Objective:To evaluate the therapeutic effect of combined procedure of surgical left ventricular repair (LVR) and skeletal myoblast transplantation for congestive heart failure after myocardial infarction. Methods: Forty-eight male adult Lewis rats, with a four-week old myocardial infarction created by left coronary artery ligation, were randomized into 4 groups: group Ⅰ (n=10) received phosphate-buffered saline (PBS) injection into the border zone of the infarction; group Ⅱ(n=11) received myoblast injection ; group Ⅲ(n=13) received left ventricular repair and simultaneous PBS injection; and group Ⅳ (n=14) received left ventricular repair and myoblast injection. Echocardiography was performed once a week until the fourth week after the treatment. Results: Left ventricle end diastolic diameter (EDD) and end systolic diameter (ESD) declined significantly one week after the LV repair in group Ⅲ and Ⅳ, then increased as time passed; on the fourth week, there were no statistical differences between group Ⅰ,Ⅱ and Ⅲ, but group Ⅳ was still much smaller than the other 3 groups(P

Objective To observe the effect of laparoscopy combined with choledochoscopy in treatment of cholelithiasis clinical effect .Methods 80 cases diagnosed as cholelithiasis cases were divided into control group and observation group with 40 cases in each group .The control group used the traditional laparotomy incision gallbladder stone ;observation group were treated by laparos-copy combined with choledochoscopy .Results In the observation group ,the operation and the hospital stay were significantly shor-ter than the control group ,operation curative effect was obviously superior to the control group ,the incidence of postoperative com-plications was significantly lower than the control group .Conclusion The use of laparoscopy combined with choledochoscopy tech-nology in treatment of gallstones have good clinical effect .

BACKGROUND:Recent studies have shown that platelet rich plasma has strong osteogenic ability and it has been widely used in the field of department of orthopedics and department of stomatology, but experimental study on avascular necrosis of the femoral head has been not reported.
OBJECTIVE:To observe the effect of platelet rich plasma gel combined with marrow core decompression in the repair of aseptic necrosis of rabbit femoral head.
METHODS:A total of 25 New Zealand white rabbits were obtained. Without dislocation of the hip joint, aseptic necrosis model was established by using liquid nitrogen cryotherapy on bilateral femoral head of rabbits. At 2 weeks after model establishment, rabbit models were randomly assigned. Model group with nine rabbits did not receive any treatment. Core decompression group with eight rabbits underwent core decompression treatment. Combination group with eight rabbits underwent core decompression combined with platelet rich plasma gel treatment. At 8 weeks after model induction, tissue sections of femoral head specimens were col ected and subjected to MRI and hematoxylin-eosin staining.
RESULTS AND CONCLUSION:(1) MRI examination:The model group showed low signal area of different forms in femoral head fat high signal, exhibited annular, ribbon and focal shapes. No evident changes in osteogenesis, no apparent minification of the defect cavity were detected at defect site in the core decompression group, showing long T1 and T2 signals. Defect cavity disappeared, and new bone of short T1 and T2 signals fil ed in the combination group. (2) Hematoxylin-eosin staining:Articular cartilage defect and repair existed in the model group, showing osteoarthritis-like changes. Empty bone lacuna of bone trabecula, hematopoietic celland adipocyte necrosis in bone marrow were detected in the core decompression group. Empty bone lacuna of bone trabecula, focal necrosis scattered in bone marrow were visible in the combination group. Results suggested that the outcomes of platelet rich plasma gel combined with core decompression were better than core decompression alone in treatment of rabbit models of femoral head necrosis.

Nucleic acids, as a next generation of biotechnology drugs, not only can fundamentally treat diseases, but also own significant platform characteristics in view of technology and production. Therefore, nucleic acid-based drugs have broad clinical applications in biomedical fields. However, nucleic acids are degradable and unstable, and have very low intracellular delivery efficiency in vitro and in vivo, which greatly limits their applications. In recent years, ionizable lipid-based lipid nanoparticles have shown promising application potentials and have been successfully applied to COVID-19 (Coronavirus Disease 2019) vaccines in clinic. Lipid nanoparticles demonstrate high in vivo delivery efficiency and good safety profile due to their unique structural and physicochemical properties, which provides many possibilities for their clinical applications for nucleic acid delivery in the future. This review focused on the characteristics of nucleic acid drugs and their delivery barriers, and discussed the approved nucleic acid drugs to illustrate the key aspects of the success of their delivery carrier system. In addition, problems to be solved in the field were highlighted.

Atrial Fibrillation ; surgery ; Catheter Ablation ; methods ; Humans

Acupuncture Therapy ; Child, Preschool ; Gait Disorders, Neurologic ; etiology ; physiopathology ; therapy ; Hand, Foot and Mouth Disease ; complications ; virology ; Humans ; Male ; Walking

objective to find a proper pathway for the regional chemotherapy of pancreatic carcinoma. Method A total of 39 cases of unresectable pancreatic carcinoma was collected and divided into five groups. Ⅰ: celiac artery group, 5 cases; Ⅱ: gastroduo-denal artery group,24 cases;15 cases were directly inserted into this artery (ⅡB)and 9 cases indirectly through gastroepiploric artery (ⅡA). Ⅲ: gastroepiploric right artery group, Scases; Ⅳ: splenic artery group, 5 cases. Result. For these groups, the first group can be detected that the pancreatic head is clearly dyed; in the second group the pancreatic head was apparently dyed. The body and tail of pancreas were clearly dyed in the splenic artery group and failed in the second group. Conclusion For pancreatic head carcinoma, the gastroduodenal artery is the first choice; splenic artery is proper for the carcinoma in the body and tail of pancreas.

Objective: To observe influence of hypotension induced with diethylamine(DEA)on oxygen delivery (DO_2), oxygen consumption (VO_2)and the concentration of lactate. Method: Twelve healthy adult dogs were randomly assigned into one of the two groups. 0.001% DEA or 0.0l% sodium nitroprusside (SNP) was infused to induce mean arterial pressure(MAP) to decrease to 60% of baseline and maintaine for 30 min. During hypotension, MAP and HR were recorded. Arterial and mixed venous blood gas, and blood lactate level were measured. Result: There were no sig nificant changes in DO_2. VO_2, oxygen extraction ratio and blood lactate level in both groups. The concentration of lac tate was not increased in either group. Conclusion. The oxygen metabolism may not be affected during DEA-induced hypotention

Objective To investigate the effects of rutaecarpine on the cerebral injury and calcitonin gene-related peptide (CGRP) level during focal cerebral ischemia-reperfusion injury in rats. Methods Rats were intravenously given three doses of rutaecarpine (50, 100 and 300?g/kg) or vehicle 30 minutes before experiment. Then the rats subjected middle cerebral artery occlusion (MCAO) for 2 hours, and neurological deficits scores were performed at 6, 12 and 24 hours after reperfusion. After the last test the animals were sacrificed, the infarct volumes were determined by TTC staining, and CGRP levels were measured by radioimmuoassay. Results Rutaecarpine significantly reduced infarct volume, and improved cerebral function in dose-dependent manner compared with vehicle. Rutaecarpine significantly inecreased brain CGRP levels after reperfusion as well. Conclusion Rutaecarpine has marked protective effects on ischemic brain damage in rats possibly by increasing CGRP release in the brain.

Objective To investigate the effects of different concentrations of sevoflurane and desflurane on ultrastructure of the lung and pulmonary surfactant-related protein A (SP-A) and phosphatidylcholine (PC) concentrations in broncho-alveolar lavage fluid ( HALF). Methods Fifty healthy Wistar rats of either sex weighing 200-280 g were randomly divided into 5 groups (n = 10 each) : group Ⅱ control; group Ⅱ sevoflurane 1.0 MAC (S1); group Ⅲ sevoflurane 1.5 MAC (S2); group Ⅳ desflurane 1.0 MAC (D1) and group Ⅴ desflurane 1.5 MAC (D2). The animals were anesthetized with intraperitoneal pentobarbital 100 mg?kg-1 , tracheotomized and mechanically ventilated (VT = 8 ml?kg-1 , RR = 65-70 bpm) . PETCO2 was maintained at 35-40 mm Hg. In control group the animals received mechanical ventilation for 4 h without any inhalation anesthetic. In group Ⅳ - Ⅴ sevoflurane or desflurane anesthesia was maintained for 4 h. The animals were then killed and the lungs removed for microscopic examination with electron microscope and broncho-alveolar lavage. The HALF was collected for determination of SP-A and PC concentrations. Results In control group the type Ⅱ alveolar epithelial cells were round or oval in shape, abundant in cytoplasma. The microvilli were only slightly destroyed.The lamellar bodies were structurally intact and concentrically arranged. While in group S1, S2, D1 and D2 the microvilli were destroyed to a greater degree and became shorter. The lamellar bodies were decreased in number with many vacuoles. The SP-A and PC concentrations in BALF were significantly decreased in group S1, S2, D1 and D2 as compared to control group. Conclusion Inhalation of different concentrations of isoflurane or desflurane for 4 h reduces the number of lamellar bodies with vacuolization and SP-A and PC concentrations in BALF indicating decreased synthesis of pulmonary surfactant.