OBJECTIVES: To evaluate the protective effects of glutathione (GSH) on the cytotoxicity of mercurial compounds(CH3HgCl, HgCl2) or cadmium chloride(CdCl2) in EMT-6 cells. METHODS: The compounds investigated were CH3HgCl, HgCl2, CdCl2, GSH, buthionine sulfoximine(BSO), L-2-oxothiazolidine-4-carboxylic acid(OTC). Cytotoxicity analysis consist of nitric oxide(NO) production, ATP production and cell viability. RESULTS: Mercurial compounds and cadmium chloride significantly decreased cell viability and the synthesis of NO and cellular ATP in EMT-6 cells. GSH was not toxic at concentrations of 0 - 1.6 mM. In the presence of GSH, mercurial compounds and cadmium did not decrease the production of ATP and nitrite in EMT-6 cells. The protective effects of GSH against the cytotoxicity of mercurial compounds and cadmium depended on the concentration of added GSH to the culture medium for EMT-6 cells. We evaluated the effects of intracellular GSH level on mercury- or cadmium-induced cytotoxicity by the pretreatment experiments. Pretreatment of GSH was not changed NO2- and ATP production, and pretreatment of BSO was decreased in dose- and time-dependent manner. Pretreatment of OTC was increased NO2- and ATP production in dose- and time-dependent manner. Because intracellular GSH level was increased by OTC pretreatment, the protective effect on mercury- and cadmium-induced cytotoxicity was increased. CONCLUSIONS: These results indicated that sulfhydryl compounds had the protective effects against mercury-induced cytotoxicity by the intracellular GSH levels.
Adenosine Triphosphate ; Cadmium Chloride ; Cadmium* ; Cell Survival ; Glutathione* ; Mercuric Chloride ; Nitric Oxide ; Sulfhydryl Compounds

Effect of cadmium (Cd) intoxication on renal acid-base regulation was studied in adult male Sprague-Dawley rats. Cd intoxication was induced by subcutaneous injections of CdCl2 at a dose of 2 mg Cd/kg/day for 3-4 weeks. In Cd-intoxicated animals, arterial pH, PCO2 and plasma bicarbonate concentration decreased, showing a metabolic acidosis. Urine pH and urinary bicarbonate excretion increased and titratable acid excretion decreased with no change in ammonium excretion. In renal cortical brush-border membrane vesicles derived from Cd-exposed animals, the Na /H antiporter activity was significantly attenuated. These results indicate that chronic exposures to Cd impair the proximal tubular mechanism for H secretion (i.e., Na /H antiport), leading to a metabolic acidosis.
Acidosis ; Acidosis, Renal Tubular* ; Adult ; Ammonium Compounds ; Animals ; Cadmium ; Cadmium Chloride ; Humans ; Hydrogen-Ion Concentration ; Injections, Subcutaneous ; Ion Transport ; Kidney ; Male ; Membranes ; Plasma ; Rats* ; Rats, Sprague-Dawley

Twenty-four factories registered at the Ministry of Labor as factories using or producing cadmium in 1991 were investigated to know the present state of occupational exposure to cadmium in 1992. Nine factories among 24 stopped using or changed to other substances and 1 factory didn't produce any more, but delivered it to other factories through imports. Therefore, only 14 factories were producing or using cadmium. But, alloying materials were uspplied to about 20 and more factories, however, they couldn't be investigated because of limited information. Three factories had less than 50 workers were 3, 6 had 50-300 and 5 had more than 300. About 167 workers were exposed to cadmium. According to the classification by process, there were 4 alloy processes, 2 refinary, 2 elctroplating, 2 dry cell making, 1 industrial battery and 1 copper refinary. 8 factories used metal cadmium, 3 cadmium oxide, 2 cadmium sulfide, and 1 cadmium hyroxide. One factory was importing cadmium stearates only and supplying them to the PVC making factories. The amount of metal cadmium producing in 2 refinary factories was about 50-65 ton per month. The other cadmium compounds were imported. According to exposure level of cadmium in air of the 10 factories examined, each factory could be divided into high, moderate and low exposure groups. 1 battery recycling factory and 3 alloy factories were in the high exposure group and their mean air concentrations were 0.06679, 0.05481, 0.02492, and 0.003mg/m3, respectively. Refinary and industrial battery making factories were in the middle exposure group and their mean air concentrations were 0.00773 and 0.00613 mg/m3, respectively. Finally, low exposure group were copper smelting, dry cell case making, duplicator drum making and electroplating factory and their mean air concentrations were 0.00096, 0.00071, 0.00057 and 0.00054 mg/m3 respectively.
Alloys ; Cadmium Compounds ; Cadmium* ; Classification ; Copper ; Electroplating ; Korea* ; Occupational Exposure* ; Osmeriformes ; Recycling ; Stearates

Semiconductor nanoparticles generate photoelectrons and photo-induced holes under light excitation, and thus may influence the growth of microbial cells. The highly oxidative holes may severely damage the cells, while the photoelectrons may promote microbial metabolism. In this study, we evaluated the effect of exogenous cadmium sulfide (CdS) nanoparticles on bacterial growth using OD₆₀₀ and colony forming unit (CFU) as indicators. The oxidase activities, the concentration of pyruvate and malondialdehyde, and the expression of relevant genes assessed by real-time fluorescent quantitative PCR were analyzed to investigate the effect of excited CdS on cellular metabolism. The results showed that the OD₆₀₀ and pyruvate accumulation of E. coli increased by 32.4% and 34.6%, respectively, under light conditions. Moreover, the relative expression level of the division protein gene ftsZ was increased more than 50%, and the tricarboxylic acid cycle pathway gene icdA and gltA increased by 86% and 103%, respectively. The results indicated that photoelectrons could be used by microorganisms, resulting in promoted growth and metabolism. This study gives a deep insight into the interaction between nanoparticles and bacteria.
Escherichia coli/metabolism* ; Nanoparticles ; Cadmium Compounds/metabolism* ; Quantum Dots

Breast Neoplasms ; metabolism ; pathology ; Cadmium Compounds ; Cell Line, Tumor ; Female ; Humans ; Quantum Dots ; Receptor, ErbB-2 ; analysis ; metabolism ; Selenium Compounds ; Spectrometry, Fluorescence ; Tellurium ; Zinc Compounds

OBJECTIVETo investigate the effects of nanosized cadmium sulfide (nano-CdS) on the male reproductive system in mice.

METHODSThirty-six specific pathogen?free male ICR mice were equally and randomly divided into three groups: two experimental groups and a control group. The two experimental groups were exposed to 100 mg/kg and 50 mg/kg nano-CdS once daily by gavage, respectively, while the control group was exposed to the same volume of physiological saline once daily by gavage. After 45 days, levels of cadmium accumulation and serum testosterone in the testis were determined, the epididymal sperm count, the rate of sperm abnormality, and histopathological changes in testis tissue were observed under a microscope, and the level of CYP11A1 mRNA was determined by qRT-PCR.

RESULTSCompared with the control group, the two experimental groups had a significantly increased level of cadmium accumulation in the testis (P < 0.05), and the 100 mg/kg nano-CdS group had a significantly decreased epididymal sperm count (P < 0.05) and a significantly increased rate of sperm abnormality (P < 0.05), but the 50 mg/kg nano-CdS group did not. The 100 mg/kg nano-CdS group showed different histopathological changes in testis tissue, but the 50 mg/kg nano-CdS group did not. The two experimental groups had significantly reduced levels of testosterone and CYP11A1 mRNA compared with the control group.

CONCLUSIONNano-CdS given through the digestive tract may have an effect on the male reproductive system in mice by affecting the key enzyme genes in the androgen synthesis pathway to reduce the levels of reproductive hormones.

Animals ; Cadmium ; Cadmium Compounds ; toxicity ; Gonadal Steroid Hormones ; Male ; Mice ; Mice, Inbred ICR ; Sperm Count ; Spermatozoa ; Sulfides ; toxicity ; Testis ; drug effects ; Testosterone

OBJECTIVETo synthesize some new alpha-mercapto-beta-substituted aryl acrylic acids, characterize them and investigate their in vitro cadmium chelating ability.

METHODSSix alpha-mercapto-beta-substituted aryl acrylic acids were prepared by the alkaline hydrolysis of 5- (aryl methylene) rhodanines, obtained from the condensation of substituted aldehydes and rhodanine following the reported procedure. The new compounds were characterized by elemental analysis, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The liver and kidney from cadmium chloride pre-administered rats were homogenized and their nuclear mitochondrial fraction (NMF) and supernatant cytosol fraction (SCF) were separated. A measured volume of each fraction was dialyzed separately using "dialysis sack" against buffered-KCl medium containing a compound in the final concentration of 1 x 10(-3) mol/L for 3 h at 37 degrees C. The whole content of "sack" was subjected to cadmium estimation following digestion with conc. Nitric acid was detected using flame atomic absorption spectrometer.

RESULTSThe in vitro screening showed that alpha-mercapto-beta-(p-methoxyphenyl) acrylic acid (compound 2) and alpha-mercapto-beta-(m-methoxy, p-hydroxyphenyl) acrylic acid (compound 4) were more effective than alpha-mercapto-beta-thienyl acrylic acid (compound 1) and alpha-mercapto-beta-(p-dimethylaminophenyl) acrylic acid (compound 3) in mobilizing cadmium as their dialyzable chelates. The presence of a methoxy group on the phenyl moiety (compounds 2 and 4) increases the metal chelating ability of mercapto acrylic acids.

CONCLUSIONSCompounds 2 and 4 seem to have accessibility to the cellular system and capability of chelating-out the intracellularly bound cadmium.

Acrylates ; chemical synthesis ; chemistry ; pharmacology ; Animals ; Cadmium Chloride ; metabolism ; toxicity ; Chelating Agents ; chemical synthesis ; chemistry ; pharmacology ; Injections, Intraperitoneal ; Kidney ; drug effects ; metabolism ; Liver ; drug effects ; metabolism ; Male ; Mitochondria ; drug effects ; metabolism ; Mitochondria, Liver ; drug effects ; metabolism ; Rats ; Sulfhydryl Compounds ; chemical synthesis ; chemistry ; pharmacology

N-acetyl-L-cysteine (NAC) capped quantum dots (QDs) were synthesized by a hydrothermal method and coated with 2-amino-2-deoxy-D-glucose (DG), polyethylene glycol (PEG), and 9-D-arginine (9R). The optical properties, morphology and structure of 9R/DG-coated CdTe QDs were characterized by ultraviolet-visible spectrometry, fluorescence spectrum, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H NMR), liquid chromatography-mass spectrometer (LC-MS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transmission electron micrographs (TEM). Furthermore, the biocompatibility, tumor targeted ability and transmembrane action of 9R/DG-coated CdTe QDs were studied. Results indicated that 9R/DG-coated CdTe QDs was constructed successfully by ligand exchange. The 9R/DG-coated CdTe QDs with the size of 8-10 nm had good dispersity and the absorbance and fluorescence peaks of CdTe QDs after modification were red shifted from 480 nm to 510 nm and 627 nm to 659 nm, respectively. In addition, the CdTe QDs modified by PEG, DG and 9R displayed good biocompatibility, high targeted ability to the cancer cells with glucose transporter type 1 (GLUT1) receptor high expression and obvious transmembrane ability.
Acetylcysteine ; chemistry ; Cadmium Compounds ; pharmacology ; Humans ; Neoplasms ; drug therapy ; Polymers ; chemistry ; Quantum Dots ; chemistry ; Spectrophotometry, Ultraviolet ; Tellurium ; pharmacology

Adolescent ; Adult ; Air Pollutants, Occupational ; adverse effects ; analysis ; Cadmium Compounds ; adverse effects ; analysis ; Eye ; drug effects ; Humans ; Oxides ; adverse effects ; analysis ; Young Adult

Animals ; Cadmium Compounds ; toxicity ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cricetinae ; Cricetulus ; Fibroblasts ; drug effects ; metabolism ; Lactate Dehydrogenases ; metabolism ; Lung ; cytology ; Malondialdehyde ; metabolism ; Quantum Dots