2.Effect of injecting allogeneic mesenchymal stem cells on cellular immunity in rat in vivo
Jinming YU ; Dehong CAI ; Hua ZHANG ; Xiaopeng YUAN ; Hong CHEN
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To investigate the effects of injecting allogeneic mesenchymal stem cells(MSCs) on the cellular immunity in rat in vivo.Methods Bone marrow-derived MSCs were isolated from Wistar rats.The purity of MSCs was identified by morphological examination with microphotography,and the phenotypes were identified with flow cytometry.Twenty SD rats were randomly divided into 4 groups.Different concentrations of MSCs(5?106/ml for group A,5?105/ml for group B,and 5?104/ml for group C,respectively) and PBS(for group D) were given to allogeneic SD rats via intravenous infusions.The suppressive effect of MSCs on lymphocytes proliferation in recipient rat was analyzed using mixed lymphocyte cultures(MLR) 10 days after cultivation.At the same time,proportions of CD4+,CD8+ and CD4+CD25+/CD4+ T-lymphocytes in peripheral blood and spleen were analyzed with flow cytometry.Results Proliferation rate of splenic lymphocytes in group A(5?106/ml MSCs,8.58%?0.27%) was markedly lowered compared with that in group D(PBS,24.40%?5.21%,P
3.Effect of Dexamethasone on Excitatory Amino Acid and Monoamine Neurotransmitter in Cerebral Tissue of Neonatal Rats with Hypoxia-Ischemia
bao-hong, WANG ; yu, JIN ; cai-xia, AN ; xin-hua, YE
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the value of dexamethasone(DEX) for neuronal cell injury and death by observing the effect of DEX on excitatory amino acid(EAA) and monoamine neurotransmitter in cerebral tissue of neonatal rat with hypoxia-ischemia.Methods Hypoxic-ischemic neonatal rat models were established,the levels of EAA and monoamine neurotransmitter in cerebral tissue were analyzed by using capillary electrophoresis and fluorospectrophotometry method.The rats were divided into 4 groups: small dose DEX group pre-treated with DEX(0.5 mg/kg) prior to hypoxia-ischemia,large dose DEX group pre-treated with DEX(10 mg/kg) prior to hypoxia-ischemia,HIE group and shamful operation group.Results The levels of EAA and monoamine neurotransmitter contents in HIE group were significantly higher than those in shamful operation group(P0.05).EAA contents of large dose DEX group greatly decreased compared with HIE group (P
4.Effectiveness of amniotic membrane transplantation combined with mitomycin C in the treatment of pterygium: a meta-analysis
Yin-Wei, SONG ; Ai-Hua, YU ; Xiao-Jun, CAI
International Eye Science 2010;10(12):2238-2241
·AIM: To evaluate the recurrence rate and safety of amniotic membrane transplantation (AMT) augmented with mitomycin C (MMC) compared with amniotic membrane transplantation alone during the pterygium excision.·METHODS: We took a meta-analysis on this program.Pertinentstudieswereselectedthroughextensive searches of the Cochrane Library, MEDLINE, EMBASE,CBMdisc, CNKI. Pooled estimates were carried out in RevMan software V4.2.·RESULTS: Six trials reported postoperative recurrence rate of pterygium, included 882 eyes, three trials reported the complications. The results of meta-analysis showed that recurrence rate of AMT plus MMC group was 5.41%,AMT alone group was 16.89%, relative risk (RR) was 0.32, 95%CI ranged from 0.19 to 0.56, Zwas 4.06, P< 0.001. Two trials reported early complication as punctata keratitis, the incidence rate of AMT plus MMC group and AMT alone group were 17.14% and 0.00%, RR was 12.11,95%CI ranged from 1.62 to 90.76.·CONCLUSION: Amniotic membrane transplantation with MMC is associated with lower recurrence rate compared with amniotic membrane transplantation alone in pterygium excision,whether accompanied a higher risk with adverse events need more investigation.
7.Current Situation of TCM Human Resources Distribution and Equity Research in China
Hua-Yu WU ; Cai-Yun GAO ; Yu LIU ; Guo-Wu DING
Chinese Journal of Information on Traditional Chinese Medicine 2018;25(3):7-11
Objective To discuss existing problems in talent team construction; To provide evidence for the government to enact proper strategies based on the analysis of the current situation of TCM practitioners (assistants) distribution in China from quantity, quality and distribution equity. Methods The research is studied through conducting a descriptive analysis of TCM practitioners (assistants) from quantity, education, job titles, ages, and other basic conditions and changes. Gini coefficient was used to evaluate the distribution of TCM practitioners (assistants). Results The quantity of TCM practitioners (assistants) increased by 53.7% from 2010 to 2015. Concerning the quality of personnel, the ratio of the personnel with bachelors degree or above increased from 38.6% to 44.2%, and a slight increase was observed with the personnel with senior title. In terms of age structure, the proportion of the age of TCM practitioners (assistants) under 25 years old, 35 to 59 years old decreased, and the proportion of the age of TCM practitioners (assistants) above 60 years old, 25 to 34 years old increased. From the perspective of equity, there is a difference between the provincial-level administration of human resources distribution. The Gini coefficient as per population distribution was around 0.1, which indicated good equity. From the geographical area configuration, the Gini coefficient was approximately 0.58, which showed high level of disparity. Conclusion There are problems of insufficient quantity, low quality and distribution disparity among regions in TCM practitioners (assistant) in China.
8.Breviscapine listed on progress of new varieties and dosage form research.
Li-Hua TIAN ; Li-Zhong ZHAO ; Jia GU ; Jun CAI ; Lei YU
China Journal of Chinese Materia Medica 2014;39(19):3719-3722
Breviscapine, a flavone glucuronide, is a cardiovascular medicine extracted from a Chinese herb Erigeron breviscapinus. Modern pharmacological research shows breviscapine has a therapeutic effect on cardiovascular diseases such as cerebral thrombus,cerebral ischemia hemiparesis and platelet aggregation et al. However, its poor water solubility and low bioavailability in vivo severely restrict the clinical application. In this article, we reviewed the marketing preparations of breviscapine and its research progress on the new dosage forms.
Biomedical Research
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Brain Ischemia
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drug therapy
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal
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administration & dosage
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economics
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Flavonoids
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administration & dosage
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economics
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Humans
9.The pathological findings and inflammatory responses in the lung tissue of neonatal rats following lipopolysaccharide treatment.
Xu-xu CAI ; Yue DU ; Xiao-hua HAN ; Yun-xiao SHANG ; Yu-kun HAN
Chinese Journal of Pediatrics 2003;41(8):617-618
Animals
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Animals, Newborn
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Bronchoalveolar Lavage Fluid
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chemistry
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cytology
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Female
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Lipopolysaccharides
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toxicity
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Lung
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drug effects
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enzymology
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pathology
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Male
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Neutrophils
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cytology
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Peroxidase
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analysis
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Rats
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Rats, Wistar
10.The study of expression of matrix metaUoproteinases-2 in patients with colorectal cancer
Ya MA ; Guang-Ling CAI ; Guang-Yin YU ; Wei-Hua YIN ; Li CHEN ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective Research the correlation between expression of MMP-2 in colorectal cancer and patho- logical factors,or biological behaviors in colorectal cancer.Methods The expression of MMP-2 in 68 cases with col- orectal cancer were detected by immunohistochemical staining.Results The expression of MMP-2 in colorectal can- cer was not correlated with tumor region and histologic type,but related with depth of tumor invasion and metasta- sis.The frequency of positive cases in patients with colorectal cancer with of lymph,node metastasis was significantly higher than that in patients without lymph node metastasis(P