Objective To investigate the effect and mechanism of puerarin on the proliferation,invasion and apop-tosis of breast cancer cell HCC1806 via network pharmacology,molecular docking and in vitro experiments.Meth-ods The data of breast cancer and mitochondrial diseases were collected from GEO database,and the differentially expressed genes were analyzed by GEO2R.Overlapping genes between breast cancer and mitochondrial database were analyzed by Venn diagram.GO and KEGG enrichment analyzed the overlapping genes.STRING and Cyto-scape analyzed overlapping gene interaction networks and screen core genes.Interaction between puerarin and core genes was docked with autodock.Cell proliferation,apoptosis and invasion capacity were measured by CCK-8,EdU,TUNEL and Transwell experiments,mitochondrial membrane potential was measured by Mito-Tracker experi-ments and protein expression levels were measured by Western blot.Anti-tumor efficacy of puerarin was analyzed by subcutaneous xenograft of breast cancer and immunohistochemical assay in vivo.Results Among 132 overlap-ping genes in breast cancer and mitochondrial disease,10 core differentially expressed genes were selected.Among these core genes,dual serine/threonine and tyrosine protein kinase(DST)was low expressed in breast cancer tis-sues.Puerarin bound to DST,up-regulated its expression,inhibited the proliferation and invasion of HCC1806 breast cancer cells,promoted breast cell apoptosis,increased the expression levels of apoptosis-related proteins Cleaved-Caspase 3 and Bax,down-regulated the expression of anti-apoptosis protein Bcl-2,and decreased mito-chondrial membrane potential.In vivo,puerarin inhibited the growth of breast cancer and up-regulated the expres-sion of DST in tumor tissues.Conclusion Puerarin inhibits the proliferation and invasion of breast cancer cells,promotes the apoptosis of cancer cells and inhibits breast cancer growth.

Objective To introduce the modeling method of pendulum-like modified rat abdominal heart heterotopic transplantation model and evaluate the quality of the model. Methods An operator without transplantation experience performed 15 consecutive models, recorded the time of each step, changes in body weight and modified Stanford scores, and calculated the surgical success rate, postoperative 1-week survival rate and technical success rate. Ultrasound examinations was performed in 1 week postoperatively. Results The times for donor heart acquisition, donor heart processing, recipient preparation and transplantation anastomosis were (14.3±1.4) min, (3.5±0.6) min, (13.6±2.1) min and (38.3±5.2) min respectively. The surgical success rate was 87% (13/15), and the survival rate 1 week after operative was 100% (13/13). The improved Stanford score indicated a technical success rate of 92% (12/13), and the postoperative 1-week ultrasound examination showed that grafts with Stanford scores ≥3 had detectable pulsation and blood flow signals. Conclusions The pendulum-like modified rat abdominal heart heterotopic transplantation improved model further optimizes the operational steps with a high success rate and stable quality, may be chosen as a modeling option for basic research in heart transplantation in the future.