1.Building the Reference Range of Low Hemoglobin Density(LHD%) of Healthy Population in Humen District of Guangdong and Discuss Its Clinical Application Value
Jinquan HE ; Caiming WU ; Guoqiang LI
Journal of Modern Laboratory Medicine 2017;32(2):86-88,91
Objective To establishe the reference range of low hemoglobin density (LHD %) of health population in Humen District of Guangdong,and discuss the clinical application value.Methods Randomly selected from January 2015 to September 2016 in Humen Hospital of Dongguan City physical examination of 1 650 cases of healthy people as control group,and selected the same period by the clinical diagnosis of iron deficiency anemia (iron-deficiency anemia,IDA) group,67 cases of patients with IDA Sysmex XT-1800i fully automatic blood analyzer were used respectively to detect blood routine,and then based on the average haemoglobin concentration (mean corpuscular hemoglobin concentration,MCHC),calculate the LHD%,and the calculation results were analyzed.Results The control healthy crowd LHD% value was 2.41% ± 0.85 %,95% reference range was 0.74 % ~4.08%,male was higher than the female,the difference was statistically significant (t =3.209,P<0.05).IDA patients before treatment LHD% was 30.97% ± 18.65%,significantly higher than the control group,the difference was statistically significant (t=19.536,P< 0.01),and with the level of hemoglobin (Hb) showed a negative correlation (r=-0.4313).LHD% values after treatment in patients with IDA group were significantly lower,but the recovery speed was slower than the Hb and MCHC.By ROC curve analysis,when the cut-off of LHD% value was 4.10%,the LHD% for IDA early diagnostic sensitivity of 96.78%,specificity of 100%.Conclusion Established the reference range of low hemoglobin density (LHD %) of healthy population in Humen district of Guangdong,and LHD% is IDA good indicator of early diagnosis and curative effect of dynamic monitoring.
2.Early diagnosis of lower extremity deep venous thrombosis and therapeutic effect of anticoagulation therapy with Roberts' age adjusted warfarin loading protocol in patients with acute ischemic stroke
Weiliang LUO ; Wu LIU ; Jinhua QIU ; Nanyan XU ; Caiming LI ; Hong WEN
Chinese Journal of Geriatrics 2009;28(2):104-108
Objective To explore the early diagnosis of lower extremity deep venous thrombosis (LDVT)and evaluate the therapeutic effect of anticoagulant therapy in hospitalized patients with acute ischemic stroke. Methods According to Wells model for suspecting lower extremity deep venous thrombosis,patients with suspected LDVT were confirmed by compression ultrasonography. If the patients diagnosed with LDVT had no contraindications to anticoagulant therapy,they were treated with low molecular weight heparin(LMWH)subcutaneous injection and oral warfarin at the same time.The dosage of oral warfarin was determined by Roberts'age adjusted warfarin loading protocol.LMWH was stopped when the patients'international normalized ratio(INR)was 2.0~3.0 for two consecutive days. Results From January 2003 to August 2007,2067 cases with acute ischemic stroke were admitted to the department of neurology in Huizhou Municipal Central Hospital including 18 cases with LDVT and the incidence was 0.9%.The patients with LDVT all had paralytic extremities including 13 left legs and 5 right legs with deep vein thrombosis.All the 18 cases were treated by anticoagulant including 17 cases with oral warfarin treatment for 3 months.Symptoms in all LDVT patients were eliminated.12 cases had been observed for one year and 5 cases for three months after they stopped taking warfatin.There were no patients with pulmonary thromboembolism and LDVT recurrence. Conclusions By using Wells model for suspecting LDVT,patients with acute ischemic stroke-complicated LDVT can be timely diagnosed.The goal of prompt and enough anticoagulant can be achieved according to Roberts'age adjusted warfarin loading protocol.Because of racial difference,population difference and other unknown factors,the incidence of acute ischemic stroke patients with complicated LDVT is much lower in Huizhou.It suggests that it should be unnecessary to use LMWH in patients with acute ischemic stroke to prevent LDVT in Huizhou.
3. Metabolomics study on biomarkers of hand, foot and mouth disease
Jinzhun WU ; Caiming WU ; Bizhen ZHU ; Guobing CHEN ; Guoping LU ; Xiaoshan DAI
Chinese Pediatric Emergency Medicine 2019;26(12):895-900
Objective:
To investigate potential biomarkers for different types of hand, foot and mouth disease(HFMD).
Methods:
Nuclear magnetic resonance(NMR)-based metabolomics methods were used to analyze the metabolic differences in serum of HFMD and healthy controls.Partial least squares discriminant analysis(PLS-DA)and orthogonal partial least square discriminant analysis(OPLS-DA)were used for analysis of metabolite profiles.Differential statistical analysis was performed by