3.Research Progress on Bacillus thuringiensis Chitinase
Wei LU ; Jun CAI ; Yue-Hua CHEN ;
Microbiology 1992;0(01):-
The leading bioregional pesticide,Bacillus thuringiensis,is accepted by the public and widely used biopesticide in the world.B. thuringiensis chitinase may contribute to the biocontrol of phytopathogenic fungi and enhance insecticidal activity.It helps to take full advan- tage of Bt and upgrade the efficiency.This paper reviews the progresses of B.thuringiensis chitinase.
4.Changes of intercelluar adhesion molecule-1 and inhibitor of nuclear factor ?B after lipopolysaccharide induced pulmonary hemorrhage in neonatal rats
Xuxu CAI ; Yue DU ; Yunxiao SHANG ; ETAL
Chinese Journal of Perinatal Medicine 2003;0(05):-
Objective To investigate the changes and effects of intercelluar adhesion molecule-1 (ICAM-1) and inhibitor protein of nuclear factor-?B(I?B?) in pulmonary hemorrhage induced by endotoxin lipopolysaccharide (LPS) in neonatal rats. Methods Sixty-eight seven to ten-day-old Wistar rats were separated into 2 groups randomly. In the experimental groups, LPS was injected intraperitoneally at the dosage of 5 mg/kg and the normal saline (NS) control group was injected with equal amount of NS intraperitoneally. The rats were sacrificed at 30 min, 1, 2, 4, 8, 16 and 24 h post injection. The histology of lung tissues were examined by microscope and electron microscope. The expression of ICAM-1, I?B?protein and mRNA expression were observed using Western blot and in situ hybridization. Results One hour after LPS administration, pulmonary hemorrhage and inflammatory responses were observed in the rat lungs. The ICAM-1 protein expression greatly increased at 2 h post injection and it was significantly higher than control group(178.13?8.18 vs 161.57?12.52, P
5.Effect of passive smoking during pregnancy on the expression of TNF-? and CINC in the lungs of neonatal rats
Xuxu CAI ; Yue DU ; Chunfeng LIU
Chinese Journal of Perinatal Medicine 2003;0(05):-
Objective To determine the effect of passive smoking during pregnancy on the expression of tumor necrosis factor-? (TNF-?) and cytokine-induced neutrophil chemoattractant (CINC) in the lungs of neonatal rats. Methods Sprague Dawley rats were exposed to sidestream cigarette smoke on the 1, 8 and 15 day after pregnancy for a total of 2 h each day. Those rats who were put into the box without smoke exposure were as control. The neonatal rats were delivered by cesarean section on the 20th day of gestation and birth weight was measured. Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunoadsorbent assay (ELISA) was performed to evaluate the expression of TNF-?、CINC mRNAs and TNF-? protein. Results The birth weight of neonatal rats born from those mothers who were exposed to sidestream cigarette smoke was significantly lower than that of control gruop(P
6.Analysis of the differentiated strategic objectives chosen by internal stakeholders of public hospitals in Hang-zhou
Ning CAI ; Yue YANG ; Xiaoli DAI
Chinese Journal of Hospital Administration 2010;26(2):81-84
Study of the strategic objectives chosen by hospitals from the perspective of stakeholders is now under the spotlight of researchers at large. To identify whether there exist differences in strategic objectives among various groups in the hospital and probe into the causes, the author called into play the competing values framework as the analysis tool. An evidenced-based study on public hospitals in Hangzhou identified marked differences on choices of strategic objectives among various internal stakeholders in the hospital For example, hospital administrators tend to focus on internal control, while medical doctors tend to focus on interactions between the hospital and the external environment. On this basis, further studies are proposed.
7.Change and Effect of Neurotransmitters in Spinal Cord after Spinal Cord Injury(rerview)
Yanhua CAI ; Feng YUE ; Chaoqun YE
Chinese Journal of Rehabilitation Theory and Practice 2010;16(6):540-542
The neurotransmitter plays an important role in the neurologic functional recovery of animals and patients with spinal cord injury. This article reviews the neurotransmitters in spinal cord injured of rats and the effect of some therapy on them.
8.History of World Federation of Acupuncture-Moxibustion Societies.
Hong-Cai WANG ; Liang-Yue DENG ; Bao-Yan LIU
Chinese Acupuncture & Moxibustion 2014;34(12):1235-1237
The history of the World Federation of Acupuncture-Moxibustion Societies (WFAS) was reviewed through summarizing the background and process of the establishment of WFAS. The establishment background was explained in different aspects, named the recovery of acupuncture-Moxibustion in the world, the successive setup of world acupuncture-Moxibustion organizations, the divergences of International Association of Acupuncture-Moxibustion, striding forward of China reform and opening policy as well as the attention of the World Health Organization (WHO), etc. The establishment of WFAS was introduced on the proposal from eight countries, the important time of the development of acupuncture and moxibustion in China, 1984, divergences and consensus as well as the final phase. The official establishment of WFAS represents the global benefits of acupuncture-Moxibustion colleagues. It is the international organization of acupuncture and moxibustion, contributing to the promotion of acupuncture and moxibustion in the world.
Acupuncture
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history
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organization & administration
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Acupuncture Therapy
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history
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History, 20th Century
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History, 21st Century
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Humans
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Moxibustion
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history
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Societies, Medical
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history
9.The development of World Federation of Acupuncture-Moxibustion Societies.
Hong-cai WANG ; Liang-yue DENG ; Bao-yan LIU
Chinese Acupuncture & Moxibustion 2014;34(10):1028-1030
From cooperation between World Federation of Acupuncture-Moxibustion Societies (WFAS) and World Health Organization, works of academics and standardization, acupuncture-moxibustion education, technique service, organization development, etc., the 27-year development of WFAS since 1987 was summarized and reviewed in details. The growth of WFAS witnesses and promotes the development of acupuncture and moxibustion in the world, so by learning the experience and lessons, WFAS could have a wider path, becoming a core organization for communicating and promoting the development of acupuncture, even the traditional medicine in the world.
Acupuncture
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education
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history
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organization & administration
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standards
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Acupuncture Therapy
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history
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standards
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Global Health
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history
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History, 20th Century
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History, 21st Century
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Humans
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Moxibustion
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history
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standards
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Societies
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history
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World Health Organization
10.Culture and biological characteristics of neural stem cells from caveolin-1 knockout embryonic mice
Baiyan LIU ; Yue YU ; Jian YI ; Xuemei CHEN ; Guangxian CAI
Chinese Journal of Tissue Engineering Research 2014;(23):3739-3744
BACKGROUND:Caveolin-1 is expressed in mammalian brain and involved in the normal development of the brain, which can affect the proliferation of neural stem cells in the brain. OBJECTIVE:To acquire neural stem cells from caveolin-1 knockout embryonic mice in vitro and study their biological characteristics. METHODS:The whole brain was separated from C57BL/6 mice and caveolin-1 knockout C57BL/6 mice respectively at encyesis 14-16 days. Single cellsuspension was obtained by enzyme digestion, and cultured in the conditioned medium of neural stem cells. Fol owing 7 days of primary culture, the cells were induced in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum for 7 days. RESULTS AND CONCLUSION:The major cells of the cellsuspensions from the fetal mouse brain were dead at 1 day after culture, and some single cells floated in the medium and their transmittance were better, and then they gradual y formed multicellular bal s after 3 days. A smal amount of cells were adhered at the bottom of culture plate after passage, and a great amount of cellbal s appeared after 7 days. The proliferation rate of neural stem cells from caveolin-1 knockout mice was higher than that from normal mice. The cellbal s were nestin-positive and their differentiated cells was positive for neurofilament 200, glial fibril ary acidic protein or O4, respectively. Al of the cells from normal mouse brain were positive for caveolin-1, but the cells from caveolin-1 knockout mice were negative for caveolin-1 by immunocytochemistry. Moreover, the speed of cellbal formation and the number of cellbal s in neural stem cells from caveolin-1 knockout mice were better than those from normal mice. Caveolin-1 negative neural stem cells were cultured successful y from caveolin-1 knockout mouse brain, and the results show that caveolin-1 can promote the proliferation of neural stem cells and inhibit their differentiation in vitro.