Objective To establish a cell line and a convenient and well-duplicated minimal residual leukemia (MRL) model. Methods We chose the international standard domestic inbred DBA/2 mouse to inoculate the different numbers of L1210 leukemic cells to investigate the relationship between inoculating cell number and mouse survival time. On the third day after 1×106 leukemic cells per mouse were inoculated, different doses of CTX were used for the chemotherapy. Results The inoculating number of L1210 leukemic cells increased while the mouse survival time decreased;The survival time increased following the increase of the doses of the CTX;The survival time of the inoculated mouse treated with CTX at 125 mg/kg was equal to that of the mouse inoculated with 500 L1210 leukemic cells without treatment. Conclusion The observation results are that the dose of 125 mg/kg for creating the model of MRL mouse.

BACKGROUND:For distal clavicle fracture, clavicular hook plate and Kirschner wire tension band were used in the clinic, but they cannot keep the dual function of stability and functional protection. However, the application of microporous locking plate of distal clavicle makes it possible to double efficacy. OBJECTIVE:To compare and analyze the clinical outcomes of distal clavicle microporous locking plate and clavicular hook plate. METHODS:From March 2011 to March 2013, 40 patients with distal clavicle fractures were involved in this study. They were divided into two groups with the voluntary principles:distal clavicle microporous locking plate group and clavicular hook plate group. We compared and observed perioperative indicators (operation time, blood loss, and hospitalization days), clinical curative effects (University of California at Los Angeles scoring system and American Shoulder and Elbow Surgeons Scale), the score of shoulder joint function (Karlsson standard) and the overal treatment (removed the plate and functional training costs). RESULTS AND CONCLUSION:Al patients successful y underwent surgery. At 1 year after surgery, internal fixators were taken out. Necessary relevant rehabilitation training was performed for more than 3 months. There was no significant difference in perioperative indicators (P>0.05). However, clinical curative effects and the score of Karlsson shoulder joint function showed significant advantages in the microporous locking plate group (P<0.05). Although the distal clavicle microporous locking plate material cost was high, but there was no significant statistic difference in the overal treatment for better protection shoulder function (P>0.05). We draw the conclusion that distal clavicle microporous locking plate can effectively reconstruct the stability of the fracture site, and better retain the function of the joints at the same time.

Chronic kidney disease(CKD)is increasingly recognized as a global public health problem.Uncontrolled complications of CKD,especially cardiovascular diseases,contribute greatly to the premature death and unfavorable prognosis.Recent evidence shows that CKD complications may occur earlier than previously thought.CKD complications deserve early detection and active treatment.Periodical follow-up and regular check should be done to adjust the therapeutic condition.Clinical practice guideline or recommendation based on evidence-based medicine is essential for management of CKD complications.Personalized treatment should be considered to improve survival and quality of life,and to make patient return to society.

Objective To evaluate whether the pentylenetetrazol(PTZ)model is an appropriate animal model of absence seizure.Methods A series of tests including EEG,active avoidance response(AAR)and passive escape response(PER)in a two-way shuttle box were finished during seizure induced by PTZ(35 mg/kg,ip)in 40 Wistar epileptic rats.Results Epileptiform activity was characterized by bilaterally spike wave discharges synchronously elicited during seizure in PTZ models.During absence seizure induced by PTZ,the latency of active avoidance response was significantly prolonged.AAR positive rate was decreased by 85%.PER positive rate was increased by 56%,and escape failure positive rate was increased by 29%.Conclusion There is epileptiform discharge accompanying transient conscious disturbance during absence seizure induced by pentylenetetrazol.

Objective To summarize the clinical experience of laparoscopic cholecystectomy (LC) for acute cholecystitis. Methods Clinical records of 79 cases of acute cholecystitis treated by LC from September 2002 to August 2003 were respectively reviewed. Results Intraoperative cholangiography (IOC) was successfully conducted in 75 cases, 6 of which were found common bile duct stones. Of the 6 cases, 4 underwent LC combined with intraoperative endoscopic sphincterotomy and 2 underwent a conversion to open choledochotomy with T-tube drainage. The rest of 73 cases were diagnosed as simple gallbladder stones: LC was successfully accomplished in 70 cases and a conversion to open surgery was required in 3 cases because of serious inflammatory adhesion. In the study no severe complications occurred. Conclusions LC can be performed safely in the majority of cases of acute cholecystitis.

Objective To summarize the clinical effects of retrograde intramedullary nail in treating distal femora| fracture. Methods Retrospectively analysed 52 cases of intereondyiar and supracondylar fracture of femur treated with retrograde intrameduUary nail. Results All cases were followed up for 6 to 24 months. All fracture were union except 2 reoperations cases had delay union. Conclusion Retrograde intrameduUary nail has supreme biological stability and allowed early exercise. It also can shorten operation time, reduce damage of periosteum and reduce delay union rate.

Objective To observe the influence of self-management on health care behavior through health education for patients with hypertension after a self-management model was brought to the communities. Methods There are 252 cases chosen randomly from 546 hypertensive patients in 19 communities to participate in the standard management of hypertension in communities voluntarily. They improve their self-management skills through attending lectures, the guidance of healthy lifestyle and self-management manuals. A scientific self-management methods was established and the support of the society and family was improved. The self-control method was used to analyze this study. Results There are significantly differents (P<0.05 ) in basic health knowledge understanding, self-management skills, and blood pressure control after health education compared with before. Conclusions Patients with health education can improve the self-management level. There are obvious effects on changing the bad lifestyle,improving blood pressure control rate, and decreasing the complication of patients with hypertension.

Gastric cancer(GC) is deeply threatening the health of our people, the mortality rate of GC is the second death cause of carcinoma i n our country. Since the lack of special clinical symptom at the early state of GC, detecting GC at the molecular biological level is expected to diagnose early gastric cancer. This article discusses some markers such as PCNA, p53, telomera se, ras protein and so on to assess the possibility of diagnosing the early gas tric cancer with these molecular biological markers. According to analyzing rela ted papers published, PCNA and p53 were found to be the molecular biological mar kers which should be firstly taken into account in diagnosing gastric cancer. Bo th of them can be easily detected by using immunohistochemical method. Telomeras e is also a good marker, but because of its difficult technology and high cost, it is not so practicable as the former two as the diagnostic marker of early gas tric cancer. Besides, ras potein can also be taken into account as the early det ecting auxiliary marker for GC.