Objective To explore preventive effect of low-dose sufentanil combined with parecoxib sodium for hyperalgesia of remifentanil anesthesia after postoperative.Methods A total of 120 patients undergoing elective abdominal surgery under general anesthesia was randomly divided into three groups that were given intraoperative remifentanil infusion.At the 30 minutes before surgery,group A were given intravenous sufentanil 0.1 μg/kg combined with 40 mg parecoxib sodium,group B were given intravenous sufentanil 0.2 μg/kg combined with 40 mg parecoxib sodium,and group C were given normal saline 5 ml.Postoperative recovery time,extubation time,postoperative 1,2,6,12,and 24 h visual analog scale (Visual Analogue Scale/Score,VAS) score,and after 24 h additional analgesia number of cases and the restless occurrence of three groups were compared.Results Awakening time and extubation time of group B was significantly longer than groups A and C [(10.9 ± 1.3) min vs (8.6 ± 0.6) min,(8.2 ± 0.7) min;(14.0 ± 2.1) min vs (10.7 ± 2.2) min,(10.8 ± 1.2) min,all P ＜ 0.01].Each point VAS scores of groups A and B were significantly lower than group C (P ＜0.01).After 24 h,the number of cases additional analgesic medication,and restlessness occurrence of group C were significantly more than the number of cases of groups A and B (P ＜ 0.01),the number of cases and the restless occurrence after 24 h additional analgesic drugs of group A were more than group B,but there was no significant difference between two groups (P ＞ 0.05).Conclusions Low dose sufentanil together with parecoxib sodium can inhibit after-remifentanil-induced hyperalgesia,and will not appear awakening time and extubation time delays and safe.

Objective To explore the clinical effects of flurbiprofen axetl injection on the treatment of postoperative pain in patients with laparoscopic appendectomy and on the platelet aggregation function.Methods From April 2013 to October 2015,100 laparoscopic appendectomy patients were randomly divided into observation and control groups,50 cases in each group.Observation group was injected flurbiprofen axetl injection 1 mg/kg,the control group was injected normal saline 5 ml.Analgesic effect,platelet aggregation function,and side effect were observed for two groups.Results Activities and resting visual analogue scale (VAS) score were significantly lower after flurbiprofen axetl injection in observation group (P ＜ 0.01),while activities and resting VAS score were not significantly decreased after normal saline used (P ＞ 0.05).Compared to those in control group,activities and resting VAS score at 30 min,1 h,2 h,4 h,and 6 h after flurbiprofen axetl injection in observation group were significantly lower (P ＜0.01).Platelet aggregation level before and after medication in two groups were no significant change (P ＞ 0.05).Drug side effects in two groups were no difference (P ＞ 0.05).Conclusions Patients with laparoscopic appendectomy do well with flurbiprofen axetl injection,and no obvious side effects.It is worthy of clinical application.

Aim To explore the anti-proliferation effects of curcumin trinicotinate ( CurTn ) on vascular smooth muscle cell ( VSMC ) and its mechanism. Methods The cells were cultured in DMEM supple-mented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinD1 and p-ERK1/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What’ s more, CurTn could increase the G1 phase pro-portion of cell, decrease the S phase proportion and the expression level of PCNA protein. It was also found that CurTn significantly inhibit the protein expression of p-ERK1/2 and Cyclin D1 . Conclusion CurTn may inhibit the proliferation of VSMC via downregulating the expression of CyclinD1 and p-ERK1/2 .

The protein kinase B(Akt)pathway can regulate the growth,proliferation,and metabolism of tumor cells and stem cells through the activation of multiple downstream target genes,thus affecting the development and treatment of a range of diseases.Thioesterase superfamily member 4(THEM4),a member of the thioesterase superfamily,is one of the Akt kinase-binding proteins.Some studies on the mechanism of cancers and other diseases have shown that THEM4 binds to Akt to regulate its phosphorylation.Initially,THEM4 was considered an endogenous inhibitor of Akt,which can inhibit the phosphorylation of Akt in diseases such as lung cancer,pancreatic cancer,and liver cancer,but subsequently,THEM4 was shown to promote the proliferation of tumor cells by positively regulating Akt activity in breast cancer and nasopharyngeal carcinoma,which contradicts previous findings.Considering these two distinct views,this review summarizes the important roles of THEM4 in the Akt pathway,focusing on THEM4 as an Akt-binding protein and its regulatory relationship with Akt phosphorylation in various diseases,especially cancer.This work provides a better understanding of the roles of THEM4 combined with Akt in the treatment of diseases.

Nucleic acid aptamer is an oligonucleotide sequence screened by the exponential enrichment ligand system evolution technology (SELEX). Previous studies have shown that nucleic acid aptamer has a good application prospect in tumor diagnosis and treatment. Therefore, we reviewed the selection and identification of nucleic acid aptamer of lung cancer cells in recent years, and discussed the effect of aptamer as targeting drugs and targeting vectors on the diagnosis of tumors, which provide a new idea for early diagnosis and treatment of tumor.