A representative nitrogen doped titanium dioxide sample (denoted as N-doped TiO_2) was prepared by treating nanotubular titanic acid (H_2Ti_2O_5·H_2O,NTA in short) in flowing NH_3 at an elevated temperature of 500℃ for 4 h.The sub-band structure within Eg of N-NTA-500 was determined by means of photoluminescence (PL) spectrometry and ultraviolet-visible light-near infrared (UV-Vis-NIR)diffusion reflectance spectrometry (DRS).Moreover,the photocatalytic activity of N-NTA-500 was evaluated by performing the redox reaction of propylene under the irradiation of alternate on-and-off visible light,using commercially available P-25 titania as a comparison.It was found that a large amount of single-electron-trapped oxygen vacancies (denoted as Vo~·) formed a sub-band within the Eg of N-NTA-500,contributing to extend the optical absorption of N-doped TiO_2 into visible light region.The sub-band width,the bottom energy above the top edge of valence band,and the top energy below the bottom edge of conduction band were determined to be 0.24 eV,1.79 eV,and 0.99 eV,respectively.Results and discussion:Each of the four PL spectra at an excitation wavelength of 360-390 nm can be deconvoluted into two bands.The peak positions of the two deconvoluted PL bands for each of the four PL spectra at λ_(ex)=360-390 nm are unchanged and located at 465 nm and 570 nm,respectively,implying that they are not originated from the back band-to-band transition but the e~--h~+recombination on two surface states.The band at 465 nm represents the emission on shallow surface state (2.66 eV above valence band edge),while that band at 570 nm represents the emission on deep surface state (2.17 eV above valence band edge).Moreover,the PL peak intensity decreases with increasing excitation energy,indicating that the photogenerated energy-enriched electrons are firstly relaxed to the bottom of the conduction band and then transferred to the surface state,and the larger the value of E_(ex),the more the energy loss.Therefore,it can be concluded that the excitation at λ_(ex)≤400 nm for N-NTA-500 is dominated by band-to-band transition,while E_g of the N-doped TiO_2 is identical to that of TiO_2 (anatase),I.e.,3.1 eV at 400 nm.The emission mechanism with respect to band-to-band transition of N-NTA-500 in an excitation wavelength range of 360-390 nm is depicted.

Objective To observe the clinical efficacy of warm needling in treating neck-back myofasical pain syndrome. Method Sixty patients with neck-back myofasical pain syndrome were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by warm needling therapy, while the control group was by electroacupuncture. The short-form McGill Pain Questionnaire (SF-MPQ) and Physion MD muscle resistance detector (Japan) were adopted to measure the count of tender points (red points and yellow points), Pain Rating Index (PRI), Visual Analogue Scale (VAS), and Present Pain Intensity (PPI) before and after the treatment, and the clinical efficacies were also compared.Result The total effective rate was 83.3% in the treatment group versus 66.7% in the control group, and the difference was statistically significant (P<0.05). The SF-MPQ scores (PRI, VAS, and PPI scores) were significantly changed in the treatment group after the intervention (P<0.05). In the control group, VAS and PPI scores were significantly changed after the treatment (P<0.05). After treatment, there were significant differences in comparing the SF-MPQ scores between the two groups (P<0.05). The counts of red and yellow tender points were significantly changed in the treatment group after the intervention (P<0.05). The count of red tender points was significantly changed in the control group after the intervention (P<0.05). After the treatment, there were significant differences in comparing the number of red and yellow tender points between the two groups (P<0.05).Conclusion Warm needling is an effective approach in treating neck-back myofascial pain syndrome.

Background and purpose:The study hasfound that tumornecrosis factor-related apoptosis inducing ligand (TRAIL) can enhance the cytotoxic effect of chemotherapeutic drugs on tumor cells. The aim of our study was to investigate the effects of the tumor TRAIL and cisplatin combined application on the growth and apoptosis of human ovarian cancer cell lines SKOV3 and OVCAR3, and the possible mechanism.Methods:Under the combined application of cisplatin and TRAIL, MTT method and flow cytometry were used to detect the proliferation and apoptosis of SKOV3 of OVCAR3 cells; the mRNA expression levels of death receptors, DR4 and DR5, were detected by real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR). At the same time, the protein expression levels of DR4 and DR5 were detected by Western blot.Results:SKOV3 and OVCAR3 cells were sensitive to TRAIL protein, and with the increasing of TRAIL protein concentration, cell growth inhibitory rate up to 64%. When the
combination application of TRAIL and cisplatin, the inhibition rate of the two cells reached more than 92%, and the two drugs showed high synergistic effect, compared with the single drug group (P<0.05). Flowcytometry analysis indicated that the synergistic killing effect of TRAIL and cisplatin was mainly due to the cell apoptosis. RTFQ-PCR and Western blot detection results showed that the DR4 and DR5 were up-regulated under the combined application of TRAIL and cisplatin.Conclusion:In vitro, TRAIL and cisplatin combined application can signiifcantly inhibit the proliferation of human ovarian cancer cells and induce tumor cell apoptosis. TRAIL can obviously enhance the sensitivity of cisplatin to tumor cells. The mechanism may be related to the increased death receptor DR4 and DR5 expression level.

OBJECTIVE To analysis the effect of air disinfection by using nanometer light catalysis decontamination machine in operating room. METHODS By compare the effectiveness of air disinfection both by using nanometer light catalysis decontamination machine and ultraviolet rays light. RESULTS The result of tests is 0 CFU/m~2 by nanometer light catalysis decontamination machine and 33.3 CFU/m~2 by ultraviolet rays light in unmanned environment;By different groups: F=220.423,P=0.000,P

AIM To determine the contents of chemical consituents in Lycii Fructus from Qaidam Basin.METHODS Spectrophotometry was adopted in the content determination of polysaccharides,total flavonoids and carotenoid.HPLC was applied to the content determination of betaine and scopoletin.Kjeldahl method was used for the content determination of protein.Then principal component analysis was performed.RESULTS The contents of carotenoid,betaine and scopoletin in samples from six growing areas showed obvious differences (P ＜ 0.05),while those of polysaccharides,total flavonoids and protein exhibited no obvious differences (P ＞ 0.05).The contents of various constituents in samples at three picking time also had no obvious differences (P ＞ 0.05).The comprehensive score of principal components of samples from Delingha City was the highest,followed by that from Ulan County.CONCLUSION The quality of Lycii Fructus from Qaidam Basin from Delingha City is the best.

The author generally analyzed "Eight-Profiles" theory of traditional Chinese medicine before the Qing Dynasty, specifically, elucidated the meaning, theory origin, evolution and controversy of "Eight-Profiles" theory.

The article made a study on the earliest literature and the initial time of the "Five Orbiculi" theory in TCM. By analyzing the masons for different locations of "Five Orbiculi" recorded in ancient TCM books, the author argued against that the term of "Five Orbiculi" originated from ancient India and the "Five Orbiculi" theory was a product with the combination of traditioinal Chinese and Indian cultures. The author further put forward that the "Five Orbiculi" theory most probably was a Chinese traditional medical innovation under the influence of Internal Classic of Medicine, a great development to the theory recorded in Miraculous Pivot On Serious Confusion.

Objective To examine the consistency between asthma children's self-assessment health-related quality of life and their proxies' assessment, to give theoretic basis of clinical treatment for asthma children. Methods Parents and asthma children completed the PedsQLTM 4.0 Generic Core Scales and PedsQLTM 3.0 Asthma Module during an outpatient visit or in the hospital. Wilcoxon signed rank test and ICC were used to compare the difference between asthma children's self-report and their proxies' report. Results The findings indicated that neither the total scores nor domain scores (except physiological domain) between asthma children's self-reports and proxies' reports (parents most of all)showed inconsistency. The value of ICC was not less than 0.7 except physiological dimension and showed consistency. Some different information in several domains was founded by the scores after layered by age. Conclusions There are consistency between children's self assessment of health-related quality of life and their proxies' assessment based upon results of PedsQLTM scale.

Objective To evaluate the efficiency and toxicities of Gemcitabine combined with S -1 cap-sule in the treatment of advanced esophageal cancer .Methods Twenty-nine patients with advanced esophageal cancer were treated with S-1 capsule 80 mg/m2 ,twice daily for 14 days and Gemcitabine (1 000 mg/m2 ) was in-travenously administrated on day 1 and 8.The chemotherapy was repeated every 21 days.After two consecutive treatment circles ,the short term efficacy ,adverse effects and follow -up condition were evaluated .Results One case showed complete remission (CR),11cases showed partial remission (PR),9 cases showed stable disease (SD),8 cases showed progressive disease(PD).The responsive rate(CR+PR)was 41.4%;the illness control rate(CR+PR+SD)was 72.4%.The major adverse events were myelosuppression ranging from grade Ⅰto Ⅲ, hand-foot syndrome,disgusting and the damage of liver function .Some patients displayed the damage of liver function.The median time to progress ( mTTP) was 6.9 months.The median 1-year survival rate was 64.3%. Conclusion The combination of Gemcitabine and S -1 capsule is an effective and well -tolerated method for the patients with advanced esophageal cancer .

Objective To study the effects of acetylcholine combined with vincristine (VCR) on A549 cells, furthermore to study the influence that is due to affecting the expression of the two proteins bcl-2 and p53. Methods MTT assay was used to analyze growth inhibition effect of acetylcholine alone and combined with IC50VCR on A549 cells.The expression of bcl-2 and p53 was measured by immunohistological chemistry technique(IHC) and Western blot.Results The inhibition rates for A549 cells with 0.1,1 and 10 μmol/L acetylcholine were (0.050±0.032)％,(0.101±0.021)％,(0.169±0.015)％.The inhibition rates for tumor cells with 0.1,1 and 10 μmol/L acetylcholine joint 0.2 μg/ml VCR were (0.529±0.023)％,(0.545±0.011)％,(0.589±0.015)％,the difference was statistically significant compared with the VCR group (q'values were 1.09,1.37,1.83,P＜0.05).Acetylcholine alone exerted inhibitory effect on A549 cells in a concentration dependent manner and significanrtly enhanced its sensitivity to VCR (P＜0.05).acetylcholibne (0.1-10 μmol/L)combined with IC50VCR decrased the expression of bcl-2 and p53 (P＜0.05).Conclusion Acetylcholine alone and combined with VCR can inhibit the growth of A549 cells. Significant synergistic effect between acetylcholine and VCR is found in inducing cell apoptosis by changing the expression of bcl-2 and p53.