Objective To investigate the regulatory effects of Zishen Yutai Pills on the expression levels of homeboxA10 (HOXA10) and its downstream target gene empty spiracles homebox 2 (EMX2) in the endometria of ovulation-inducing mice at different implantation stages. Methods Seventy-five estrous female Kunming mice were randomly divided into 5 groups, namely normal group, model group 1, model group 2, treatment group 1, treatment group 2, 15 mice in each group. The model group 1 was given short-term protocol for ovulation induction; the model group 2 was given long-term protocol for ovulation induction; the treatment group 1 was given Zishen Yutai pills (at the dose of 0.4 g/mL) on the basis of the protocol for the model group 1; the treatment group 2 was given Zishen Yutai Pills (at the dose of 0.4 g/mL) on the basis of the protocol for the model group 2; the normal group was given intragastric administration or intraperitoneal injection of the same volume of normal saline. The mRNA and protein expression levels of HOXA10 and EMX2 in mouse uterus were detected by real-time fluorescent quantitative polymerase chain reaction (qPCR) and Western blot method, respectively. Results Compared with the normal group, the mRNA and protein expression levels of HOXA10 were decreased, and the mRNA and protein expression levels of EMX2 were increased in model group 1 and model group 2(P< 0.01). Compared with the corresponding model group 1 and 2, the mRNA and protein expression levels of HOXA10 were significantly up-regulated (P < 0.01) , and the mRNA and protein expression levels of EMX2 were decreased in the treatment group 1 and 2 (P < 0.01), respectively. Conclusion Zishen Yutai Pills may improve mouse endometrial receptivity by up-regulating HOXA10 expression and inhibiting EMX2 expression.

OBJECTIVETo explore the clinical effect of the sacrococcygeal space injection for the treatment of failed back surgery syndrome.

METHODSFrom July 1998 to October 2012,47 patients with failed back surgery syndrome were treated and included 39 males and 8 females with an average age of 61.5 years old ranging from 35 to 89 years old. Among them,41 patients experienced one time of operation, 6 patients with twice of operation. Forty-one patients underwent single,bilateral fenestration or central laminectomy decompression, discectomy. Six patients underwent total laminectomy discectomy and inter body fusion and pedicle screw fixation. All patients were examined by X-ray plain film, CT or MRI before treatment. The anticoagulation was discontinuation before treatment. The needle was put into the sacrococcygeal gap at prone position in the sense of frustration,suction without cerebrospinal fluid and blood,with injection of Mailuoning (Chinese characters: see text) 15 ml. The pain was assessed by VAS before and after treatment. The Oswestry low back pain disability index and survival quality interference degree were evaluated.

RESULTSAt 1 month after treatment,the pain VAS decreased from 59.24 +/- 17.35 before treatment to 19.19 +/- 11.19 after treatment (P < 0.05); The Oswestry low back pain disability index decreased from (41.35 +/- 9.87)% before treatment to (23.17 +/- 17.56)% after treatment (P < 0.05); The survival quality interference degree decreased from 6.5 +/- 2.2 before treatment to 2.6 +/- 1.4 after treatment (P < 0.05).

CONCLUSIONThe sacrococcygeal gap injection for treatment of failed back surgery syndrome has advantages of simple, safe, fewer complications, and low treatment cost.

Adult ; Aged ; Aged, 80 and over ; Drugs, Chinese Herbal ; administration & dosage ; Failed Back Surgery Syndrome ; diagnostic imaging ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Radiography ; Sacrococcygeal Region ; diagnostic imaging

Objective To study the mediated neuroprotective effect of B-cell lymphoma/Ieukemia -2 ( Bcl -2 ) and hairy enhancer of split homologues 1 ( Hes -1 ) after cerebral ischemic preconditioning.Methods The model A of cerebral ischemia was set up by the way of middle cerebral artery occlusion.The right internal carotid artery of rat was blocked transiently 10 minutes at a time, then after 3 days the model B was established by the way of model A.The sham operation group was only isolated carotid artery.The time 2, 3, 7, 14 days after the proce-dure , the neurobehavioral function in rat was evaluated by the way of Zea-Longa.The volume of cerebral infarction was measured by 2 , 3 , 5-triphenyltetrazolium chloride ( TTC) stain.The mRNA expression of Bcl-2 and Hes1 were assessed by real-time fluorescence quantitative polymerase chain reaction ( qRT -PCR ) .Results Compared with model A group, the neurologic impairment scores and cerebral infarction volume[ A:( 40.00 ±4.47 )%, B:( 19.00 ±2.75 )%, respectively ] of rats in model B group had obvious discrepancy( P<0.05).Hes1 mRNA expression in model A and model B were higher than sham operation group, model B were higher than model A, the difference had significantly (P<0.05).Bcl-2 mRNA expression, model B were significantly higher than sham group and model A at 2 days, the difference had significantly( P<0.05).Conclusion The Bcl-2 and Hes1 mRNA ex-pression increased after cerebral ischemia precondition, may be associated with nerve protective effect.

Objective To investigate the expression levels of CD40,sCD40L,hs-CRP,WBC in acute coronary syndrome ( ACS)patients and the association between CD40-1C/T single nucleotide polymorphism and risk of ACS in Han Chinese,moreover,the regulatory effects of IFN-γ and fluvastatin on the expression of CD40 in peripheral blood mononuclear cell(PBMNC)were also observed.Methods (1) 160 ACS patients and 92 control patients diagnosed by coronary angiography were recruited.Enzyme linked immunosorbent assay,particle enhanced immunoturbidimetric assay,flow cytometry were used to detect the levels of soluble CD40L,hs-CRP,and WBC count.(2)The CD40 genotype and allele frequency were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and DNA sequencing technology.(3)PBMNC were separated by density gradient centrifugating heparinized venous blood from 40 ACS patients,cultured for 24 h with or without 100 ng/ml IFN-γ in the absence or present of 10 μmol/L fluvastatin.The CD40 expression levels were then detected by flow cytometry. Results Inflammatory cytokine CD40、sCD40L、hs-CRP levels were significantly higher in ACS patients than in controls.The CD40-1C allele frequency was 0.606 in ACS group and 0.489 in controls,while T allele frequency was 0.394 in ACS group and 0.511 in controls.The frequency of CC genotype was significantly higher in ACS group than in controls(P＜0.01).C allele carriers had significantly higher risk of ACS (OR=1.608,95%CI:1.12-2.32,P=0.011).CD40 production increased after 24 h culturing and the CD40 levels were significantly higher in subiects with CC genotype than that in subiects with CT or TF genotype[CC:(14.78 ±4.56)MFI,CT:(11.98±4.12)MFI,TT:(9.86±3.83)MFI,P＜0.05],IFN-γ further increased PBMNC CD40 expressions in all subjects after culturing for 24 h and fluvastatin equally inhibited IFN-γ induced PBMNC CD40 expression from various genotypes(CC,CT,TT was 30.3%,26.3%,29.3% respectively,all P＞0.05).Conclusion Inflammatory cytokines were increased in ACS patients and CD40-1C/T polymorphism iS associated with higher risk for ACS in Han Chinese.

Objective To assess the dietary risk of organophosphates pesticides residues in some vegetables in Shaanxi Province. Methods The monitoring data of organophosphorus pesticides in vegetables from 2012 to 2016 were derived from Shaanxi Province Food Contamination Monitoring Network. The exposure of organophosphorus to vegetables by using exposure risk index (ERI) , dietary exposure risk index (RI) and dietary exposure risk assessment. Results Exposed risk index (ERI) of organophosphorus in some vegetables in Shaanxi Province ranged from 1.25E-06 to 1.87E-01, and among them, the highest ERI of clozaptid in garlic was 1.87E-01, followed by onion. In some vegetables, the highest exposure to organophosphorus was isocarbophos of fresh beans with an exposure of 4.27E-02 μg / (kg body weight · day) and the lowest dietary exposure was bulbs, and stems and fresh beans were 2.00E-04μg / (kg body weight·day) . The exposure of organophosphorus to all kinds of vegetables was less than their respective daily allowable intake (ADI) . The risk of dietary exposure to organic- phosphorus in vegetables was 187％ and greater than 100％ for the risk index (RI) of lettuce, and RI for all other vegetables was <100％. Conclusion The dietary exposure and risk index of organophosphates pesticides in some vegetables in Shaanxi Province was safe, and more attention should be paid to dimethoate and isazofos.

PURPOSETo investigate the changes of cerebral hemodynamics pre- and post-ventricular drainage in patients with posttraumatic acute diffuse brain swelling.

METHODSTwenty-four cases of traumatic diffuse brain swelling were analyzed retrospectively. Patients in nonsurgical group were treated by medicine therapy. Patients in surgical group were treated by external ventricular drainage plus medicine therapy. The first CT perfusion scan was completed within 4-5 h after trauma and scanned again after 7 days. The changes of perfusion parameters in area-of-interest in two groups were analyzed and compared before and after treatment.

RESULTSCompared with the nonsurgical group, the value of cerebral blood volume, cerebral blood flow and mean transit time in bilateral frontal temporoparietal grey matter, basal ganglia, cerebellum, and brain stem at pre- and post-therapy were increased significantly (p < 0.05) in surgical group, and consequently the prognosis of patients undergoing surgery was also better than that of nonsurgical group.

CONCLUSIONExternal ventricular drainage can improve cerebral perfusion and increase survival quality for the patients with posttraumatic acute diffuse brain swelling.

Adult ; Aged ; Brain ; physiopathology ; Brain Edema ; physiopathology ; therapy ; Brain Injuries ; complications ; Cerebrovascular Circulation ; Drainage ; Female ; Hemodynamics ; Humans ; Male ; Middle Aged ; Retrospective Studies

Objective :To explore correlation among serum levels of high sensitive C reactive protein (hsCRP) ,homocys-teine (Hcy) and severity of ischemic stroke in patients with ischemic stroke .Methods :A total of 131 patients with ischemic stroke were enrolled as stroke group .According to neurologic impairment score ,they were divided into mild group (n＝59) ,medium group (n＝41) and severe group (n＝31) ;according to infarct size ,they were divided into lacunar infarction group (n＝55) ,small infarction group (n＝46) and large infarction group (n＝30).Another 98 healthy subjects undergoing physical examination during the same period were selected as healthy control group .Serum levels of hsCRP and Hcy were measured in all groups ,and their correlation with clinic neurologic impairment degree and infarct size in patients with ische-mic stroke were analyzed .Results :Compared with healthy control group ,there were significant rise in serum levels of hsCRP and Hcy in stroke group ,P＝0.001 both .Compared with mild group ,there were significant rise in serum levels of hsCRP [ (4.43 ± 0.42) mg/L vs.(6.78 ± 1.48) mg/L vs.(9.52 ± 1.73) mg/L] and Hcy [ (17.49 ± 1.32) μmol/L vs. (22.18 ± 1.83) μmol/L vs.(26.01 ± 2.37) μmol/L] in medium group and severe group ,and those of severe group were significantly higher than those of medium group , P＝0.001 all ;compared with lacunar infarction group ,there were signif-icant rise in serum levels of hsCRP [ (4.13 ± 0.53) mg/L vs.(7.61 ± 1.47) mg/L vs.(9.49 ± 2.18 ) mg/L] and Hcy [ (18.49 ± 3.27) μmol/L vs.(22.53 ± 4.28) μmol/L vs.(27.38 ± 5.12) μmol/L] in small infarction group and large in-farction group ,and those of large infarction group were significantly higher than those of small infarction group (P＝0.001 all).Pearson correlation analysis indicated that serum serum levels of hsCRP and Hcy were significant positively correlated with clinic neurologic impairment score and infarct size in patients with ischemic stroke (r＝0.253～0.641 ,P＜0.01 all). Conclusion :Serum levels of hsCRP and Hcy are important indexes assessing severity of ischemic stroke .

Objective:To analyze the difference of life expectancy and healthy life expectancy among Shanghai residents of different gender and age groups.Methods:Compare the trends of life expectancy among Shanghai and other longevity countries/regions. With the disability weights of GBD, Sullivan method was applied to calculate the healthy life expectancy in Shanghai and analyze the loss of healthy life years among the population of different age groups and genders.Results:In the past 40 years, life expectancy had increased by 10.86 years in Shanghai. In 2016, the life expectancy of Shanghai residents was 83.18 years old, and 80.83 years old for males and 85.61 years old for females. The healthy life expectancy of Shanghai residents was 69.46 years, and 68.68 years for males and 70.23 years old for females. The gap with life expectancy was 13.72 years old, 12.15 years old and 15.38 years old, respectively. They account for 16.49%, 15.02% and 17.97% of life expectancy, respectively. The healthy life expectancy of women in all age groups is higher than that of men with the average gap of 1.76 years. The difference between the two is as small as 1.36 years at 20-24 years old, and as large as 2.24 years at 70-74 years old. The loss rate of healthy life expectancy increases with age, with women higher than men before age 65 and vice versa after age 65 years old.Conclusions:The life expectancy in Shanghai has reached the world leading level, but the healthy life loss is still large. It is necessary to further improve the life quality with the reducing mortality rate, especially for women and men over 65 years old.

Accurate identification of compound-protein interactions (CPIs) in silico may deepen our understanding of the underlying mechanisms of drug action and thus remarkably facilitate drug discovery and development. Conventional similarity-or docking-based computational methods for predicting CPIs rarely exploit latent features from currently available large-scale unlabeled com-pound and protein data and often limit their usage to relatively small-scale datasets. In the present study, we propose DeepCPI, a novel general and scalable computational framework that combines effective feature embedding (a technique of representation learning) with powerful deep learning methods to accurately predict CPIs at a large scale. DeepCPI automatically learns the implicit yet expressive low-dimensional features of compounds and proteins from a massive amount of unla-beled data. Evaluations of the measured CPIs in large-scale databases, such as ChEMBL and Bind-ingDB, as well as of the known drug-target interactions from DrugBank, demonstrated the superior predictive performance of DeepCPI. Furthermore, several interactions among small-molecule compounds and three G protein-coupled receptor targets (glucagon-like peptide-1 recep-tor, glucagon receptor, and vasoactive intestinal peptide receptor) predicted using DeepCPI were experimentally validated. The present study suggests that DeepCPI is a useful and powerful tool for drug discovery and repositioning. The source code of DeepCPI can be downloaded from https://github.com/FangpingWan/DeepCPI.

Objective:To explore the clinical effect of the sacrococcygeal space injection for the treatment of failed back surgery syndrome. Methods:From July 1998 to October 2012,47 patients with failed back surgery syndrome were treated and included 39 males and 8 females with an average age of 61.5 years old ranging from 35 to 89 years old. Among them ,41 pa-tients experienced one time of operation,6 patients with twice of operation. Forty one patients underwent single,bilateral fen-estration or central laminectomy decompression ,discectomy. Six patients underwent total laminectomy discectomy and inter body fusion and pedicle screw fixation. All patients were examined by X ray plain film,CT or MRI before treatment. The anti-coagulation was discontinuation before treatment. The needle was put into the sacrococcygeal gap at prone position in the sense of frustration,suction without cerebrospinal fluid and blood,with injection of Mailuoning (脉络宁) 15 ml. The pain was as-sessed by VAS before and after treatment. The Oswestry low back pain disability index and survival quality interference degree were evaluated. Results:At 1 month after treatment,the pain VAS decreased from 59.24 ±17.35 before treatment to 19.19 ± 11.19 after treatment(P<0.05);The Oswestry low back pain disability index decreased from (41.35±9.87)%before treatment to (23.17±17.56)%after treatment (P<0.05);The survival quality interference degree decreased from 6.5±2.2 before treat-ment to 2.6±1.4 after treatment (P<0.05). Conclusion:The sacrococcygeal gap injection for treatment of failed back surgery syndrome has advantages of simple,safe,fewer complications,and low treatment cost.