for gemcitabine hydrochloride and tegafur gimeracil oteracil potassium capsules to establish cell models for drug screening in vitro, which provides a reference for the application of the technology in anticancer drugs screening.

The content related to the quality during life-cycle in operation of medical device includes daily use, repair volume, preventive maintenance, quality control and adverse event monitoring. In view of this, the article aims at discussion on the quality evaluation method of medical devices during their life cycle in operation based on the Analytic Hierarchy Process (AHP). The presented method is proved to be effective by evaluating patient monitors as example. The method presented in can promote and guide the device quality control work, and it can provide valuable inputs to decisions about purchase of new device.
Decision Support Techniques ; Durable Medical Equipment ; standards ; Equipment Design ; Quality Control