Objective To investigate the prevalence and awareness status of hypertension in adult Gansu Gannan Tibetan population.Methods A total of 6948 adult ( aged 18 and over) Tibetan residents were selected by random cluster multistage method.Participants were divided into city,rural dwellers and rural nomads groups.Questionnaires were collected and blood pressure measured from all participants.Results Prevalence of hypertension in this district was 24.6％ ( 1708/6948),of which city,rural dwellers and nomadic areas were 25.3％ ( 848/3340 ),22.7％ ( 628/2765 ),27.5％ ( 232/843 ),respectively.Prevalence of hypertension was significantly higher in city than in rural dwellers group ( P ＜0.05 ),significantly higher in rural nomads group than in city and rural dwellers groups ( all P ＜ 0.05 ).Awareness,treatment and control rates of hypertension in city group were 35.7％ (303/848),25.1％ (213/848),6.9％ (59/848),respectively:in rural dwellers group were 28.3％ (178/628),19.3％ ( 121/628),4.7％ ( 30/628 ),respectively:in rural nomads group were 16.8％ ( 39/232 ),8.6％ ( 20/232 ),2.2％(5232),respectively.Rates of awareness,treatment and control were significantly lower in rural dwellers than in city group ( all P ＜ 0.01 ),and significantly lower in rural nomads group than in city and rural dwellers groups ( all P ＜ 0.01 ).Conclusions Prevalence of hypertension in Gansu Gannan Tibetan population aged 18 and over is high.Awareness,treatment and control rates of hypertension are low,especially in rural dwellers and rural nomads residents.

Based on the outpatient interview and literature review,the initial framework of the outpatient experience of human caring scale was formed with 9 dimensions of outpatient process.The research aim was to improve the scale by Delphi method.Sixteen experts in medical management,human caring or medical education were invited to evaluate the importance of the dimensions and items of the scale and provided some expertise via filling out the Delphi consultation questionnaires twice in the consulting round.In the first round,the recovery rate showing the experts' positivity was 80％;the coefficient of reliability (Cr) ascertaining the authority of the evaluation was 0.92;the mean and full mark ratios responding the concentration of the evaluation were 2.88-4.94 and 6.25％-93.75％ respectively;the coefficients of variation (CV) and the Kendall's W determining the concordance of the evaluation were 5.06％-52.15％ and 0.21-0.24 respectively.In the second round,the recovery rate was 93.75％;the Cr was 0.93;the mean was 3.93-4.93;the full mark ratios were 26.67％-93.33％;the Kendall's W was 0.14-0.31,the CV was 5.25％-23.61％.Via the two-round Delphi study,the scale that included 10 dimensions and 61 items has been improved.Ten dimensions are pre-hospital medical service,guidance,registration,waiting,diagnosis & treatment,paying,inspection & assay,medicine receiving,therapy/injection/transfusion and global evaluation.It was concluded that Chinese scholars have paid high attention to human caring and outpatient experience.The experts have given high agreements about the dimensions which were established with Chinese outpatient process.The dimensions are different from the similar researches about outpatient experience study.In the future,it is necessary to survey the outpatients to test the construct validity,internal consistency reliability and others of the scale to improve the scale.

OBJECTIVETo explore the damage of respiratory system in the traffic policemen induced by automobile tyre colloidal particle and its allergenicity.

METHODSThe respiratory system symptoms in 445 traffic policemen working outside their offices and 243 controls were investigated and their pulmonary ventilation function index such as FVC, FEV(1.0), MMF and V(50) were determined. The specific IgE antibody of automobile tyre colloidal particle of their serum was determined and the skin-prick test of automobile tyre colloidal particle antigen was performed. Sixty-six traffic policemen working outside their offices and 5 controls with the positive of IgE antibody among them were detected by nasal mucosa provocation test. Sixty-six traffic policemen working outside their offices with the positive of IgE antibody were determined by Terbutaline inhalation test.

RESULTSThe positive rate of respiratory system symptoms of traffic policemen such as cough, stethocatharsis, short breath, nasal obstruction, sneeze and nose running was 38.02%, 27.03%, 20.00%, 23.08%, 27.47%, 32.09% and 34.95% respectively and significantly higher than those of the control with significant difference (P < 0.01) or (P < 0.05). The positive rate of specific IgE antibody of automobile tyre colloidal particle, skin- prick test and nasal mucosa provocation test was 14.51%, 23.73% and 54.55% respectively with significant difference (P < 0.01) and (P < 0.05). The percentage, the actual figure compared with the prediction figure, of the index of pulmonary ventilation function (FVC, FEV(1.0) MMF and V(50)) of traffic policemen were significantly lower than those of the control. Terbutaline inhalation test in 66 positive subjects of specific IgE antibody of automobile tyre colloidal particle was positive in 44 subjects, accounting for 9.67% in all policemen investigated.

CONCLUSIONThe automobile tyre colloidal particle is one of etiological factors that induce pulmonary ventilation function damage and could result in allergic asthma of traffic police.

Adult ; Allergens ; analysis ; Automobiles ; Humans ; Immunoglobulin E ; blood ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Police ; Respiratory System ; drug effects ; Rubber ; adverse effects ; Young Adult

Objective To evaluate the effect of intravenous thrombolysis with urokinasc for treatment of acute cerebral infarction due to vertebrobasilar artery ischemia.Methods Thirteen pafients with a clinical diagnosis of acute cerebral infarction arising from vertebrobasilar artery ischemia received thmmbolytic therapy with urokinase(5×105-1.5×106U) infused intravenously within 1 h.The NIH Stroke Scale(NIHSS)was used to evaluate the patients'neurological function before and at 0,6,24 hand at 7 and 14 days after the treatment.Modified Rankin Scale(MRS)was used for neurologiical assessment 3 months after the treatments. Results The mean NIHSS score of the patients was 17.46±6.19 before treatment and rapidly decreased to 7.92±6.43 24 h after the thrombolytic therapy.Three months after the therapy,8 patients had favorable clinical outcomes defined as capability of independent living(MRS score of 0-2);2 patients had a poor outcomes with assisted living,and 3 patients died.Bleeding occurred in 4 cases including 2 cerebral hemorrhage cases. Conclusion Favorable clinical outcome can be achieved with intravenous urokinase therapy in the majority of patients with acute cerebral infarction due to vertebrobasilar ischcrnia.

Objective To investigate the need for health knowledge of hemodialysis patients of different dialysis ages,in order to provide basis for health education.Methods Self-designed questionnaire was used to look into the need for health knowledge of 100 maintenance hemodialysis patients of different dialysis ages.Results Patients of different dialysis ages had different acknowledge of the understanding of health education,the necessity of health education,the influence of health education on life quality.The differences were statistically significant ( F =28.149,18.739,24.407,respectively ; P ＜ 0.001).Patients who had received dialysis less than 1year paid more attention to the effect of hemodialysis,the maintenance of vascular access and instantaneous complications.Patients who had received dialysis more than 1year but less than 5 years laid emphasis on the sufficiency of hemodialysis,drug use during hemodialysis,examination index and long-dated complications.Patients who had received dialysis more than 5 years wanted more knowledge on the long-dated complications of hemodialysis,new developments of dialysis treatment and life quality improvement,etc.Patients of different cultural levels had different requirements for health education modes.Explanation from doctors and nurses,videos were first choice of most patients,but education modes such as experience introduction from wardmate,reading related books,publicity column,lectures and internet search were differently welcomed between patients of different cultural levels,and the differences were statistically significant (F=14.950,6.020,11.772,6.744,13.057,respectively;P＜0.05).Conclusions As the main enforcers of health education,paramedics need to further popularize the concept of health education,and pay attention to the diversity of education mode according to the needs of patients of different dialysis ages,in order to fulfill the health education work.

Objective To investigate the impacts of LINC00943 on proliferation,apoptosis,and invasion of esophageal squamous cell carcinoma(ESCC)cells by regulating the miR-126-5p/HOXB2 axis.Methods QRT-PCR was applied to detect the expression of LINC00943 in 45 ESCC tissues and cell lines;The effects of LINC00943 knockdown on the proliferation,apoptosis and invasion of KYSE30 cells were detected by MTT assay,flow cytometry and Transwell chamber.Western blot was applied to detect the expression of proliferating cell nuclear antigen(PCNA),Bcl-2 associated X protein(Bax),anti apoptotic factor B cell lymphomatoma-2(Bcl-2),cleaved caspase-3,matrix metalloproteinase-2(MMP-2),MMP-9,and HOXB2;dual luciferase reporter gene experiment was applied to verify the relationship between miR-126-5p and LINC00943 and HOXB2;the ESCC nude mouse in vivo model was constructed and separated into si-NC and si-LINC00943 groups,the tumor mass and volume were measured,qRT-PCR was applied to detect the expression of miR-126-5p in transplanted tumor tissue,immunohistochemistry was applied to detect the expression of HOXB2 protein in transplanted tumor tissue,and TUNEL staining was applied to detect cell apoptosis in tumor tissue.Results The expression of LINC00943 mRNA increased in ESCC tissues and cell lines(P＜0.05);Compared with the si-NC group,the proliferation activity,migration and invasion cell number,HOXB2,PCNA,MMP-2,MMP-9 and Bcl-2 protein expression of KYSE30 cells in the si-LINC00943 group were decreased,and the expression of miR-126-5 p,Bax,Cleaved Caspase-3 and apoptosis rate were increased(P＜0.05);downregulation of miR-126-5p was able to weaken the inhibitory effect of LINC00943 knockdown on the malignant behavior of KYSE30 cells(P＜0.05);dual luciferase reporter gene experiment confirmed the targeted regulatory relationship between miR-126-5p and LINC00943,and miR-126-5p and HOXB2(P＜0.05);The ESCC nude mouse model was constructed in vivo and divided into si-NC and si-LINC00943 groups.The tumor mass,tumor volume,expression of miR-126-5p and HOXB2 in transplanted tumor tissues and apoptosis rate of tumor cells were measured.(P＜0.05).Conclusion LINC00943 is highly expressed in ESCC tissues and cell lines.Knocking down LINC00943 may inhibit the expression of HOXB2 by up-regulating the expression of miR-126-5p,promote the apoptosis of ESCC cells,and inhibit their proliferation,migration and invasion.

Objective To investigate the application effect of hierarchical health education on hemodialysis patients.Methods 6 months of hierarchical health education were conducted on 100 cases of maintenance hemodialysis patients.Self-designed questionnaire was used to investigate patients' health knowledge before and after education,survival of kidney disease scale (KDQOL-SFTM) was used to study patients' quality of life before and after education,and patients' satisfaction questionnaire drawn up by nursing department was used to compare patients' satisfaction before and after education.Results Patients who had dialysis for less than 1 year,1-5 years,more than 5 years,and whose health knowledge reached the standard were respectively 19,38 and 19 after hierarchical health education,all of which were higher than before (7,17 and 11),and the differences were statistically significant (x2 =9.968,15.590,5.333,respectively; P ＜ 0.05).All items in KDQOL-SFTM got more points after hierarchical health education than before,the difference was statistically significant (P ＜0.01).Patients' satisfaction was 92％ after hierarchical health education and 78％ before,the difference was statistically significant (x2 =7.686,P ＜ 0.01).Conclusions Hierarchical health education on patients with different dialysis years is effective to enhance patients' health knowledge,harmonize nurse-patient relationship and improve patients' quality of life.

AIMTo examine the impact and prognostic significance of alpha-tocopherol associated protein (TAP) expression in a series of prostate cancer patients.

METHODSTissues from 87 patients underwent radical prostatectomy were examined for TAP expression by immunohistochemistry. The relationships of the staining results, the clinic pathological characteristics and the recurrence times were analyzed.

RESULTSCompared with the adjacent areas of normal and benign glands, immunoreactivity of TAP was reduced in areas of prostate cancer. A lower TAP-positive cell number per mm(2) of the largest cancer area (defined as TAP-PN) was associated with higher clinical stage (r = -0.248, P = 0.0322). Inverse associations were found among the TAP-PN and positive lymph nodes (r = -0.231, P = 0.0325), preoperative prostate-specific antigen (PSA) levels (r = -0.423, P = 0.0043), tumor size (r= -0.315, P= 0.0210) and elevated tumor cell proliferation, which was indicated by the staining of Ki-67 (r = -0.308, P = 0.0026). TAP-PN was a significant predictor of recurrence univariately (P = 0.0006), as well as multivariately, adjusted for known markers including preoperative PSA, clinical stage, Gleason score, surgical margin, extra-prostatic extension, seminal vesicle invasion and lymph node metastasis (P = 0.0012).

CONCLUSIONReduced expression of TAP was associated with the cell proliferation status of prostate cancer, adverse pathological parameters and the increased risk of recurrence.

Aged ; Carrier Proteins ; biosynthesis ; genetics ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Humans ; Ki-67 Antigen ; biosynthesis ; Lipoproteins ; biosynthesis ; genetics ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; etiology ; Prostatic Neoplasms ; metabolism ; pathology ; Trans-Activators ; biosynthesis ; genetics

A temperature-responsive hydrophilic block copolymer fluorescence sensor based on selective analysis of Al3+was designed and synthesized. The polymer fluorescent probe (L64-BTPA-SHMA) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization and characterized by 1 H NMR,and the recognition performance of the polymer probe was studied by fluorescence spectroscopy. It was found that the fluorescence response of Al3+detected by this polymer fluorescence probe was significantly affected by temperature. The higher the temperature, the lower the fluorescence intensity was observed. The results showed that 0.1 g/L polymer fluorescent probe had a good selectivity to Al3+in buffer solution at 25℃and pH 7.4,and was not affected by other metal ions. A fluorescence detection method based on this polymer probe for Al3+was developed. In this method,the fluorescence intensity was linear with Al3+concentration in the range of 2. 0 -18. 0 μmol/L, with a correlation coefficient of 0. 9977 and a detection limit of 1.43 μmol/L. The fluorescence response between the polymer fluorescence chemosensor and Al3+could be altered by controlling the temperature change. The successful application of the polymer fluorescence probe for detecting Al3+residues in agricultural products has practical application value.

BACKGROUND & OBJECTIVE: Postoperative tissue adherence, scarring and radiotherapy often lead to extrinsic compression and stricture in the distal ureter of the patients who had history of pelvic malignancies. Our aim was to evaluate the efficacy and safety of endourologic techniques in treating this kind of ureteral obstruction. METHODS: From Jan. 1998 to Mar. 2007, 46 patients with obstruction in the distal ureter and had history of pelvic malignancies underwent endoscopic treatments at the Third Affiliated Hospital of Sun Yat-sen University for relief of the obstruction. Perioperative and follow-up data were analyzed. RESULTS: Of the 46 patients, 25 underwent laparoscopic ureterolysis and ureteroneocystostomy, 18 underwent placement of ureter stent under ureteroscope, 3 underwent percutaneous nephrostomy. No severe complication was recorded. The mean operating time was 82.5 min (range, 30-140 min). The mean blood loss was 45.5 ml (range, 5-180 ml). No blood transfusion was needed. The median follow-up time was 18.2 months (range, 3 months to 6.5 years). Three months after operation, B-ultrasonography and intravenous urography (IVU) showed that 39 (84.8%) patients had recovered normal renal function, the other 7 (15.2%) had hydronephrosis relief and renal function improvement. Nuclear renal scanning showed that the mean postoperative glomerular filtration rate (GFR) in the obstructive kidney was higher than the preoperative level (37.6 ml/min vs. 21.3 ml/min, P＜0.05). No stricture in the uretero-bladder anastomotic stoma was recorded. CONCLUSION: Endoscopic operation is an effective and feasible option for managing some selected kinds of distal ureteral obstruction caused by postoperative tissue adherence and radiotherapy in the patients with history of pelvic malignancies.