Objective To investigate the immune effect of neonatal hepatitis B vaccination and its once more immune effect in low or no responders .Methods Screening Songzi city MCH 2 000 cases of hepatitis B vaccine 7-12 months baby's first immunization effect ,analyze the impact of the prime factors for early response in children with low or no free choice 10μg Hansenula yeast recombinant hepatitis B vaccine or 5μg hepatitis B vaccine ( HepB-SC) , carried revaccination(Immune again),then analyzed the effect of free 1,3 times.Results (1) Primary immune response rate of neonatal hepatitis B vaccine in 2 000 cases was 92.2%,the average anti-HBsGMC was (321.88 ± 100.45) IU/L,in which the HBsAg positive persons and birth in the township hospital neonatal response rate was low that had a statistically significant (χ2 =30.61,47.58,all P <0.05) difference with the children whose parents'HBsAg was negative and borned in hospitals above the county level;( 2 ) GMC content was increase in once more immune at 1,3 times than before,the difference was statistically significant (t=98.32,102.15,all P<0.05),and the 3 time is higher than the 1 time once more immuneof levels of GMC ,the difference was statistically significant ( t=98.55,102.33,all P<0.05),content of GMC is higher in once more immune of Hansenula hepatitis B vaccine at 1, 3 times when compared with the recombinant hepatitis B vaccine , the difference was statistically significant ( t =104.11,124.31,all P<0.05).Conclusion Low and non-responders'proportion is high in the children whose par-ents'HBsAg is positive and be born in the primary hospital ,which has a higher response rate after once more immune , Hansenula hepatitis B vaccine has a better immune effect .

Objective To detect different animal erythrocyte hemolysins titer,and compare the application of these hemolysins in immunological experimental teaching,for selecting the better method of preparing high titer hemolysin for experimental teaching of medical immunology.Methods A total of 40 experiment rabbits were divided into 4 groups in this study,and immunized by sheep red blood cell(SRBC) and porcine red blood cell(PRBC) through different immunization procedures to prepare the hemolysin,detect and compare these 4 groups hemolysins titer by the complement hemolysis test.Results Rabbit Anti-SRBC in the group A was 1∶4 800,rabbit Anti-PRBC in the group B was 1∶1 200,rabbit Anti-SRBC in the group C was 1∶1 000,rabbit Anti-PRBC in the group D was 1∶200.Conclusion The hemolysin titer of the rabbit Anti-PRBC was lower than that of the rabbit Anti-SRBC by the same immunization procedures,and the immunization procedure by intradermal multi-point and auricular vein injection is the better method of preparing high titer hemolysin,so PRBC could replace SRBC as antigen,and immunize the rabbits for preparing hemolysin,which could be used in experimental teaching of medical immunology.

Objective To use tri-operator breast blood oxygen imaging in diagnosis of early stage breast cancer.Methods To analysis and diagnosis eighty cases with the technology of breast blood oxygen imaging.Results The accurate rate of the diagnosis made by technology of breast blood oxygen imaging was 93.75%,the sensitivity of diagnosis was 90.63%,the specificity was 95.83%.Conclusions The technology of breast blood oxygen imaging without the radiation may be a better methods to diagnosis the breast diseases,which has the higher sensitivity than infrared rays examination on breast cancer diagnosis.