OBJECTIVE:To provide reference for rational application of TCM injection. METHODS:The utilization of TCM injection in our hospital during 2011-2014 was analyzed in terms of species,consumption sum,DDDs and DDC,etc. RESULTS:The type and consumption sum of TCM injection kept stable in our hospital during 2011-2014. TCM injection for activating blood circulation to dissipate blood stasis as Shuxuetong(Heilougjiang),Danhong,Shuxuening took up the front of consumption sum in 4 years. Top 3 TCM injections in the list of DDDs were Shuxuetong,Shuxuening(Heilougjiang), Danhong/Xueshuangtong corre-sponded to consumption sum. DDC of TCM injection were kept around 100 yuan. DUI ratio of TCM injection were almost equal to 1 or close to 1. CONCLUSIONS:The amount and DDDs of TCM injection are synchronous in our hospital,and the utilization of TCM injection is stable and rational basically in our hospital. TCM injection with suitable price and definite efficacy take up a large share,and the amount of TCM injection for clearing away heat and toxic material with anti-inflammatory effect increase rapidly.

Objective To analyze the corneal flap thickness in laser in situ keratomileusis(LASIK) using Moria M2 microkeratome and to identify the related factors. Methods Sixty patients with LASIK were divided into two groups: M2 90 group,using the Moria M2 90 microkeratome,n=30;M2 110 group,using the Moria M2 110 microkeratome,n=30.All were performed on both eyes with the right one treated first.Subtraction pachymetry was used to measure corneal flap thickness which was analyzed statistically with the data including age,preoperative corneal diameter,curvature,corneal thickness and refraction. Results In the 30 patients of M2 90 group,the mean corneal flap thickness of right eye and left eyes were(128.03?12.03)?m(105~156 ?m) and(123.40?12.38) ?m(92~147 ?m),respectively,and the corneal flap thickness were statistically different between the right and left eyes(P

Inflammatory bowel disease ( IBD ) is a group of chronic non-specific inflammatory conditions of the intestine with unknown etiology.IBD has variety of intestinal manifestations and complications and there is lack of gold standard for diagnosis, so the disease should be diagnosed on the basis of clinical manifestations, endoscopic findings and histopathological examination.Most patients require surgical treatment and long-term maintenance of medication.Multi-disciplinary team (MDT), consisting of gastroenterologist, general surgeon, anorectal surgeon, nutritionist and psychologist, is needed for individualized treatment of patients.This article introduces the MDT model of diagnosis and treatment, and its application in inflammatory bowel disease.

Digestive diseases, are various types of frequently encounted clinical disorders .at present, diagnosis and differential diagnosis of digestive diseases , in addition to laboratory tests (biochemistry, immunology, cytology and bacterial genetics , etc.), to a large extent still rely on imaging studies.Flow cytometry is a function of the level of single cells or other biological particles for quantitative analysis and sorting means of detection , which can be tens of thousands of high -speed analysis of cells and can simultaneously from a cell a plurality of measured parameters , high speed, high precision, accuracy, etc., become the most advanced cellular quantitative analysis techniques .In recent years, flow cytometry is widely used in the diagnosis and treatment of gastrointestinal cancer , liver cirrhosis, Crohn′s disease, ulcerative colitis and other digestive diseases.

Objective To investigate the therapeutic effect of acupoint selection by heavenly stems on eosinophils(EOS) in guinea pigs with asthma.Methods Forty-eight guinea pigs were randomized into four groups: group A(timely electroacupuncture at the period of the day from 3 p.m.to 5 p.m.),group B(un-timely electroacupuncture at the period of the day from 9 a.m.to 11 a.m.),group C(model group) and group D(normal control group).Asthma was induced by chicken ovalbumin in groups A,B and C.The hematoxylin-eosin(HE) staining method and terminal-deoxynucleotidyl transferase-mediated nick end labeling(TUNEL) method were applied in the pathological and quantitative observation of eosinophils(EOS) in lung slices.Results The number of EOS was increased in model group(P

Objective To investigate the effects of rapamycin on renal tissue and function of rats with protein overload nephropathy and to explore the protective mechanism of losartan. Methods Experimental rat models with protein overload nephropathy, induced by intraperiotoneal injection of BSA (2. 0 g/d)into female Wistar rats, were divided into three groups: control group, rapamycin group(injected intraperitoneally with rapamycin) and losartan group(injected intraperitoneally with rapamycin and given orally with losartan). At different time points, the quantity of 24-hour urine protein and renal function were measured, and the morphologic changes of renal tissues were evaluated by HE staining and electron microscope. Results Both at day 7 and day 14, rats received BSA developed intense proteinuria. At day 7, compared with control group, 24-hour proteinuria increased markedly in rapamyein group (P<0.05). Nevertheless,proteinufia was notably alleviated in losartan group (P<0.05). At day 14, 24-hour-urine protein of rapamycin group was also significantly higher than that of the losartan group (P<0.05), but therewas no significant difference between control group and losartan group (P>0.05). Proteinuria and intratubular albumin cast formation were alleviated notably in losartan group. The fusion of focal podocytes in rapamycin group was obvious in comparison with control group. Conclusions Rapamycin can agrravate proteinuria in rats with protein overload nephropathy through changing the barrier of glomerular filtration by damaging podocytes. Furthermore, losartan can alleviate severe proteinuria induced by rapamycin.

AIM: To investigate the apoptosis in primary gastric cancer cells induced by resveratrol, and the relation between this apoptosis and expression of bcl- 2 and bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell gowth inhibitory rate. Transmission electron microscopy and TUNEL staining were used to quantitatively and qualitively detect the apoptosis of primary gastric cancer cells before and after the resveratrol treatment. Immunohistochemical staining and RT - PCR was used to detect the expression of apoptosis - regulated gene bcl - 2 and bax. RESULTS: Resveratrol inhibited the growth of primary gastric cancer cells in a dose - and time - dependent manner. Resveratrol induced primary gastric cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the apoptotic indexs were 4.93% ± 0.19%, 16.74% ± 0.43%, 27.88% ±0.36%, 36.84% ± 1.07 % respectively. Immunohistochemical staining showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the positive rates of Bcl - 2 proteins were 20.68% ± 0.49%, 10.84% ±0.33%, 6.80% ± 0.34%, 3.91% ± 0.15% and the positive rates of Bax proteins were 19.79% ± 0.98%, 30.74% ±0.85%, 40.14% ± 1.17%, 60.08% ± 1.64%. After exposed to resveratrol for 24 h, 48 h, 72 h and 96 h, the density of bcl- 2 mRNA decreased progressively with elongation of time and the density of bax mRNA increased progressively with elongation of time by RT- PCR. CONCLUSION: Resveratrol is able to induce the apoptosis in primary gastric cancer. This apoptosis may be mediated by down- regulation of Bcl- 2 and up- regulation of Bax.

the effect of maintaining integrity of epithelial cells by increasing occludin protein expression,and the effect is related with the up-regulation of occludin mRNA.

Object To investigate and compare the scavenging effect of Cordyceps growing in different environment on hydroxyl radical. Methods 10-Phennanthroline-Fe 2+ oxidative assay was used to observe the effect of scavenging hydroxyl radical from H_2O_2/Fe 2+ system by the aquenous extracts of Cordyceps. Results Cordyceps from Naqu of Xizang,northwest Yunnan,and northwest Sichuan all showed us the significant effect on scavenging of hydroxyl radical produced from Fenton Reaction. The biggest values of MDR among Cordyceps specimens from various microecological environment surpassed that among specimens of Naqu of Xizang,northwest Yunnan,and northwest of Sichuan. Conclusion The aquenous extracts of Cordyceps could scavenge the hydroxyl radical from Fenton Reaction. The microecological environment for Cordyceps growing has an significant effect on its scavenging action.

Objective To investigate the change of local vaginal microenvironment in atrophic vaginitis after estrogen therapy.Methods A prospective study was conducted in 128 patients with atrophic vaginitis.The patients were treated with estriol cream by vaginal administration,and the efficacy was observed after treatment.Results The PH value was decreased and the detection rate of lactobacilli was significantly increased after estrogen therapy(P＜0.01).Average vaginal health score was also increased (P＜0.01).Interleukin-6 (IL 6) level in vaginal secretion was decreased after the treatment (P＜0.01).Interferon-γ (INF-γ) level was increased obviously after treatment,while there was no significant difference between the two groups(P＞0.05).Conclusions Estrogen can significantly improve the local vaginal microenvironment in atrophic vaginitis.Estrogen may regulate the immune function of vagina by reversing the imbalance of Th1/Th2.