Humans ; Mining ; Noise, Occupational ; statistics & numerical data ; Occupational Exposure ; statistics & numerical data ; Work Schedule Tolerance

Objective To investigate the effect of interferon-α (IFN-α) on expression of major histocompatibility complex (MHC) class I chain-related protein A (MICA) in the malignant hematopoietic cell lines.Methods The myeloid leukemia cell lines K562 and B-cell lymphoma cell line Raji were treated with IFN-α.The expression of MICA was measured by RT-PCR and immunohistochemical staining at mRNA and protein level.The cytotoxicity of human NK cells to the IFN-α treated malignant hematopoietic cells was detected by MTT method.Results After being treated with 1000 U/ml IFN-α,up-regulation of MICA mRNA in Raji and K562 cells were respectively found in 24 h and 48 hours (t =17.016,P ＜0.05; t =5.616,P ＜0.05).After being treated with IFN-α 72 hours,up-regulation of MICA mRNA in K562 and Raji were found at the dose of 500 U/ml and 1000 U/ml dose (t =6.622,P ＜0.05; t =9.071,P ＜0.05).The mRNA and protein expression of MICA were up-regulated by IFN-o in the two malignant hematopoietic cells in a time and dosedependent manner.After being treated with 1000 U/ml IFN-o for 72 hours,the susceptibility of the two malignant hematopoietic cells to NK cytolysis was significantly increased (t =20.016,P ＜0.05; t =7.969,P ＜0.05).Moreover,the up-regulated susceptibility can be blocked by anti-MICA antibody.Conclusion IFN-α can up-regulate the MICA expression in the malignant hematopoietic cell lines and thereby enhance the susceptibility to cytolysis of NK cells.

Objective　 To study the postprandial lipid metabolism in patients with coronary heart disease.　Methods　11 CHD cases as CHD group and 15 normal as control group were selected to undergo oral fat load test. Blood samples were obtained before test and 2，4,6 and 8 hours after test. Serum TG, TC, LDL-C,HDL-C, LPO and LDL-LPO-C were calculated.　Results　1.Preprandial TG, LDL-C, LPO and LDL-LPO-C in CHD group were significantly higher than that of control group. 2. Postprandial TG, LPO, LDL-LPO-C in CHD group were significantly higher than that of control group. Postprandial HDL-C in CHD group were significantly lower than control group. 3. Postprandial TG (8h), LDL-LPO-C in CHD group were significantly higher than in the preprandial. Postprandial LDL-LPO-C in control group was significantly higher than preprandial. Conclusions 1. Postprandial high-lipemia and dyslipemia was obvious in CHD group. 2. Postprandial high-lipemia was closely related to the development of CHD. 3. Postprandial LDL-LPO-C in CHD group increased significantly and LDL-C sensitivity to oxidative intensified with postprandial time. 4. Postprandial LPO in CHD group increased significantly.

AIM:To investigate the expression and clinical significance of PAK4 in the cell lines and tissues of non-small cell lung cancer (NSCLC).METHODS:PAK4 expression in human bronchial epithelial (HBE) cells, NSCLC cell lines, NSCLC tissues and adjacent non-tumor tissues were assessed by immunohistochemistry, real-time PCR and Western blot.Prognostic value of PAK4 expression was evaluated by Kaplan-Meier analysis and Cox regression.RE-SULTS:PAK4 was over-expressed in the NSCLC cell lines at both mRNA and protein levels compared with HBE cells ( P<0.05).PAK4 was over-expressed in the NSCLC tissues at both mRNA and protein levels compared with adjacent non-tumor tissues (P<0.05).PAK4 was over-expressed in the metastatic NSCLC tissues compared with the primary NSCLC tissues (P<0.05).Higher PAK4 staining scores were positively correlated with differentiation, lymph node metastasis, distant metastasis, and clinical stage.Kaplan-Meier analysis and log-rank test showed that overall survival was significantly different between the patients with up-regulated PAK4 and the patients with down-regulated PAK4 ( P<0.05) .PAK4 over-expression was associated with NSCLC progression.CONCLUSION:Increased PAK4 expression was associated with tumor invasion, metastasis and prognosis in the patients with NSCLC.PAK4 is an important prognostic marker and potential ther-apeutic target in NSCLC.

Objective:To summarize and analyze the clinical status of tumor necrosis factor-α (TNF-α) antagonists.Methods: The clinical trials of TNF-α antagonists registered before August 2,2016 were searched on the National Institutes of Health (NIH) Clinical Trials Register,and SPSS17.0 software was used for statistical analysis of the data.Results: A total of 306 TNF clinical trials were conducted in the world,19 of them were in China.The main research diseases for bone and joint disease,autoimmune diseases,infections,blood tumors.There were 241 items in intervention study,and 56,7,55,60 items in 178 cases,accounting for 73.9% in Ⅱ,Ⅱ/Ⅲ,Ⅲ,Ⅳ,respectively.The interventional study of TNF-α antagonist clinical trials mainly focused on intervention research,The main purpose of the practical application of the process after the listing of further validation and evaluation of drug efficacy and safety.Conclusion: There are many kinds of TNF-α antagonists,and clinical indications are wide.The design of clinical trials is diversified and mature.

Weight loss may reduce the risk of diabetes,cardiovascular disease,hypertension,certain cancers and so on.Current weight-loss methods include surgery,diet and exercise.According to ingredient ratio,weight-loss diet is classified as low-fat diet,low-carbohydrate diet and high-protein diet.This review summarizes recent evidence on the efficacy of the weight-loss diet on serum lipid levels.

OBJECTIVE:To provide reference for rational use of carbapenem antibiotics in the clinic. METHODS:Enterobac-teriaceae bacteria were collected from our hospital during Jan. 2014-Dec. 2015;semiautomatic microbiological assay instrument was used for strain culture,identification and drug sensitive tests. Modified Hodge test and K-B test were adopted to confirm Klebsiella pneumoniae carbapenemases (KPC)-producing and ESBLs-producing drug resistant strains. RESULTS:During 2014-2015,1 035 strains of Enterobacteriaceae bacteria were detected in our hospital,among which there were 732 strains of Escherichia coli,157 strains of K. pneumonia,136 strains of Enterobacter cloacae and 10 strains of Serratia marcescens. Citrobacter freundii was not found. E. coli and K. pneumonia were highly sensitive to amikacin and carbapenems,but slightly sensitive to most cephalosporin. A total of 64 strains of carbapenems-resistant Enterobacteriaceae bacteria(6.18%)were detected,among which there were 31 strains of carbapenems-resistant E. coli(4.23%),30 strains of carbapenems-resistant K. pneumonia(19.11%),1 strain of carbapenems-re-sistant E. cloacae(0.74%)and 2 strains of carbapenems-resistant S. marcescens(20.00%). The samples were mainly from sputum and urine specimens,which were mainly from neonatal department and ICU. Of 64 drug resistant strains,there were 59 KPC-pro-ducing strains (92.19%) and 3 ESBLs-producing strains (4.69%). CONCLUSIONS:E. coli occupies high proportion among En-terobacteriaceae bacteria,and the number of carbapenems-resistant E. coli and carbapenems-resistant K. pneumoniae is in high lev-el. Drug resistance of Enterobacteriaceae bacteria to carbopenems may be associated with KPC and ESBLs producing. Carbapenem antibiotics should be selected rationally in accordance with medication indications and the results of drug sensitivity test.

Objective:To study the effect of thyroid function on bone metabolism. Methods:Serum FT3,FT4 were investigated by radioimmunoassay (RIA) and bone mineral density of spine (L2～4) weremeasured by dual energy x-ray absorptinmetry and other markers related to bone metabolism were alsomonitored in 30 patients with hyperthyroidism and 30 healthy volunteers. Results :The levels of FT3,FT4,ALP were significantly higher than those of the normal controls. BMD of spine decreased significantly incomparison with the controls ,and the degree of severity and incidence increased with age. Conclusion:Thy-roid hormone might speed up bone turnover directly with increased bone resorption to induce bone massloss.

AIM: To study the effect of double gene transduction mediated by lentiviral vectors on the characteristics of human embryonic stem cells (hESCs). METHODS: Using the backbone from inducibal dual and excisable transgene vector (iDuet101) , lentiviral vectors overexpressing cytotoxic T lymphocyte - associated molecule - 4 immuno-globulin (iDuet101 - CTLA4Ig) , indoleamine 2, 3 dioxygenase (iDuet101 - IDO) , and ubiquitin - C promoter - lueifer-ase - ires - puromycin ( ULIP) were constructed and packaged according to the standard recombinant techniques. Human embryonic stem cells (hESCs) were first transduced with iDuet101, iDuet101 - CTLA4Ig or iDuet101 - IDO, then, after the selection, were transduced again with ULIP. The expression and function of the exogenous genes were detected. Immu-nohistochemistry, RT - PCR and flow cytometry were applied for detection of embryoid bodies ( EB) formation in vitro and in vivo teratoma formation. RESULTS: Double - transduced hESCs showed typical shape of cell clones and positive staining of tumor rejection antigen -1 - 60 ( Tra -1 - 60 ) and octomer transcription factor - 4 ( OCT - 4 ). The formation of EB was observed, in which a - fetoprotein (AFP), paired box gene 6 ( Pax6) and Musashi 1 ( MSI1) were positively expressed. The cells formed teratomas, and the luciferase signals existed until 28 days after xeno - transplantation. CONCLUSION : Double transduction of non - transcriptional factors mediated by lentiviral vectors does not affect the cell growth rate and their differentiation ability.

Objective To study the effect of red cell and plasma protein parameters on progression, prognosis and recurrence of cerebral infarction. Methods Clinical data from 105 patients with cerebral infarction were analyzed. The pa?tients were divided into four paired groups:progressive stroke group and complete stroke group, short-term favorable progno?sis group and short-term unfavorable prognosis group, long-term favorable prognosis group and long-term unfavorable prog?nosis group, relapsed cerebral infarction group and not relapsed group by different criterion. The red cell and plasma protein parameters were compared between groups. Results There were significantly higher mean corpuscular volume(fL:85.92± 4.50 vs 83.79±4.64,t=2.164,P<0.05), red cell distribution width(fL:13.50±2.45 vs 11.90±2.90,t=2.694,P<0.01), globu?lin(g/L:27.46±4.33 vs 24.79±4.03,t=3.029,P<0.01)and lower albumin(g/L:39.00±3.86 vs 42.89±4.45,t=4.242,P<0.01)in progressive stroke group than those of complete stroke group. The elevated red cell distribution width, reduced albu?min were the risk factors of progressive stroke. In the short-term unfavorable prognosis group, red cell distribution width was significantly higher than that in short-term favorable prognosis group(fL:13.90 ± 2.45 vs 12.00 ± 2.12,t=2.905,P<0.01). The red cell distribution width was positively correlated with mRS scores assessed 3 months and 18 months after cerebral in?farction(P<0.01). Conclusion Progressive stroke rate increases in cerebral infarction patients with elevated red cell distri?bution and reduced albumin;Red cell distribution width has a certain reference value for forecasting the prognosis of cere?bral infarction .