Objective:This study aimed to investigate the relationship between estrogen receptor (ER) and Bcl-2 in cervical can-cer and precancerous lesions. Methods:ER and Bcl-2 were evaluated by immunohistochemistry in 20 non-tumor tissues, 30 low-grade cervical intraepithelial neoplasias, 70 high-grade cervical intraepithelial neoplasias, and 80 cervical cancers. Results:The positive rate of ER was 75%in non-tumor tissues;this rate decreased with disease severity (χ2=24.266, P<0.01). The overexpression of ER protein was remarkably lower in cervical intraepithelial neoplasias (CIN) Ⅱ-Ⅲ and carcinomas than in non-tumorous cervices. Differences were not significant between CINⅠand non-tumorous cervices (χ2=0.751, P=0.386). Furthermore, ER expression was not correlated with histological type and tumor grade. The positive rate of Bcl-2 was 75.38%in squamous cell carcinoma. This rate increased with dis-ease severity (χ2=27.715, P<0.01). The Bcl-2 protein overexpression was remarkably higher in CINⅠ, CINⅡ-Ⅲ, and carcinomas than in non-tumorous cervices. Bcl-2 expression was also not correlated with histological type (P=0.500);by comparison, Bcl-2 expression was correlated with tumor pathological grade (χ2=4.862, P=0.027). The co-expression of ER and Bcl-2 was 38.57%and 15.38%in CINⅡ-Ⅲand squamous cell carcinoma, respectively;the differences between these two values were significant (χ2=9.108, P=0.003). A cor-relation between ER and Bcl-2 was observed in CINⅡ-Ⅲ(r=0.506, P<0.01);no correlation was observed between other groups. Con-clusion: ER expression was inversely correlated with Bcl-2 in cervical cancer progression. The co-expression of these markers may have an important function in the course of high-grade cervical intraepithelial neoplasia.

To establish a highly sensitive and specific method to detect the presence of Cryptosporidium homini, the RT-PCR-ELISA assay was tried, in which the primer with a biotin-labeled probe was designed to amplify fragment containing the highly variable region by multiple alignment between p23 gene of C.hominis and other Cryptosporidium spp. The RT-PCR was used to amplify the target fragment, and the amplified product was used to hybridize with the probe primer. The hybridized product was then captured on micro-plate wells coated with streptavidin and reacted with anti-digoxin antibody labeled with horse-radish peroxidase. This method of testing was then used for the detection of C.hominis in 22 clinical specimens and compared with the conventional methods of testing. It was demonstrated that the RT-PCR--ELISA for the detection of C.hominis was proved to be quite sensitive and specific. Its sensitivity was 100 times higher than that of the general PCR. From the result of clinic detection, the detection rate of RT-PCR-ELISA assay attained to 86%(19/22), while those of RT-PCR, sucrose floating method and anti-acid staining were 27%, 27% and 50% respectively. This result indicates that the RT-PCR-ELISA assay is more sensitive to detect C.hominis than the other three methods of testing.

Objective To explore the operation of grasping forceps and nursing staff made telescopic injection device were used for the clinical effect of Hemostasises thoracoscopic lobectomy. Methods 60 cases of thoracoscopic lobectomy for patients according to the date of surgery were divided into operation group and telescopic grasping forceps injection device group 30 patients were given hemostasia operation grasping forceps extraction hemostatic powder and hemostatic powder into the injection device for telescopic wound hemostasis treatment and preventive treatment, were compared between the two groups in the instrument body, completely hemostatic powder cover the wound time, evacuation time of endoscopic instruments, single hemostatic powder wound coverage, wound bleeding rate, the incidence of bleeding again installed. Results The telescopic injection device group and operation time grasping forceps group into cavity instruments, endoscopic evacuation equipment time showed no significant difference (P>0.05). Hemostatic powder completely cover the wounds of time (24.90 ± 5.90) s and (55.60 ± 25.60) s, there was significant difference between two groups (t=6.401, P<0.01). The single ended wound coverage of blood meal was 86.67%(26/30) and 76.67%(23/30), there was significant difference between two groups (χ2=2.016, P<0.05). Wound bleeding rate was 10.00%(3/30) and 33.33%(10/30), there was statistically significant difference between the two groups (χ2=4.812, P<0.05). The incidence of bleeding again to install powder was 10.0%(3/30) and 93.3%(28/30), there was significant difference between two groups (χ2=41.713, P <0.01). Conclusions The nursing staff made telescopic injection device assisted surgery were thoracoscopic lobectomy of the wound to stop bleeding and prevent rebleeding, wound coverage than grasping forceps, completely cover the wound operation less time than grasper, re install a low incidence of hemostatic powder, the whole process to shorten the operation time.

OBJECTIVE To explore the clinical practice differences based on GBZ 49-2007 and GBZ 49-2014 diagnostic criteria for occupational noise induced hearing loss.METHODS Two hundred and sixty-five noise workers who applied for occupational noise induced hearing loss diagnosis in Hangzhou Occupational Disease Prevention and Treatment Center from January 2016 to November 2023 were selected and diagnosed according to the diagnostic criteria of GBZ 49-2007 and GBZ 49-2014 for noise induced hearing loss.RESULTS The diagnostic rate of occupational noise induced hearing loss in GBZ 49-2014 standard was 53.58%,which was significantly higher than the 42.64%in GBZ 49-2007 standard(P<0.05).The κ value of the diagnostic consistency between the GBZ 49-2014 standard and the GBZ 49-2007 standard for occupational noise induced hearing loss was 0.761,P<0.05,indicating good diagnostic consistency with a diagnostic consistency rate was 86.04%.The diagnostic rate of occupational noise induced hearing loss(GBZ 49-2007)among workers with work experience≥10 years was 59.05%,which was higher than that of workers with work experience<10 years(P<0.05).The diagnostic rate of occupational noise induced deafness in workers with work experience≥10 years(GBZ 49-2014)was 72.38%,which is higher than that of workers with work experience<10 years(P<0.05).According to the diagnosis of occupational noise induced hearing loss in GBZ 49-2007 and GBZ 49-2014,patients with work experience≥10 years had a higher severity of occupational noise induced hearing loss than those with work experience<10 years(P<0.05).CONCLUSION Compared to the GBZ 49-2007 standard,the GBZ 49-2014 standard will increase the diagnostic cases of occupational noise induced hearing loss;Under the GBZ 49-2007 and GBZ 49-2014 standards,the diagnosis and severity of occupational noise induced hearing loss are related to length of service.

Objective To explore the application of failure mode and effect analysis in management of precision instrument in operating room of ophthalmology department.Methods We designed the handover process between operating room of ophthalmology department and supply room,the process of using precision instrument,the workflow of instrument management in nurses. And then,we assessed and analyzed the potential failure mode in the management of precision instrument with the method of failure mode and effect analysis.Besides,failure mode was also evaluated and improved.Results There were failure modes with high risk in the management of precision instrument including that the handover process between operating room of ophthalmology department and supply room was not precise enough (risk priority number,RPN=448);the staff of handover process was not fixed (RPN=360);there was no full-time cleaning staff in supply room (RPN=288);use registers in nurses and the check in precision instrument were all in a lack of standardization (RPN=210, 180) and so on. After adopting targeted corrective actions,the corresponding scores of RPN fell to 0,0,40,70, 90.Conclusions The application of failure mode and effect analysis in management of precision instrument in operating room of ophthalmology department has good effects with no related adverse event on precision instrument.

Objective To explore the effects of steering group model on knowledge of pelvic floor muscle exercise and postoperative discomfort and complications after cesarean section, so as to improve the effect of pelvic floor muscle exercise. Methods A total of 108 patients who underwent cesarean section from August 2015 to August 2016 were randomly divided into experimental group and control group by the random number table method, with 54 cases in each group. The puerperae in the control group were treated with conventional pelvic floor muscle exercise. In the experimental group, the nursing staff and the patients' one-on-one steering group was set up and the individualized pelvic floor muscle exercise was carried out during the hospitalization. The puerperae of the two groups were compared in stress urinary incontinence, pelvic organ prolapse, defecating unfinished feeling, pelvic bulge, lower genital tract discomfort, the level of cognition and behavior of pelvic floor muscle exercise and the puerpera satisfaction in the reexamination after 6 weeks of discharge. Results The number of puerperae with stress urinary incontinence and pelvic organ prolapse in the experimental group was less than that in the control group, and the difference was statistically significant (P< 0.05). The number of puerperae with defecating unfinished feeling, pelvic bulge and lower genital tract discomfort in the experimental group was significantly less than that in the control group (P< 0.05). The score of knowledge level of the pelvic floor muscle in the experimental group was (4.60±1.38), and the exercise behavior score was (4.36±1.17). The score of knowledge level of the pelvic floor muscle in the control group was (2.26±1.27), and the exercise behavior score was (2.57±1.24). The differences were statistically significant (P<0.05). The satisfaction rate was 81.48% in the experimental group and 42.59% in the control group (χ2=5.793,P<0.05). Conclusions The establishment of the steering group for maternal recovery exercise guidance can improve the maternal knowledge of exercise, so as to reduce all kinds of discomfort and complications, improve pelvic floor muscle exercise effect and improve maternal satisfaction.

Objective:To explore the application effect of nursing based on transtheoretical model in patients with oral surgery.Methods:A total of 120 patients with oral surgery who were admitted to Department of Stomatology of the First Affiliated Hospital of Xinxiang Medical University from October 2019 to January 2020 were selected as research objects by the convenient sampling method. According to random number table, they were divided into the control group and the observation group, with 60 cases in each group. Patients in the control group were given routine nursing while patients in the observation group were given transtheoretical model-based nursing at basis of the control group. The amount of dental plaque, comfort of oral cavity, score of psychological state and degree of oral cleanliness before and after nursing were compared between the two groups.Results:After intervention, the amount of dental plaque and the score of oral comfort in the observation group were lower than those in the control group, and the differences were statistically significant ( P<0.05) . After intervention, SAS and SDS scores in the observation group were lower than those in the control group, and the differences were statistically significant ( P<0.05) . The degree of oral cleanliness of the observation group was better than that of the control group after intervention, and the difference was statistically significant ( P<0.05) . Conclusions:Nursing based on transtheoretical model can effectively reduce postoperative amount of oral plaque of patients with oral surgery, improve comfort of oral cavity and oral cleanliness and correct bad mood of patients, which is worthy of clinical application.