To simplify the preparation of neuraminidase in screening influenza neuraminidase inhibitors,the neuraminidase gene of H5N1 influenza A virus was optimized for high expression in mammalian cells and cloned into pcDNA4/TO vector. The recombinant plasmids were transfected into T-REx 293 cells to establish stable cell lines,in which the expression of neuraminidase was induced by tetracycline. Unlike from virions,the preparation of neuraminidase became conveniently and safely from these stable cell lines,which would facilitate developing high throughput assay to screen neuraminidase inhibitors. More than 3000 natural extracts and herbal components were screened in the study. Baicalin and baicalein were found to inhibit oseltamivir-sensitive and oseltamivir-resistant neuraminidase at similar level,furthermore,their anti-influenza activity was confirmed by plaque assay and virus inhibition assay.

The need for an efficacious HIV/AIDS vaccine remains the highest priority of the world HIV/AIDS agenda. The generation of an efficacious HIV/AIDS vaccine proves an enormous scientific challenge. This article reviews the neutralizing antibody problem, elusive immune protection, immunogen design, pre-existing anti-vector immunity and design of phase 3 vaccine trials and the challenges and opportunities in development of HIV/AIDS vaccine are discussed.

The outbreaks caused by coronavirus (CoV) infections including severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS) and COVID-19 have emerged in recent years, posing a continued threat to public health. Highly pathogenic CoV infections usually induce a series of immune responses, including innate immunity, humoral immunity, cellular immunity and mucosal immunity. These responses not only are critical to suppressing and eliminating the invasion of viruses, but also play an important role in immunopathological changes and disease progression. A deep understanding of the dual roles of immune responses will help to elucidate the pathogenic mechanism of CoV and maintain the balance between immune protection and immunopathology, which will be conducive to the research and development of safe and effective CoV vaccines. This review summarized the dual roles of immune responses in highly pathogenic CoV infections and discussed the implications for the research and development of novel CoV vaccines.

Currently, human immunodeficiency virus (HIV) surveillance mainly relies on sentinel surveillance and the HIV/acquired immunodeficiency syndrome (AIDS) case reporting system to calculate the HIV infection rate, the number of newly reported HIV cases, and the HIV-related mortality rate, while theses measures are not able to directly estimate the HIV incidence. National-level research is conducted to investigate the characteristics of drug-resistant strains of HIV. HIV infection has the characteristics of a covert progression and a long-term latent phase, making it difficult to identify individuals in the acute infection stage. Conventional monitoring method struggles to determine the infection time of individuals, thereby introducing potential biases in the estimation of the incidence and impacting the comprehensive exploration of disease risk factors and the assessment of intervention measures. Recently, test for recent infection (TRI), as one of AIDS epidemic surveillance and intervention assessment measures, has become a vital way to estimate HIV incidence by testing the cross-sectional specimens. TRI can identify recent HIV infection and long-term HIV infection, consisting of serological and molecular method. Serological assays have been widely used because of their low cost, high accuracy of HIV infection incidence estimate and long development history, and their accuracy and simplicity have achieved significant progress in recent years. According to introduct the principle, accuracy and application of TRI, this paper reviews the latest progress, advantages, and limitations of TRI.

Objective To know the head nurses′ mental health in Huaian tertiary hospitals and researching the effects of working experience, job title and departments on head nurses′mental health, so as to provide references for the interventions. Methods Totally 202 head nurses from 7 tertiary hospitals in Huaian area were selected as the research object. The general questionnaire and the symptom checklist-90 ( SCL-90) were used to conduct the investigation. A total of 202 questionnaires were sent out and 183 valid questionnaires of them were taken back, and the effective rate reached up to 90. 59%. Results Except for the symptoms of terror factor, hostile, the remaining seven symptoms factors, SCL-90 total score and the number of positive items were all significantly higher than the national norm (P<0.01). As the survey results showed, the difference in tenure matron SCL-90 total average score, the score of somatization, depression, coercion, anxiety and interpersonal relationship, paranoia and psychotic average were statistically significant ( P<0.05) . Differences in the scores of depression and paranoid symptoms with different professional titles were statistically significant differences ( P<0. 05 ) . There were statistically significant differences in the scores of somatic, depression, obsessive-compulsive and anxiety symptoms among head nurses in different departments (P<0.05). Statistical index positive rate:head nurses′total SCL-90 score was over 160 points, and accounted for 30.60%;individual positive items were >43, and accounted for 32. 79%; The positive rates of each factor were more than 10%except hostility and terror. Conclusions The mental health situation of head nurses in tertiary hospitals of huaian is not optimistic, and the authorities and the head nurses need to take active interventions to promote their mental health and improve the quality and efficiency of care work.

Objective:To investigate the clinical characteristics of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and corona virus disease 2019 (COVID-19) infection.Methods:Clinical data of AECOPD patients over 65 years old who were diagnosed in the Respiratory and Emergency Departments of the Dongzhimen Hospital, Beijing University of Chinese Medicine from September 2022 to September 2023 were collected. AECOPD patients were divided into a COVID-19 group ( n=29) and a non COVID-19 group ( n=31). The platelet count, white blood cell count, lymphocyte count, neutrophil count, neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), C-reactive protein (CRP), procalcitonin (PCT), partial pressure of oxygen (PO 2), partial pressure of carbon dioxide (PCO 2), D-dimer (D-D), and interleukin-6 (IL-6) were compared between two groups of patients upon admission Confusion, Uremia, Respiratory, BP, Age 65 Years (CURB-65) was used to compare length of hospital stay, AECOPD grading, and mortality endpoint days. Results:There was no statistically significant difference in platelet count, white blood cell count, lymphocyte count, neutrophil count, NLR, and PLR between the COVID-19 group and the non COVID-19 group (all P>0.05). The proportion of males, CRP, PCO 2, D-D, IL-6, and CURB-65 scores in the COVID-19 group were higher than those in the non COVID-19 group, while PCT and PO 2 were lower than those in the non COVID-19 group, with statistically significant difference (all P<0.05). The proportion of AECOPD grade Ⅲ in the COVID-19 group was significantly higher than that in the non COVID-19 group, and the progression rate of the disease was higher in the COVID-19 group (37.9% vs 22.6%, P<0.05). COVID-19 was an independent influencing factor for the progression of AECOPD. Conclusions:Patients over 65 years old with AECOPD infected with COVID-19 have a more pronounced inflammatory response, and CRP, IL-6, and CURB-65 scores can be used as indicators to evaluate the degree of inflammation. AECOPD infected with COVID-19 are more prone to coagulation disorders, hypoxemia, more severe illness, and easier progression, suggesting that COVID-19 infection is an independent influencing factor for the progression of AECOPD.

Graphene,as a new type of two-dimensional nanomaterial,has unique physical and chemical properties,which make it characterized by low resistivity,large specific surface area,good biocompatibility,and high chemical stability.It is expected to develop into an ideal material for highly sensitive electrochemical sensors.With the development of electrochemical sensor technology,graphene based electrochemical sensors with high sensitivity,specificity,speed,and convenience can be used to detect various infectious disease patho-gens.This article summarizes the application of electrochemical sensor detection technology based on graphene nanomaterials in the detection of respiratory pathogens,mosquito borne infectious disease pathogens,intestinal infectious disease pathogens,and other infectious disease pathogens,providing new ideas for achieving rapid detection technology and methods for early detection and diagnosis of infectious diseases.