Objective To explore the effect of the whole course nursing intervention on the clinical efficacy and postoperative complications in patients with colonic surgery .Methods Seventy patients with colonic surgery were chosen and divided into the control group and the observation group according to the random number table, each with 35 cases.The control group received the routine nursing after the operation , and the observation group received the whole course nursing intervention .The clinical efficacy and the incidence rate of postoperative complications were observed in two groups .Results The total effective rate of treatment was 88.57%in the observation group, and was better than 65.71% in the control group, and the difference was statistically significant (χ2 =5.185 2, P <0.05 ).The incidence rate of postoperative complications was 17.14%in the observation group, and was lower than 31.43% in the control group, and the difference was statistically significant (χ2 =4.480 0,P <0.05).Conclusions The whole course nursing intervention in patients with colonic surgery can effectively improve the clinical efficacy , and reduce the incidence of postoperative complications , and improve the prognosis , and accelerate the speed of rehabilitation , and improve the patients ’ quality of life .

Objective:To analyze the characteristics of viral isolation and unique recombinant from untreated HIV-1 patients infected through sexual transmission and injection drug use, so as to provide evidence for understanding the biological characteristics and precise prevention and control of HIV-1 infection in different transmission routes.Methods:In view of the different HIV-1 transmission risks, newly diagnosed untreated HIV-1 patients from Beijing, Guangxi and Sichuan were carefully selected. Venous blood was collected to detect the viral load and CD4 + T cell count, and peripheral blood lymphocytes were isolated for virus isolation. Viral RNAs were extracted from the virus supernatant, and the near full-length genome sequences were obtained using in-house method, then the recombination patterns were determined. Results:Among the 65 HIV-1 infection, 32(49.2%), 20(30.8%) and 13(20.0%) were infected via men who have sex with men (MSM), heterosexual and injection drug use (IDU), respectively; genotypes mainly included 26(40.0%) CRF07_ BC, 23(35.4%) CRF01_ AE, and 9(13.8%) unique recombinant types (URFs). A total of 46 HIV-1 clinical strains were isolated. The positive rate of HIV-1 isolation was significantly negatively correlated with CD4 + T cells ( X2=4.22, P=0.04), but positively correlated with viral load ( X2=22.4, P<0.001); the multi-variate generalized estimating equations(GEE) model analysis of HIV-1 P24 antigen content showed similar result. In addition, GEE model showed a positive correlation between viral P24 antigen content and virus-producing culture time (52.14, 95% CI: 9.42~94.87, P=0.017). Viral growth curve analysis showed that the level of viral P24 antigen in MSM Group was significantly higher than that in heterosexual group and IDU group (adjusted P values were p<0.01 and P<0.05, respectively), on the 14th day after culture. The proportion of URFs in MSM Group was higher than that in heterosexual group, and the recombinant breakpoints in MSM Group were more than that in heterosexual group. Conclusions:MSM population was more sensitive to HIV-1 virus isolation; there was unique diversity of recombinant forms of HIV-1 among those with sexually transmitted infections, especially in the MSM population.