OBJECTIVETo evaluate the safety and effectiveness of a modified method of holmium laser enucleation of the prostate (HoLEP)--6-o'clock tunnel HoLEP for the treatment of benign prostate hyperplasia (BPH).

METHODSWe included 112 cases of BPH in this study, 57 treated by 6-o'clock tunnel HoLEP (experimental group) and the other 55 by conventional HoLEP (control group). We compared the operation time, volume of the resected prostatic tissue, intraoperative blood transfusion, volume of bladder irrigation solution, postoperative hemoglobin change, and incidence of urinary incontinence between the two groups.

RESULTSStatistically significant differences were observed between the experimental and control groups in the operation time ([56.01 ± 8.62] min vs [68.65 ± 9.08] min), cases of intraoperative blood transfusion (0 vs 2), volume of bladder irrigation solution ([27.51 ± 3.67] L vs [36.89 ± 6.47] L), postoperative hemoglobin decrease ([10.70 ± 2.50] g/L vs [12.60 ± 3.30] g/L), and cases of postoperative stress-induced urinary incontinence (2 vs 7) (all P <0.05). One-month follow-up revealed smooth urination in both groups of patients but no true urinary incontinence or secondary bleeding in either.

CONCLUSIONModified 6-o'clock tunnel HoLEP can significantly reduce the operation time, bladder irrigation, and intraoperative bleeding, and therefore can be used as a safe and effective option for the treatment of BPH.

Case-Control Studies ; Hemorrhage ; prevention & control ; Holmium ; Humans ; Laser Therapy ; methods ; Lasers, Solid-State ; therapeutic use ; Male ; Operative Time ; Postoperative Period ; Prostatic Hyperplasia ; surgery ; Therapeutic Irrigation ; statistics & numerical data ; Urinary Bladder ; Urinary Incontinence ; epidemiology ; etiology ; Urinary Incontinence, Stress ; etiology

Objective To evaluate the feasibility of 3-(4-~(18)F-fluorobenzyl)-8,9-dimethoxy-1,2,3,4-tetrahydrochromeno [3,4-c]pyridin-5-one ( is F-FDTP) as a potential dopamine D4 receptor PET imaging agent.Methods ~(18)F-FDTP solution in ethanol-physiological saline was incubated with calf serum to test its in vitro stability through the determination of radiochemical purity.Normal rats were injected intravenously with ~(18)F-FDTP and then sacrificed at 2,5,10,15,30,60 and 120 min after anesthesia.Blood,organs and brain tissue samples were collected.All samples were weighed and measured for radioactivity.The uptake of samples was expressed as percentage activity of injection dose per gram of tissue ( % ID/g).Results The stability of ~(18)F-FDTP was satisfactory and its radiochemical purity was above 95% after incubation 120 min at 37℃ in calf serum.The biodistribution showed that ~(18)F-FDTP could penetrate through the blood-brain barrier and selectively accumulate in striatum,hypothalamus,frontal certex,hippocampus,cerebellum,where the D_4 receptor was reportedly located.The radioactivities in hippocampus,hypothalamus,striatum,frontal cortex,cerebellum,pons were (0.42±0.03),(0.46±0.05),(0.54±0.04),(0.39±0.04),(0.45±0.06),(0.35±0.04) %ID/g,respectively,2 min post injection.And there was difference between the normal biodistribution results and the blocking experimental results:(0.36 ±0.05),( 0.33±0.05 ),(0.55±0.05 ),(0.30±0.07 ),(0.34±0.07 ) and (0.32±0.04) % ID/g in hippocampus,hypothalamus,striatum,frontal cortex,cerebellum and pons,respectively.Conclusions ~(18)F-FDTP can penetrate through the blood-brain barrier and selectively accumulate in striatum,hypothalamus,frontal cortex,hippocampus,cerebellum,where the D_4 receptor was known to concentrate.These preliminary results suggest that ~(18)F-FDTP is a potential dopamine D_4 receptor imaging agent and further studies are needed.

OBJECTIVETo investigate the rehabilitation effect of electroacupuncture (EA) at early stage on hemiplegia in the postoperative patient of hypertensive hemorrhage.

METHODSSeventy-two cases of postoperative patient of hypertensive hemorrhage were divided into an EA group (n = 42) treated with EA at Quchi (LI 11), Shousanli (LI 10), Hegu (LI 4), Zusanli (ST 36), etc., from 1-3 days after operation, twice each day; and a control group (n = 30) with functional exercises. Their rehabilitation effects were compared after treatment of one month.

RESULTSThe rehabilitation effect in the EA group was significant better than that in the control group (P < 0.01).

CONCLUSIONEA at early stage has good recovery effect for the patient of hemiplegia after operation of hypertensive hemorrhage.

Acupuncture Points ; Electroacupuncture ; Hemorrhage ; Humans ; Hypertension ; Postoperative Period

OBJECTIVETo investigate the influence of early enteral nutrition on the nutritional indices of the burn patients.

METHODSThirty-seven burn patients were enrolled in the study and were randomly divided into early enteral nutrition (EN) and parenteral nutrition (PN) groups. The body weight, serum levels of prealbumin and transferrin, the incidence of burn sepsis, and the hospital stay days were surveyed respectively and compared between these two groups.

RESULTSThe percentage of body weight loss in EN group was obviously lower than that in PN group on the 7th and 14th post burn day (PBD) (P < 0.05). The serum contents of prealbumin and transferrin in EN group were significantly higher than that in PN group on the 4th, 8th and 14th PBD (P < 0.05 or 0.01). The incidence of burn sepsis in EN group was lower than that in PN group (5.56% vs 31.58%, P < 0.05). The hospital stay days in EN group were less than that in PN group (40.39 +/- 10.81 d vs 54.89 +/- 11.26 d, P < 0.01).

CONCLUSIONEarly enteral nutrition is beneficial to the improving of nutrition state of the burn patients, and to the reduction of the incidence of burn sepsis and the hospitalization time.

Adult ; Burns ; blood ; therapy ; Enteral Nutrition ; Female ; Humans ; Male ; Parenteral Nutrition ; Prealbumin ; metabolism ; Sepsis ; prevention & control ; Serum Albumin ; Time Factors ; Transferrin ; metabolism ; Young Adult

The characteristics of purinoceptors in the membrane of rat trigeminal ganglion (TG) neurons were studied by using whole- cell patch clamp technique. The results showed that most of neurons examined (78.9%, 142/180) were responsive to ATP in a concentration-dependent manner; the others (21.1%, 38/180) were ATP insensitive. Of the ATP-sensitive cells, the majority (95.1%, 135/142) responded to ATP with an inward current, a few (2.1%, 3/142) with an outward current, and the rest (2.8%, 4/142) with biphasic current. Small sized cells (<30 mum) responded to ATP with a rapid desensitizing inward current and were highly sensitive to vanilloid; the medium sized cells (30~40 mum) responded to ATP with slow desensitizing inward current and were not sensitive to vanilloid; while the majority of large sized cells (>40 mum) did not respond to ATP and vanilloid. The waveform of ATP-activated inward currents was related to the cell diameter. The I-V curves for both small and medium sized cells manifested obvious inward rectification. Furthermore, we studied the kinetic features of ATP-activated currents and the effects of P2 purinoceptor agonists and antagonists on I(ATP). The findings suggest that ATP receptor-ion channels are expressed differently among different types of rat TG neurons.
Adenosine Triphosphate ; metabolism ; Animals ; Animals, Newborn ; Neurons ; metabolism ; physiology ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley ; Receptors, Purinergic P2X ; physiology ; Trigeminal Ganglion ; cytology ; metabolism ; physiology

15 scores were randomly divided into therapeutic group (30 cases)and control group(30 cases).The two groups were treated with the same conventional therapy. From the 1st day of admission,Xuebijing injection 80 ml was given by intravenous drip once for every 12 hours for 7 days to the therapeutic group.The level of tumor necrosis factor-?(TNF-?),interleukin-1?(IL-1?),IL-6,IL-10,and APACHEⅡscore were tested on 1,3,5 and 7 days after admission.The case fatality and incidences rates of sepsis and MODS in both groups were recorded.Results Compared with the control group,APACHEⅡscore,TNF-?,IL-1?,IL-6 and IL-10 of the therapeutic group were lower significantly on the 3rd day after admission,especially on the 5th day(all P

Objective To explore the clinical efficacy and safety of milrinone combined with low-dose digoxin for treatment of chronic pul-monary heart disease.Methods A total of 60 patients suffering from chronic cor pulmonale disease ( COPD ) with heart failure were rando-mized into two groups.The control group( n=30) was treated with low-dose digoxin to control heart failure.The treatment group ( n=30 ) was treated with milrinone plus low -dose digoxin to control heart failure. Other treatments were similar.Results In improvement of cardiac func-tion, the total efficiencies of control group and treatment group were 73.33%and 93.33%, respectively, there was a marked difference be-tween the two groups (P<0.05).After treatment, the alveolar oxygen partial pressure(PaO2)of two groups increased significantly (P<0.05), while the heart rate, alveolar partial pressure of carbon dioxide ( PaCO2 ) and pulmonary artery pressure decreased significantly(P<0.05)had sta-tistical significance between two groups ( P<0.01) , the above indicators of treatment group were better than those of control group. Conclusion The regimen of milrinone plus low-dose digoxin is effec-tive in controlling heart failure of patients with chronic cor pulmonale. Hence, it is worthy of being recommended in clinical practice.

Objective To study the effect of music intervention through supra-aural earphone connected to MP3 on MAP, HR, RR and SpO2 of patients during cardiac interventional therapy.Methods 60 patients undergone cardiac intervention were randomly divided into control group (30cases) and experimental group (30 cases) according to operation time order. During the operation, traditional care was applied in control group and music intervention on the basis of traditional care was applied in experimental group. The changes of MAP, HR, RR and SpO2 and breathing mechanics were observed during four periods: 3 days before operation(T0), enter the operating room and anesthetic efficacy was definite and without music(T1), the first 11 min of operation (T2), and from the 11th min to the end of the operation (T3).Results In the control group, as compared to T0, MAP increased in T1, T2 and T3, the difference was statistically significant [the values were (105.7±10.8),(123.5±17.8),(125.1±18.5),(110.9±10.5)mm Hg, respectively, F=5.11, P＜0.05]. The values of HR and RR markedly increased in T1, T2 and T3 compared with T0 and the differences were statistically significant (P＜0.05). The values of MAP and HR also went up in T1 and T2 in experimental group, but the increase amplitude in T2 was obviously lower than that in T1 and that in control group, and the values in T3 nearly returned to the level in T0. Significant differences were found in MAP of T2 [(125.1±18.5)mm Hg vs (114.1±15.6)mm Hg,t=2.55,P＜0.05] and T3 as well(P＜0.05). Compared with themselves in each period, the values of SpO2 in the two groups had no significant differences(P>0.05). However, 3 patients' value of SpO2 decreased to 80% in control group on the account of tense breath holding. On the contrary, the respiration of patients in experimental group was much steadier. There was statistical difference between the two groups(χ2=2.73,P ＜0.05).Conclusions Application of music intervention in cardiac interventional therapy may degrade the stimulus of iatrogenic noise and maintain the equability of MAP, HR, RR and SpO2, and thus promote medical care quality.

OBJECTIVETo evaluate the clinical effects of close reduction combined with minimally invasive percutanous plate osteosynthesis (MIPPO) for proximal and distal tibial fractures.

METHODSFrom March 2007 to December 2010, 56 patients with proximal and distal tibial fractures were treated with close reduction combined with MIPPO technique. There were 39 males and 17 females,aged from 22 to 67 years with an average of 41.3 years. Left fracture was in 25 cases and right fracture was in 31 cases; proximal tibial fracture was in 15 cases and distal tibial fractures was in 41 cases; 34 cases caused by fall down and 22 cases caused by road accident. The mean time from injury to operation was 1.7 d. Clinical manifestation included pain, swelling of leg with limitation of activity. According to the standard of Johner-Wruhs, clinical effects were evaluated.

RESULTSThe mean operative time was 46 min in 56 patients. All fractures obtained satisfactory reduction and the location of plate was good. Incisions healed with one-stage and no superficial or deep infection was found. All the patients were followed up from 8 to 23 months with an average of 14.2 months. Only one fracture complication with delayed union,and after auto grafting with ilium bone,the fracture got union. Other 55 cases obtained bone healing in 15 to 20 weeks after operation and no internal fixation failure was found. The time of walking was 4-6 months after operation,without limping at 7 months after operation. Both lower extremities were symmetrical and the function of knee and ankle got complete recovery. According to the criteria of Johner-Wruhs score,46 cases obtained excellent results,9 good and 2 fair.

CONCLUSIONTreatment of proximal and distal tibial fractures with close reduction and MIPPO technique can not only preserve soft tissue,simplify operative procedure and decrease wound, but also can obtain rigid internal fixation and guarantee early function exercises of knee and ankle joints. The method has the advantages of less soft tissue injury, less blood loss, reliable fixation, which is effective method in treating proximal and distal tibial fractures and corresponds with the standpoint of biological fixation.

Adult ; Aged ; Bone Plates ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; methods ; Tibial Fractures ; surgery

OBJECTIVETo clone the mouse testis specific gene TSEG-2 via a bioinformatic approach.

METHODSThe expressed sequence tags (EST) in the normal mouse testis were obtained from the online EST database ZooDDD. Their highly homologous EST sequences were retrieved through the dbEST database to construct contigs and spliced with the biomedical software Biolign. The corresponding exons and introns within the genome sequences were predicted with the software GeneScan. Primers were designed according to the open reading frame. RT-PCR was applied in cloning the cDNA of the novel gene from the mouse testis tissue and analyzing its expression patterns in the undescended testis and various organ tissues as well as in different developmental stages of the mouse testis. The sequencing results of TSEG-2 underwent bioinformatic analyses.

RESULTSThe novel mouse testis gene TSEG-2 was successfully cloned, with full-length sequence of 451 bp. The open reading frame was 267 bp, coding a protein of 88 amino acid residues, and demonstrated to be correct by RT-PCR. The expression of TSEG-2 was high in the mouse testis, regular in the testis cDNA samples of different postnatal days, and down-regulated in the cryptorchidism model. No obvious homology with other mouse cDNA was found for TSEG-2. The GenBank accession number EU079025 was achieved. Function prediction showed that mouse TSEG-2 was probably a soluble non-secretary protein located at chromosome 15qE3, or a nucleoprotein with 2 phosphorylation sites of protein kinase C (PKC) and 1 of casein kinase II (CK2).

CONCLUSIONA novel mouse testis specific gene TSEG-2 was successfully cloned, which could be down-regulated by cryptorchidism-inducible 17-beta estradiol. This has prepared the ground for further researches on the biological function and expression regulation of TSEG-2.

Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Expressed Sequence Tags ; Female ; Gene Expression ; Male ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; Open Reading Frames ; Pregnancy ; Proteins ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Testis ; metabolism