Protein Kinase C (PKC ) was partially purified from rat brain by DEAE-52 column chromatography, and the effects of ethanol extracts of Selaginella doederleinii and Livistona chinensis on PKC activity were investigated.In order to verify the reliability of the assay system, the effect of polymyxin B, a known PKC inhibitor, on the activity of PKC was also tested. Results showed that the ethanol extract of S. doederleinii strongly inhibited PKC activity with IC50 2.2?g/ml. The ethanol extract of L. chinensis (100?g/ml ) inhibited PKC activity by 66.6%. Polymyxin B inhibited PKC activity with IC5017.7 U/ml.

As the student communities develop rapidly,their activities become the new carrier of the ideological and political education work in universities.In his article,the author analyses the current situation,opportunities and challenges exerted by these communities on ideological and political education in universities,and discusses how to enhance the development of student communities from the perspective of ideological and political education as well.

Objective To investigate the different gender and untreated hyperthyroidism parameter difference (hyperthyroidism) and hemoglobin of red blood cells in peripheral blood of patients with relatively healthy people.Methods Collecting blood samples of patients with hyperthyroidism,with EDTA-K2 anticoagulant,using Sysmex XE-2100 automated hematology analyzer analysis of peripheral blood hemoglobin in red blood cell parameters and using healthy people as healthy control.Results Healthy control group,male,female red blood cells and hemoglobin in patients with hyperthyroidism were increased,the difference was statistical-ly significant (P <0.05).Conclusion Newly diagnosed hyperthyroidism value and erythrocyte hemoglobin values higher than nor-mal.

The incidence of cholangiocarcinoma is rising, poor prognosis due to lack of viable treatment.Recent investigations into the underlying molecular mechanisms of cholangiocarcinogenesis and tumor growth have contributed greatly to our understanding of this disease, which will lead to the identification of therapeutic targets for this devastating cancer. The purpose of the following review is to address what has been learned over the past decade concerning the molecular basis of cholangiocarcinogenesis and factors regulating cholangiocarcinoma growth. Through a better understanding of these mechanisms, more specific diagnostic, therapeutic, and preventative strategies may be developed and hopefully improve the outcome of this devastating disease.

OBJECTIVE:To observe the efficacy and safety of low-dose Progesterone injection combined with Baotai wuyou capsule in the treatment of threatened abortion. METHODS:107 patients with threatened abortion were retrospectively analyzed and divided into observation group(54 cases)and control group(53 cases)by different medication. All patients received bed rest orally taking folic acid and other measures. Meanwhile,control group was given Progesterone injection 20-40 mg,qd,intramuscu-larly injected. Observation group was given Progesterone injection 10-20 mg,qd,intramuscularly injected and given 4 Baotai wuy-ou capsule additionally,tid,taking with ginger decoction,no fish during the medication. The courses of 2 groups were 14 d. Clini-cal efficacy,hemostatic time,remission time of abdominal pain and backache in 2 groups were observed,and the incidence of ad-verse reactions during the treatment was recorded. RESULTS:The total effective rate in observation group was significantly higher than control group,hemostatic time,remission time of abdominal pain and backache were significantly shorter than control group, the incidence of adverse reactions was significantly lower than control group,with statistical significances (P<0.05). CONCLU-SIONS:Low-dose Progesterone injection combined with Baotai wuyou capsule shows good efficacy in the treatment of threatened abortion,which can shorten patients'hemostatic time,remission time of abdominal pain and backache,and reduce the incidence of adverse reactions,with good safety.

Side-to-side meso-caval shunt is one of the peripheral porto-systemic shunts that can retain a part of the portal blood flow passing through the hepatic vascular bed. It has the advantages of simple operative maneuver and limited operative traumatism. Furthermore it can maintain the continuity of the inferior vena cave.This type of operation was performed on 24 patients with portal hypertension of different causes from 1963 to October, 1980- There was no immediate operative mortality. One case was complicated with chylous ascites postopera-tively. A second operation failed to correct the condition and the patient died of progressive exhaustion about one month later.Among the follow-up cases, four cases developed hepatoencephalopathy four to seven years later respectively; six cases developed recurrent digestive bleeding within 5 years after the shunt operation.The indications of this operation are: ( 1 ) slender body type;(2)no large amount of ascites; ( 3 ) bleeding after splenectomy; (4) no thrombosis or phlebitis of the superior mesenteric vein;(5)no enlargement of the pancreas; (6) a sufficient length of the main trunk of the superior mesenteric vein; ( 7 ) the distance between the superior mesenteric vein and the inferior vena cava is not too long.If there is a recent history of G-I bleeding, the coronary vein of the stomach should be ligated when the patient is being operated on. Whenever the anatomy of the regional veins is not suitable for this kind of operation, an interposing autogenous internal jugular vein graft had better be used.

Objective To observe the head and neck CTA application value in the diagnosis of ischemic cerebrovascular disease.Methods 430 patients with suspected diagnosis of ischemic cerebrovascular disease were adopted with head and neck CTA examination.The carotid artery stenosis degree,grade and plaque formation were observed,and the sensitivity and specificity of CTA in the diagnosis of ischemic cerebrovascular disease were calculated.Results After CTA examination,a tctal of 293 cases (68.14％) with carotid artery stenosis symptoms,among them 87 cases of mild stenosis,moderate stenosis in 169 cases,35 cases of severe stenosis and occlusion in 2 cases.430 plaques were detected in 430 patients,in extracranial arteries,81 soft plaques were identified,as well as 134 calcified plaque and 107 cases of mixed plaque,totally 322 cases;in intracranial arteries 312 cases of the soft plaque were identified,as well as 115 cases of calcified plaque and 97 cases of mixed plaque,totally 524 cases.The sensitivity of CTA in the diagnosis of ischemic cerebrovascular disease was 97.09％ and the specificity was 83.33％.Conclusion In the diagnosis of ischemic cerebrovascular disease,head and neck CTA examination for head and neck vascular stenosis and plaques has visual assessment,and high sensitivity,specificity.

Objective To explore the correlation of modern medical examination indexes with the traditional Chinese medical syndrome types of reflux esophagitis ( RE) , thus to supply evidence for the standardization of syndrome differentiation. Methods Seventy-six RE patients who met the inclusion criteria were enrolled into the study. And then we investigated the correlation of traditional Chinese medical syndrome types with endoscopicmucosal performance grading, plasma Ghrelin content, esophageal mucosal substance P ( SP) and vasoactive intestinal peptide (VIP) . Results (1) Syndrome patterns of RE showed certain correlation with the endoscopic mucosal performance (P<0.01) . Endoscopic mucosal performance grade 0-Ⅲ corresponded to the syndromes of gallbladder heat attaching stomach, liver-stomach disharmony/phlegm-qi blocking middle energizer, hepatogastric stagnant heat, middle energizer deficiency with reversed flow of qi, respectively. ( 2) The plasma Ghrelin content of RE patients with syndrome of phlegm-qi blocking middle energizer was lower than that of the patients with other syndrome patterns, and the difference was significant compared with the syndromes of hepatogastric sta gnant heat, liver-stomach disharmony, and gallbladder heat attaching stomach ( P<0.05 or P<0.01) . ( 3 ) The expression level of SP in the esophageal mucosa was the highest in the syndrome of hepatogastric stagnant heat, and was the lowest in the syndrome of phlegm-qi blocking middle energizer; expression level of VIP in the esophageal mucosa was the highest in the syndrome of middle energizer deficiency with reversed flow of qi, and was the lowest in the syndrome of hepatogastric stagnant heat ( P<0.05 or P<0.01) . Conclusion Traditional Chinese medical syndrome types of RE have correlation with endoscopic mucosal performance and immunohistochemical detection results for gastrointestinal hormones, which will be beneficial to the estimation of the development and severity of RE.

Proteomics is an emerging branch of bioscience which studies proteome. The main techniques in proteomics include sample preparation in proteomic, two-dimensional gel electrophoresis, two-dimensional difference gel electrophoresis, multidimensional protein identification technology, surface enhanced laser desorption/ionization time-of-flight mass spectrometry. Colorectal cancer is one of the most common cancers worldwide. During the last several years, many advances have been achieved in terms of applications of proteomics in biomarker research for colorectal cancer.

Energy metabolism refers to the metabolism of three major nutrients associated with the process of energy production and usage.The metabolism in patients with tumors manifests specifically, as a result of tumor-bearing state, host's response to tumor and anti-neoplastic therapy.It is believed that resting energy expenditure increases in patients with newly diagnosed tumors, which is related to C-reactive protein and release of inflammatory cytokines. After eflfectjve therapy,resting energy expenditure decreases in some degree.In addition,the stage and differentiation of tumors also affect metabolism.However,there are some conflicts among research findings.The characteristics of energy metabolism and influential factors in patients with tumors are reviewed in this paper.