Objective: To investigate whether Irgm 1 impact CD4+T cell subsets in the experimental autoimmune encephalomyelitis.Methods: The Irgm1 heterozygous mice were backcrossed with C 57BL/6 Wt.mice for 10 generations to produce C57BL/6 Irgm1+/-mouse.C57BL/6 Irgm1+/-mice were intercrossed to obtain three genotypes:Irgm1-/-, Irgm1+/-and Irgm1+/+.To establish model of EAE ,C57BL/6 Wt.mice and Irgm1 knock out mice were immunized with myelin oligodendrocyte glycolprotein 33-55 ( MOG33-55 ) and the clinical symptoms were observed.The proliferation of lymphocytes to MOG antigen was detected with Methyl Thiazolyl Tetrazolium ( MTT).The infiltration of inflammatory cells in the spinal cords was observed through HE staining.The CD4+T cell subsets from lymph nodes ,spleens and CNS were detected by flow cytometry.Results:EAE model was induced successfully.The proliferation of T cells in lymph nodes in Irgm 1-/-mice was lower than Wt.mice.Quantitative analysis of flow cytometry indicates that , compared with Wt.mice,the level of Th1 subset was higher,Th17 was lower relatively in lymph nodes and CNS of Irgm1 knock out mice.Conclusion:Irgm1 knockout mice can be partially protected EAE spinal cord function and clinical symptoms .Irgm1 may play a key role at early stage of EAE ,which may use as an important molecular target for treatment of EAE.

Objective To study the patients with ankylosing spondylitis (AS) complicated with Sjgren′s syndrome (SS).Method Five patients were diagnosed and clinical features were analyzed.Results The 5 patients (5 AS/SS) had a mean age 46 8 years (range 33～63) at onset of the disease, 1 male and 4 females.The main clinical features of patients with both AS and SS were axial inflammatory back pain,sacroiliitis,dry eyes and/or dry mouth.HLA B27 in 3 patients and RF in 2 patients was positive.Conclusion The coexistence of AS and SS can be seen in clinical works.

Objective To explore the efficacy and safety of intermittent low-dosage intravenous (Ⅳ) cyelophosphamide (CTX) in treating lupus nephritis (LN) related chronic renal insufficiency. Methods Twelve patients with chronic renal insufficiency with azotemia caused by lupus nephritis received intermittent low-dosage eyclophosphamide therapy (200-400 mg/2～4 weeks) for 2 month to 5 years. We monitored their clinical outcomes and laboratory parameters changes. Results SLEDAI significantly decreased in all cases. Renal function was improved in varying grees. Nine cases had their renal function tests returned to normal range and another 3 cases were still in azotemia. No severe adverse effects occurred. Conclusion The treatment is effective and safe. But it is necessary to carry out large samples and long-term follow-up clinical studies to evaluate its effects on renal function changes.

Patients diagnosed to have acute coronary syndromes( ACS) were included in the study. All ACS patients were divided into non-diabetes group(40 cases) and diabetes group(80 cases) . Blood coagulation function was de-termined for all patients. In the diabetes group eighty patients were randomly divided into ticagrelor group (48 ca-ses) and clopidogrel group (32 cases) . After 5 days′treatment, platelet function was detected. Our study aimed to examine the effects of ticagrelor and clopidogrel on the platelet function.

BACKGROUND:Studies have shown that Panax notoginseng saponins (PNS) has extensive pharmacological basis for the treatment of cerebral ischemic injury, and animal experiments for treatment of brain injury by bone marrow mesenchymal stem cel transplantation are ongoing. However, little is reported about the combined use of PNS and bone marrow mesenchymal stem cel transplantation.
OBJECTIVE:To investigate the effect of PNS combined with bone marrow mesenchymal stem cel transplantation on traumatic brain injury in rats.
METHODS:Traumatic brain injury models were made in 60 Sprague-Dawley rats by hydraulic shock method. Then, model rats were randomized into model group, cel transplantation group receiving bone marrow mesenchymal stem cel transplantation and combined treatment group undergoing PNS combined with bone marrow mesenchymal stem cel transplantation. Nerve function recovery of the rats and protein expression of nerve growth factor in the brain tissue were evaluated and detected by Bederson scoring and western blot methods, respectively. Morphological changes of the brain tissue and apoptosis in cortical neurons were observed and detected by hematoxylin-eosin staining and TUNEL, respectively.
RESULTS AND CONCLUSION:Highest Bederson score was found in the model group, fol owed by the cel transplantation group and combined treatment group (P<0.05), while the protein expression of nerve growth factor was ranked as fol ows:the combined treatment group>the cel transplantation group>the model group (P<0.05). Inflammatory infiltration and brain edema in the brain were relieved markedly in the combined treatment group compared with the other two groups, and the number of apoptotic neurons was significantly reduced as wel . Our findings suggest that PNS combined with bone marrow mesenchymal stem cel transplantation can promote neurological recovery from traumatic brain injury by increasing the expression of nerve growth factor and reducing neuronal apoptosis.

Objective To predict the genes that affect endometrial receptivity through the differential expression of microRNA (miRNA) in eutopic endometrial tissues during implantation window in patients with endometriosis infertility. Methods Among infertile patients that received treatments at the Center for Reproductive Medicine,Peking University Third Hospital between May and December 2013, patients with endometriosis infertility were selected as endometriosis group (among the selected 17 cases, there were 6 cases with follicular phase endometrium and 11 cases with implantation window phase endometrium), and patients with tubal factor infertility were selected as control group (among the selected 19 cases, there were 7 cases with follicular phase endometrium and 12 cases with implantation window phase endometrium). (1)Implantation window phase endometrium was selected from 3 cases in each group. Using miRNA and mRNA joint gene sequencing and database for forecast results, as well as using the negative regulatory relationship between miRNA and mRNA, the intersection of target gene that negatively correlates with miRNA expression were obtained. The co-expression network of miRNA-mRNA wae constructed. Combined with the genes associated with endometrial receptivity found through bioinformatics method, the miRNA with core regulatory functionwas found. (2) Expand sample size to 14 cases for endometriosis group and 16 cases for control group.Reverse transcription (RT)-PCR technique was utilized to detect the expression of miR-142-5p, miR-146a-5p and miR-543 in endometrial tissues, and verify miRNA microarray results. Results (1) miRNA and mRNA microarray screening results showed that, among the endometrial tissues of patients with endometriosis infertility and with implantation window phase, 6 differentially expressed miRNA were indentified, among which miR-142-5p, miR-146a-5p, miR-1281, miR-940, miR-4634 showed significantly enhanced expression and miR-543 showed significantly inhibited expression. Sixty-three differentially expressed mRNA were indentified, among which 58 mRNA such as CADM1, IL-10RA, ITGAL and LPAR5 had significantly enhanced expression. Five mRNA such as HLA-DRB1,3,4,5 and SOHLH2 showed significantly inhibited expression. Thirty-six taget genes were found in consideration of negatively correlated miRNA expression with the genes, miRNA-mRNA co-expression network were constructed. The miR-543 was found at the core of the network. Targetscan and other database predicted that miR-142-5p, miR-146a-5p and miR-543 could act on various types of endometrial receptivity molecular corresponding marker genes such as HOX10, ITGAV, ITGB3, OPN, LIF, ESR, PGR, CDH1 and MMP. (2) RT-PCR test showed that the average levels of expression of miR-142-5p and miR-146a-5p in implantation window phase endometrium in endometriosis group were 8.3 ± 10.6 and 1.9 ± 0.8 respectively;the average levels of that in control group were 1.1±0.6 and 0.9±0.4, respectively. The difference was statistically significant (P=0.027, P=0.015), and was consistent with results from miRNA microarray test. The expression of miR-543 in tissues of follicular phase endometrium in endometriosis group (2.3±1.0) was significantly higher than that in control group (1.0 ± 0.4), and the difference was statistically significant (P=0.008). However, when comparing the expression of miR-543 implantation window phase endometrium in endometriosis group (1.2±0.6) with that in control group (1.5 ± 1.0), the difference was not statistical significant (P=0.890). Conclusions There are multiple differential expressions of miRNA in the implantation window phase endometrium tissues of endometriosis infertility patients, among which miR-142-5p and miR-146a-5p show significantly enhanced expression and may affect embryo implantation by acting on a variety of endometrial receptivity marker molecules. The expression of miR-543 in implantation window phase endometrium of endometriosis infertility patients is lower than that in the follicular phase, forewarned changes in the pattern of cyclic variation of miR-543, and may be the reason for affecting endometrial receptivity.

Objective:It is important to make sure hyperbaric oxygen chamber safe and stable for its generalized and high risk.Methods: The gas path, control system and operation of hyperbaric oxygen chamber should be maintained timing based on quality management and clinical using requirement.Results: Medical engineering technicians and clinical use of personnel to cooperate with each other, from the regular inspection, personnel management and preventive maintenance to make sure the safety use of hyperbaric oxygen chamber.Conclusion: Making relative system and emergence plan, strengthening train to users and strict management can make its used safe.

Objective To evaluate the efficacy and safety of transvaginal hydrolaparoscopic ovarian drilling for treatment of the polycystic ovary syndrome(PCOS)in clomiphene citrate-resistant infertile women.Methods Between February 2008 and November 2008,11 clomiphene citrate-resistant anovulatory women with PCOS mean age:(29.3?3.7)years,mean body mass index:(24.3?8.3)kg/m2] were enrolled in this study.Transvaginal hydrolaparoscopy using a F5 bipolar needle was performed on the patients to create 5-15 holes with a free length of 0.8 cm and a diameter of 0.2 mm in each ovary(electrocoagulation time 10-15 sec).Results A mean of(17?6)holes were made in both the ovaries in the patients.No surgical complications occurred.The levels of LH,FSH,and androstenedione dropped from(14.7?4.5)IU/L,(7.1?6.4)IU/L,and(12.2?4.4)nmol/L to(10.5?3.7)IU/L,(6.4?1.7)IU/L,and(8.9?3.0)nmol/L(t=2.389,P=0.027;t=1.007,P=0.326;and t=2.104,P=0.048)respectively after the operation.The patients were followed up for 2 to 9 months,during the period 6(54.5%)patients recovered spontaneous menstruation,among which 3 recovered ovulatory cycles(27.3%)and 2(18.2%)had spontaneous pregnancy.In the other 8 patients who didn't recover spontaneous menstruation,5 cases received CC or FSH(one of them was pregnant after the treatment);1 case was not pregnant though received IVF;and 2 are still in follow-up.Conclusions Transvaginal hydrolaparoscopic ovarian drilling is safe and effective for clomiphene citrate-resistant infertile women.

Objective To study the value of laparoscopic operation in the management of ectopic pregnancy. Methods The management of 509 cases of ectopic pregnancy reviewed retrospectively in our hospital from January 1999 to December 2001.Selected 60 cases treated by laparoscopy in 2001 were compared with 58 cases treated by laparotomy without hypovolemia shock.Operating time, postoperative hospital stay, time returning to normal temperature,total cost were compared. Results Laparoscopic surgery was performed in three cases in 1999,twenty three cases in 2000 and sixty cases in 2001. Comparison between laparoscopic and laparotomy group showed the operating time (66.3?25.3) min vs (75.9?22.0)min( t =-2.02, P =0.03);postoperative hospital stay (3.3?1.9)days vs (5.1?1.7)days( t =-5.42, P =0.00); total cost (7318.4?1440.1) RMB vs (5567.8?1567.8) RMB ( t =6.32, P =0.00). Conclutions Laparoscopic surgery is superior to laparotomy in the management of ectopic pregnancy.

Objective To investigate the effects of laparoscopic management of hydrosalpinx on outcomes of in vitro fertilization and embryo transfer(IVF-ET).Methods A total of 53 patients with hydrosalpinx undergoing IVF-ET from 2002 to 2005 were divided into three groups: ①Untreated Group included 17 patients who were given no interventions for hydrosalpinx prior to IVF-ET;②Preoperative Group included 17 patients undergoing laparoscopic treatment of hydrosalpinx prior to IVF-ET;③Postoperative Group had 19 patients who had experienced a history of unsuccessful IVF-ET treatment cycles and received laparoscopic management of hydrosalpinx prior to subsequent IVF-ET cycles.Results The pregnancy rates in fresh IVF cycles,the pregnancy rates in frozen cycles,the mean pregnancy rates per transfer,and the accumulative rate of live fetuses were 15.8%(3/19),10.5%(2/19),13.2%(5/38),and 17.6%(3/17) in the Untreated Group,36.8%(7/19),23.1%(3/13),30.3%(10/32),and 41.7%(8/17) in the Preoperative Group,and 16.7%(2/12),58.3%(14/24),44.4%(16/36),and 73.7%(14/19) in the Postoperative Group,respectively.The pregnancy rates in frozen cycles,the mean pregnancy rates per transfer,and the accumulative rate of live fetuses were all significantly higher in the Postoperative Group than in the Untreated Group(?~2=10.374,P=0.001;?~2=8.903,P=0.003;?~2=11.305,P=0.001,respectively).The mean pregnancy rates per transfer and the accumulative rate of live fetuses in the Preoperative Group tended to higher than those in the Untreated Group(?~2=3.377,P=0.066;?~2=3.360,P=0.067).Conclusions Hydrosalpinx is associated with poor IVF-ET outcomes.Laparoscopic management for hydrosalpinx prior to IVF-ET improves the pregnancy rate of IVF-ET.