Objective To assess the effect of atorvastatin on the Lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2) expression in cultured human pulmonary epithelial cells (A549). Methods A549 cells were incubated in the medium containing LPS and different concentrations of atorvastatin(0,10,15,20?M/ml, respectively) for 12h. Then the total cellular RNA and proteins from the cells treated with different experimental conditions were extracted for RT-PCR and western blot analysis,respectively. Results In cultured human pulmonary epithelial cells, atorvastatin reduced the expression of COX-2 mRNA and protein induced by LPS in a dose-dependent manner. Conclusion Atorvastatin may down-regulate LPS-induced COX-2 expression in cultured human pulmonary epithelial cells.

Objective The present study was undertaken to investigate the properties of nonlinear dynamics of EEG and the changes in depth of anesthesia with real-time approximate entropy (ApEn) and complexity (Cx) nonlinear indexes monitoring during anesthesia. Methods EEG was recorded in 65 in-patients. They were randomly divided into 4 groups: isoflurane, sevoflurane, desflurane (n=15, respectively), and propofol intravenous anesthesia (n=20) groups. The EEG derived parameters ApEn and Cx non-linear indexes were calculated simultaneously during the whole operation including rest state with eyes closed, anesthetic induction, intraoperation, recovery, post-operation awaking. Results ApEn and Cx nonlinear indexes remained the highest during rest state. Both of them kept decreasing during anesthetic induction. They dropped to a relative lower value and leveled off in the intra-operation period. Both of them rose gradually during recovery period and returned to a high level in the post-operation awaking period (correspondingly, ApEn: 0.87, 0.78, 0.55, 0.64 and 0.83. Cx: 0.58, 0.54, 0.38, 0.46 and 0.57). Conclusions With ApEn and Cx non-linear indexes, changes in depth of anesthesia from EEG signal could be real-timely monitored and precisely measured. Nonlinear dynamic analysis might provide us with more information about consciousness and cognition during general anesthesia.

Objective To investigate the dietary structure,nutrition and health status of elderly military cadres.Methods Questionnaire,dietary investigation and physical examination were performed in elderly cadres in Hangzhou Sanitarium in 1992,2002 and 2008(520,720 and 643 cases,respectively).The dietary structure,nutrition and health status were analyzed systematically.Results The intake of cereals dropped,and also the dried legumes.The intake of meats and aquatic products was significantly increased,and the intake of all the vegetables,fruits,dairy products,nuts and vegetable oils was rapidly increased too.The intake of pickles was decreased.The intake of salt and soy sauce showed a insignificant tendency of change.The intake of food supplying energy and protein had exceeded the recommended nutrient intake(RNI).The intake of niacin,vitamin E and Fe was increased gradually and reached the standard of RNI.Vitamin B1 intake also reached that of RNI.Vitamin C intake was increased rapidly,and reached 176.0?23.5mg/d in 2008.Zinc intake was not enough in 1992,while reached the standard of RNI in 2002 and 2008.Selenium intake reached RNI in 2008.With the increasing of milk consumption,calcium intake had greatly improved.There was also an increase in the proportion of energy from fat,which reached 37% in 2008.The health status of military old cadres had greatly improved.The prevalence of digestive diseases and hyperosteogeny decreased significantly by years,while the cardiovascular and cerebrovascular diseases increased.Conclusions The nutrition status and the quality of average diet in military elderly cadres have improved obviously,while the intake of fat,cholesterol and sodium is excessive.As a result,overweight,obesity and hypertension are prevalent.Propagation of nutrition knowledge and nutrition intervention should be emphasized.

Objective To investigate effect of mild hypothermia on changes of somatosensory evoked potential (SEP) and synaptophysin mRNA level after traumatic brain injury (TBI) and determine hypothermia-induced neuroprotection.Methods Forty-five SD rats were allocated into mild hypothermia group,TBI group and sham operation group with 15 rats per group according to the random number table.Left-side fluid percussion impact was performed to induce models of TBI.Rats were exposed to hypothermia environment (32-35℃) for 6 hours in mild hypothermia group after TBI.Rats in sham operation group were treated by only drilling on left side of the head,rather than hitting.To evaluate function outcome,modified neurological severity score (mNSS),SEP and synaptophysin mRNA level were measured at 6 hours,24 hours and 7 days postinjury.Results The mNSS in mild hypothermia group lowered compared with TBI group,especially at 24 hours and 7 days (P ＜ 0.05).SEP in mild hypothermia group was significantly shortened at 6 and 24 hours compared with TBI group (P ＜ 0.05),but SEP revealed no significant difference among the 3 groups at 7 days (P ＞ 0.05).Level of synaptophysin mRNA in mild hypothermia group increased at 6 hours postinjury compared with TBI group [(0.08 ± 0.02) vs (0.12 ±0.04)],with further increase at 7 days postinjury[(0.06 ± 0.01) vs (0.33 ± 0.10)] (P ＜0.05).Conclusion The shortage of nerve conduction time of the injured side and promotion of nerve regeneration suggest the neuroprotective role of mild hypothermia following TBI.

Adult ; Asthma, Occupational ; therapy ; Bronchoalveolar Lavage ; Humans ; Male ; Textiles ; adverse effects

Purpose To investigate the clinicopathologic features, diagnosis and differential diagnosis of ovarian juvenile granulosa cell tumor (JGCT).Methods The history records, pathologic features and immunophenotype of 8 cases of JGCT were retrospectively evaluated and their prognosis was achieved by follow-up.Results The age of patients ranged from 6～21 years old,with an average age of 15.1 years.The main clinical manifestations included an abdominal mass, ascites and isosexual pseudoprecocity. Cut surface of the tumor was typically solid with cysts formed. The histopathological changes displayed solid nests, diffuse sheet, multiple round or ovoid follicles in variable size.Macrofollicles could be seen in some cases.The follicular pattern consisted of small cystic cavities containing eosinophilic secretions. The tumor cells were round or polygonal, medium in size. The tumor cells had abundant pale or slightly eosinophilic cytoplasm, round nuclei with fine chromatin. Nuclear grooves were inconspicuous.Mitosis figures could be found. Immunohistochemical results showed that the tumor cells expressed inhibin-α,CD99,vimentin; while Melan-A,calretinin and S-100 were positive staining in part of the cases.CKpan,EMA,PLAP,Syn and CgA were negative in all the cases.Conclusions Ovarian juvenile granulosa cell tumor is a rather rare, low malignant tumor with good prognosis. Its diagnosis depends on the histologic and immunohistochemical findings and clinical features. Its differential diagnosis includes adult granulose cell tumor, hypercalcaemic type small cell carcinoma, carcinoid and dysgerminoma.

Animals ; Chemistry Techniques, Analytical ; methods ; Food Analysis ; Humans ; Pharmaceutical Preparations ; analysis

Objective To stuay CT findings of adrenal gland trauma(AGT).Methods The CT images of adrenal gland trauma which were found in 1712 patients who had emergency abdominal CT examinations for assessment of blunt force trauma were analyzed restrospectively.Results Among 1712 cases.we identified 29 patients(1.7%)with adrenal gland traumatic lesions.A total of 32 lesions were found.Twenty-six lesions were unilateral(23 right-sided,3 left-sided),and 3 cases were bilateral(6 lesions).Main CT findings were as followed:(1)Adrenal hematomas appeared as discrete round or oval masses expanding the adrenal gland in 22 patients with 25 lesions.The lesions were hyperdense or of homogeneous density in plain scan and did not enhance in three-phase contrast-enhanced dynamic scan.The splayed adrenal limbs around hematoma were enhanced significantly as eurvilinear structures.(2)Mild to moderate uniform swelling of gland was shown in 4 cases.(3)Diffuse irregular hemorrhage obliterating the gland was shown in 3 cases.Contrast extravasation was shown in 1 case,which was active bleeding from the broken adrenal gland proven by operation.Associated CT findings included strand-like hemorrhage of the periadrenal fat and posterior pararenal hemorrhage mimicking thickened diaphragmatic crus.The concurrent injuries of ipsilateral thorax or/and abdomen were found in 25 patients.Conclusion The AGT has typical CT findings.Familiarity with characteristic CT findings of adrenal trauma is essential for the radiologist to avoid misdiagnosis.

Objective To study the expressions of erythmpoietin(EPO)and its receptors(EPOR)in the injured brain tissue ofthe rats with traumatic brain injury(TBI).Methods A total of78 SD rats were randomly divided into three groups including control group(six rats),sham group(36rats) and fluid percussion injury group(36 rats).The rats were sacrificed at 6,24 hours,3,5,7 and 14days after TBI in the sham group and the fluid percussion injury group(six rats at each time point).Then,the injured brain tissues were removed for observation of the mRNA and protein expressions of EPO and EPOR by meaDiB of real-time PCR and Western blot. Results The expression of EPO was increased at 24 hours and reached the peak at day 3 after TBI.The hish expression level of EPO could maintain for two days or so.began to decrease at day 7 and recovered to normal at day 14 after Till.While the expression of EPOR reached the peak at 24 hours after TBI and maintained hish level at day14. Conclusions The expressions of EPO and EPOR show increase within 24 hours after TBI.In fact,the expressions of both factors are not in consistency,with more transient expression of EPO.

Objective To assess the clinical outcomes of glutamine supplementation in neonates.Methods The study was designed as a double-blind randomized controlled trial.Totally 100 infants were randomly divided into 5 groups with 20 infants in each group:control group:standard parenteral nutrition (PN);PNI group:PN supplemented with 0.3 g/ (kg·d) glutamine;enteral nutrition (EN) 1 group:EN supplemented with 0.3 g/ (kg·d) glutamine;PN2 group:PN supplemented with 0.3 g/ (kg·d) glutamine;and EN2 group:EN supplemented with 0.3 g/ (kg·d) glutamine,The primary endpoints were the time to establish full enteral nutrition [defined as receiving 120 ml/ (kg·d) enterally],episodes of gastric residuals,total duration of PN,and mortality.The second endpoints were weight gain and head circumference gain,liver function,renal function,days on ventilator,and length of hospitalization.Results Time to achieve full enteral nutrition,incidence of gastric residuals,duration of PN,weight gain (g/d) ,head circumference gain (cm/w),liver function,renal function,and length of hospitalization were not significantly different among all five groups.Days of mechanical ventilation were significantly decreased in PN1 group and PN2 group when compared with the control group (P ＜ 0.05).Intention-totreat analyses showed that,when compared with the control group,PN1 group:RR:1.053;95% CI:0.952-1.164;ENI group:RR:1.333,95% CI:1.035-1.717;PN2 group:RR:1.053,95% CI:0.952-1.164;EN2 group:RR:1.25,95% CI:1.004-1.556.Conclusions Glutamine supplementation may not be able to decrease the time to achieve full enteral nutrition,the incidence of gastric residuals,and the duration of PN.However,pareneral glutamine supplementation does reduce the days on ventilator in neonates.Also,parenteral glutamine supplementation has no obvious influence on mortality.