Objective To evaluate application of fist clenching method with shaking hands in venipuncture to elderly angular patients. Methods 360 elderly angular patients were randomly divided into group A and group B with 180 cases in each group. Group A used fist clenching method with shaking hands, group B adopted traditional fist clenching method in venipuncture. Pain degree and one-time success rate of two groups were compared. Results One-time success rate of group A in venipuncture was obviously higher than group B, while pain degree of group A was less than group B. Conclusions Fist clenching method with shaking hands to elderly angular patients can raise success rate in venipuncture, and reduce pain degree of patients.

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Objective Analyzing the construction process and application of health appropriate technology database in Zhejiang province and proposing related considerations and recommendations for the database construction.Methods To design and construct the technology database system,and using excel software to calculate statistic data.Results Established a health appropriate technology database,which contains 826 technologies that can be selected and promoted by the demonstration bases in the whole province.Conclusions The establishment of the technology database increased the efficiency of health appropriate technology transformation at the provincial level,and also provided a reference for the national-wide generalizing of health appropriate technology.

Objective:To investigate the clinical phenotype and gene mutation in a child with developmental disorders caused by CTNNB1 gene mutation. Methods:Clinical data of a child with CTNNB1 gene mutation who was admitted to Xiamen Hospital of Fudan University Affiliated Pediatric Hospital in May 2017 were collected, whole exome sequencing technology was applied to verify the family lineage of the child, and the pathogenicity of mutation site was analyzed. Results:The patient was a 6 years and 1 month old male, with a clinical phenotype including mental retardation, motor developmental disorders, speech disorders, visual disorders (internal strabismus), microcephaly, and behavioral problems (social withdrawal, overdependence, etc.), as well as panic syndrome (i.e., sudden shrieking in response to auditory and visual stimuli, extensional rigidity of the body, etc., followed by short periods of general extensional rigidity). The whole exome sequencing results showed the presence of a de novo mutation c.283(exon4)C>T in the CTNNB1 gene, and the c.283(exon4)C>T mutation was interpreted as pathogenic (PVS1+PS2+PS1+PM2+PM) according to the American College of Medical Genetics and Genomics variant classification criteria and guidelines. No relevant genetic variants were found in the parental family verification. Conclusion:CTNNB1 gene mutation c.283(exon4)C>T can cause neurodevelopmental disorders, including mental retardation, motor developmental disorders, speech disorders, visual disorders, microcephaly and behavioral abnormalities.

OBJECTIVETo evaluate the positions and forms of temporomandibular joints (TMJ) with different vertical skeletal patterns in Class II division 1 patients.

METHODSCone- beam CT scans of 117 Class II division 1 adolescent patients (including 30 patients with high angle, 58 with average angle and 29 with low angle) were selected and reconstructed using Mimics 10.0 software. Fourteen measurements related to the positions and forms of condyle, forms of fossa and joint spaces were performed.

RESULTSCompared with high and average angle patients, the condyles in low angle patients located more posteriorly and inferiorly [the vertical distances between condyle and S was (20.67 ± 1.99)mm and the antero-posterior distances between condyle and S was (9.15 ± 1.61)mm]. The joint fossae were deeper [the fosse depths was (7.83 ± 1.06)mm]. The angles of posterior joint node bevels were bigger [the inclinations were (28.91 ± 3.94)° ]. The condyles were shorter, thicker, tipped more anteriorly and positioned more posteriorly [the proportions of post-positioned condyles of low angle, average angle and high angle patients were 26%, 4%, 0% respectively].

CONCLUSIONSClass II division 1 adolescents with different vertical skeletal patterns had different forms of fossae, and different positions and forms of condyles.

Adolescent ; Cone-Beam Computed Tomography ; Humans ; Malocclusion ; Malocclusion, Angle Class II ; Mandibular Condyle ; anatomy & histology ; Temporal Bone ; Temporomandibular Joint ; anatomy & histology ; diagnostic imaging