2.Protecting effect of Cistanche extracts on MPP+-induced injury of the Parkinson's disease cell model
Hu WANG ; Wenwei LI ; Dingfang CAI ; Ru YANG
Journal of Integrative Medicine 2007;5(4):407-11
OBJECTIVE: To investigate the potential protective role of Cistanche extracts on 1-methyl-4-phenylpyridinium ion (MPP(+))-induced Parkinson's disease (PD) cellular model, and to find out whether this effect is achieved through the regulation of growth arrest- and DNA damage-induced gene 153 (GADD153). METHODS: MPP(+))-induced cellular injury and the protective effect of Cistanche extracts on the SH-SY5Y cell line viability treated by MPP(+)) were investigated by using methyl thiazolyl tetrazolium (MTT) assay. The mRNA of GADD153 in SH-SY5Y cell line treated by MPP(+)) and Cistanche extracts were evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). The protein level of GADD153 in SH-SY5Y was assessed by Western blotting. RESULTS: Cistanche extracts (100 mug/ml) increased the cell viability (P<0.01). And the mRNA of GADD153 in the Cistanche extracts pretreatment group was much less than that in the MPP(+)) group (P<0.01). The result of Western blotting showed that GADD153 had a lower level in the Cistanche extracts pretreatment group, compared with MPP(+)) group, especially in the 100 microg/ml group (P<0.01). CONCLUSION: Cistanche extracts pretreatment has a protective effect on the MPP(+))-treated SH-SY5Y cell line, and its down-regulation of GADD153 may contribute to the effect.
3.Leiomyosarcoma of mediastinum: report of a cases.
Chang-ping LI ; Ya-ru WANG ; Jun-cai ZHOU
Chinese Journal of Pathology 2007;36(1):58-58
Actins
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metabolism
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Adult
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Desmin
;
metabolism
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Diagnosis, Differential
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Histiocytoma, Malignant Fibrous
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pathology
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Humans
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Immunohistochemistry
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Leiomyoma
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pathology
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Leiomyosarcoma
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metabolism
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pathology
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surgery
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Male
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Mediastinal Neoplasms
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metabolism
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pathology
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surgery
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Vimentin
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metabolism
4.Optimization of Extraction Process of Flavonoids from Toosendan Fructus by Response Surface Methodology
Yongzhi TIAN ; Yali WANG ; Ru SUN ; Jing ZHAO ; Qun LIU ; Chenxiao WANG ; Yan MA ; Meichao CAI
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(4):76-79
Objective To optimize extraction process of flavonoids from Toosendan Fructus.Methods Three parameters that significantly affect the extraction rate of flavonoids, ratio of material to water, extraction time and ethanol concentration, were taken into consideration for extraction ratio of flavonoids from Toosendan Fructus. Based on the results of single-factor experiment, the extraction parameters of flavonoids from Toosendan Fructus were optimized by Box-Behnken of response surface methodology (RSM).Results The optimum extraction conditions were as follow: material to water ratio was 1:35; extraction time should be 3 h; ethanol concentration was 70%. The extraction ratio was up to 0.587 3% in the optimum extraction conditions.Conclusion The actual value is highly matching the theoretically value of RSM model. The results indicated that this method can rationally optimize extraction process of flavonoids from Toosendan Fructus.
5.Influence of luyoutal on serum cytokines in patients with depression.
Wei CAI ; Zhan-kun WANG ; Shu-ru HUANG
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(11):864-865
Adult
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Antidepressive Agents
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therapeutic use
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Bipolar Disorder
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blood
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drug therapy
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Humans
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Interferon-gamma
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blood
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Interleukin-1
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blood
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Interleukin-6
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blood
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Male
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Middle Aged
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Perylene
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analogs & derivatives
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therapeutic use
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Phytotherapy
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Tumor Necrosis Factor-alpha
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metabolism
6.Two case reports of scrotal skin burn complicated with organ function lesion caused by paraquat.
Guang-cai YU ; Xiang-dong JIAN ; Jie-ru WANG ; Jing SUN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(11):865-866
Adult
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Burns
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etiology
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Humans
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Male
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Middle Aged
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Paraquat
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adverse effects
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Scrotum
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injuries
7.Effect of Early Rehabilitation on Neurological Function and Cerebral Blood Flow of Patients with Acute Cerebral Infarction
Zhao-ru GAN ; Qing-wu WANG ; Hai-lan LIU ; Chaoqun CAI ; Wenxin YANG
Chinese Journal of Rehabilitation Theory and Practice 2008;14(12):1163-1164
Objective To investigate the effect of early rehabilitation on neurological function and cerebral blood flow of patients with acute cerebral infarction.Methods100 cases of acute cerebral infarction were randomly divided into the rehabilitation group(53 cases) and control group(47 cases).Both groups received the routine treatment;besides,patients in the rehabilitation group were treated with early rehabilitation training.The treatment efficacy was assessed by neurological function deficit evaluation,and the cerebral blood flow was measured by transcranial Doppler(TCD) before and 30 days after treatment.ResultsThe scores of neurological function deficit evaluation of all patients in each group decreased after treatment,but the scores of the rehabilitation group were obviously lower than that of the control group(P<0.01);and the results of TCD of the rehabilitation group were also superior to that of the control group(P<0.01).ConclusionEarly rehabilitation training may obviously improve neurological function and cerebral blood flow of patients with acute cerebral infarction.
8.Investigation on anti-hepatitis B virus active components and mechanism of Salvia miltiorrhiza based on network pharmacology
Hai-mei WEN ; Si-yuan WANG ; Ya-ru WANG ; Feng-li XING ; Yong CHEN ; Wen-tao CAI
Acta Pharmaceutica Sinica 2022;57(5):1375-1386
We predicted the anti-hepatitis B virus (HBV) active components and mechanism of
9.Clinical significance of detecting CXC chemotatic factor in early diabetic retinopathy
Hong, ZHU ; Hai-lin, HU ; Meng-ru, SU ; Yao-chun, ZHU ; Wen-qiu, WANG ; Cai-hong, SHI ; Xiao-dong, SUN
Chinese Journal of Experimental Ophthalmology 2012;30(2):146-149
BackgroundDiabetic retinopathy (DR) is the result of the cytokine network disorders,the imbalance of angiogenic factor and vascular inhibitory factor is the start factor.ObjectiveTo analyze the levels of CXC chemotatic factors of type 2 diabetes mellitus patients,evaluate the clinical application value of them in different clinical types of DR using receiver operating characteristic (ROC)analysis and to approach the new way of individualized treatment.Methods This was a prospective research.The gold standard was ophthalmolscope and fundus fluorescein angiography.The levels of CXC chemotatic factors and multiplicaiton factors were measured in 96 cases with type 2 diabetes mellitus (66 cases with retinopathy and 30 cases without retinopathy as control).The assessment tasks were performed for these index and courses of DR with ROC curve.Results The expression of age,course of disease has significant difference in different courses of DR ( F =8.507,P =0.001 ; F =28.143,P =0.000).Compared with the control group,the expression of growth-related oncogene-α ( GROα ) ( t =- 2.172,P =0.035,AUC =0.625 ),whole blood viscosity 200 ( t =- 3.724,P =0.001,AUC =0.904 ) and neutrophilic leukocyte (t=-2.562,P =0.013,AUC =0.577 ) has significant difference in the group of mild NPDR.Compared with the control group,the expression of interferon-γ-inducible protein 10 ( IP-10 ) ( t =-3.591,P =0.001,AUC =0.592 ),platelet derivation growth factor-BB ( PDGF-BB ) ( t =- 3.233,P =0.003,AUC =0.735 ),vascular endothelial growth factor(VEGF) ( t =- 3.617,P =0.001,AUC =0.776 ),C peptide ( t =- 3.366,P =0.002,AUC =0.962 ),leukocyte ( t=-3.201,P =0.003,AUC =0.852) and neutrophilic leukocyte(t =-4.201,P=0.000,AUC =0.852) has significant difference in the group of moderate and severe NPDR.ConclusionsCXC chemotatic factors may act as reactivator in the pathogenesis of DR,GROα and IP-10 may be useful for clinical monitoring of the severity of DR,and evaluating the imbalance state of chemotatic factors maybe a new approach to clinical monitoring and prognosis of DR.
10.3 cases of paraquat poisoning inhalation.
Jie-ru WANG ; Xiang-dong JIAN ; Guang-cai YU ; Jing SUN ; Cheng-zhen SONG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(11):855-856
Adult
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Female
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Humans
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Inhalation Exposure
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Male
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Middle Aged
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Paraquat
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poisoning